Do betaine lipids replace phosphatidylcholine as fatty acid editing hubs in microalgae?

Acyl editing refers to a deacylation and reacylation cycle on a lipid, which allows for fatty acid desaturation and modification prior to being removed and incorporated into other pools. Acyl editing is an important determinant of glycerolipid synthesis and has been well-characterized in land plants, thus this review begins with an overview of acyl editing in plants. Much less is known about acyl editing in algae, including the extent to which acyl editing impacts lipid synthesis and on which lipid substrate(s) it occurs. This review compares what is known about acyl editing on its major hub phosphatidylcholine (PC) in land plants with the evidence for acyl editing of betaine lipids such as diacylglyceryltrimethylhomoserine (DGTS), the structural analog that replaces PC in several species of microalgae. In land plants, PC is also known to be a major source of fatty acids and diacylglycerol (DAG) for synthesis of the neutral lipid triacylglycerol (TAG). We review the evidence that DGTS contributes substantially to TAG accumulation in algae as a source of fatty acids, but not as a precursor to DAG. We conclude with evidence of acyl editing on other membrane lipid substrates in plants and algae apart from PC or DGTS, and discuss future analyses to elucidate the role of DGTS and other betaine lipids in acyl editing in microalgae.

Implicit and Explicit Number-Space Associations Differentially Relate to Interference Control in Young Adults With ADHD.

Behavioral evidence for the link between numerical and spatial representations comes from the spatial-numerical association of response codes (SNARC) effect, consisting in faster reaction times to small/large numbers with the left/right hand respectively. The SNARC effect is, however, characterized by considerable intra- and inter-individual variability. It depends not only on the explicit or implicit nature of the numerical task, but also relates to interference control. To determine whether the prevalence of the latter relation in the elderly could be ascribed to younger individuals' ceiling performances on executive control tasks, we determined whether the SNARC effect related to Stroop and/or Flanker effects in 26 young adults with ADHD. We observed a divergent pattern of correlation depending on the type of numerical task used to assess the SNARC effect and the type of interference control measure involved in number-space associations. Namely, stronger number-space associations during parity judgments involving implicit magnitude processing related to weaker interference control in the Stroop but not Flanker task. Conversely, stronger number-space associations during explicit magnitude classifications tended to be associated with better interference control in the Flanker but not Stroop paradigm. The association of stronger parity and magnitude SNARC effects with weaker and better interference control respectively indicates that different mechanisms underlie these relations. Activation of the magnitude-associated spatial code is irrelevant and potentially interferes with parity judgments, but in contrast assists explicit magnitude classifications. Altogether, the present study confirms the contribution of interference control to number-space associations also in young adults. It suggests that magnitude-associated spatial codes in implicit and explicit tasks are monitored by different interference control mechanisms, thereby explaining task-related intra-individual differences in number-space associations.

Mathematical abilities in elementary school: Do they relate to number-space associations?

Considering the importance of mathematics in Western societies, it is crucial to understand the cognitive processes involved in the acquisition of more complex mathematical skills. The current study, therefore, investigated how the quality of number-space mappings on the mental number line, as indexed by the parity SNARC (spatial-numerical association of response codes) effect, relates to mathematical performances in third- and fourth-grade elementary school children. Mathematical competencies were determined using the "Heidelberger Rechentest," a standardized German math test assessing both arithmetical and visuospatial math components. Stronger parity SNARC effects significantly related to better arithmetical but not visuospatial math abilities, albeit only in the relatively younger children. These findings highlight the importance of spatial-numerical interactions for arithmetical (as opposed to visuospatial) math skills at the fairly early stages of mathematical development. Differential relations might be explained by the reliance on problem-solving strategies involving number-space mappings only for arithmetic tasks mainly in younger children.

How Math Anxiety Relates to Number-Space Associations.

Given the considerable prevalence of math anxiety, it is important to identify the factors contributing to it in order to improve mathematical learning. Research on math anxiety typically focusses on the effects of more complex arithmetic skills. Recent evidence, however, suggests that deficits in basic numerical processing and spatial skills also constitute potential risk factors of math anxiety. Given these observations, we determined whether math anxiety also depends on the quality of spatial-numerical associations. Behavioral evidence for a tight link between numerical and spatial representations is given by the SNARC (spatial-numerical association of response codes) effect, characterized by faster left-/right-sided responses for small/large digits respectively in binary classification tasks. We compared the strength of the SNARC effect between high and low math anxious individuals using the classical parity judgment task in addition to evaluating their spatial skills, arithmetic performance, working memory and inhibitory control. Greater math anxiety was significantly associated with stronger spatio-numerical interactions. This finding adds to the recent evidence supporting a link between math anxiety and basic numerical abilities and strengthens the idea that certain characteristics of low-level number processing such as stronger number-space associations constitute a potential risk factor of math anxiety.

Inhibition of return and attentional facilitation: Numbers can be counted in, letters tell a different story.

