Holothurians play an important role in mitigating the impacts of aquaculture on sediment conditions.

As deposit feeders contribute to bioremediation and nutrient recycling in sediments, positively impacting water and sediment quality, holothurians are candidate organisms for multitrophic aquaculture. This study aimed to investigate the potential of Holothuria poli to reduce the environmental footprint of fish farms through a benthocosm experiment. The experimental setup included four benthocosms with holothurians(H+) and four without (H-). The 58-day experiment included two phases: constant organic enrichment and recovery. In order to simulate the organic enrichment sediment conditions under a typical fish farm, a mixture of fish feces and fish feed pellets was added. Results showed that holothurians effectively reduced organic matter and H2S and increased redox, RPD depth and sediment oxygenation, thereby preventing anoxic conditions. Also, during the recovery phase, holothurians facilitated rapid sediment recovery, while the sediments without holothurians remained organic-enriched until the end of the experiment. The study emphasizes the significance of holothurians in mitigating the impacts of aquaculture on sediment conditions and so promoting environmental sustainability.

Contribution of holothuroids to the bioremediation and stimulation of the benthic microbial metabolism in aquaculture.

The implementation of sustainable system designs in aquaculture comprises the biological treatment of pollutants. In order to test the potential of Holothuria (Roweothuria) poli as a candidate for bioremediation of aquaculture wastes, the effect of this species on the benthic microbial activity was studied. Two experimental treatments, with and without holothuroids, were allocated in two benthocosms, each one containing four replicate tanks with undisturbed sediment and a volume of water above it. It was anticipated that H. poli could affect the overall assimilative capacity of the aquaculture effluent system either through the consumption of particulate organic matter or through the stimulation of benthic microbial metabolism. Indeed, the results indicated that the microbial activity related to the mineralization of the N and C was significantly different between the treatments verifying our hypothesis that deposit-feeders that move above or inside the sediment matrix can accelerate the depletion of organic matter through bioturbation.

Changes in (micro and macro) plastic pollution in the sediment of three sandy beaches in the Eastern Mediterranean Sea, in relation to seasonality, beach use and granulometry.

Smaller sized plastics (microplastics or MPs <5 mm) are ubiquitous in nature and have been found to interact in diverse ways with most biotic and abiotic systems globally. Most MPs in the seas have a land-based source, however, little is known about how the transfer occurs. In our study, we used three sandy beaches to describe the process of how MPs travel from accumulation points at the backshore of the beach to the sea, and vice versa. MPs differed significantly in all three beaches (both in quantitative and qualitative terms) between the summer and the winter samplings. During the summer, heavy MPs are the majority, while during the winter, lightweight microplastics are predominant, and the ratio of heavy per lightweight MPs is affected by the sediment median diameter after the summer sampling. Macroplastics follow a similar pattern to MPs and appear to provide a source of MPs for the sea.

Prokaryotic and eukaryotic microbial community responses to N and P nutrient addition in oligotrophic Mediterranean coastal waters: Novel insights from DNA metabarcoding and network analysis.

The effects of the abrupt input of high quantities of dissolved inorganic nitrogen and phosphorus on prokaryotic and eukaryotic microbial plankton were investigated in an attempt to simulate the nutrient disturbances caused by eutrophication and climate change. Two nutrient levels were created through the addition of different quantities of dissolved nutrients in a mesocosm experiment. During the developed blooms, compositional differences were found within bacteria and microbial eukaryotes, and communities progressed towards species of faster metabolisms. Regarding the different nutrient concentrations, different microbial species were associated with each nutrient treatment and community changes spanned from the phylum to the operational taxonomic unit (OTU) level. Network analyses revealed important differences in the biotic connections developed: more competitive relationships were established in the more intense nutrient disturbance and networks of contrasting complexity were formed around species of different ecological strategies. This work highlights that sudden disturbances in water column chemistry lead to the development of entirely different microbial food webs with distinct ecological characteristics.

