Long-term investigation of phosphorus removal by iron electrocoagulation in small-scale wastewater treatment plants.

Small-scale wastewater treatment plants (SWTPs), called Johkasou, are widely used as decentralized and individual wastewater treatment systems in sparsely populated areas in Japan. Even in SWTPs, nutrients should be removed to control eutrophication. An iron electrolysis method is effective to remove phosphorus chemically in SWTPs. However, it is necessary to determine the precise conditions under which phosphorus can be effectively and stably removed in full scale SWTPs for a long period. Therefore, long-term phosphorus removal from SWTPs was investigated and optimum operational conditions for phosphorus removal by iron electrolysis were analyzed in this study. Efficient phosphorus removal can be achieved for a long time by adjusting the amount of iron against the actual population equivalent. The change of the recirculation ratio had no negative effect on overall phosphorus removal. Phosphorus release to the bulk phase was prevented by the accumulated iron, which was supplied by iron electrolysis, resulting in stable phosphorus removal. The effect of environmental load reduction due to phosphorus removal by iron electrolysis was greater than the cost of power consumption for iron electrolysis.

Improvement of phosphorus removal by calcium addition in the iron electrocoagulation process.

Small-scale wastewater treatment plants (SWTPs) are widely used as decentralized wastewater treatment systems in sparsely populated areas of Japan. Iron electrolysis, an electrocoagulation technology, is installed in these SWTPs for phosphorus removal. Phosphorus can be removed via the formation of an insoluble compound containing phosphate and iron, such as FePO4; however, it was necessary to determine the conditions under which phosphorus can be effectively and stably removed in actual SWTPs. According to previous studies using iron compounds, improved phosphorus removal was obtained by Ca addition. It is therefore thought that calcium addition may also be effective in improving the phosphorus removal during iron electrolysis in SWTPs. It is also important to determine the chemical state of iron to understand the phosphorus removal mechanism during iron electrolysis. In this study, laboratory-scale batch experiments with the iron electrolysis method were conducted to investigate the effect of phosphorus removal using treated wastewater from actual SWTPs without or with Ca addition. The results indicated that the addition of Ca improved the phosphorus removal performance. Furthermore, phosphorus removal was inhibited in the presence of high dissolved organic carbon (DOC). The X-ray absorption fine structure measurements of the produced particulates in the experiments showed no substantial change in the chemical state of iron without or with Ca addition. The statistical analyses revealed the range of improving or inhibiting effects on phosphorus removal due to the Ca and DOC. Thus, the results of this study provided useful information pertaining to the influence of coexisting substances on phosphorus removal and the chemical state of iron in the produced particulates.

Control of membrane fouling in membrane bioreactor process by coagulant addition.

The control of membrane fouling is an essential issue in membrane bioreactor (MBR) process. It has been recognized that the most important factors that affect membrane fouling are presence of soluble microbial products (SMP) and extracellular polymeric substances (EPS) in a reactor. The objective of this study was to examine the effect of the coagulant addition on the membrane fouling in the MBR process. Accordingly, laboratory scale batch experiments and MBR experiments were conducted using coagulant. In batch experiments, effective SMP removal and control of EPS release were observed by coagulant addition. Fe coagulant was slightly more effective than Al coagulant in the addition of same mole amount. Therefore, Fe was used as coagulant in MBR experiments. In MBR experiments, Fe solutions of 0 mg/L, 2,260 mg/L and 4,520 mg/L were added into the tanks (Run1, Run2 and Run3, respectively) with the flow rate of 200 mL/d. COD removal efficiencies of 97% and phosphorus removal efficiencies of 92% were observed by Fe addition in the MBR experiment. Membrane fouling occurred more often in Run1 than in the other two Runs. Membrane was cleaned 18, 9 and 5 times in Run1, Run2 and Run3, respectively during 40 days runs. This suggested that the membrane fouling was reduced by the coagulant addition. The protein and carbohydrate concentrations of the SMP in the fraction of 1 microm-0.4 microm in Run2 and Run3 were significantly lower than that in Run1 and the particle size of the activated sludge was obviously increased in Run2 and Run3. These results suggested that the coagulant addition is effective to control the membrane fouling.

Reduction of coagulant amount added to activated sludge for phosphorus removal.

