MRS-measured glutamate versus GABA reflects excitatory versus inhibitory neural activities in awake mice.

To assess if magnetic resonance spectroscopy (MRS)-measured Glutamate (Glu) and GABA reflect excitatory and inhibitory neural activities, respectively, we conducted MRS measurements along with two-photon mesoscopic imaging of calcium signals in excitatory and inhibitory neurons of living, unanesthetized mice. For monitoring stimulus-driven activations of a brain region, MRS signals and mesoscopic neural activities were measured during two consecutive sessions of 15-min prolonged sensory stimulations. In the first session, putative excitatory neuronal activities were increased, while inhibitory neuronal activities remained at the baseline level. In the second half, while excitatory neuronal activities remained elevated, inhibitory neuronal activities were significantly enhanced. We assessed regional neurochemical statuses by measuring MRS signals, which were overall in accordance with the neural activities, and neuronal activities and neurochemical statuses in a mouse model of Dravet syndrome under resting condition. Mesoscopic assessments showed that activities of inhibitory neurons in the cortex were diminished relative to wild-type mice in contrast to spared activities of excitatory neurons. Consistent with these observations, the Dravet model exhibited lower concentrations of GABA than wild-type controls. Collectively, the current investigations demonstrate that MRS-measured Glu and GABA can reflect spontaneous and stimulated activities of neurons producing and releasing these neurotransmitters in an awake condition.

Generation and compression of an intense infrared white light continuum in YAG irradiated by picosecond pulses.

An intense white light (WL) continuum from 1600 to 2400 nm is generated in a 20-mm-long YAG irradiated by 1-ps, 1030-nm pulses. Long filamentation formed in the YAG is proven to be responsible for the enhancement of the longer-wavelength spectral part of the WL. The WL is compressed down to 24.6 fs ( 3.9 cycles at 1900 nm) after optical parametric chirped-pulse amplification in a lithium niobate crystal near degeneracy, confirming that its spectral phase is well behaved. The pulse compression experiment reveals that the group delay introduced in the WL generation process is dominated by the dispersion of YAG.

Gyro-Sensor-Based Vibration Control for Dynamic Humanoid-Robot Walking on Inclined Surfaces.

An efficient motor-control system for stable walking of the lightweight humanoid robot KONDO KHR-3HV on inclined surfaces is investigated. The motor-control system is based on the angular velocity of the pitch motion of the robot, which is detected by a gyro sensor attached to the robot torso and referred to as the angular-pitch velocity. The robot gait is analyzed for different downslopes with and without the motor-feedback control. A novel method of frequency-domain analysis of the angular-pitch velocity is proposed for explaining the reasons behind the instabilities of dynamic humanoid-robot walking on inclined surfaces. The results show, that a nonlinear nature of the motor torque, due to a force induced by the slope, gives rise to harmonics of the fundamental walking frequency of 1.73 Hz. These harmonics are the origin of the unstable robot walking. Additionally, the feedback-gain parameters KA and KH affect the amplitudes of the harmonics, which give rise to vibrations at a higher surface inclination. Increased surface friction allows a reduction of the feedback gain, which reduces this specific contribution to the harmonics and thus stabilizes the robot. To improve the walking stability on inclined surfaces, it is found that the damped natural frequency of the motor-control system must be kept lower than the fundamental walking frequency.

Optical parametric oscillator pumped by a 100-kHz burst-mode Yb-doped fiber laser.

We demonstrate an optical parametric oscillator pumped at a repetition rate of 100 kHz by a burst-mode Yb-doped fiber laser. Pulse energies of 1.5 µJ were generated with five 4.8-µJ pump pulses. Pulse-to-pulse fluctuations could be suppressed even when only five pump pulses were used. The measured pulse length was 190 fs, which was considerably shorter than the 350-fs pump pulse length. The burst-mode operation is an easy and powerful way to increase the pulse energies of optical parametric oscillators pumped with femtosecond pulses.

Sub-picosecond regenerative amplifier of Yb-doped Y(2)O(3) ceramic thin disk.