Molluscan indicator species and their potential use in ecological status assessment using species distribution modeling.

Marine habitat assessment using indicator species through Species Distribution Modeling (SDM) was investigated. The bivalves: Corbula gibba and Flexopecten hyalinus were the indicator species characterizing disturbed and undisturbed areas respectively in terms of chlorophyll a concentration in Greece. The habitat suitability maps of these species reflected the overall ecological status of the area. The C. gibba model successfully predicted the occurrence of this species in areas with increased physical disturbance driven by chlorophyll a concentration, whereas the habitat map for F. hyalinus showed an increased probability of occurrence in chlorophyll-poor areas, affected mainly by salinity. We advocate the use of C. gibba as a proxy for eutrophication and the incorporation of this species in monitoring studies through SDM methods. For the Mediterranean Sea we suggest the use of F. hyalinus in SDM as an indicator of environmental stability and a possible forecasting tool for salinity fluctuations.

Benthic pelagic coupling in a mesocosm experiment: Delayed sediment responses and regime shifts.

A mesocosm experiment was performed to study benthic-pelagic coupling under a eutrophication gradient. Nine mesocosms were deployed in the facilities of the Hellenic Center for Marine Research in Crete, in the Eastern Mediterranean. The mesocosms were 4m deep, containing 1.5m3 of coastal water and, at the bottom, they included 85l of undisturbed sediment, collected from a semi-impacted area in the port of Heraklion, Crete. A eutrophication gradient was created by adding nutrients in the water column (Low and High) and the experiment lasted 58days. Water column and sediment environmental variables were measured at regular intervals. The results indicate that sedimentation caused by eutrophication in the water column affected sediment geochemical variables but in most cases a time lag was observed between the trophic status of the water column and the response of the sediment. Additionally, in the High eutrophication treatment, several fluctuations were observed and the system did not recover within the experimental duration, as opposed to the Low treatment which showed fewer fluctuations and signs of recovery.

Environmental variability and heavy metal concentrations from five lagoons in the Ionian Sea (Amvrakikos Gulf, W Greece).

BACKGROUND: Coastal lagoons are ecosystems of major importance as they host a number of species tolerant to disturbances and they are highly productive. Therefore, these ecosystems should be protected to ensure stability and resilience. The lagoons of Amvrakikos Gulf form one of the most important lagoonal complexes in Greece. The optimal ecological status of these lagoons is crucial for the well-being of the biodiversity and the economic prosperity of the local communities. Thus, monitoring of the area is necessary to detect possible sources of disturbance and restore stability. NEW INFORMATION: The environmental variables and heavy metals concentrations, from five lagoons of Amvrakikos Gulf were measured from seasonal samplings and compared to the findings of previous studies in the area, in order to check for possible sources of disturbance. The analysis, showed that i) the values of the abiotic parameters vary with time (season), space (lagoon) and with space over time; ii) the variability of the environmental factors and enrichment in certain elements is naturally induced and no source of contamination is detected in the lagoons.

One Step forward: Benthic Pelagic Coupling and Indicators for Environmental Status.

A large data set from the Eastern Mediterranean was analyzed to explore the relationship between seawater column variables and benthic community status. Our results showed a strong quantitative link between the seawater column variables (Chlorophyll a and Eutrophication Index) and various indicators describing benthic diversity and community composition. The percentage of benthic opportunistic species increased significantly in the stations with high trophic status of the seawater column and so did the strength of the coupling between values of seawater column and benthic indicators. The Eutrophication Index threshold level of 0.85, separating the "Bad and Poor" from "Moderate to High" conditions could serve as an acceptable critical value above which there is a readily observable change in benthic community composition.

Polytraits: A database on biological traits of marine polychaetes.

