RESUMEN
Within the last decade, technological advances have led to amazing genetic insights into Mendelian and multifactorial ocular diseases. We provide a perspective of the progress in gene discovery and discuss the implications. We believe that the time has come to redefine the goals and begin utilizing the genetic knowledge for clinical management and treatment design. The unbelievable opportunities now exist for those nimble enough to seize them.
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Oftalmopatías/genética , Estudios de Asociación Genética/tendencias , Oftalmopatías/etiología , Oftalmopatías/terapia , Predisposición Genética a la Enfermedad , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
Gene mutations that encode retinoschisin (RS1) cause X-linked retinoschisis (XLRS), a form of juvenile macular and retinal degeneration that affects males. RS1 is an adhesive protein which is proposed to preserve the structural and functional integrity of the retina, but there is very little evidence of the mechanism by which protein changes are related to XLRS disease. Here, we report molecular modeling of the RS1 protein and consider perturbations caused by mutations found in human XLRS subjects. In 60 XLRS patients who share 27 missense mutations, we then evaluated possible correlations of the molecular modeling with retinal function as determined by the electroretinogram (ERG) a- and b-waves. The b/a-wave ratio reflects visual-signal transfer in retina. We sorted the ERG b/a-ratios by patient age and by the mutation impact on protein structure. The majority of RS1 mutations caused minimal structure perturbation and targeted the protein surface. These patients' b/a-ratios were similar across younger and older subjects. Maximum structural perturbations from either the removal or insertion of cysteine residues or changes in the hydrophobic core were associated with greater difference in the b/a-ratio with age, with a significantly smaller ratio at younger ages, analogous to the ERG changes with age observed in mice with no RS1-protein expression due to a recombinant RS1-knockout gene. The molecular modeling suggests an association between the predicted structural alteration and/or damage to retinoschisin and the severity of XLRS as measured by the ERG analogous to the RS1-knockout mouse.
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Proteínas del Ojo/genética , Modelos Moleculares , Mutación , Retinosquisis/genética , Adolescente , Adulto , Factores de Edad , Anciano , Niño , Cisteína/química , Electrorretinografía , Proteínas del Ojo/química , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Estructura Molecular , FenotipoRESUMEN
PURPOSE: To document the progression of disease in male and female members of a previously described family with X-linked dominant retinitis pigmentosa (RP) caused by a de novo insertion after nucleotide 173 in exon ORF15 of RPGR. METHODS: The clinical records of 19 members of family UTAD054 were reviewed. Their evaluations consisted of confirmation of family history, standardised electroretinograms (ERGs), Goldmann visual fields, and periodic ophthalmological examinations over a 23-year period. RESULTS: Male members of family UTAD054 had non-recordable to barely recordable ERGs from early childhood. The males showed contracted central fields and developed more severe retinopathy than the females. The female members showed a disease onset delayed to teenage years, recordable but diminishing photopic and scotopic ERG amplitudes in a cone-rod pattern, progressive loss and often asymmetric visual fields, and diffuse atrophic retinopathy with fewer pigment deposits compared with males. CONCLUSIONS: This insertion mutation in the RPGR exon ORF15 is associated with a RP phenotype that severely affects males early and females by 30 years of age, and is highly penetrant in female members. Families with dominant-acting RPGR mutations may be mistaken to have an autosomal mode of inheritance resulting in an incorrect prediction of recurrence risk and prognosis. Broader recognition of X-linked RP forms with dominant inheritance is necessary to facilitate appropriate counselling of these patients.
