Influence of substrate/inoculum ratio, inoculum source and ammonia inhibition on anaerobic digestion of poultry waste.

Poultry wastes are rich in organic matter, allowing their use as substrates for biogas production by anaerobic digestion (AD). The major difficulty in the anaerobic digestion of this protein-rich waste is ammonia inhibition. Different results of biochemical methane potential (BMP) were obtained after the mesophilic anaerobic digestion of different avian waste in batch mode. It was shown that using two different inoculum (Liger and Saint-Brieuc) sources and different substrate-to-inoculum (S/I) ratios does not have a significant effect on the biochemical methane potential of organic laying hen droppings (OLHD); an average of 0.272 Nm3 CH4·kg-1·VS was obtained with both inocula. Otherwise, it affects the hydrolysis constant KH, and it decreases when the substrate-to-inoculum ratio increases. Furthermore, Liger is the most suitable inoculum for our substrate because it shows stability during the process even with different organic loads. Comparing the biochemical methane potential of multiple avian wastes such as organic laying hen droppings and different slaughterhouse waste highlights the importance of slaughterhouse waste in the anaerobic digestion process because of the high methane yield observed especially with the viscera (0.779 Nm3 CH4·kg-1 VS, SD = 0.027 Nm3 CH4·kg-1 VS). Moreover, methane production was affected by increasing the ammonia concentrations; when [N-NH3] > 9.8 g·N-NH3·L-1, the biochemical methane potential decreases and the lag phase increases (λ > 30 days); a total inhibition of the process was observed when ammonia concentration is above 21.8 g·L-1.

Anaerobic digestion of waste Tunisian date (Phoenix dactylifera L.): effect of biochemical composition of pulp and seeds from six varieties.

Large amounts of secondary date waste (pulp and seeds) are produced and discarded in Tunisia without proper valorisation methods. To study the possibility of valorising different varieties of Tunisian date waste (pulp and seeds) via anaerobic digestion, batch biochemical methane potential (BMP) tests were carried out under mesophilic temperature. The bio-methane production curves were fitted to the model of modified Gompertz in search of the kinetic parameters. The bio-chemical characterisation of the substrates from different varieties (total and volatile solids, COD and contents in carbohydrates, proteins, lipids, polyphenols) was realised. Principal component analysis (PCA) was used to investigate the correlations between the model parameters and biochemical variables. Results show that the biochemical compositions of date pulp and seeds strongly depend on the varieties. The BMP are in the range of 0.295-0.345 and 0.267-0.327 Nm3 CH4·kg COD-1 for pulp and seeds respectively, resulting from the significant biochemical variance among the varieties. The BMP of date seeds was significantly correlated with their VS/TS ratio, carbohydrate and protein contents (p < 0.05). For the pulp, significant correlation was found between BMP, carbohydrate and lipid contents. PCA shows that certain varieties (like pulp and seeds of Deglet Nour and seeds of Bejou) are most suitable for being valorised by anaerobic digestion. The most suitable date varieties for this innovative approach were revealed. This research provided useful knowledge for bioconversion of waste date pulp and seeds to biomass energy.

Hygienization of mixed animal by-product using pulsed electric field in a continuous treatment system: Synergistic effect with ohmic heating on the inactivation of indicator bacteria.

Thermal hygienization of waste animal by-products (ABP) before anaerobic digestion is imposed by EU regulations in order to minimize its sanitary risk during digestate land application. This process is energy and time consuming. The present paper deals with the hygienization of ABP using pulsed electric field (PEF) in a continuous system. Enterococcus faecalis ATCC 19433 and Escherichia coli ATCC 25922 were tested as indicator bacteria characterizing the microbial inactivation efficiency. Four electric field strengths (15, 20, 25 and 30 kV∙cm-1) were applied to the continuous treatment chamber where circulated the ABP suspension. Synergistic effect of PEF and ohmic heating (Tave = 41 °C) and single effect of PEF (Tave = 28 °C) on bacterial inactivation were investigated. With the effect of ohmic heating, PEF treatment at 25 and 30 kV∙cm-1 for 0.9 ms could obtain 5-log10 reduction of Ent. faecalis. This efficiency complies with the EU criteria to validate an alternative hygienization process. The time estimated by Weibull model for 5-log10 reduction of both indicator bacteria (5-D value) was significantly reduced by 2-24.5 times when the synergistic effect of PEF and ohmic heating was present. The increase in electric field strength from 25 kV∙cm-1 to 30 kV∙cm-1 did not amount to a further inactivation. PEF process coupling ohmic heating at 25 kV∙cm-1 was the most efficient for ABP hygienization among the four electric field strengths studied.

Influence of pretreatment conditions on lignocellulosic fractions and methane production from grape pomace.

The lignocellulosic structure of grape pomace requires the use of pretreatments facilitating microbial decomposition of the matter and enhancing methane production. In this study, the effects of various pretreatments (freezing, alkaline treatment using NaOH and NH3, acid treatment using HCl, ultrasounds and pulsed electric fields) were examined in batch mode. The highest methane production (0.178Nm3kg-1 of COD) was attained after alkaline treatment with 10% NaOH w/w dry basis, at 20°C and for 24h. This result is due to the degradation of more than 50% of lignin and about 22% of cellulose present in grape pomace. The coupling of this pretreatment with freezing at -20°C exhibited the highest methane production of 0.2194±0.0007Nm3kg-1 of COD. When applied to a larger scale continuous digester, this coupled pretreatment increased methane production by about 27%, compared to the untreated samples, promoting the green valorization of the biomass.

Metronidazole removal by means of a combined system coupling an electro-Fenton process and a conventional biological treatment: By-products monitoring and performance enhancement.