Adding coagulant to the activated sludge process is effective in maintaining the stability of phosphorus removal. However, the precise mechanisms of the reaction and behavior of coagulants and phosphorus are not well known. By introducing a new phosphorus removal model (PRM), the behavior of coagulant and phosphorus in the process could be described. The experimental data of the effluent phosphorus concentration and Fe content in the activated sludge agreed with the values calculated by PRM. The amount of coagulant addition to the activated sludge process for phosphorus removal is reduced with the enhanced biological phosphorus removal process. It is suggested that the amount of reduction is determined by using PRM.

[Determination of tocopherols in oils of mixed tocopherol as food additive using reverse-phase high-performance liquid chromatography].

Simultaneous determination of four tocopherols was developed using reverse-phase high-performance chromatography with a mixture of methanol and water (88:12) as a mobile phase. The alpha-, beta-, gamma- and delta-tocopherols in oils of mixed tocopherol as food additive were determined. It is clarified that the proposed method is useful for the quality control of food additive.

[Estimated production by the official inspection of coal-tar dyes (including dye aluminum lakes) in 1997].

The number of official inspection of coal-tar dyes and their lakes from April in 1997 till March in 1998 were 571 in total. The quantity which passed inspection amounted to 160.3 ton in Japan. The production of color in each month was summarised in Table 1, and by each producing company in Table 2. The food coal-tar dye produced in the largest quantity was Food Yellow No.4, occupying 39.8% in this period.

[Estimated production by the official inspection of coal-tar dyes (including dye aluminum lakes) in 1996].

The number of official inspection of coal-tar dyes and their lakes from April in 1996 till March in 1997 were 581 in total. The quantity which passed inspection amounted to 164.5 ton in Japan. The production of color in each month was summarised in Table1, and by each producing company in Table2. The food coal-tar dye produced in the largest quantity was Food Yellow No. 4, occupying 43.4% in this period.

[Determination and survey of starting materials, intermediates, and subsidiary colors in food color of azo dye by high performance liquid chromatography].

A method for determination of starting materials, intermediates and subsidiary colors in food color of azo dye was developed by use of HPLC. The following conditions were used for analysis: column, L-column ODS (4.6 mm phi x 250 mmL); mobile phase, 0.02 M ammonium acetate (A), acetonitrile (B); concentration gradient, perform the linear concentration gradient from A:B (100:0) to (60:40) for 40 min; detection, starting materials and intermediates at 239 nm, and subsidiary colors at 510 nm. Standard material, domestic product and imported product were analyzed by the present HPLC method and impurities were measured. Recoveries of each impurity from azo dye averaged 99.1-103.5%. The detection limit was 0.05 microgram/g for each impurity.

[Estimated production by the official inspection of coal-tar dyes (including dye aluminum lakes) in 1995].

The number of official inspection of coal-tar dyes and their lakes from April in 1995 till March in 1996 were 580 in total. The quantity which passed inspection amounted to 166.4 ton in Japan. The production of color in each month was summarised in Table 1, and by each producing company in Table 2. The food coal-tar dye produced in the largest quantity was Food Yellow No.4, occupying 43.9% in this period.

[Studies on "Fast Green FCF Standard" for the dye standard on the National Institute of Health Sciences].

The raw material for Fast Green FCF was tested for preparation of the "Fast Green FCF Standard (C.I. 42053)". Analytical data obtained were as follows: paper chromatography, only one spot is observed; arsenic content, 0.38 microgram/g; chloride content, 0.11%; sulfate content, 3.30%; heavy metals, lead, 8.0 micrograms/g, manganese, 28.1 micrograms/g, and chromium, 1.6 micrograms/g; infrared spectra, 1575 cm-1, 1169 cm-1, and 1033 cm-1; loss on drying, 2.39%; assay, 93.0% by the titanium trichloride titration. Based on the above results, the raw material was authorized as the Dye Standard of National Institute of Health Sciences.

Neurologic features of chronic minamata disease (organic mercury poisoning) and incidence of complications with aging.

