Cleaning of long oval canals with WaveOne Gold system associated with different irrigant agitation protocols

Aim: The aim of the study was to evaluate the cleaning of mandibular incisors with WaveOne Gold® (WO) under different preparation techniques. Methods: A total of 210 human mandibular incisors were selected and divided into seven groups (n = 30), prepared by WO single-files (Small 20/.07 ­ WOS; Primary 25/.07 ­ WOP; Medium 35/.06 ­ WOM; or Large 45/.05 - WOL) and sequential-file techniques (WOS to WOP; WOS to WOM; and WOS to WOL). Further subdivision was made according to irrigation protocol: control group (manual irrigation - CON), E1 Irrisonic® - EIR, and EDDY® - EDD. Debris removal and the smear layer were evaluated by scanning electron microscopy. Data were analyzed by using Spearman's correlation test. The significance level was set at 5%. Results: For debris and smear layer removal, WOS and WOP, EIR differed from CON and EDD (p <0.05). Conclusion: Regardless of the instrumentation used, the agitation of the irrigant solution provided better cleanability. These findings reinforce the need for agitation techniques as adjuvants in cleaning root canal systems in mandibular incisors

Influence of activation mode and preheating on intracanal irrigant temperature.

This ex vivo study assessed the effectiveness of two activation modes to increase the intracanal temperature of 5.25% NaOCl solution and the time for a preheated-irrigant to return to its initial temperature. Ten single-rooted extracted human teeth were used. Thermocouples were adapted to the root surface and the temperature was registered following two activation modes: ultrasound (US) and thermal. Furthermore, a preheated-solution (45°C/60°C) was flushed into root canal to test the time needed to return to its initial temperature. Data were analysed using two-way ANOVA and Tukey's post-hoc tests (P = 0.05). Statistical analysis showed differences between the activation modes. US and thermal activation increased from 37 to 40.4°C and to 62.8°C respectively in 60 s. Time for preheated-irrigant to return to its initial temperature was longer for 60°C. Preheating irrigant at 60°C and using thermal activation to keep it warm may represent a further step of the irrigation protocol.

Root Preparation of Deciduous Teeth: Efficacy of WaveOne and ProTaper Systems with and without Passive Ultrasonic Irrigation.

INTRODUCTION: The aim of this study was to compare root preparation of deciduous teeth with WaveOne Large (WO) and ProTaper F4 (PT) instruments with or without passive ultrasonic irrigation (PUI). METHODS AND MATERIALS: Forty-eight deciduous teeth were scanned before and after root preparation and divided in four groups (n=12): WO+EDTA (WO); WO+EDTA with PUI (WOPUI); PTF+EDTA (PT); and PT+EDTA with PUI (PTPUI). Root canal enlargement by micro-computed tomography and root canal cleaning by scanning electron microscopy (SEM) were analyzed. Data were submitted to two-way ANOVA and Tukey's tests to analyze the root canal volume variation, and Kruskal-Wallis followed by Friedman and Wilcoxon tests were used to evaluate the cleaning efficacy. The level of significance was set at 0.05. RESULTS: No significant difference occurred in total volume between groups (P>0.05). On analysis by thirds of the root canal, there was a difference in volume between WO (cervical) compared to WO and PT (apical), and PTPUI (middle and apical) (P<0.05). When cleaning of the thirds within the same group was compared, there was a significant difference in all groups (P<0.05). Among the groups, in the thirds, in the cervical a difference occurred (P=0.028), and the pairwise comparisons indicated statistically difference between WO and PT, and WO and PTPUI (P<0.05). In the pairwise comparisons among thirds, in the groups, difrences occured in all of them when compared the cervical and apical thirds (P<0.05). CONCLUSION: Passive ultrasonic irrigation has not improved the smear layer removal in deciduous teeth. Despite the differences in performance between WO and PT instruments, both were suitable for preparation of deciduous teeth.

Performance of Three Single Instrument Systems in the Preparation of Long Oval Canals.

