New CeF3-ZnO nanocomposites for self-lighted photodynamic therapy that block adenocarcinoma cell life cycle.

AIM: To synthesize and characterize the performances of a new all-inorganic nanocomposite (NC) for self-lighted photodynamic therapy against cancer. This NC could allow radiotherapy doses to be reduced, as it enhances the effects of x-rays, generating cytotoxic reactive oxygen species as singlet oxygen. MATERIALS & METHODS: The proposed NC combines CeF3 and ZnO; CeF3 absorbs 6-MeV x-rays and activates the photosensitizer ZnO. Characterization is performed by transmission electron microscopy (TEM), scanning-TEM, energy dispersive x-ray spectrometry and fluorescence spectroscopies. Efficiency on human adenocarcinoma cells (A549) was tested by fluorescence spectroscopy, cytofluorimetry, viability assays, clonogenic assays, cell cycle progression assays. RESULTS: NC blocks A549's cell cycle before mitosis in the dark. Upon low-dose x-ray irradiation (2 Gy), reactive oxygen species/singlet oxygen are generated, further blocking cell cycle and reducing viability by 18% with respect to the sum of x-ray irradiation and NC dark activity. CONCLUSION: These novel NCs promise to reduce doses in radiotherapy, helping to reduce unwanted side effects.

Hybrid Polyelectrolyte/Fe3O4 Nanocapsules for Hyperthermia Applications.

We validated here the applicability to hyperthermia treatment of magnetic nanocapsules prepared by the sequential layer-by-layer adsorption of polyelectrolytes and magnetic, Fe3O4 nanoparticles. For the shell preparation around a nanodroplet liquid core, biocompatible polyelectrolytes were used: poly l-lysine as the polycation and poly glutamic acid as the polyanion. The hyperthermia effect was demonstrated by applying the radio frequency (rf) magnetic field with maximum fields H up to 0.025 T and frequencies up to 430 kHz; we found sizable heating effects, with a heating rate up to 0.46 °C/min. We also found effects of irradiation on capsules' morphology that indicated their disruption, thus suggesting their potential use as nanocarriers of drugs that can be locally released on demand. Therefore, these magnetically responsive nanocapsules could be a promising platform for multifunctional biomedical applications such as the controlled release of pharmaceuticals in combination with hyperthermia treatment.

CeF3-ZnO scintillating nanocomposite for self-lighted photodynamic therapy of cancer.

We report on the synthesis and characterization of a composite nanostructure based on the coupling of cerium fluoride (CeF3) and zinc oxide (ZnO) for applications in self-lighted photodynamic therapy. Self-lighted photodynamic therapy is a novel approach for the treatment of deep cancers by low doses of X-rays. CeF3 is an efficient scintillator: when illuminated by X-rays it emits UV light by fluorescence at 325 nm. In this work, we simulate this effect by exciting directly CeF3 fluorescence by UV radiation. ZnO is photo-activated in cascade, to produce reactive oxygen species. This effect was recently demonstrated in a physical mixture of distinct nanoparticles of CeF3 and ZnO [Radiat. Meas. (2013) 59:139-143]. Oxide surface provides a platform for rational functionalization, e.g., by targeting molecules for specific tumors. Our composite nanostructure is stable in aqueous media with excellent optical coupling between the two components; we characterize its uptake and its good cell viability, with very low intrinsic cytotoxicity in dark.

Hydrophobic Silica Nanoparticles Induce Gel Phases in Phospholipid Monolayers.

Silica nanoparticles (SiNP) can be incorporated in phospholipid layers to form hybrid organic-inorganic bidimensional mesostructures. Controlling the dynamics in these mesostructures paves the way to high-performance drug-delivery systems. Depending on the different hydrophobicity/hydrophilicity of SiNP, recent X-ray reflectivity experiments have demonstrated opposite structural effects. While these are reasonably well understood, less is known about the effects on the dynamics, which in turn determine molecular diffusivity and the possibility of drug release. In this work we characterize the dynamics of a mixed Langmuir layer made of phospholipid and hydrophobic SiNP. We combine X-ray photon correlation spectroscopy and epifluorescence discrete Fourier microscopy to cover more than 2 decades of Q-range (0.3-80 µm(-1)). We obtain evidence for the onset of an arrested state characterized by intermittent stress-relaxation rearrangement events, corresponding to a gel dominated by attractive interactions. We compare this with our previous results from phospholipid/hydrophilic SiNP films, which show an arrested glassy phase of repulsive disks.

2D dynamical arrest transition in a mixed nanoparticle-phospholipid layer studied in real and momentum spaces.

We investigate the interfacial dynamics of a 2D self-organized mixed layer made of silica nanoparticles interacting with phospholipid (DPPC) monolayers at the air/water interface. This system has biological relevance, allowing investigation of toxicological effects of nanoparticles on model membranes and lung surfactants. It might also provide bio-inspired technological solutions, exploiting the self-organization of DPPC to produce a non-trivial 2D structuration of nanoparticles. The characterization of interfacial dynamics yields information on the effects of NPs on the mechanical properties, important to improve performances of systems such as colloidosomes, foams, creams. For this, we combine micro-tracking in real-space with measurement in momentum-space via x-ray photon-correlation spectroscopy and Digital Fourier Microscopy. Using these complementary techniques, we extend the spatial range of investigation beyond the limits of each one. We find a dynamical transition from Brownian diffusion to an arrested state driven by compression, characterized by intermittent rearrangements, compatible with a repulsive glass phase. The rearrangement and relaxation of the monolayer structure results dramatically hindered by the presence of NPs, which is relevant to explain some the mechanical features observed for the dynamic surface pressure response of these systems and which can be relevant for the respiratory physiology and for future drug-delivery composite systems.