To elucidate the neurologic features of chronic Minamata disease, and the incidence of complications with aging, we studied 80 patients with documented Minamata disease (organic mercury poisoning) from 1986 to 1994 (mean age: 63 years). Of the cardinal neurologic findings, sensory impairment was seen with highest frequency in 98.8% of patients limited to the extremities in 86.3%. Impairment of lower extremity coordination was observed in 60%, constriction of the visual field in 51.9%, and retrocochlear hearing loss in 41%. To assess age-related complications, patients were separated into three groups by age: Group I (10 to 39 years); Group II (40 to 69 years); Group III (> or = 70 years). The incidences of hypertension and cerebrovascular diseases, organic ophthalmologic disorders (including cataracts), presbyacusis, and cervical spondylosis deformans increased significantly with age. Compared with a preceding survey (1981 to 1985, 171 patients, mean age: 63.5 years), the incidences of complicated hypertension and cataracts had decreased, whereas those of cerebrovascular disease and retinitis pigmentosa remained unchanged. The incidences of abnormal brain computed tomography (CT), presbyacusis, cervical spondylosis deformans, and positive tests for urine sugar also increased. The incidences of these complications other than retinitis pigmentosa were similar to those in the general population. These results accurately reflect the recent epidemiological disease tendencies in Japan toward a decreased incidence of hypertension and an increased incidence of diabetes.

Neurologic features of chronic Minamata disease (organic mercury poisoning) certified at autopsy.

To better understand the neurologic events related to chronic Minamata disease (organic mercury poisoning), we studied data from 77 patients with Minamata disease as certified at autopsies performed from 1976 to 1994 (mean age: 72.3 years). Major neurologic findings included: sensory impairment in 80.5% of the patients which was limited to the extremities in 42.9%. Impairment of lower extremity coordination was present in 35.8% of the patients, constriction of the visual fields in 28.8%, and retrocochlear hearing loss in 15.3%. There was no correlation between the degree of cerebellar incoordination and the methylmercury concentration in the cerebellum. Compared with the classic type of Minamata disease, the incidence of major neurologic findings was markedly decreased. In light of these findings, supplemental examinations including brain computed tomography (CT), magnetic resonance imaging (MRI), short latency somatosensory evoked potential (SSEP), or tremogram may be necessary to clinically diagnose Minamata disease, especially in atypical or mild cases.

[Estimated production by the official inspection of coal-tar dyes (including dye aluminum lakes) in 1994].

The number of official inspection of coal-tar dyes and their lakes from April in 1994 till March in 1995 were 635 in total. The quantity which passed inspection amounted to 186 ton in Japan. The production of color in each month was summarised in Table 1, and by each producing company in Table 2. The food coal-tar dye produced in the largest quantity was Food Yellow No.4, occupying 40.7% in this period.

Computed tomography in fetal methylmercury poisoning.

Cerebral computed tomography was studied in eight patients with fetal methylmercury poisoning. All patients had evidence of prenatal exposure to methylmercury and showed psychomotor retardation. All of them had abnormal computed tomography findings. The most common finding was sulcal and ventricular enlargement, with or without cisternal enlargement. These findings indicated generalized cortical and subcortical maldevelopment. The distribution of sulcal and fissural enlargement did not show specific dominance in central sulcus, calcarine fissure or cerebellum. This distribution contrasts with the location of focal atrophy present in adult methylmercury poisoning. The computed tomography findings were relatively mild compared with the clinical features and were not distinct from those in cerebral palsy.

Truncal hypesthesia in patients with Minamata disease.

Patients with Minamata disease (methylmercury intoxication) usually suffer from the glove and stocking type hypesthesia in the extremities. Recently it is believed that the sensory disturbance of the disease may be a manifestation of the cerebral involvement. The aim of this study was to determine the pain threshold of their extremities and body by algesimeter. The results showed that a majority of the patients had elevated pain thresholds in the body and the extremities, and suggest that the pain impairment is not responsible for the peripheral nerve involvement.

[Sexual hormone on the human mammary carcinoma cells in vitro (author's transl)]. / Sexualhormone auf die Kulturen menschlicher Mammakrebszellen

PIP: The effects of estradiol-17 beta and testosterone on cultures of human breast cancer cells from 10 patients are described. Testosterone produced a significant decrease in the rate of cell proliferation of sex-chromatin-positive cells, while no effect of estrogen could be observed. Sex-chromatin-negative cells appeared to be independent of the 2 hormones. A significant decrease in the number of cells in cultures derived from postmenopausal patients was noted after testosterone application. Sex-chromatin-positive cells in cultures treated with either hormone were frequently abnormal in shape, with pycnotic and lytic aspects and nuclear abnormalities; sex-chromatin-negative cells showed similar abnormalities even more frequenlty.^ieng