This study aimed to evaluate the performance of the Wave One, Easy ProDesign Logic and One Shape systems in the preparation of long oval root canals. Forty-two mandibular incisors were randomized into three groups: Group I, Wave One Primary (WO) (#25.08); Group II, Easy ProDesign Logic (EPL) (#25.06) and Group III, One Shape (OS) (#25.06). Micro-computed tomography scans were obtained pre- and post-preparation of the samples. Instrument fractures or loss of working length did not occur in any of the groups. There was a statistically significant difference in total root canal volume between the WO and EPL (p<0.05) and OS systems (p<0.05) The mean percentages of instrumented canal area in the middle thirds were 76.9% in the WO, 62.3% in the EPL and 71.8% in the OS (p>0.05). The OS system had the strongest correlation between volume increase and instrumented area (R=0.63). The WO, EPL and OS systems presented mean preparation times of 2.13 min, 0.54 min, and 2.21 min (p<0.05), respectively. All three systems enabled the safe preparation of long oval root canals. The WO system most effectively increased root canal volume, but it did not affect the instrumented areas. The OS system had the strongest correlation between volume increase and instrumented area, while the EPL system was the fastest.

Performance of Three Single Instrument Systems in the Preparation of Long Oval Canals

Abstract This study aimed to evaluate the performance of the Wave One, Easy ProDesign Logic and One Shape systems in the preparation of long oval root canals. Forty-two mandibular incisors were randomized into three groups: Group I, Wave One Primary (WO) (#25.08); Group II, Easy ProDesign Logic (EPL) (#25.06) and Group III, One Shape (OS) (#25.06). Micro-computed tomography scans were obtained pre- and post-preparation of the samples. Instrument fractures or loss of working length did not occur in any of the groups. There was a statistically significant difference in total root canal volume between the WO and EPL (p<0.05) and OS systems (p<0.05) The mean percentages of instrumented canal area in the middle thirds were 76.9% in the WO, 62.3% in the EPL and 71.8% in the OS (p>0.05). The OS system had the strongest correlation between volume increase and instrumented area (R=0.63). The WO, EPL and OS systems presented mean preparation times of 2.13 min, 0.54 min, and 2.21 min (p<0.05), respectively. All three systems enabled the safe preparation of long oval root canals. The WO system most effectively increased root canal volume, but it did not affect the instrumented areas. The OS system had the strongest correlation between volume increase and instrumented area, while the EPL system was the fastest.

Resumo Este estudo teve como objetivo avaliar o desempenho dos sistemas Wave One, Easy ProDesign Logic e One Shape no preparo de canais radiculares ovais. Quarenta e dois incisivos inferiores foram randomizados em três grupos: Grupo I, Wave One primary (WO) (#25.08); Grupo II, Easy ProDesign Logic (EPL) (#25.06) e Grupo III, One Shape (OS) (#25.06). As amostras foram submetidas a microtomografia computadorizada antes e após o preparo. Não ocorreram fraturas de instrumento ou perda de comprimento de trabalho em qualquer dos três grupos. Houve diferença estatisticamente significante no volume total do canal radicular entre o WO e EPL. A média do percentual de área instrumentada do canal no terço médio foi 76,9% no grupo WO, 62,3% no grupo EPL e 71,8% no grupo OS (p>0,05). O sistema OS teve a correlação mais forte entre o aumento de volume e área instrumentada (R=0,63). Os sistemas WO, EPL e OS apresentaram tempos de preparo médios de 2,13 min, 0,54 min e 2,21 min (p<0,05). Todos os três sistemas foram seguros no preparo canais radiculares ovais. O sistema WO foi mais eficaz no aumento do volume de canal, entretanto, isso não afetou as áreas instrumentadas. O sistema OS teve a correlação mais forte entre o aumento de volume e área instrumentada, enquanto o sistema EPL foi o mais rápido.

Endodontic retreatment of dens invaginatus: clinical and radiographic report

Introduction and Objective: Dens invaginatus, a developmental malformation resulting from the invagination of enamel, has been associated with increased prevalence of pulpal and periapical diseases. Case report:This anomaly is classified according to the severity (types I-III). Management of type III dens invaginatus is complex and an endodontic challenge. This case report describes the management of the endodontic retreatment type III dens invaginatus in a maxillary lateral incisor. Conclusion: Nonsurgical treatment can be effective to treat type III dens invaginatus. CBCT is an important diagnostic tool that allowed the management of such case.

Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.

