[Content, Distribution, Source Analysis, and Risk Assessment of PAHs in Arable Soils of Taiyuan].

PAHs (polycyclic aromatic hydrocarbons) accumulated in arable soils have significant impacts on farmland quality and human health, which has attracted wide attention from scientists and the public. A total of 22 arable soil samples were collected from Taiyuan, an old industrial city, including three districts (industrial zones, hilly areas, and sewage irrigation area), and the contents of 21 PAHs were detected using the GC-MS method. The sources of PAHs in soils were analyzed using the diagnostic ratios (DRs) method and positive matrix factorization (PMF) model, and the soil health risks were analyzed using the incremental lifetime cancer risk (ILCR) model. The results indicated that the average concentrations of Σ21PAHs and Σ16PAHs in arable soils of Taiyuan were 934.6 ng·g-1 and 787.7 ng·g-1, respectively, which were lower than the soil pollution risk screening value of agricultural land stipulated in GB 15168-2018. 3-5 rings PAHs were the dominant components, accounting for~90% of the Σ21PAHs. Approximately 60% of sites in industrial zones, 13% in hilly areas, and 33% in the sewage irrigation area had high PAHs contents larger than 1000 ng·g-1. The spatial distribution of PAHs showed that more severe PAHs pollution in the soil occurred in industrial areas than that in the other two districts. The DRs suggested that the combustion of coals, bio-masses, and traffic emissions were the dominant sources for PAHs pollution in arable soils in Taiyuan. The simulation results of the PMF model indicated that the sources and contribution rates of PAHs in cultivated soils were coal and biomass burning sources (59%), traffic sources (22%), and coking sources (19%). The risk assessment confirmed that the arable soils in Taiyuan had high potential carcinogenic risks; thus, more attention should be paid to the PAHs pollutions in arable soils.

Influence of leaf morphological characteristics on the dynamic changes of particulate matter retention and grain size distributions.

ABSTRACTPlant leaves can retain atmospheric particulate matter (PM) and thus alleviate air pollution. Herein, four plant species (Cerasus. serrulata, H. syriacus, H. tuberosus, and E. japonicus) from the central part of Shanxi Province, China, were investigated to analyse the dynamic changes in their PM retention capacity and grain size over five periods. The relationship between leaf morphological characteristics (leaf size and leaf surface microstructure) and dynamic changes were discussed in PM retention, revealing the influence of leaf morphological characteristics on the amount of PM retention and its composition. The results showed that amount and grain sizes of the retained PM differed significantly among the various studied species; however, the trends in PM retention of different species in the time series were the same. The grain size distributions of PM from the four species displayed a bimodal distribution, in which the main peak distribution range was 5-60 µm and the secondary peak distribution range was 0.4-1 µm. Leaves of smaller sizes and those with rough surfaces had a high PM retention capacity. Leaves with deep grooves are conductive to retaining PM2.5 and PM2.5-10, while leaves with hair are conductive to retaining PM>10. Therefore, the morphological characteristics of leaves should be considered when selecting the use of plant species to alleviate air pollution.Highlights Dynamics of PM retention capacity and grain size distributions of four plant species were analysed.Grain size distributions of PM retained on leaves had a bimodal distribution.Small leaves with grooves or hair are conductive to PM retention.Grooves are conductive to fine PM retention while hairs are conductive to coarse PM retention.

Can non-swallowing function assessment predict nasogastric tube removal in patients with poststroke dysphagia? A clinical study.

