Prevalence and Pattern of Ocular Trauma in a Secondary Eye Care Center in Nepal: A Descriptive Cross-sectional Study.

INTRODUCTION: Ocular trauma stands as the leading preventable cause of monocular blindness worldwide. The aim of the study was to calculate the prevalence of ocular trauma and the circumstances, causes, and types of ocular injuries at emergency department of Community Eye Hospital. METHODS: This was a descriptive cross-sectional study conducted retrospectively on patientsvpresenting to the Emergency Department in the year 2020. Ethical clearance was received from the Institutional Review Board with reference number 12/2021. Data collection commenced in April 2021, following the approval in March 2021. Descriptive statistics with mean and frequency were used for analysis. RESULTS: Among the 6526 emergency cases visiting the emergency department of Hetauda community eye hospital the prevalence of ocular trauma was 2143 (32.83%; 95% CI: 31.69%-33.97%). The mean age among the 2143 trauma cases was 33.55±15.63 years. Among them, 1851 (86.40%) fell in the working age group. The male-to-female ratio was 3:1. Occupational injuries due to welding, agriculture and industries were in 604 (28.19%) of all ocular injuries. CONCLUSIONS: The prevalence of ocular trauma in our study was higher than other studies. Occupational ocular trauma mainly welding injury, cement factory injury and agricultural injury are common cause of ocular trauma.

An assessment of the relationship between spring frost indicators and global crop yield losses.

Reports on the influences of spring frost on crop losses are not consistent, which may be because insufficient indicators of spring frost were included in the analysis. To bridge this gap, we analyzed global temperature datasets and production data for the three major crops of maize, winter wheat, and rice from 1981 to 2016. Five indicators of spring frost events: temperature fluctuation (Tv), temperature difference (Td), duration (Thour), occurrence date (Tdate), and frequency (Tnum) were considered to assess their relationship with yield losses. Linear regression was employed to analyze the change trends in five indicators and random forest was utilized to investigate the relationship between yield loss and indicators of spring frost. Our findings reveal that, despite a decline in the number of spring frost events during global warming, not all the five indicators declined over time. Tv is the most important indicator for yield losses in maize and winter wheat, which shows an increasing trend in their growing regions and provides an explanation for the increasing yield losses of maize and winter wheat over time. Td is the most important indicator of for rice yield losses but it shows a decreasing trend in rice-growing areas, which explains why rice yield losses from spring frosts in recent years are not significant.

Rice plants treated with biochar derived from Spirulina (Arthrospira platensis) optimize resource allocation towards seed production.

The use of biofertilizers is becoming an economical and environmentally friendly alternative to promote sustainable agriculture. Biochar from microalgae/cyanobacteria can be applied to enhance the productivity of food crops through soil improvement, slow nutrient absorption and release, increased water uptake, and long-term mitigation of greenhouse gas sequestration. Therefore, the aim of this study was to evaluate the stimulatory effects of biochar produced from Spirulina (Arthrospira platensis) biomass on the development and seed production of rice plants. Biochar was produced by slow pyrolysis at 300°C, and characterization was performed through microscopy, chemical, and structural composition analyses. Molecular and physiological analyses were performed in rice plants submitted to different biochar concentrations (0.02, 0.1, and 0.5 mg mL-1) to assess growth and productivity parameters. Morphological and physicochemical characterization revealed a heterogeneous morphology and the presence of several minerals (Na, K, P, Mg, Ca, S, Fe, and Si) in the biochar composition. Chemical modification of compounds post-pyrolysis and a highly porous structure with micropores were observed. Rice plants submitted to 0.5 mg mL-1 of biochar presented a decrease in root length, followed by an increase in root dry weight. The same concentration influenced seed production, with an increase of 44% in the number of seeds per plant, 17% in the percentage of full seeds per plant, 12% in the weight of 1,000 full seeds, 53% in the seed weight per plant, and 12% in grain area. Differential proteomic analyses in shoots and roots of rice plants submitted to 0.5 mg mL-1 of biochar for 20 days revealed a fine-tuning of resource allocation towards seed production. These results suggest that biochar derived from Arthrospira platensis biomass can stimulate rice seed production.

