RESUMEN
BACKGROUND: Litsea glutinosa (Lour.) C. B. Rob. belongs to the Litsea genus and is categorized under the family of Lauraceae. The study aimed to investigate the phytoconstituents and pharmacological properties of methanol extract of leaves of Litsea glutinosa, focusing on antidiabetic activity via in vivo and in silico techniques. METHODS: Extensive chromatographic and spectroscopic techniques were applied to isolate and characterize the constituents from the L. glutinosa plant species. The antidiabetic activity was studied in streptozotocin-induced diabetes mice, and the computational study of the isolated compounds was carried out by utilizing AutoDock Vina programs. In addition, the pharmacokinetic properties in terms of absorption, distribution, metabolism and excretion (ADME) and toxicological profiles of the isolated compounds were examined via in silico techniques. RESULTS: In the present study, two flavonoid glycosides 4Î-O-methyl (2 Ì,4 Ì-di-E-p-coumaroyl) afzelin (1) and quercetin 3-O-(2 Ì,4 Ì-di-E-p-coumaroyl)-α-L-rhamnopyranoside (2) were isolated from the leaves of L. glutinosa and characterized by 1H and 13C NMR, COSY, HSQC, HMBC, and mass spectral data. Although compounds 1 and 2 have been reported twice from Machilis litseifolia and Lindera akoensis, and Machilis litseifolia and Mammea longifolia, respectively, this is the first report of this isolation from a Litsea species. Administering the methanolic extract of L. glutinosa at doses of 300 and 500 mg/kg/day to mice with diabetes induced by streptozotocin led to a significant decrease in fasting blood glucose levels (p < 0.05) starting from the 7th day of treatment. Besides, the computational study and PASS analysis endorsed the current in vivo findings that the both isolated compounds exerted higher binding affinities to human pancreatic α-amylase and aldose reductase than the conventional drugs. The in silico ADMET analysis revealed that the both isolated compounds have a favorable pharmacokinetic and safety profile suitable for human consumption. CONCLUSION: According to the current outcomes obtained from in vivo and in silico techniques, the leaf extract of L. glutinosa could be a natural remedy for treating diabetes, and the isolated phytoconstituents could be applied against various illnesses, mainly hyperglycemia. However, more investigations are required for extensive phytochemical isolation and pharmacological activities of these phytoconstituents against broader targets with exact mechanisms of action.
Asunto(s)
Diabetes Mellitus , Litsea , Humanos , Animales , Ratones , Flavonoides/química , Glicósidos/farmacología , Litsea/química , Hipoglucemiantes/farmacología , EstreptozocinaRESUMEN
Four new compounds (derriscandenon D (1), E (2), F (3), G (4)) and six known isoflavones (warangalone (5), millewanin E (6), rhynedlin A (7), 6,8-diprenylgenistein (8), isolupalbigenin (9), isoscandinone (10)) were isolated from the acetone extract of the branches of Derris scandens. These compounds were assayed for cell viability using the human lung carcinoma cell line A549, colorectal carcinoma cell line Colo205, epidermoid carcinoma cell line KB, the human acute lymphoblastic leukaemia cell line NALM-6, and human dermal fibroblasts. Compounds 2 and 3 significantly decreased the viability of KB cells, with IC50 values of 2.7 and 12.9 µM, respectively. In addition, compounds 2 and 3 reduced the mitochondrial membrane potential in KB cells. Compounds 2 and 3 strongly down-regulated the cell viability of cell lines KB and NALM-6, achieving IC50 values of 2.7 and 0.9 µM, respectively, compared with the positive control staurosporine at 1.25 and 0.01 µM, respectively.