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Proteínas del Ojo/genética , Enfermedades Genéticas Ligadas al Cromosoma X/genética , Mutagénesis Insercional/genética , Retinitis Pigmentosa/genética , Adolescente , Adulto , Edad de Inicio , Niño , Preescolar , Adaptación a la Oscuridad/fisiología , Progresión de la Enfermedad , Electrorretinografía , Exones/genética , Femenino , Estudios de Seguimiento , Enfermedades Genéticas Ligadas al Cromosoma X/fisiopatología , Humanos , Lactante , Masculino , Persona de Mediana Edad , Errores de Refracción , Retinitis Pigmentosa/fisiopatología , Umbral Sensorial , Agudeza Visual , Campos Visuales/fisiología , Adulto JovenRESUMEN
X-linked juvenile retinoschisis (XLRS) is a neurodevelopmental abnormality caused by retinoschisin gene mutations. XLRS is characterized by splitting through the retinal layers and impaired synaptic transmission of visual signals resulting in impaired acuity and a propensity to retinal detachment. Several groups have treated murine retinoschisis models successfully using adeno-associated virus (AAV) vectors. Owing to the fragile nature of XLRS retina, translating this therapy to the clinic may require an alternative to invasive subretinal vector administration. Here we show that all layers of the retinoschisin knockout (Rs1-KO) mouse retina can be transduced efficiently with AAV vectors administered by simple vitreous injection. Retinoschisin expression was restricted to the neuroretina using a new vector that uses a 3.5-kb human retinoschisin promoter and an AAV type 8 capsid. Intravitreal administration to Rs1-KO mice resulted in robust retinoschisin expression with a retinal distribution similar to that observed in wild-type retina, including the expression by the photoreceptors lying deep in the retina. No off-target expression was observed. Rs1-KO mice treated with this vector showed a decrease in the schisis cavities and had improved retinal signaling evaluated by recording the electroretinogram 11-15 weeks after the application.
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Dependovirus/genética , Proteínas del Ojo/genética , Técnicas de Transferencia de Gen , Vectores Genéticos/genética , Retina/metabolismo , Retinosquisis/terapia , Animales , Modelos Animales de Enfermedad , Electrorretinografía , Técnica del Anticuerpo Fluorescente , Expresión Génica , Terapia Genética/métodos , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Inyecciones Intraoculares , Masculino , Ratones , Ratones Noqueados , Regiones Promotoras Genéticas , Retina/patología , Retinosquisis/genética , Retinosquisis/patología , TransfecciónRESUMEN
Thirteen retinoschisis males with genotyped XLRS1 gene mutations were examined by electroretinogram (ERG) techniques to determine photoreceptor involvement and ON-pathway and OFF-pathway sites of dysfunction. Parameters R(max) and logS determined by fitting the mathematical model of the activation phase of phototransduction to the scotopic and photopic a-wave responses, were not significantly different from normal. However, the XLRS photopic a-wave amplitudes were significantly lower than normal across all intensities, consistent with defective signaling in the OFF pathway. Long flash (150 ms) ON-OFF photopic responses showed reduced b-wave amplitude but normal d-wave amplitude, giving a reduced b/d ratio of <1.32 Hz photopic flicker ERG fundamental frequency responses showed reduced amplitude and delayed phase, consistent with abnormal signaling by both the ON- and OFF-pathway components. These results indicate that the XLRS1 protein appears not to affect photoreceptor function directly for most XLRS males, and that ERG signaling abnormalities occur in both the ON- and OFF-pathway components that originate in the proximal retina.
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Células Fotorreceptoras de Vertebrados/fisiología , Degeneración Retiniana/fisiopatología , Adolescente , Adulto , Análisis de Varianza , Estudios de Casos y Controles , Niño , Percepción de Color/fisiología , Electrorretinografía/métodos , Ligamiento Genético , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Modelos Neurológicos , Mutación/genética , Psicofísica , Degeneración Retiniana/genética , Visión Ocular/fisiología , Cromosoma XAsunto(s)
Proteínas del Ojo/genética , Mutación Puntual , Degeneración Retiniana/genética , Familia , Femenino , Angiografía con Fluoresceína , Fondo de Ojo , Ligamiento Genético , Humanos , Lactante , Masculino , Persona de Mediana Edad , Fenotipo , Degeneración Retiniana/diagnóstico , Agudeza Visual , Cromosoma XRESUMEN
The protein neural retina leucine zipper (Nrl) is a basic motif-leucine zipper transcription factor that is preferentially expressed in rod photoreceptors. It acts synergistically with Crx to regulate rhodopsin transcription. Missense mutations in human NRL have been associated with autosomal dominant retinitis pigmentosa. Here we report that deletion of Nrl in mice results in the complete loss of rod function and super-normal cone function, mediated by S cones. The photoreceptors in the Nrl-/- retina have cone-like nuclear morphology and short, sparse outer segments with abnormal disks. Analysis of retinal gene expression confirms the apparent functional transformation of rods into S cones in the Nrl-/- retina. On the basis of these findings, we postulate that Nrl acts as a 'molecular switch' during rod-cell development by directly modulating rod-specific genes while simultaneously inhibiting the S-cone pathway through the activation of Nr2e3.