The study of ecosystem functioning - the role which organisms play in an ecosystem - is becoming increasingly important in marine ecological research. The functional structure of a community can be represented by a set of functional traits assigned to behavioural, reproductive and morphological characteristics. The collection of these traits from the literature is however a laborious and time-consuming process, and gaps of knowledge and restricted availability of literature are a common problem. Trait data are not yet readily being shared by research communities, and even if they are, a lack of trait data repositories and standards for data formats leads to the publication of trait information in forms which cannot be processed by computers. This paper describes Polytraits (http://polytraits.lifewatchgreece.eu), a database on biological traits of marine polychaetes (bristle worms, Polychaeta: Annelida). At present, the database contains almost 20,000 records on morphological, behavioural and reproductive characteristics of more than 1,000 marine polychaete species, all referenced by literature sources. All data can be freely accessed through the project website in different ways and formats, both human-readable and machine-readable, and have been submitted to the Encyclopedia of Life for archival and integration with trait information from other sources.

The pattern of change in the abundances of specific bacterioplankton groups is consistent across different nutrient-enriched habitats in Crete.

A common source of disturbance for coastal aquatic habitats is nutrient enrichment through anthropogenic activities. Although the water column bacterioplankton communities in these environments have been characterized in some cases, changes in α-diversity and/or the abundances of specific taxonomic groups across enriched habitats remain unclear. Here, we investigated the bacterial community changes at three different nutrient-enriched and adjacent undisturbed habitats along the north coast of Crete, Greece: a fish farm, a closed bay within a town with low water renewal rates, and a city port where the level of nutrient enrichment and the trophic status of the habitat were different. Even though changes in α-diversity were different at each site, we observed across the sites a common change pattern accounting for most of the community variation for five of the most abundant bacterial groups: a decrease in the abundance of the Pelagibacteraceae and SAR86 and an increase in the abundance of the Alteromonadaceae, Rhodobacteraceae, and Cryomorphaceae in the impacted sites. The abundances of the groups that increased and decreased in the impacted sites were significantly correlated (positively and negatively, respectively) with the total heterotrophic bacterial counts and the concentrations of dissolved organic carbon and/or dissolved nitrogen and chlorophyll α, indicating that the common change pattern was associated with nutrient enrichment. Our results provide an in situ indication concerning the association of specific bacterioplankton groups with nutrient enrichment. These groups could potentially be used as indicators for nutrient enrichment if the pattern is confirmed over a broader spatial and temporal scale by future studies.

Metals and other elements in tissues of wild fish from fish farms and comparison with farmed species in sites with oxic and anoxic sediments.

Farmed fish and wild fish aggregating in the vicinity of four Mediterranean fish farms with different environmental conditions were sampled. Levels of metals (including As and Se) were measured in the muscle, liver, gills, bone and intestine. The wild fish from sites with anoxic substrata accumulate metals (including As and Se) from the ambient habitat in their gills whereas those from sites with oxic substrata concentrate these elements through their diet in their intestine. Tissues of wild fish aggregating around farm cages accumulate a greater number of these elements and with higher concentrations than farmed fish. Habitat, diet, ecological needs, fat content of fish, and protein expression may play an important role in these element differences between fish species. Fe in flathead grey mullet, As in surmullet, rainbow wrasse, grey gurnard and picarel and Hg in bogue may pose a risk for human health. Farmed and wild fish are good sources of P, K, Cr and Se while flathead grey mullet, picarel and comber are excellent sources of Ca and Se.

Effects of fish farming on the biological and geochemical properties of muddy and sandy sediments in the Mediterranean Sea.

The aim of this paper was to test how benthic fauna and biogeochemical properties of sediment will vary in response to similar levels of organic enrichment (induced by fish farming) as a function of bottom-habitat type (i.e., mud versus seagrass/coarse sediments), distance from the enrichment source and depth. Our results showed that samples from silty sediments in the vicinity of fish farms have higher TOC and TON values, higher oxygen consumption, higher PO(4) release and lower benthic diversity. In this context muddy sites are more likely to be identified as impacted/critical, than coarse sediment ones.