Análise ex vivo da influência do preparo cervical na determinação do comprimento de trabalho por três diferentes localizadores apicais eletrônicos / Ex vivo analysis of the influence of cervical preparation in determining the working length by three different apex electronic locators

Introdução: Atualmente uma geração nova de localizadores apicaiseletrônicos (electronic apex locators ? EALs) passou a utilizar correntesalternadas com frequências baixas, em que o efeito do potencial de polarização do eletrodo é evitado, dando mais precisão para os valoresmedidos. Objetivo: O objetivo deste estudo foi avaliar ex vivo a influênciado preparo do terço cervical na eficácia de três localizadores apicaisquando comparados com o exame radiográfico. Material e métodos:Foram utilizados 20 pré-molares inferiores de canais únicos e retos. Osespécimes tiveram inicialmente o comprimento de trabalho estabelecidoa 1 mm do ápice radicular pelo método visual. Na sequência os dentesforam submetidos ao método radiográfico e aos localizadores apicaisRoot ZX, Root ZX II e Novapex, definindo dessa forma dados numéricosdo grupo controle, radiográfico e localizadores apicais. A medição foifeita antes e após o preparo do terço cervical, com o instrumento SXdo sistema Protaper. Resultados: Após a obtenção dos dados, estesforam submetidos a análise estatística, e não se encontrou diferençaestatisticamente significante entre os grupos experimentais estudados.Conclusão: Os localizadores apicais pesquisados provaram ser precisospara a realização da odontometria durante a terapia endodôntica.

Introduction: Currently, a new generation of electronic apex locators(EALs) started to use alternating current at low frequencies, where theeffect of polarization potential of the electrode is avoided, giving moreprecision to the measured values. Objective: The aim of this studywas to evaluate ex vivo the influence of the preparation of the cervicalthird in the effectiveness of three apex locators when compared to theradiographic examination. Material and methods: 20 mandibularpremolars with single straight canals were used. At first the specimenshad the working length established at 1 mm from the apex through thevisual method. Then teeth were submitted to the radiographic methodand to the Root ZX, Root ZX II and Novapex apex locators, therebysetting data in the control group, radiographic and apex locators. Themeasurement was performed before and after the preparation of thecervical third, with the SX instrument of the Protaper system. Results:After obtained, data was submitted to statistical analysis, and nostatistically significant difference was found between the experimentalgroups studied. Conclusion: The apex locators studied proved tobe accurate to perform the root canal measuring during endodontictherapy.

Multiple taurodontism: the challenge of endodontic treatment.

This article describe a rare case of multiple taurodontism involving all molars in a 17-year-old male. Volumetric cone-beam computed tomography was used to investigate internal and external root morphology, including that of a maxillary first molar which required endodontic treatment and retreatment. Medical history was not contributory; however, Klinefelter syndrome was the diagnostic hypothesis in this case.

Capacidade seladora do agregado de trióxido mineral em perfurações de furca após irrigação final com diferentes soluções / Mineral trioxide aggregate sealing ability in furcal perforations after final irrigation with different solutions

Introdução - O presente estudo avaliou a influência de diferentes soluções irrigadoras na capacidade seladora do MTA quando utilizado em perfurações na região de furca de molares inferiores extraídos. Material e Métodos - Trinta molares inferiores humanos foram submetidos à abertura endodôntica convencional e tiveram o centro do assoalho perfurado propositalmente com broca esférica diamantada. Os espécimes foram divididos em 3 grupos (n = 10) de acordo com as soluções utilizadas: G1-soro fisiológico; G2-NaOCl a 1% e G3-EDTA 17%. Foi realizado o preenchimento da perfuração com MTA Angelus cinza e o selamento coronário provisório. Em seguida,realizou-se a impermeabilização da superfície externa, exceto na região de furca, e imersão em corante nanquim por 72 horas. A mensuração, em milímetros, da infiltração linear foi realizada por meio do software Image Tool. Resultados - Os resultados demonstraram que a solução de EDTA 17% permitiu menor infiltração, sendo estatisticamente diferente do NaOCl a 1% e do soro fisiológico(p < 0,05). Conclusão - De acordo com os resultados, pode-se concluir que a solução de EDTA parece ser a melhor escolha para irrigação final em casos de perfuração de furca.

Introduction - This study assessed the influence of different final irrigation on the sealing ability of MTA-Angelus in furcal perforations of extracted human molar teeth. Material and Methods - Thirty human mandibular molars were conventionally accessed and perforations were purposely created in the center of the pulp chamber floor by using a round diamond bur. The specimens were divided into 3 groups (n = 10) according to the irrigation: G1-saline solution; G2-1% sodium hypochlorite; G3-17% EDTA. Teeth were repaired with Angelus'gray MTA and the crowns were sealed.Then the impermeabilization of the external surface was made except in the furcal region, and they were immersed into Indian Ink for 72 hours. The leakage was measured, in millimeters, by Image Tool software. Results - The results showed lower leakage index for EDTA group that was statistically different from 1% NaOCl and saline solution groups (p < 0.05). Conclusion - According to results it can be concluded that in cases of furcal perforation EDTA solution seems to be the best choice of final irrigation.