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Proteínas de Unión al ADN/fisiología , Proteínas del Ojo/fisiología , Células Fotorreceptoras Retinianas Bastones/crecimiento & desarrollo , Factores de Transcripción/fisiología , Animales , Secuencia de Bases , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico , Cartilla de ADN , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Inmunohistoquímica , Leucina Zippers , Ratones , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
To explore the possible influence of defined genetic backgrounds on photoreceptor viability and function in mice carrying a targeted disruption of the rhodopsin gene, the severities of retinopathies in Rho-/- mice on C57BL/6J and 129Sv congenic backgrounds were compared by light microscopy and electroretinography and qualitatively by in situ end labeling of DNA in apoptotic photoreceptor nuclei of retinal sections. Cone photoreceptor viability and function were shown to deteriorate more slowly on the C57BL/6J background in comparison to that of the 129Sv, with significantly greater numbers of outer nuclear layer nuclei in the retinas of C57BL/6J mice at 3 and 4 months of age. Both amplitude and waveform features of the ERG were shown to be remarkably different in the two strains, indicating an approximately 6-fold difference in C57BL/6J Rho-/- mice compared to 129Sv Rho-/- mice at 80 days. Thus, in comparison with the 129Sv strain, genetic modifiers appear to constitute a component of the C57BL/6J background, the expression of which significantly protects cone photoreceptors from apoptotic death in a mutation-induced murine retinopathy. The differences in phenotype revealed in this study are sufficient in principle to provide a basis for comparisons to be made between QTLs in light-induced and mutation-induced systems.
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Ratones Endogámicos/genética , Células Fotorreceptoras de Vertebrados/fisiología , Células Fotorreceptoras de Vertebrados/ultraestructura , Rodopsina/deficiencia , Envejecimiento/fisiología , Animales , Núcleo Celular/ultraestructura , Supervivencia Celular , Electrorretinografía , Ratones , Ratones Endogámicos C57BL/genética , Ratones Noqueados/genética , Degeneración Nerviosa/patología , Fenotipo , Retina/ultraestructura , Células Fotorreceptoras Retinianas Conos/fisiología , Rodopsina/genética , Especificidad de la EspecieRESUMEN
The a-wave of the human dark-adapted ERG is thought to derive from activity of rod photoreceptors. However, other sources within the retina could potentially perturb this simple equation. We investigated the extent to which the short-latency dark-adapted rod a-wave of the primate ERG is dominated by the rod photoresponse and the applicability of the phototransduction model to fit the rod a-wave. Dark-adapted Ganzfeld ERGs were elicited over a 5-log-unit intensity range using short bright xenon flashes, and the light-adapted cone responses were subtracted to isolate the rod ERG a-wave. Intravitreal 4-phosphono-butyric acid (APB) and cis-2,3-piperidine-dicarboxylic acid (PDA) were applied to isolate the photoreceptor response. The Hood and Birch version of the phototransduction model, Rmax[1 - e(-I x S x (t-t(eff)))2], was fitted to the a-wave data while allowing Rmax and S to vary. Three principle observations were made: (1) At flash intensities > or =0.77 log sc-td-s the leading edge of the normalized rod ERG a-wave tracks the isolated photoreceptor response across the first 20 ms or up to the point of b-wave intrusion. The rod ERG a-wave was essentially identical to the isolated receptor response for all intensities that produce peak responses within 14 ms after the flash. (2) The best fit of sensitivity (S) was not affected by APB and/or PDA, suggesting that the inner retina contributes very little to the dark-adapted a-wave. (3) APB always reduced the maximum dark-adapted a-wave amplitude (by 15-30%), and PDA always increased it (by 7-15%). Using the phototransduction model, both events can be interpreted as a scaling of the photoreceptor dark current. This suggests that activity of postreceptor cells somehow influences the rod dark current, possibly by feedback through horizontal cells (although currently not demonstrated for the rod system), or by altering the ionic concentrations near the photoreceptors, or by neuromodulator effects mediated by dopamine or melatonin.