Development of a bionic hexapod robot with adaptive gait and clearance for enhanced agricultural field scouting.

High agility, maneuverability, and payload capacity, combined with small footprints, make legged robots well-suited for precision agriculture applications. In this study, we introduce a novel bionic hexapod robot designed for agricultural applications to address the limitations of traditional wheeled and aerial robots. The robot features a terrain-adaptive gait and adjustable clearance to ensure stability and robustness over various terrains and obstacles. Equipped with a high-precision Inertial Measurement Unit (IMU), the robot is able to monitor its attitude in real time to maintain balance. To enhance obstacle detection and self-navigation capabilities, we have designed an advanced version of the robot equipped with an optional advanced sensing system. This advanced version includes LiDAR, stereo cameras, and distance sensors to enable obstacle detection and self-navigation capabilities. We have tested the standard version of the robot under different ground conditions, including hard concrete floors, rugged grass, slopes, and uneven field with obstacles. The robot maintains good stability with pitch angle fluctuations ranging from -11.5° to 8.6° in all conditions and can walk on slopes with gradients up to 17°. These trials demonstrated the robot's adaptability to complex field environments and validated its ability to maintain stability and efficiency. In addition, the terrain-adaptive algorithm is more energy efficient than traditional obstacle avoidance algorithms, reducing energy consumption by 14.4% for each obstacle crossed. Combined with its flexible and lightweight design, our robot shows significant potential in improving agricultural practices by increasing efficiency, lowering labor costs, and enhancing sustainability. In our future work, we will further develop the robot's energy efficiency, durability in various environmental conditions, and compatibility with different crops and farming methods.

Forage conservation is a neglected nitrous oxide source.

Agricultural activities are the major anthropogenic source of nitrous oxide ( N 2 O ), an important greenhouse gas and ozone-depleting substance. However, the role of forage conservation as a potential source of N 2 O has rarely been studied. We investigated N 2 O production from the simulated silage of the three major crops-maize, alfalfa, and sorghum-used for silage in the United States, which comprises over 90% of the total silage production. Our findings revealed that a substantial N 2 O could be generated, potentially placing forage conservation as the third largest N 2 O source in the agricultural sector. Notably, the application of chlorate as an additive significantly reduced N 2 O production, but neither acetylene nor intermittent exposure to oxygen showed any impact. Overall, the results highlight that denitrifiers, rather than nitrifiers, are responsible for N 2 O production from silage, which was confirmed by molecular analyses. Our study reveals a previously unexplored source of N 2 O and provides a crucial mechanistic understanding for effective mitigation strategies.

Field evidence for the role of plant volatiles induced by caterpillar oral secretion in prey localization by predatory social wasps.

One assumed function of herbivore-induced plant volatiles (HIPVs) is to attract natural enemies of the inducing herbivores. Field evidence for this is scarce. In addition, the assumption that elicitors in oral secretions that trigger the volatile emissions are essential for the attraction of natural enemies has not yet been demonstrated under field conditions. After observing predatory social wasps removing caterpillars from maize plants, we hypothesized that these wasps use HIPVs to locate their prey. To test this, we conducted an experiment that simultaneously explored the importance of caterpillar oral secretions in the interaction. Spodoptera caterpillars pinned onto mechanically damaged plants treated with oral secretion were more likely to be attacked by wasps compared with caterpillars on plants that were only mechanically wounded. Both of the latter treatments were considerably more attractive than plants only treated with oral secretion or left untreated. Subsequent analyses of headspace volatiles confirmed differences in emitted volatiles that likely account for the differential predation across treatments. These findings highlight the importance of HIPVs in prey localization by social wasps, hitherto underappreciated potential biocontrol agents and provide evidence for the role that elicitors play in inducing attractive odour blends.

