Sporadic paediatric and adult Burkitt lymphomas share similar phenotypic and genotypic features.

AIMS: To characterize the clinicopathological features of sporadic Burkitt lymphoma (BL). METHODS AND RESULTS: A retrospective study of 17 paediatric and 14 adult BLs with history and histopathology review, immunohistochemistry, Epstein-Barr virus (EBV) in situ hybridization (EBER) and fluorescence in situ hybridization. There was no statistically significant difference in gender, frequency of central nervous system (CNS) involvement and leukaemic change at presentation, or frequency of CD10+/Bcl-2-/Bcl-6+ (88% versus 86%), Ki67 labelling index, EBER (24% versus 21%), or C-MYC translocation (100% versus 92%) between paediatric and adult tumours. Correct pretreatment diagnoses were made in 13/17 (76%) paediatric and in 9/14 (64%) adult tumours. Twenty-eight patients received chemotherapy including 13/16 (81%) paediatric and 3/12 (25%) adult patients with appropriate regimens; 16 (57%) received CNS prophylaxis. The 1- and 5-year overall survival (OS) rates for paediatric patients were 80% and 50%, respectively, whereas 1-year OS for adults was 15%. CONCLUSIONS: Sporadic paediatric and adult BLs were phenotypically and genotypically similar. The significant prognosticators were age (P = 0.001), with or without CNS prophylaxis (P = 0.004), and CNS involvement (P = 0.008) and leukaemic change (P = 0.019) in disease course. The poor outcome in adult patients might be related to incorrect diagnosis and inappropriate treatment.

New techniques on deformed image motion estimation and compensation.

In this paper, new techniques for deformed image motion estimation and compensation using variable-size block-matching are proposed, which can be applied to an image sequence compression system or a moving object recognition system. The motion estimation and compensation techniques have been successfully applied in the area of image sequence coding. Many research papers on improving the performance of these techniques have been published; many directions are proposed, which can all lead to better performance than the conventional techniques. Among them, both generalized block-matching and variable-size block-matching are successfully applied in reducing the data rate of compensation error and motion information, respectively. These two algorithms have their merits, but suffer from their drawbacks. Moreover, reducing the data rate in compensation error is sometimes increasing the data rate in motion information, or vice versa. Based on these two algorithms, we propose and examine several algorithms which are effective in reducing the data rate. We then incorporate these algorithms into a system, in which they work together to overcome the disadvantages to individual and keep their merits at the same time. The proposed system can optimally balance the amount of data rate in two aspects (i.e., compensation error and motion information). Experimental results show that the proposed system outweighs the conventional techniques. Since we propose a recovery operation which tries to recover the incorrect motion vectors from the global motion, this proposed system can also be applied to the moving object recognition in image sequences.

Renal endosomal phosphate (Pi) transport in normal and diabetic rats and response to chronic Pi deprivation.

Chronic renal adaptation to dietary deprivation of Pi is accompanied by increased Na+/Pi co-transport across the brush border membrane of the renal proximal tubule. The increased activity of this co-transport system depends on de novo protein synthesis and insulin. The present study used normal and diabetic rats to determine if the endosomal pool of Na+/Pi co-transporters was altered by Pi deprivation and the possible role of insulin. In response to 5 days of dietary Pi deprivation there was a significant increase in endosomal Na+/Pi co-transport in control rats but there was no change in diabetic rats. The increase in endosomal Pi uptake was restored in diabetic rats treated with exogenous insulin. Na(+)-independent Pi uptake and proline uptake remained unchanged in all groups. The changes in endosomal Na+/Pi co-transport correlated with the abundance of the specific Na+/Pi co-transporter protein, as determined by Western blots. The pattern of endosomal changes paralleled that observed in brush border membranes. One possibility consistent with these findings is that the endosomal fraction contains newly synthesized Na+/Pi co-transporters targeted for delivery to the apical brush border membrane. Increased synthesis and delivery is required to maintain the adaptation to chronic Pi deprivation.

Characterization and nucleotide sequences of the variable regions of a monoclonal antibody against alpha-fetoprotein.

alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy.

Application of industrial protease "Alcalase" in peptide synthesis.

An industrial alkaline protease "Alcalase" has been found to be very stable in organic solvents and usable as a catalyst for i) resolution of N-protected amino acids, in both aqueous solution and organic solvent with high yield and optical purity, ii) saponification of Fmoc-peptide esters in high concentration organic solvent iii) kinetically controlled peptide bond formation in ethanol solution, iv) diastereoselective hydrolysis of peptide esters, to prepare optically pure peptides.