Prior research has provided strong evidence for spatial-numerical associations. Single digits can for instance act as attentional cues, orienting visuo-spatial attention to the left or right hemifield depending on the digit's magnitude, thus facilitating target detection in the cued hemifield (left/right hemifield after small/large digits, respectively). Studies using other types of behaviourally or biologically relevant central cues known to elicit automated symbolic attention orienting effects such as arrows or gaze have shown that the initial facilitation of cued target detection can turn into inhibition at longer stimulus onset asynchronies (SOAs). However, no studies so far investigated whether inhibition of return (IOR) is also observed using digits as uninformative central cues. To address this issue we designed an attentional cueing paradigm using SOAs ranging from 500 ms to 1650 ms. As expected, the results showed a facilitation effect at the relatively short 650 ms SOA, replicating previous findings. At the long 1650 ms SOA, however, participants were faster to detect targets in the uncued hemifield compared to the cued hemifield, showing an IOR effect. A control experiment with letters showed no such congruency effects at any SOA. These findings provide the first evidence that digits not only produce facilitation effects at shorter intervals, but also induce inhibitory effects at longer intervals, confirming that Arabic digits engage automated symbolic orienting of attention.

Task instructions determine the visuospatial and verbal-spatial nature of number-space associations.

Evidence for number-space associations comes from the spatial-numerical association of response codes (SNARC) effect, consisting in faster reaction times to small/large digits with the left/right hand, respectively. Two different proposals are commonly discussed concerning the cognitive origin of the SNARC effect: the visuospatial account and the verbal-spatial account. Recent studies have provided evidence for the relative dominance of verbal-spatial over visuospatial coding mechanisms, when both mechanisms were directly contrasted in a magnitude comparison task. However, in these studies, participants were potentially biased towards verbal-spatial number processing by task instructions based on verbal-spatial labels. To overcome this confound and to investigate whether verbal-spatial coding mechanisms are predominantly activated irrespective of task instructions, we completed the previously used paradigm by adding a spatial instruction condition. In line with earlier findings, we could confirm the predominance of verbal-spatial number coding under verbal task instructions. However, in the spatial instruction condition, both verbal-spatial and visuospatial mechanisms were activated to an equal extent. Hence, these findings clearly indicate that the cognitive origin of number-space associations does not always predominantly rely on verbal-spatial processing mechanisms, but that the spatial code associated with numbers is context dependent.

The impact of inhibition capacities and age on number-space associations.

Numerical and spatial representations are tightly linked, i.e., when doing a binary classification judgment on Arabic digits, participants are faster to respond with their left/right hand to small/large numbers, respectively (Spatial-Numerical Association of Response Codes, SNARC effect, Dehaene et al. in J Exp Psychol Gen 122:371-396, 1993). To understand the underlying mechanisms of the well-established SNARC effect, it seems essential to explore the considerable inter-individual variability characterizing it. The present study assesses the respective roles of inhibition, age, working memory (WM) and response speed. Whereas these non-numerical factors have been proposed as potentially important factors to explain individual differences in SNARC effects, none (except response speed) has so far been explored directly. Confirming our hypotheses, the results show that the SNARC effect was stronger in participants that had weaker inhibition abilities (as assessed by the Stroop task), were relatively older and had longer response times. Interestingly, whereas a significant part of the age influence was mediated by cognitive inhibition, age also directly impacted the SNARC effect. Similarly, cognitive inhibition abilities explained inter-individual variability in number-space associations over and above the factors age, WM capacity and response speed. Taken together our results provide new insights into the nature of number-space associations by describing how these are influenced by the non-numerical factors age and inhibition.

The impact of mathematical proficiency on the number-space association.

A specific instance of the association between numerical and spatial representations is the SNARC (Spatial Numerical Association of Response Codes) effect. The SNARC effect describes the finding that during binary classification of numbers participants are faster to respond to small/large numbers with the left/right hand respectively. Even though it has been frequently replicated, important inter-individual variability has also been reported. Mathematical proficiency is an obvious candidate source for inter-individual variability in numerical judgments, but studies investigating its influence on the SNARC effect remain scarce. The present experiment included a total of 95 University students, divided into three groups differing significantly in their mathematical proficiency levels. Using group analyses, it appeared that the three groups differed significantly in the strength of their number-space associations in a parity judgment task. This result was further confirmed on an individual level, with higher levels in arithmetic leading to relatively weaker SNARC effects. To explain this negative relationship we propose accounts based on differences in access to qualitatively different numerical representations and also consider more domain general factors, with a focus on inhibition capacities.

Developing number-space associations: SNARC effects using a color discrimination task in 5-year-olds.