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Adaptación a la Oscuridad/fisiología , Electrorretinografía , Luz , Macaca/fisiología , Células Fotorreceptoras Retinianas Bastones/efectos de la radiación , Aminobutiratos/farmacología , Animales , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Macaca fascicularis , Modelos Biológicos , Estimulación Luminosa/métodos , Ácidos Pipecólicos/farmacología , Células Fotorreceptoras Retinianas Bastones/efectos de los fármacos , Estereoisomerismo , Visión OcularRESUMEN
A dominant form of human congenital nightblindness is caused by a gly90-->asp (G90D) mutation in rhodopsin. G90D has been shown to activate the phototransduction cascade in the absence of light in vitro. Such constitutive activity of G90D rhodopsin in vivo would desensitize rod photoreceptors and lead to nightblindness. In contrast, other rhodopsin mutations typically give rise to nightblindness by causing rod cell death. Thus, the proposed desensitization without rod degeneration would be a novel mechanism for this disorder. To explore this possibility, we induced mice to express G90D opsin in their rods and then examined rod function and morphology, after first crossing the transgenic animals with rhodopsin knock-out mice to obtain appropriate levels of opsin expression. The G90D mouse opsin bound the chromophore and formed a bleachable visual pigment with lambda(max) of 492 nm that supported rod photoresponses. (G+/-, R+/-) retinas, heterozygous for both G90D and wild-type (WT) rhodopsin, possessed normal numbers of photoreceptors and had a normal rhodopsin complement but exhibited considerable loss of rod sensitivity as measured electroretinographically. The rod photoresponses were desensitized, and the response time to peak was faster than in (R+/-) animals. An equivalent desensitization resulted by exposing WT retinas to a background light producing 82 photoisomerizations rod(-1) sec(-1), suggesting that G90D rods in darkness act as if they are partially "light-adapted." Adding a second G90D allele gave (G+/+, R+/-) animals that exhibited a further increase of equivalent background light level but had no rod cell loss by 24 weeks of age. (G+/+, R-/-) retinas that express only the mutant rhodopsin develop normal rod outer segments and show minimal rod cell loss even at 1 year of age. We conclude that G90D is constitutively active in mouse rods in vivo but that it does not cause significant rod degeneration. Instead, G90D desensitizes rods by a process equivalent to light adaptation.
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Adaptación Ocular/genética , Ceguera Nocturna/etiología , Ceguera Nocturna/fisiopatología , Células Fotorreceptoras Retinianas Bastones/fisiopatología , Rodopsina/metabolismo , Alelos , Sustitución de Aminoácidos , Animales , Recuento de Células , Modelos Animales de Enfermedad , Relación Dosis-Respuesta en la Radiación , Electrorretinografía , Genes Dominantes , Genotipo , Heterocigoto , Homocigoto , Humanos , Inmunohistoquímica , Luz , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Retina/patología , Retina/fisiopatología , Células Fotorreceptoras Retinianas Bastones/patología , Rodopsina/genética , Rodopsina/efectos de la radiaciónAsunto(s)
Braquiterapia , Neoplasias de la Coroides/radioterapia , Radioisótopos de Yodo/uso terapéutico , Melanoma/radioterapia , Neoplasias de la Coroides/diagnóstico , Neoplasias de la Coroides/mortalidad , Enucleación del Ojo , Humanos , Melanoma/diagnóstico , Melanoma/mortalidad , Evaluación de Resultado en la Atención de Salud , Ensayos Clínicos Controlados Aleatorios como Asunto , Factores de Riesgo , Tasa de SupervivenciaRESUMEN
The chromophore of the visual pigments, 11-cis retinal, is derived from vitamin A (all-trans retinol) through a series of reactions that take place in retinal pigment epithelium (RPE); (ref. 1). The first of these reactions is catalyzed by lecithin retinol acyltransferase (LRAT); (ref. 2). We screened 267 retinal dystrophy patients for mutations in LRAT and identified disease-associated mutations (S175R and 396delAA) in three individuals with severe, early-onset disease. We showed that the S175R mutant has no acyltransferase activity in transfected COS-7 cells. Our findings highlight the importance of genetic defects in vitamin A metabolism as causes of retinal dystrophies and extend prospects for retinoid replacement therapy in this group of diseases.