Objective: This study aimed to predict nasogastric tube (NGT) removal in patients with poststroke dysphagia (PSD) by non-swallowing function assessment. Methods: We enrolled 232 eligible patients and performed rehabilitation. The Fugl-Meyer assessment motor (FMM) and National Institute of Health Stroke Scale (NIHSS) scores were used to measure the motor and overall nervous system functions. Predictors for NGT removal in patients with PSD after rehabilitation were analyzed. Results: Of the 232 included patients, the NGTs were removed from 78% of them, while 22% were dependent on a feeding tube after 4 weeks of rehabilitation. Compared to the preserved NGT group, older age, a higher rate of intubation or tracheostomy, and more severe baseline functions were found in the NGT removal group. Age [odds ratio (OR) = 0.907; 95% confidence interval (CI): 0.859-0.957; p = 0.000], difference in the FMM score after 4 weeks of rehabilitation (OR = 1.219; 95% CI: 1.145-1.299; p = 0.00), and item 9 of NIHSS (OR = 0.488; 95% CI: 0.252-0.946; p = 0.034) were predictors of NGT removal after rehabilitation. Conclusion: We established a predictive model in patients with PSD using a non-swallowing assessment, which enabled us to predict swallowing recovery based on the non-swallowing function.

LncRNA STXBP5-AS1 suppresses stem cell-like properties of pancreatic cancer by epigenetically inhibiting neighboring androglobin gene expression.

Previous studies suggest the tumor suppressor role of long non-coding RNA (lncRNA) STXBP5-AS1 in cervical and gastric cancer, but its expression pattern and functional mechanism are still elusive in pancreatic cancer (PC). Relative expression of STXBP5-AS1 in PC both in vivo and in vitro was analyzed by real-time PCR. IC50 of Gemcitabine was determined by the MTT assay. Cell proliferation in response to drug treatment was investigated by colony formation assay. Cell apoptosis was measured by both caspase-3 activity and Annexin V/PI staining. Cell invasion capacity was scored by the transwell assay in vitro, and lung metastasis was examined with the tail vein injection assay. Cell stemness was determined in vitro by sphere formation and marker profiling, respectively, and in vivo by limited dilution of xenograft tumor incidence. Subcellular localization of STXBP5-AS1 was analyzed with fractionation PCR. Association between STXBP5-AS1 and EZH2 was investigated by RNA-immunoprecipitation. The binding of EZH2 on ADGB promoter was analyzed by chromatin immunoprecipitation. The methylation was quantified by bisulfite sequencing. We showed downregulation of STXBP5-AS1 in PC associated with poor prognosis. Ectopic STXBP5-AS1 inhibited chemoresistance and metastasis of PC cells. In addition, STXBP5-AS1 compromised stemness of PC cells. Mechanistically, STXBP5-AS1 potently recruited EZH2 and epigenetically regulated neighboring ADGB transcription, which predominantly mediated the inhibitory effects of STXBP5-AS1 on stem cell-like properties of PC cells. Our study highlights the importance of the STXBP5-EZH2-ADGB axis in chemoresistance and stem cell-like properties of PC.

Anterior gradient 2 is a novel pro-tumor factor in pancreatic cancer under NF-κB subunit RelA trans-regulation that can be suppressed by eugenic acid.

This study aimed to examine eugenic acid (EA) as an alternative therapeutic approach against pancreatic cancer. The pancreatic cancer xenograft mouse model was employed to determine the impacts of treatment with EA on the growth of tumors. Expressions of NF-κB subunit RelA as well as Anterior gradient 2 (AGR2) were quantified in pancreatic cells treated with EA. Chromatin immunoprecipitation and luciferase report assay were performed to examine the regulation of AGR2 by RelA. The function of AGR2 as a downstream effector EA treatment was further assessed through overexpression of AGR2 in pancreatic cells. EA suppressed the growth of xenograft pancreatic tumor, and promoted the overall survival of animals with xenograft tumors. Furthermore, EA downregulated the expression of AGR2 in pancreatic cancer cells via the RelA binding site. Ectopic AGR2 overexpression attenuated the EA-elicited inhibition on the growth of xenograft pancreatic tumor, and negated the EA-induced enhancement of mouse survival. EA ameliorates pancreatic cancer through suppression of AGR2 expression, and future studies in clinical settings are needed to further assess the anti-cancer efficacy of EA.

What Determines Good Rehabilitation Recovery in Stroke Patients with very Severe Disability? A Retrospective Cohort Study.