Asunto(s)
Derris , Isoflavonas , Supervivencia Celular , Isoflavonas/farmacología , Potencial de la Membrana Mitocondrial , Extractos VegetalesRESUMEN
Three undescribed isoflavones, derriscandenon A, B, and C, together with seven known isoflavones were isolated and structurally characterized during a study of the chemical constituents in the leaves of Derris scandens (Roxb.) Benth (Leguminosae, Fabaceae) collected in Bangladesh. The inhibitory activity of the compounds against activation of Epstein-Barr virus antigen (EBV-EA) by 12-O-tetradecanoylphorbo-13-acetate (TPA) was measured to identify possible chemopreventive agents. Mild inhibitory effects (IC50 278-290 mol ratio/32 pmol TPA) against EBV-EA induction compared with curcumin (IC50 341 mol ratio/32 pmol TPA) were observed for four known compounds (lupalbigenin, isopalbigenin, glyurallin, and isangustone A). Next, we focused on antitumor effects and investigated cell viability, cell proliferation, and mitochondria membrane potential by using an MTT assay, a live cell monitoring system, and fluorescence staining. Of the seven isoflavones tested for cell viability, a dose-dependent decrease in cell viability was observed for four isoflavones (derriscandenon B and C, derrubone, and glyurallin) in KB cells and two compounds (derriscandenon B and isochandaisone) in NALM6-MSH+ cells. In addition, the proliferation of KB cells was significantly inhibited by these four compounds at a concentration of 5 µM. The mitochondria membrane potentials of KB cells treated with derriscandenon C, derrubone, and glyurallin at the IC50 concentration were decreased by about 55%, whereas undescribed compound derriscandenon B had no effect. Our results show that some of the compounds isolated from D. scandens may be suitable as seed compounds for cancer prevention and therapy.
Asunto(s)
Derris , Fabaceae , Isoflavonas , Neoplasias , Bangladesh , HumanosRESUMEN
OBJECTIVES: The aim of this study was to identify the predictors of side branch (SB) ostial stenosis developed after provisional stenting of the main vessel (MV) using optical coherence tomography (OCT). BACKGROUND: Provisional stenting remains the main approach to treatment of bifurcation lesions; however, it may result in the narrowing of SB ostium. There is little information about underlying plaque morphology of the MV lesion and its potential impact on the SB after provisional stenting. METHODS: Patients with stable coronary disease with angiographic MV lesion not involving SB were included in a prospective single center study. The primary outcome was significant SB ostium stenosis (SBOS), defined as residual stenosis of >50% after MV stenting. RESULTS: Thirty bifurcation lesions in 30 patients were analyzed in the study. Poststenting significant SBOS was observed in 30% of patients. The MV lesions with SBOS > 50% were characterized by a higher prevalence of lipid rich plaques (100 vs. 64%, p = 0.040) and spotty calcifications (60 vs. 0%, p = 0.005). Maximal lipid arcs were greater (257° vs. 132°, p = 0.001) and lipid volume index was higher (1380 vs. 574, p = 0.012) in the SBOS >50% group. Multivariate logistic regression analysis identified maximal lipid arc (odds ratio (OR): 1.014, p = 0.038) and the presence of lipid plaque contralateral to SB ostium (OR: 8.14, p = 0.046) before stenting as independent predictors of significant SBOS after PCI. CONCLUSIONS: High lipid content of the MV lesion and a contralateral location of lipid in the bifurcation area may contribute to SBOS after provisional stenting. © 2016 Wiley Periodicals, Inc.