We have developed a 1 kHz regenerative amplifier using an Yb:Y(2)O(3) ceramic thin disk as the gain medium. Furthermore, the thermal conductivity and heat generation property of Yb:Y(2)O(3) ceramic were investigated. In the developed regenerative amplifier, a laser beam is bounced off the thin disk six times in each round trip. The output energy is over 2 mJ, spectral bandwidth is 1.8 nm at FWHM, and pulse duration after pulse compression is 0.9 ps.

Yb:YAG thin-disk chirped pulse amplification laser system for intense terahertz pulse generation.

We have developed a 1 kHz repetition picosecond laser system dedicated for intense terahertz (THz) pulse generation. The system comprises a chirped pulse amplification laser equipped with a Yb:YAG thin-disk amplifier. At room temperature, the Yb:YAG thin-disk regenerative amplifier provides pulses having energy of over 10 mJ and spectral bandwidth of 1.2 nm. The pulse duration achieved after passage through a diffraction grating pair compressor was 1.3 ps. By employing this picosecond laser as a pump source, THz pulses having a peak frequency of 0.3 THz and 4 µJ of energy were generated by means of optical rectification in an Mg-doped LiNbO3 crystal.

Contact grating device with Fabry-Perot resonator for effective terahertz light generation.

A novel design for a contact grating device with an incorporated Fabry-Perot resonator is proposed for high-power terahertz (THz) light generation. We deposited a multilayer consisting of Ta(2)O(5) and Al(2)O(3) on a magnesium-doped stoichiometric LiNbO(3) substrate and fabricated grating grooves on the outermost layer. The multilayer was designed such that conditions for a Fabry-Perot resonator were satisfied for light diffracted by the grating. Consequently, the fraction of light transmitted into the LiNbO(3) substrate, i.e., the diffraction efficiency, was enhanced by the resonator. The diffraction efficiency of the fabricated device was 71%, which is close to the calculated value of 78% from the optimized design. THz light generation was also demonstrated with the contact grating device. The THz output of 0.41 µJ was obtained using near-infrared pump light of 2.7 mJ.

Experimental verification of femtosecond laser ablation schemes by time-resolved soft x-ray reflective imaging.

Pump and probe reflective imaging using a soft x-ray laser probe was applied to the observation of the early stage of femtosecond laser ablation process on platinum. In strongly excited area, drastic and fast reflectivity drop was observed. In moderately excited area, the decay of the reflectivity is slower than that in the strongly excited area, and the reflectivity reaches its minimum at t = 160 ps. In weakly excited area, laser-induced reflectivity change was not observed. In addition, the point where the reflectivity dip was observed at t = 10 ps and t = 40 ps, coincides with the position of the edge of reflectivity drop at t = 160 ps. These results give the critical information about the femtosecond laser ablation.

Laser ceramic materials for subpicosecond solid-state lasers using Nd3+-doped mixed scandium garnets.

We have successfully developed and demonstrated broadband emission Nd-doped mixed scandium garnets based on laser ceramic technology. The inhomogeneous broadening of Nd(3+) fluorescence lines results in a bandwidth above 5 nm that is significantly broader than that for Nd:YAG and enables subpicosecond mode-locked pulse durations. We have also found the emission cross section of 7.8 × 10(-20) cm(2) to be adequate for efficient energy extraction and thermal conductivity of 4.7 W/mK from these new Nd-doped laser ceramics. The new laser ceramics are good candidates for laser host material in a diode-pumped subpicosecond laser system with high efficiency and high repetition rate.

Single-shot picosecond interferometry with one-nanometer resolution for dynamical surface morphology using a soft X-ray laser.

Using highly coherent radiation at a wavelength of 13.9 nm from a Ag-plasma soft X-ray laser, we constructed a pump-and-probe interferometer based on a double Lloyd's mirror system. The spatial resolutions are evaluated with a test pattern, showing 1.8-mum lateral resolution, and 1-nm depth sensitivity. This instrument enables a single-shot observation of the surface morphology with a 7-ps time-resolution. We succeeded in observing a nanometer scale surface dilation of Pt films at the early stage of the ablation process initiated by a 70 fs near infrared pump pulse.