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Aciltransferasas/genética , Mutación , Degeneración Retiniana/genética , Aciltransferasas/metabolismo , Edad de Inicio , Animales , Células COS , Membrana Celular/metabolismo , Femenino , Genes Recesivos , Humanos , Masculino , Repeticiones de Microsatélite , Mutación Puntual , Polimorfismo Conformacional Retorcido-SimpleRESUMEN
PURPOSE: To investigate possible protective effects of lens epithelium-derived growth factor (LEDGF) against photoreceptor death in light-damaged, Royal College of Surgeons (RCS) and P23H rhodopsin transgenic rats. METHODS: Twelve-week-old Sprague-Dawley (SD), 6-week-old RCS, and 10-day-old P23H (line 1, heterozygote) rats received an intravitreal injection of LEDGF fused with glutathione-S-transferase (GST-LEDGF). Fellow eyes received vehicle and served as control specimens. Two days after the injections, the SD rats were exposed to light of 2000 lux for 48 hours. Corneal Ganzfeld ERGs were recorded 10 days after light damage, at 10 weeks of age in RCS rats, and at 4 weeks of age in P23H rats. The eyes were then processed for histologic analysis. Heat shock protein (hsp) content in the sensory retina was analyzed quantitatively by protein immunoblot. RESULTS: In light-damaged rats, the ERG indicated retinal protection in GST-LEDGF-injected eyes, with b-wave and STR thresholds being 1.14 +/- 0.50 (mean +/- SD) and 0.60 +/- 0.26 log candela (cd)/m2 lower, respectively, than in vehicle-injected eyes (P < 0.01). The GST-LEDGF-treated eyes had maximum b-wave amplitudes that were significantly larger (P < 0.0005), had more than twice as many remaining photoreceptors, and had better organized outer segments than the control eyes. In RCS rats, the treated eyes had 2.76 +/- 0.73 and 0.83 +/- 0.09 log cd/m(2) lower thresholds for the b-wave and STR, respectively (P < 0.005), and had significantly larger maximum b-wave amplitude (P < 0.0005). GST-LEDGF-treated eyes of RCS rats also had more photoreceptors remaining (P < 0.005) and a thinner debris layer than control eyes. In P23H rats, GST-LEDGF treatment did not protect either retinal function or structure. The retinas from GST-LEDGF-treated eyes of SD and RCS rats had higher levels of hsp25 and alphaB-crystallin than vehicle-injected eyes. CONCLUSIONS: GST-LEDGF protects photoreceptor structure and function in both light-damaged and RCS rats. The increased expression of hsp25 and alphaB-crystallin may play a role in this protection. The absence of rescue in P23H raises the possibility that some forms of inherited retinal degeneration may not be amenable to treatment by intraocular injection of LEDGF.
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Sustancias de Crecimiento/farmacología , Péptidos y Proteínas de Señalización Intercelular , Luz/efectos adversos , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Traumatismos Experimentales por Radiación/prevención & control , Degeneración Retiniana/prevención & control , Animales , Animales Modificados Genéticamente , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/fisiología , Supervivencia Celular/efectos de la radiación , Electrorretinografía , Proteínas de Choque Térmico/metabolismo , Immunoblotting , Inyecciones , Masculino , Células Fotorreceptoras de Vertebrados/fisiología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Traumatismos Experimentales por Radiación/etiología , Traumatismos Experimentales por Radiación/fisiopatología , Ratas , Ratas Mutantes , Ratas Sprague-Dawley , Proteínas Recombinantes de Fusión , Degeneración Retiniana/etiología , Degeneración Retiniana/fisiopatologíaRESUMEN
Isotretinoin (13-cis retinoic acid) is frequently prescribed for severe acne [Peck, G. L., Olsen, T. G., Yoder, F. W., Strauss, J. S., Downing, D. T., Pandya, M., Butkus, D. & Arnaud-Battandier, J. (1979) N. Engl. J. Med. 300, 329-333] but can impair night vision [Fraunfelder, F. T., LaBraico, J. M. & Meyer, S. M. (1985) Am. J. Ophthalmol. 100, 534-537] shortly after the beginning of therapy [Shulman, S. R. (1989) Am. J. Public Health 79, 1565-1568]. As rod photoreceptors are responsible for night vision, we administered isotretinoin to rats to learn whether night blindness resulted from rod cell death or from rod functional impairment. High-dose isotretinoin was given daily for 2 months and produced systemic toxicity, but this caused no histological loss of rod photoreceptors, and rod-driven electroretinogram amplitudes were normal after prolonged dark adaptation. Additional studies showed, however, that even a single dose of isotretinoin slowed the recovery of rod signaling after exposure to an intense bleaching light, and that rhodopsin regeneration was markedly slowed. When only a single dose was given, rod function recovered to normal within several days. Rods and cones both showed slow recovery from bleach after isotretinoin in rats and in mice. HPLC analysis of ocular retinoids after isotretinoin and an intense bleach showed decreased levels of rhodopsin chromophore, 11-cis retinal, and the accumulation of the biosynthetic intermediates, 11-cis and all-trans retinyl esters. Isotretinoin was also found to protect rat photoreceptors from light-induced damage, suggesting that strategies of altering retinoid cycling may have therapeutic implications for some forms of retinal and macular degeneration.