In order to mineralize Metronidazole (MTZ), a process coupling an electro-Fenton pretreatment and a biological degradation was implemented. A mono-compartment batch reactor containing a carbon-felt cathode and a platinum anode was employed to carry out the electro-Fenton pretreatment of MTZ. A total degradation of MTZ (100 mg L-1) was observed at 0.07 mA.cm-2 after only 20 min of electrolysis. Yet, after 1 and 2 h of electrolysis, the mineralization level remained low (16.2% and 32% respectively), guaranteeing a significant residual organic content for further biological treatment. LCMS/MS was used to determine the intermediates by-products and hence to propose a plausible degradation pathway. An increase from 0 to 0.44 and 0.6 for 1 and 2 h of electrolysis was observed for the BOD5/COD ratio. Thus, from 1 h of electro-Fenton pretreatment, the electrolysis by-products were considered biodegradable. A biological treatment of the electrolysis by-products after 1 and 2 h was then realized. The mineralization yields reached very close values, about 84% for 1 and 2 h of electrolysis after 504 h of biological treatment, namely close to 89% for the overall process, showing the pertinence of the proposed coupled process.

Reactive oxygen and iron species monitoring to investigate the electro-Fenton performances. Impact of the electrochemical process on the biodegradability of metronidazole and its by-products.

In this study, the monitoring of reactive oxygen species and the regeneration of the ferrous ions catalyst were performed during electro-Fenton (EF) process to highlight the influence of operating parameters. The removal of metronidazole (MTZ) was implemented in an electrochemical mono-compartment batch reactor under various ranges of current densities, initial MTZ and ferrous ions concentrations, and pH values. It was found that under 0.07 mA cm-2, 0.1 mM of ferrous ions and pH = 3, the efficiency of 100 mg L-1 MTZ degradation and mineralization were 100% within 20 min and 40% within 135 min of electrolysis, respectively. The highest hydrogen peroxide and hydroxyl radical concentrations, 1.4 mM and 2.28 mM respectively, were obtained at 60 min electrolysis at 0.07 mA cm-2. Improvement of the biodegradability was reached from 60 min of electrolysis with a BOD5/COD ratio above 0.4, which was reinforced by a respirometric study, that supports the feasibility of coupling electro-Fenton and biological treatment for the metronidazole removal.

Anaerobic digestion of grape pomace: Effect of the hydraulic retention time on process performance and fibers degradability.

To optimize the anaerobic digestion of grape pomace under mesophilic conditions, continuous digesters were operated at different hydraulic retention times (HRT) (30, 20, 15 and 10 days) equivalent to organic loading rates (OLR) of 2.5, 3.7, 5.7 and 7.3 kg COD m-3 d-1, respectively. At HRTs of 30 and 20 days, steady state conditions were observed with methane yields of 0.984 ±â€¯0.013 NL d-1 and 1.362 ±â€¯0.018 NL d-1, respectively. The HRT of 15 days was found critical because of acids accumulation through the experiments. When the OLR of 5.7 kg COD m-3 d-1 was reached, methane production was found to be instable. Finally, at HRT of 10 days, a failure of the system was observed due to the washing of the methanogenic microorganisms. Regarding the degradability of the lignocellulosic fractions, the maximum reduction yields for hemicellulose and cellulose were noted for HRTs of 30 and 20 days, while lignin was not degraded throughout the different experiments. For an optimization of the process, HRT of 20 days can therefore be recommended for productive use in large-scale applications.

Anaerobic digestion of grape pomace: Biochemical characterization of the fractions and methane production in batch and continuous digesters.

In this study, we have estimated the biogas and methane production from grape pomace (variety Cabernet Franc). The physical and chemical characteristics of the raw material were determined, and the structural polysaccharides were identified and analyzed by the Van Soest method. Batch anaerobic digestions were carried out to assess the methane production of the grape pomace, pulp and seeds. The obtained cumulative methane productions are 0.125, 0.165 and 0.052 Nm(3) kg COD(-1) for grape pomace, pulps and seeds, respectively. The effect of grinding on the methane potential of the substrates, as a mechanical pretreatment, was evaluated. We found that it increased the anaerobic biodegradability for grape pomace, pulp and seeds by 13.1%, 4.8% and 22.2%, respectively. On the other hand, the methane potential of the grape pomace was determined in a laboratory pilot plant (12L) continuously mixed with an organic loading rate of 2.5 kg COD m(3) d(-1) and a hydraulic retention time of 30 days. The corresponding biogas production was 6.43 × 10(-3) Nm(3) d(-1), with a methane content of 62.3%. Thus, the pilot plant's efficiency compared to that achieved in the batch process was 81.2%. Finally, a significant correlation was found between the biochemical content and methane production.

Characterization of gaseous odorous emissions from a rendering plant by GC/MS and treatment by biofiltration.

This research focuses on the identification and quantification of odorous components in rendering plant emissions by GC/MS and other analytical methods, as well as the description of phenomena occurring in biofilter in order to improve the removal efficiency of industrial biofilters. Among the 36 compounds quantified in the process air stream, methanethiol, isopentanal and hydrogen sulfide, presented the major odorous contributions according to their high concentrations, generally higher than 10 mg m(-3), and their low odorous detection thresholds. The elimination of such component mixtures by biofiltration (Peat packing material, EBRT: 113 s) was investigated and revealed that more than 83% of hydrogen sulfide and isopentanal were removed by biofilter. Nevertheless, the incomplete degradation of such easily degradable pollutants suggested inappropriate conditions as lack of nutrients and acidic pH. These inadequate conditions could explain the lack of performance, especially observed on methanethiol (53% of RE) and the production of oxygenated and sulfur by-products by the biofilter itself.