High-spatiotemporal-quality petawatt-class laser system.

We have developed a femtosecond high-intensity laser system that combines both Ti:sapphire chirped-pulse amplification (CPA) and optical parametric CPA (OPCPA) techniques and produces more than 30 J broadband output energy, indicating the potential for achieving peak powers in excess of 500 TW. With a cleaned high-energy seeded OPCPA preamplifier as a front end in the system, for the compressed pulse without pumping the final amplifier, we found that the temporal contrast in this system exceeds 10(10) on the subnanosecond time scales, and is near 10(12) on the nanosecond time scale prior to the peak of the main femtosecond pulse. Using diffractive optical elements for beam homogenization of a 100 J level high-energy Nd:glass green pump laser in a Ti:sapphire final amplifier, we have successfully generated broadband high-energy output with a near-perfect top-hat-like intensity distribution.

A quantitative measure for discriminating between self and non-self antigens in immune response.

We present a new theory of how lymphocyte-antigen interaction is governed. We present 'chronicity', a quantitative record of previous lymphocyte-antigen interactions, which is used to regulate lymphocyte behavior. When the chronicity of a lymphocyte increases with the interaction and gets beyond the lower threshold, the lymphocyte can proliferate. Non-self antigens cause lymphocyte proliferation which destroys the antigen. However, self antigens are not destroyed. When the chronicity gets beyond the upper threshold, the lymphocytes get in the tolerance state ensuring non-destruction of self antigens. The discrimination between self and non-self results from the difference in the termination process between self and non-self antigens, caused by the difference in the frequency between interaction of lymphocyte with both antigens.

Demonstration of a highly coherent 13.9 nm x-ray laser from a silver tape target.

A highly coherent 13.9 nm x-ray laser (XRL) is generated under an oscillator-amplifier configuration using a new tape target system and a driver laser system with a 0.1 Hz repetition rate. The output energy is comparable to the XRL generated with a silver-deposited slab target, and the pointing stability using the new tape target system is better than conventional slab targets.

Observation of a plasma waveguide in a preformed plasma pumped by double-pulse laser irradiation for the efficient soft x-ray amplification.

A molybdenum slab target was irradiated by double pulses of a line-focused chirped-pulse-amplification Nd:glass laser. A probe beam was injected in the plasma in the longitudinal direction, and we found that the probe beam was guided in the plasma with the plasma length of up to 4.3 mm at the time of 0.8-1.5 ns after the peak of the second pulse. Comparison with hydrodynamics code implied that the electron-density dip or flat region was formed, which was favorable for the efficient soft x-ray lasing under the coupling of the seed x-ray and the x-ray amplifier.

Development of a chirped pulse amplification laser with zigzag slab Nd:glass amplifiers dedicated to x-ray laser research.

A chirped pulse amplification laser with zigzag slab Nd:glass amplifiers dedicated to x-ray laser research is described. The laser provides a 1.6 ps duration pulse with approximately 7 J energy at a repetition rate of 0.1 Hz. In the power amplifier system, laser light is amplified in a two-step manner: The first step is image-relayed multipass amplification up to approximately 1 J with a 10 mm x 10 mm beam. The second step is double-pass amplification up to >10 J with a 10 mm x 90 mm beam. By using this laser system, the saturated amplification of the Ni-like Ag laser at a wavelength of 13.9 nm has been successfully demonstrated.

Population kinetics on K alpha lines of partially ionized Cl atoms.

A population kinetics code was developed to analyze K alpha emission from partially ionized chlorine atoms in hydrocarbon plasmas. Atomic processes are solved under collisional-radiative equilibrium for two-temperature plasmas. It is shown that the fast electrons dominantly contribute to ionize the K-shell bound electrons (i.e., inner-shell ionization) and the cold electrons to the outer-shell bound ones. Ratios of K alpha lines of partially ionized atoms are presented as a function of cold-electron temperature. The model was validated by observation of the K alpha lines from a chlorinated plastic target irradiated with 1 TW Ti:sapphire laser pulses at 1.5 x 10(17) W/cm(2), inferring a plasma temperature of about 100 eV on the target surface.