Human adults' numerical representation is spatially oriented; consequently, participants are faster to respond to small/large numerals with their left/right hand, respectively, when doing a binary classification judgment on numbers, known as the SNARC (spatial-numerical association of response codes) effect. Studies on the emergence and development of the SNARC effect remain scarce. The current study introduces an innovative new paradigm based on a simple color judgment of Arabic digits. Using this task, we found a SNARC effect in children as young as 5.5 years. In contrast, when preschool children needed to perform a magnitude judgment task necessitating exact number knowledge, the SNARC effect started to emerge only at 5.8 years. Moreover, the emergence of a magnitude SNARC but not a color SNARC was linked to proficiency with Arabic digits. Our results suggest that access to a spatially oriented approximate magnitude representation from symbolic digits emerges early in ontogenetic development. Exact magnitude judgments, on the other hand, rely on experience with Arabic digits and, thus, necessitate formal or informal schooling to give access to a spatially oriented numerical representation.

The epidemiology of mild cognitive impairment (MCI) and Alzheimer's disease (AD) in community-living seniors: protocol of the MemoVie cohort study, Luxembourg.

BACKGROUND: Cognitive impairment and Alzheimer's disease (AD) are increasingly considered a major public health problem. The MemoVie cohort study aims to investigate the living conditions or risk factors under which the normal cognitive capacities of the senior population in Luxembourg (≥ 65 year-old) evolve (1) to mild cognitive impairment (MCI) - transitory non-clinical stage - and (2) to AD. Identifying MCI and AD predictors undeniably constitutes a challenge in public health in that it would allow interventions which could protect or delay the occurrence of cognitive disorders in elderly people. In addition, the MemoVie study sets out to generate hitherto unavailable data, and a comprehensive view of the elderly population in the country. METHODS/DESIGN: The study has been designed with a view to highlighting the prevalence in Luxembourg of MCI and AD in the first step of the survey, conducted among participants selected from a random sample of the general population. A prospective cohort is consequently set up in the second step, and appropriate follow-up of the non-demented participants allows improving the knowledge of the preclinical stage of MCI. Case-control designs are used for cross-sectional or retrospective comparisons between outcomes and biological or clinical factors. To ensure maximal reliability of the information collected, we decided to opt for structured face to face interviews. Besides health status, medical and family history, demographic and socio-cultural information are explored, as well as education, habitat network, social behavior, leisure and physical activities. As multilingualism is expected to challenge the cognitive alterations associated with pathological ageing, it is additionally investigated. Data relative to motor function, including balance, walk, limits of stability, history of falls and accidents are further detailed. Finally, biological examinations, including ApoE genetic polymorphism are carried out. In addition to standard blood parameters, the lipid status of the participants is subsequently determined from the fatty acid profiles in their red blood cells. The study obtained the legal and ethical authorizations. DISCUSSION: By means of the multidisciplinary MemoVie study, new insights into the onset of cognitive impairment during aging should be put forward, much to the benefit of intervention strategies as a whole.

Oxytocin does not contribute to the effects of cervical dilation on progesterone secretion and embryonic development in mares.

The aim of the present study was, to investigate the effects of oxytocin administration on Day 7 post-ovulation on progesterone secretion, pregnancy rate and embryonic growth in mares. Endogenous stimulation of oxytocin release was compared to the administration of native oxytocin or the long-acting oxytocin analogue carbetocin. At Day 7 after ovulation, mares had to undergo four treatments in a crossover design: (a) control, (b) oxytocin (10 IU i.v.), (c) carbetocin (280 microg i.m.) and (d) cervical dilation. On Day 13, all mares (8 of 8 mares) were pregnant on groups control, oxytocin and carbetocin and only 6 of 8 mares on group dilation. In one mare uterine fluid accumulation and uterine edema from Day 6 to 13 and early embryonic death by Day 11 occurred during dilation treatment. Another mare, which did not become pregnant during dilation treatment, developed uterine fluid accumulation and uterine edema from Day 10 to 14. Mean growth rates of the conceptuses did not differ among treatment groups and individual growth rates varied in a wide range from -0.1 to 0.8 cm per day. At Day 13, mean diameters of conceptuses yielded 1.4+/-0.1 cm in control group, 1.5+/-0.1 in oxytocin and carbetocin group and 1.3+/-0.2 cm in dilation group. Secretion of progesterone was not affected by treatments. Administration of oxytocin and carbetocin caused similar maximum plasma concentrations of oxytocin, but onset and duration of peaks differed. Maximum concentrations after intramuscular application of carbetocin were obtained almost 20 min later when compared to intravenous administration of oxytocin. Duration of peaks after injection of the long-acting oxytocin analogue was more than three-fold longer than after administration of native oxytocin. In conclusion, the present study showed that single administration of oxytocin or its long-acting analogue carbetocin at Day 7 after ovulation did not affect progesterone secretion, pregnancy rate and embryonic growth. Two possible scenarios concerning the effects of cervical dilation were observed: In the majority of mares, dilation of the caudal half to two-third of the cervical lumen up to a diameter of 4.5 cm had no negative consequences on progesterone secretion and pregnancy outcome. However, cervical dilation caused uterine inflammation and subsequent luteolysis in two mares and early embryonic death in one of them. Thus, manipulation of the cervix itself seems not to have negative impact on success rates of transcervical transfer of embryos in the mare.