Occupational respiratory disease in Eastern Slovakia between 1990-2021: a shift from agriculture to industrial manufacturing.

OBJECTIVES: Occupational allergic respiratory diseases frequently occur in individuals working in the agricultural and food production sectors, textile manufacturing, and industries involving exposure to isocyanates. The study aimed to describe trends surrounding the prevalence of occupational asthma (OA), occupational rhinitis (OR), and occupational hypersensitivity pneumonitis (OHP) in Eastern Slovakia between 1990-2021. METHODS: All cases of OA, OR, and OHP registered in a database at the Louis Pasteur University Hospital in Kosice, Slovakia, between 1990 and 2021, were divided into categories based on economic sector (agricultural, food production sectors, textile manufacturing, healthcare, industrial manufacturing, and tertiary sector) and causal agent. Changes in disease prevalence, causal agents, and economic sector association over time were analysed. RESULTS: There were 287 occupational respiratory cases (179 OA, 65 OR, and 43 OHP cases). The annual prevalence of OA declined significantly over the study period (p < 0.05). Overall, there was a significant decrease in cases from the agricultural (p < 0.001) and an increase in the industrial manufacturing (p < 0.01). The number of cases due to farming agents fell markedly over the study period, while metalworking fluids (MWFs) were found to be the most common causes of allergic respiratory diseases since 2018. CONCLUSIONS: This study found a decrease in the number of OA cases, as well as changes in economic sectors and causal agents associated with OA and OHP, specifically, in the agricultural sector, with MWFs from the industrial manufacturing sector now being the most common aetiological agent.

Core Principles and Practices for the Design, Implementation, and Evaluation of Social and Behavior Change for Nutrition in Low- and Middle-Income Contexts with Special Applications for Nutrition-Sensitive Agriculture.

Background: There is currently no cogent set of standards to guide the design, implementation and evaluation of nutrition social and behavior change (SBC), including for nutrition-sensitive agriculture (NSA). Objectives: We aimed to capture, consolidate, and describe SBC core principles and practices (CPPs), reflecting professional consensus, and to offer programmatic examples that illustrate their application for NSA projects in low- and middle-income countries. Methods: We conducted a narrative review following a 4-step iterative process to identify and describe SBC CPPs. We first reviewed general SBC frameworks and technical documents and developed a preliminary list of CPPs and their definitions. Following review and feedback from 8 content experts, we revised the CPPs, incorporating the panel's feedback, and conducted a more specific search of the peer-reviewed and gray literature. We presented a revised draft of the CPPs to 26 NSA researchers, practitioners, and implementers at the 2022 Agriculture, Nutrition and Health Academy annual conference. We then conducted a focused review of each CPP, and 3 content experts rereviewed the final draft. Results: We reviewed ∼475 documents and resources resulting in a set of 4 core principles: 1) following a systematic, strategic method in designing, implementing, and evaluating SBC activities; 2) ensuring design and implementation are evidence-based; 3) grounding design and implementation in theory; and 4) authentically engaging communities. Additionally, we identified 11 core practices and mapped these to the different stages in the SBC design, implementation, and evaluation cycle. Detailed descriptions, illustrative examples and resources for implementation are provided for each CPP. Conclusions: An explicit set of CPPs for SBC can serve as a guide for design, research, implementation, and evaluation of nutrition and NSA programs; help standardize knowledge sharing and production; and contribute to improved quality of implementation. Broader consultation with SBC practitioners and researchers will further consensus on this work.

Effectiveness of Elements of Social Behavior Change Activities in Nutrition-Sensitive Agriculture Programs: A Systematic Review.