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Enfermedad de la Arteria Coronaria/terapia , Oclusión Coronaria/diagnóstico por imagen , Estenosis Coronaria/terapia , Vasos Coronarios/diagnóstico por imagen , Stents Liberadores de Fármacos , Intervención Coronaria Percutánea/instrumentación , Placa Aterosclerótica , Tomografía de Coherencia Óptica , Anciano , Distribución de Chi-Cuadrado , Angiografía Coronaria , Enfermedad de la Arteria Coronaria/diagnóstico por imagen , Enfermedad de la Arteria Coronaria/metabolismo , Enfermedad de la Arteria Coronaria/patología , Oclusión Coronaria/etiología , Oclusión Coronaria/patología , Estenosis Coronaria/diagnóstico por imagen , Estenosis Coronaria/metabolismo , Estenosis Coronaria/patología , Vasos Coronarios/química , Vasos Coronarios/patología , Femenino , Humanos , Lípidos/análisis , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Ciudad de Nueva York , Oportunidad Relativa , Intervención Coronaria Percutánea/efectos adversos , Valor Predictivo de las Pruebas , Estudios Prospectivos , Factores de Riesgo , Factores de Tiempo , Resultado del TratamientoRESUMEN
Radial access for percutaneous coronary intervention (PCI) has been shown to reduce mortality and vascular complications compared to femoral access in patients with ST-segment elevation myocardial infarction. However, efficacy and safety of radial access PCI in non-ST-segment elevation acute coronary syndrome (NSTE ACS) is not well understood. A systematic search of electronic databases was performed through July 2015 to search and identify relevant studies. We evaluated the following short-term outcomes: all-cause mortality, major bleeding, access site bleeding, and need for blood transfusions. In addition, we evaluated 1-year mortality. Studies were pooled using random effects model. Nine studies including a total of 220,126 patients (radial approach: 94,663 patients [43%], femoral approach: 125,463 patients [57%]) were included in the analysis. On pooled analysis, no significant difference in incidence of short-term all-cause mortality was found between radial and femoral access (odds ratio [OR] 0.78, 95% CI 0.57 to 1.07, p = 0.12). Radial access was associated with significant reduction in major bleeding (OR 0.52, 95% CI 0.36 to 0.73, p = 0.0002), access-site bleeding (OR 0.41, 95% CI 0.22 to 0.78, p = 0.007), and need for blood transfusions (OR 0.61, 95% CI 0.41 to 0.91, p = 0.02). Furthermore, the 1-year mortality was significantly lower in radial approach (OR 0.72, 95% CI 0.55 to 0.95, p = 0.02). In conclusion, in patients with non-ST-segment elevation acute coronary syndrome undergoing PCI, radial access is associated with decreased bleeding and access-site complications.
Asunto(s)
Síndrome Coronario Agudo/cirugía , Cateterismo Periférico/métodos , Intervención Coronaria Percutánea/métodos , Electrocardiografía , Arteria Femoral , Humanos , Arteria Radial , Resultado del TratamientoRESUMEN
The motility of zoospores is critical in the disease cycles of the peronosporomycetes that cause devastating diseases in plants, fishes, vertebrates, and microbes. In the course of screening for secondary metabolites regulating the motility of zoospores of Phytophthora capsici, we discovered two new inhibitors from the ethyl acetate extract of the fermentation broth of a marine-derived strain Bacillus sp. 109GGC020. The structures of these novel metabolites were elucidated as new cyclic lipopeptides and named gageopeptins A (1) and B (2) by spectroscopic analyses including high resolution MS and extensive 1D and 2D NMR. The stereoconfigurations of 1 and 2 were assigned based on the chemical derivatization studies and reviews of the literature data. Although compounds 1 and 2 impaired the motility of zoospores of P. capsici in dose- and time-dependent manners, compound 1 (IC50 = 1 µg/ml) was an approximately 400-fold stronger motility inhibitor than 2 (IC50 = 400 µg/ml). Interestingly, the zoospores halted by compound 1 were subsequently lysed at higher concentrations (IC50 = 50 µg/ml). Compounds 1 and 2 were also tested against some bacteria and fungi by broth dilution assay, and exhibited moderate antibacterial and good antifungal activities.
Asunto(s)
Antiprotozoarios/farmacología , Organismos Acuáticos/efectos de los fármacos , Bacillus/química , Lipopéptidos/farmacología , Péptidos Cíclicos/farmacología , Phytophthora/efectos de los fármacos , Antiprotozoarios/química , Antiprotozoarios/aislamiento & purificación , Movimiento Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Lipopéptidos/química , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Péptidos Cíclicos/químicaRESUMEN
Crispene E, a new clerodane-type diterpene, inhibited STAT3 dimerization in a cell-free fluorescent polarisation assay and was found to have significant toxicity against STAT3-dependent MDA-MB 231 breast cancer cell line and selectively inhibited the expression of STAT3 and STAT3 target genes cyclin D1, Fascin and bcl-2. Molecular docking studies suggest the molecule inhibits STAT3 by interacting with its SH2 domain. The compound has been isolated from Tinospora crispa and characterized using standard spectroscopic techniques.