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Isotretinoína/farmacología , Ceguera Nocturna/fisiopatología , Células Fotorreceptoras Retinianas Bastones/fisiopatología , Visión Ocular/efectos de los fármacos , Animales , Isotretinoína/administración & dosificación , Isotretinoína/uso terapéutico , Luz , Masculino , Ratones , Ratones Endogámicos C57BL , Ceguera Nocturna/inducido químicamente , Ceguera Nocturna/metabolismo , Ratas , Ratas Sprague-Dawley , Células Fotorreceptoras Retinianas Conos/efectos de los fármacos , Células Fotorreceptoras Retinianas Conos/fisiopatología , Células Fotorreceptoras Retinianas Bastones/efectos de los fármacos , Células Fotorreceptoras Retinianas Bastones/metabolismo , Rodopsina/biosíntesisRESUMEN
Stargardt-like macular dystrophy (STGD3, MIM 600110) and autosomal dominant macular dystrophy (adMD) are inherited forms of macular degeneration characterized by decreased visual acuity, macular atrophy and extensive fundus flecks. Genetic mapping data suggest that mutations in a single gene may be responsible for both conditions, already known to bear clinical resemblance. Here we limit the minimum genetic region for STGD3 and adMD to a 0.6-cM interval by recombination breakpoint mapping and identify a single 5-bp deletion within the protein-coding region of a new retinal photoreceptor-specific gene, ELOVL4, in all affected members of STGD3 and adMD families. Bioinformatic analysis of ELOVL4 revealed that it has homology to a group of yeast proteins that function in the biosynthesis of very long chain fatty acids. Our results are therefore the first to implicate the biosynthesis of fatty acids in the pathogenesis of inherited macular degeneration.
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Proteínas del Ojo/genética , Genes Dominantes/genética , Degeneración Macular/genética , Proteínas de la Membrana/genética , Eliminación de Secuencia/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , Cromosomas Humanos Par 6/genética , Clonación Molecular , Análisis Mutacional de ADN , Exones/genética , Proteínas del Ojo/química , Proteínas del Ojo/metabolismo , Femenino , Humanos , Hibridación in Situ , Intrones/genética , Escala de Lod , Macaca mulatta/genética , Degeneración Macular/patología , Masculino , Proteínas de la Membrana/química , Proteínas de la Membrana/metabolismo , Ratones , Datos de Secuencia Molecular , Linaje , ARN Mensajero/análisis , ARN Mensajero/genética , Retina/metabolismo , Retina/patología , Alineación de SecuenciaRESUMEN
PURPOSE: To study how the photoreceptoral and postreceptoral ON- and OFF-components contribute to the photopic sine-wave flicker ERG in the monkey by isolating the components with glutamate analogs. METHODS: Monkey photopic flicker ERGs were elicited with sine wave stimuli (mean luminance, 2.66 log cd/m(2); 80% modulation depth, on a 40 cd/m(2) white background) and were recorded for stimulus frequencies of 4 Hz to 64 Hz, before and after intravitreal injection of DL-2-amino-4-phosphonobutyric acid (APB) and cis-2, 3-piperidinedicarboxylic acid (PDA) that block ON- and OFF-bipolar activity, respectively. The amplitude and phase of the fundamental component were analyzed. RESULTS: The flicker response amplitudes increased after APB, for frequencies of 6 Hz to 32 Hz. The further addition of PDA to isolate the photoreceptor component resulted in a relatively small residual response that decreased monotonically from 4 Hz to 32 Hz. The postsynaptic APB (ON-) and PDA (OFF-) sensitive components were isolated by subtraction and were characterized by amplitude and phase vectors. The ON- and OFF-components were larger than the initial control responses for stimuli of 8 Hz to 40 Hz. These two components had a frequency-dependent phase difference of 160 degrees to 230 degrees; normally, they interfere with each other and reduce their net contribution. The phase difference between ON- and OFF-components was nearly 180 degrees for a 10-Hz stimulus, and the phase cancellation caused a prominent dip in amplitude at this frequency. CONCLUSIONS: These results indicate that postreceptoral ON- and OFF-components contribute substantially to the sine-wave flicker ERG, especially at higher stimulus frequencies. Because of phase cancellation, they mask each other in the net response in a frequency dependent fashion. The photoreceptor contribution is greater than the net postsynaptic component only for frequencies of approximately less than or equal to 10 Hz. These results can be summarized by a vector model that may be useful for interpreting changes resulting from retinal disease.