OBJECTIVE: This study aimed to evaluate predictors of good recovery after rehabilitation in stroke patients with very severe disability. METHODS: 168 Eligible patients were enrolled. Fugl-Meyer of motor score (FMM), National Institute of Health Stroke Scale (NIHSS), and Modified Barthel Index (MBI) were used to measure the motor and degree of disability. Logistic regressions were used to investigate the effect of 21 pre-existing conditions on efficacy outcomes after 4 weeks rehabilitation. RESULTS: The results indicated the following: 1) FMM (r = 0.2, P=0.02) and NIHSS (r = -0.2, P=0.00) were significantly correlated (positively and negatively, respectively) with fat-free mass index (FFMI); 2)FFMI (odds ratio [OR],1.9; 95% CI, 1.1 to 3.3; P=0.02), OAI (OR, 0.9; 95% CI, 0.9 to 1.0; P=0.00), and FMM (OR, 1.0; 95% CI, 1.0 to 1.1; P=0.00) were revealed as predictors of good recovery after 4 weeks rehabilitation; 3) The model could predict the possibility of good recovery in stroke patients with very severe disability with excellent sensitivity and specificity(80.0% and 67.1%, respectively). CONCLUSIONS: The results could enable clinicians to predict good recovery after rehabilitation in patients with very severe post-stroke disability.

A Retrospective Analysis of Factors Impacting Rehabilitation Outcomes in Patients With Spontaneous Intracerebral Hemorrhage.

OBJECTIVE: This study aimed to identify the factors associated with motor and activities of daily living recovery after 4-wk rehabilitation in patients with intracerebral hemorrhage. DESIGN: Eight hundred ten eligible patients were enrolled and engaged in 4-wk rehabilitation. Fugl-Meyer score and Modified Barthel Index were used to measure the motor and activities of daily living function. Multivariate linear regressions were used to investigate the effect of the 23 preexisting conditions in intracerebral hemorrhage patients (eg, scales of functional abilities, spasticity, cognition, and mental disorders) on efficacy outcomes after 4-wk rehabilitation. RESULTS: The results indicated the following: (1) young age, absence of diabetes mellitus, right hemiplegia, early rehabilitation, elbow spasticity, and higher cognitive and motor function were significantly associated with better prognosis after 4-wk rehabilitation; (2) patients who started rehabilitation within 120 days of stroke onset had significantly better outcomes; (3) activities of daily living function could be further improved for those patients wherein the time since stroke onset was more than 121 days; and (4) improving cognitive function may improve functional ability after rehabilitation in patients with intracerebral hemorrhage. CONCLUSIONS: The results could enable clinicians to predict the intracerebral hemorrhage rehabilitation outcome and achieve the maximum favorable outcome to facilitate personal independence.

Synergistic inhibition of lung cancer cells by EGCG and NF-κB inhibitor BAY11-7082.

Lung cancer has a poor 5-year survival rate and is the leading cause of cancer-related deaths worldwide. Thus, the development of more efficient therapeutic strategies is urgently needed. Many studies have shown that EGCG, a major polyphenol found in green tea, has potential anticancer effects. The present study aims to investigate the molecular mechanism of EGCG-mediated inhibition of proliferation in lung cancer cells and to explore the effects of combined treatment with EGCG and an NF-κB inhibitor, BAY11-7082, in A549 and H1299 cells both in vitro and in vivo. Our results showed that EGCG inhibits cell proliferation and migration and induces apoptosis in A549 and H1299 cells at relatively high concentrations (IC50=86.4 µM for A549 cells and 80.6 µM for H1299 cells). These effects are partially achieved via inhibition of the NF-κB signaling pathway. Combined treatment with EGCG and BAY11-7082, a potent NF-κB inhibitor, shows significant synergistic effects at relatively low concentrations. The inhibition rate reached approximately 50% in cells treated for 72 h with 20 µM EGCG and 5 µM (A549 cells) or 2.5 µM BAY11-7082 (H1299 cells). This synergistic anti-tumor effect was also observed in a xenograft model. These results indicated that EGCG inhibits lung cancer cell proliferation by suppressing NF-κB signaling. Coadministration of EGCG and BAY11-7082 has a synergistic effect both in vitro and in vivo and may serve as a novel therapeutic strategy for lung cancer.