Asunto(s)
Neoplasias de la Mama/patología , Diterpenos de Tipo Clerodano/farmacología , Multimerización de Proteína/efectos de los fármacos , Factor de Transcripción STAT3/química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , Modelos Moleculares , Estructura Cuaternaria de Proteína , Factor de Transcripción STAT3/genéticaRESUMEN
Antifungal resistance and toxicity problems of conventional fungicides highlighted the requirement of search for new safe antifungal agents. To comply with the requirement, we discovered four new non-cytotoxic lipopeptides, gageopeptides A-D, 1-4, from a marine-derived bacterium Bacillus subtilis. The structures and stereochemistry of gageopeptides were determined by NMR data analysis and chemical means. Gageopeptides exhibited significant antifungal activities against pathogenic fungi Rhizoctonia solani, Botrytis cinerea, and Colletotrichum acutatum with minimum inhibitory concentration (MIC) values of 0.02-0.06 µM. In addition, these lipopeptides showed significant motility inhibition and lytic activities against zoospores of the late blight pathogen Phytophthora capsici. These compounds also showed potent antimicrobial activity against Gram positive and Gram negative bacteria with MIC values of 0.04-0.08 µM. However, gageopeptides A-D did not exhibit any cytotoxicity (GI50 > 25 µM) against cancer cell lines in sulforhodamine B (SRB), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), and WST-1 ((4-[3-4-iodophenyl]-2-(4-nitrophenyl)-2H-5-tetrazolio)-1,3-benzene disulfonate)) assays, demonstrating that these compounds could be promising candidates for the development of non-cytotoxic antifungal agents.
Asunto(s)
Antifúngicos/química , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Bacillus subtilis/química , Antibacterianos/farmacología , Botrytis/efectos de los fármacos , Línea Celular Tumoral , Colletotrichum/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Humanos , Lipopéptidos/química , Lipopéptidos/aislamiento & purificación , Lipopéptidos/farmacología , Pruebas de Sensibilidad Microbiana , Phytophthora/efectos de los fármacos , Rhizoctonia/efectos de los fármacos , Rodaminas/químicaRESUMEN
Gageotetrins A-C (1-3), a unique class of linear lipopeptides, consisting of di- and tetrapeptides and a new fatty acid were isolated from a Marine Bacillus subtilis. The structures of 1-3 were assigned by spectroscopic data and their absolute stereochemistries were ascertained by chemical derivatization. Compounds 1-3 displayed good antimicrobial activities with MIC values of 0.01-0.06 µM. However, these compounds failed to register any cytotoxicity (GI50 > 30 µg/ml) against human cancer cell lines.
Asunto(s)
Antiinfecciosos/química , Antiinfecciosos/farmacología , Bacillus subtilis/química , Ácidos Grasos/química , Lipopéptidos/química , Antiinfecciosos/aislamiento & purificación , Bacillus subtilis/aislamiento & purificación , Bacillus subtilis/metabolismo , Línea Celular Tumoral , Ensayos de Selección de Medicamentos Antitumorales , Ácidos Grasos/metabolismo , Fermentación , Humanos , Lipopéptidos/aislamiento & purificación , Lipopéptidos/farmacología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Resonancia Magnética Nuclear BiomolecularRESUMEN
BACKGROUND: Ravenia spectabilis is a medium tall shrub found widespread in South America. It also found in India, Pakistan, Bangladesh etc. Few alkaloid and steroid compounds were reported from the plant previously. MATERIALS AND METHODS: Methanol extract from the stems of Ravenia spectabilis were partitioned into n-hexane, carbon tetrachloride, chloroform and aqueous soluble fractions, respectively. The crude methanol extract, carbon tetrachloride fraction and chloroform fraction were fractionated by column chromatography of Silica gel and Sephadex LH-20 for isolation and purification of compounds. The structures of the isolated compounds were determined by extensive NMR spectral analysis, including 2D NMR, mass spectroscopy etc. RESULTS: Ten compounds, γ-fagarine (1), ravenoline (2), N-methyl atanine (3),2,3,3,5-tetramethyl-2,3,4,5- tetrahydrofurano [3,2-c] quinolin-4-one (4), arborinine (5), 3-geranyl indole (6), atanine (7), steroids sitosta-4-en- 3-one (8), stigmasterol (9) and 3-methoxy-4-hydroxy cinnamic acid (10) were isolated from the stems of Ravenia spectabilis. CONCLUSION: Compounds N-methyl atanine (3), 2,3,3,5-tetramethyl-2,3,4,5-tetrahydrofurano [3,2-c] quinolin-4-one (4), 3-geranyl indole (6), sitosta-4-en-3-one (8) and 3-methoxy-4-hydroxy cinnamic acid (10) were isolated from this plant for the first time. 3-geranyl indole (6) was also isolated second time from natural sources.