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Aminobutiratos/farmacología , Electrorretinografía/efectos de los fármacos , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Ácidos Pipecólicos/farmacología , Retina/fisiología , Adaptación Ocular , Animales , Interneuronas/efectos de los fármacos , Luz , Macaca mulatta , Estimulación LuminosaRESUMEN
Stargardt-like macular degeneration (STGD(3)) and autosomal dominant macular degeneration (adMD) share phenotypic characters with atrophic age-related macular degeneration (AMD). Mutations in a photoreceptor cell-specific factor involved in the elongation of very long chain fatty acids (ELOVL(4)) were shown to be associated with STGD(3), adMD, and pattern dystrophy. We screened 778 patients with AMD and 551 age-matched controls to define the role of sequence variants in the ELOVL(4) gene in age-related macular degeneration. We detected three sequence variants in the non-coding region and eight variants in the coding region. No statistically significant association was observed between sequence variants in the ELOVL(4) gene and susceptibility to AMD. However, for the detection of modest effects of multiple alleles in a complex disease, the analysis of larger cohorts of patients may be required.
Asunto(s)
Proteínas del Ojo/genética , Degeneración Macular/genética , Proteínas de la Membrana/genética , Mutación/genética , Polimorfismo de Nucleótido Simple/genética , Factores de Edad , Anciano , Cartilla de ADN/química , Exones , Variación Genética , Humanos , Intrones , Degeneración Macular/fisiopatología , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
PURPOSE: To characterize the spectrum of RPE65 mutations present in 453 patients with retinal dystrophy with an interest in understanding the range of functional deficits attributable to sequence variants in this gene. METHODS: The 14 exons of RPE65 were amplified by polymerase chain reaction (PCR) from patients' DNA and analyzed for sequence changes by single-strand conformation polymorphism (SSCP) and direct sequencing. Haplotype analysis was performed using RPE65 intragenic polymorphisms. Patients were examined clinically and with visual function tests. RESULTS: Twenty-one different disease-associated DNA sequence changes predicting missense or nonsense point mutations, insertions, deletions, and splice site defects in RPE65 were identified in 20 patients in homozygous or compound heterozygous form. In one patient, paternal uniparental isodisomy (UPD) of chromosome 1 resulted in homozygosity for a probable functional null allele. Eight of the disease-associated mutations (Y79H, E95Q, E102X, D167Y, 669delCA, IVS7+4a-->g, G436V, and G528V) and one mutation likely to be associated with disease (IVS6+5g-->a) have not been reported previously. The most commonly occurring sequence variant identified in the patients studied was the IVS1+5g-->a mutation, accounting for 9 of 40 (22.5%) total disease alleles. This splice site mutation, as well as R91W, the most common missense mutation, exists on at least two different genetic backgrounds. The phenotype resulting from RPE65 mutations appears to be relatively uniform and independent of mutation class, suggesting that most missense mutations (15 of 40 disease alleles [37.5%]) result in loss of function. At young ages, this group of patients has somewhat better subjective visual capacity than is typically associated with Leber congenital amaurosis (LCA) type I, with a number of patients retaining some useful visual function beyond the second decade of life. CONCLUSIONS: RPE65 mutations account for a significant percentage (11.4%) of disease alleles in patients with early-onset retinal degeneration. The identification and characterization of patients with RPE65 mutations is likely to represent an important resource for future trials of rational therapies for retinal degeneration.