RESUMEN
Cristatin A (1a/b), a prenylated dioxopiperazine alkaloid, has been shown to bind selectively to telomeric quadruplex DNA using a FRET-based DNA melting assay. Crucially, the molecule is more drug-like than most previously identified quadruplex-binding agents, and provides a unique chemical scaffold for future chemical biology and drug discovery studies.
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Acacia/química , Alcaloides/química , Antineoplásicos/química , G-Cuádruplex , Telómero/química , Triptófano/análogos & derivados , Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Sitios de Unión , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , ADN/química , Descubrimiento de Drogas , Transferencia Resonante de Energía de Fluorescencia , Humanos , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Desnaturalización de Ácido Nucleico , Hojas de la Planta/química , Triptófano/química , Triptófano/aislamiento & purificación , Triptófano/farmacologíaRESUMEN
The hydroethanolic extract of the flowering tops of Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, a Bangladeshi medicinal plant, was studied for its potential hypoglycemic effect and antioxidant property in alloxan-induced diabetic rats. The extract induced significant reduction in serum glucose, and transaminases, e.g. aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatases (ALP), activities. Significant changes in the thiobarbituric acid reactive substances (TBARS), peroxidase and catalase levels during the experimental period were also observed. The results established that the hydroethanolic extract of the flowering tops of A. cadamba possesses hypoglycemic property and is able to protect liver and brain from oxidative damages caused by diabetes.
RESUMEN
The stem bark of Polyalthia simiarum has yielded a new bisnor-type clerodane diterpenoid, 2-oxo-14,15-bisnor-3,11E-kolavadien-13-one (1), and three previously known clerodane derivatives, kolavenic acid (2), 16beta-hydroxycleroda-3,13(14)Z-dien-15,16-olide (3), and 16-oxocleroda-3,13(14)E-dien-15-oic acid (4). The structures of these compounds were unambiguously determined by extensive NMR studies as well as by comparison with related compounds. Till now this is the second report of the occurrence of any unusual C-18 clerodane diterpenoid from nature. The crude light petroleum extract and the purified compound 3 demonstrated moderate free radical scavenging activity with IC50 values of 21.5 and 23.5 microg/mL, respectively.
Asunto(s)
Diterpenos de Tipo Clerodano/aislamiento & purificación , Polyalthia/química , Diterpenos/aislamiento & purificación , Diterpenos de Tipo Clerodano/químicaRESUMEN
Trans-isoferulic acid was isolated from the carbon tetrachloride soluble fraction of a methanol extract of the rhizomes of Curcuma longa (Zingiberaceae). The structure of the isolated compound was elucidated by comprehensive analysis of spectroscopic data. This is the first report of its occurrence from this plant.
El ácido trans-isoferulico fue aislado de la fracción soluble en tetracloruro de carbono del extracto metanólico de los rizomas de Curcuma Longa (Zingeraceae). La estructura del compuesto aislado fue elucidada por análisis de los datos espectroscópicos. Este es el primer reporte de su presencia en esta planta.
Asunto(s)
Cinamatos/aislamiento & purificación , Curcuma/química , Cinamatos/análisis , Análisis EspectralRESUMEN
Six compounds, namely, Lupeol (1), Betulin (2), beta-Taraxerol (3), Taraxerone (4), Stigmasterol (5) and beta-Sitosterol (6) were isolated from the petroleum ether extract of the leaves of Sapium baccatum based on spectroscopic evidence. Lupeol (1), Betulin (2) and Stigmasterol (5) were isolated for the first time from this plant. The cytotoxic potential of the different solvent extracts (methanol, petroleum ether, carbon-tetrachloride and dichloromethane); six column fractions (F-4, F-7, F-10, F-12, F-18 and F-22) of petroleum ether extract and three pure compounds 1, 4 and 6 were determined by using brine shrimp lethality bioassay. The LC50 of all the tested samples were showed to be lethal to brine shrimp nauplii. However, petroleum ether, carbon-tetrachloride extract, column fractions F-4 and F-18 of petroleum ether extract and pure compound 6 showed quite potent activity in brine shrimp lethality bioassay with LC50 1.33, 1.35, 1.40, 1.58 and 1.58 microg/ml, respectively. These result suggested that they might be contain antitumor or pesticidal activity. Further, the methanol extract and four column fractions (F-7, F-12, F-18 and F-22) of petroleum ether showed significant activity against the tested microorganisms.
Asunto(s)
Antibacterianos/farmacología , Citotoxinas/farmacología , Extractos Vegetales/farmacología , Sapium/química , Animales , Artemia/efectos de los fármacos , Fraccionamiento Químico , Longevidad/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Hojas de la Planta/química , Solventes/química , Pruebas de Toxicidad AgudaRESUMEN
Three macrocyclic diarylheptanoids, 6'-hydroxygaruganin V (1), 9'-desmethylgarugamblin I (2) and 1,9'-didesmethylgaruganin III (3) were isolated from the petroleum ether and dichloromethane extracts of the stem bark of Garuga pinnata. The structures of these compounds were established by extensive spectroscopic studies, including high field NMR and MS measurements.
Asunto(s)
Burseraceae/química , Diarilheptanoides/química , Diarilheptanoides/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Corteza de la Planta/química , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Plantas Medicinales/químicaRESUMEN
Two quinones, stereochenols A (1) and B (2) were isolated from a methanol extract of the stem bark of Stereospermum chelonoides, in addition to the known naphthoquinones, sterekunthal B (3) and sterequinone C (4). The structures of these compounds were established by extensive spectroscopic analyses and by comparison of their spectral data with those of related compounds.
Asunto(s)
Bignoniaceae/química , Quinonas/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Corteza de la Planta/química , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Quinonas/aislamiento & purificaciónAsunto(s)
Meliaceae/química , Sesquiterpenos de Guayano/análisis , Alcanos , Bangladesh , Espectroscopía de Resonancia Magnética/métodos , Medicina Tradicional , Meliaceae/clasificación , Corteza de la Planta/química , Extractos Vegetales/química , Tallos de la Planta/química , Sesquiterpenos de Guayano/aislamiento & purificaciónRESUMEN
As part of the phytochemical studies of the plant genus Glycosmis, the constituents of the plant Glycosmis arborea were investigated. Three new carbazole alkaloids named glybomines A (1), B (2), and C (3), along with known monomeric alkaloids belonging to the carbazole, quinazoline, furoquinoline, quinolone, and acridone classes, were isolated from stems of the plant collected at Mymensing in Dhaka, Bangladesh. Glybomine A (1) is the first example of a 2,5-oxygenated carbazole alkaloid from natural sources. As a primary screening test for anti-tumor promoters, nine alkaloids isolated from this plant have been tested for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) induction by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). All alkaloids tested showed inhibitory activity.
Asunto(s)
Alcaloides/aislamiento & purificación , Antígenos Virales/efectos de los fármacos , Carbazoles/aislamiento & purificación , Plantas Medicinales/química , Rutaceae/química , Alcaloides/química , Alcaloides/farmacología , Bangladesh , Carbazoles/química , Carbazoles/farmacología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Two known steroids, stigmasterol and 6b-hydroxystigmasta-4,22-dien-3-one (1) and two unusual polyketides, cis-4-hydroxymellein (2) and trans-4-hydroxymellein (3) were isolated from the stem bark of Uvaria hamiltonii. The structures of the compounds were elucidated independently by high-resolution 2D-NMR techniques and confirmed by comparison with previously reported values.