Asunto(s)
Proteínas del Ojo/genética , Mutación Missense , Epitelio Pigmentado Ocular/patología , Proteínas/genética , Degeneración Retiniana/genética , Adolescente , Adulto , Edad de Inicio , Proteínas Portadoras , Niño , Análisis Mutacional de ADN , Electrorretinografía , Haplotipos , Humanos , Persona de Mediana Edad , Fenotipo , Reacción en Cadena de la Polimerasa , Polimorfismo Conformacional Retorcido-Simple , Prevalencia , Retina/fisiología , Degeneración Retiniana/epidemiología , Degeneración Retiniana/patología , Análisis de Secuencia de ADN , Agudeza Visual , cis-trans-IsomerasasRESUMEN
X-linked forms of retinitis pigmentosa (XLRP) are among the most severe, because of their early onset, often leading to significant vision loss before the 4th decade. Previously, the RP15 locus was assigned to Xp22, by linkage analysis of a single pedigree with "X-linked dominant cone-rod degeneration." After clinical reevaluation of a female in this pedigree identified her as affected, we remapped the disease to a 19.5-cM interval (DXS1219-DXS993) at Xp11.4-p21.1. This new interval overlapped both RP3 (RPGR) and COD1. Sequencing of the previously published exons of RPGR revealed no mutations, but a de novo insertion was detected in the new RPGR exon, ORF15. The identification of an RPGR mutation in a family with a severe form of cone and rod degeneration suggests that RPGR mutations may encompass a broader phenotypic spectrum than has previously been recognized in "typical" retinitis pigmentosa.
Asunto(s)
Exones/genética , Ligamiento Genético/genética , Mutación/genética , Sistemas de Lectura Abierta/genética , Retinitis Pigmentosa/genética , Cromosoma X/genética , Adulto , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Femenino , Haplotipos/genética , Humanos , Masculino , Datos de Secuencia Molecular , Linaje , Fenotipo , Recombinación Genética/genéticaRESUMEN
PURPOSE: To correlate retinal functional changes with structural changes in P23H rhodopsin transgenic rats as a model of autosomal dominant retinitis pigmentosa. METHODS: P23H heterozygote (lines 1 and 3) and Sprague-Dawley control rats were studied at 4 to 29 weeks by retinal histology, electroretinogram (ERG), and a-wave transduction modeling. RESULTS: Both line 1 (faster degeneration) and line 3 (slower degeneration) showed progressive rod outer segment (ROS) shortening and outer nuclear layer (ONL) cell loss with age. ERG b-wave maximum amplitude (Vb(max)) decreased with age, but b-wave threshold remained constant within each line despite progressive ONL thinning and ROS shortening. The only exception was in line 1 at 29 weeks, which showed a slight threshold change relative to earlier ages. Va(max) and a-wave threshold changed more rapidly and were more sensitive than the b-wave in reflecting histologic degeneration. Va(max) was linearly proportional to the product of (ROS x ONL) across a two log unit range of data combined from both lines. The photopic b-wave was normal for both lines until the ONL thinned beyond 50%. Phototransduction sensitivity was normal for both lines, and dark-adaptation recovery after bleaching rhodopsin was normal. CONCLUSIONS: The P23H transgenic rat has a slow rod degeneration with initially normal cone function, consistent with clinical findings of P23H patients. However, the normal bleach recovery and the normal phototransduction sensitivity in this rat model are different from human P23H disease. a-Wave measures were more sensitive than the b-wave for tracking changes. b-Wave threshold was inexplicably poor for tracking degeneration. Although line 1 degenerated faster than line 3, the functional-structural correlates were the same. The tight linear relationship between saturated a-wave amplitude and the product of (ROS x ONL) indicates that the density of cGMP-gated channels per unit ROS plasma membrane area remains constant over a wide range of degenerations.