Background: Limited research exists on the specific approaches and behavior change techniques (BCT) used in nutrition-sensitive agriculture (NSA) programs and their effects on diet diversity. Objectives: We aimed to describe nutrition-related social behavior change (SBC) in the context of NSA and quantify the effectiveness of different SBC components of NSA programs in improving diet diversity. Methods: We searched PubMed, Embase, Web of Science, the International Food Policy and Research Institute repository, and Agricola for articles published between 2000 and 2023. We identified the agricultural activities each project used as a pathway to improved nutrition (ag-nutrition pathways), identified SBC approaches used by each project, and coded BCTs using validated coding protocols. Effectiveness ratios (ERs) were calculated to assess pathways, approaches, and BCTs in relation to dietary diversity outcomes (minimum diet diversity for children, child dietary diversity score, and women's dietary diversity). Results: Of 65 included NSA interventions, the most used agriculture-to-nutrition pathways included 1) agricultural production for home consumption (n = 61); 2) women's empowerment (n = 36); and 3) agricultural income (n = 37) pathways. The most used SBC approaches were interpersonal communication (IPC, n = 59) and community-based approaches (n = 53). Frequently used BCTs included "instructions on how to perform the behavior" (n = 65), "social support (unspecified)" (n = 43), and using a "credible source" (n = 43). The increased production for the home consumption pathway, IPC approach, and the BCT "behavioral practice" had high ERs for diet diversity outcomes. Conclusions: Although the agricultural production for home consumption pathway to improved nutrition had the highest ERs for diet diversity, other pathways, such as income generation and reducing wastage, hold promise and require additional investigation. The most commonly applied BCTs focused on information dissemination; however, participatory BCTs related to behavioral demonstration, and behavioral practice had higher ERs. Findings indicate a need to test less frequently utilized SBC components to determine effectiveness.This trial was registered at PROSPERO (=https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=179016) as CRD42020179016.

Our cities, our farm lands: The socioeconomic determinants of urban households participation in urban agricultural production under climatic stressors.

In Africa, urban agriculture is critical in addressing food security issues, economic and environmental sustainability in rapidly urbanizing regions such as urban Ghana. However, the factors that influence urban residents' participation in urban agricultural production under climate change adaptation has little space in the extant literature. Recognizing the increasing challenges posed by climate change, this study aims to understand the socio-economic factors influencing urban households' participation in agricultural activities and its implications for climate change adaptation and to draw urban households' socio-economic characteristics and their association with participating in urban agricultural production in the era of climate change effects in urban areas of Ghana. A quantitative approach is employed, involving a sample size of 362 urban households' across diverse neighbourhoods. Statistical analyses, including descriptive statistics-frequencies and percentages, inferential statistics-chi-square test and binary regression models, are employed to quantify the relationships between demographic factors and participation levels. The data suggests correlations between demographic variables, such as household size and income are significant at an alpha 0.05 in determining an urban household's participation in urban agricultural production under climatic stressors. Meanwhile, more urban households' in middle and high-class areas participate in urban agriculture than the lower class. Land acquisition is basically through purchasing which is a challenge in urban agriculture production. The study concluded that urban household size and monthly income are influential factors in urban households' participation in urban agricultural production even though land acquisition plays a factor. The study suggests that policymakers and stakeholders should harness the potential of urban agriculture for sustainable development in the era of climate change. This should be done through rolling out pro-poor urban development policies like pro-poor rights and legislation in urban areas; poor access to financial markets; and land tenure reforms that include flexible land holding and access by the poor.

Unlocking the potential of bioherbicides for sustainable and environment friendly weed management.

Bioherbicides might be used to manage weeds as opposed to synthetic chemical herbicides, reducing environmental risks and advancing sustainable agriculture in the meantime. Bioherbicides employ different mechanisms of action to control weeds. Microbial bioherbicides may infect and damage weed plants, disrupt their growth, or produce compounds inhibiting weed development. Plant-derived bioherbicides often target specific biochemical processes crucial for weed survival. It can be applied through conventional spraying equipment, seed treatments, or soil incorporation. Bioherbicide development faces several challenges. One major hurdle is the complex diversity of weed species across different regions, requiring tailored bioherbicide solutions. The regulatory approvals for bioherbicides can be lengthy and costly, hindering widespread adoption. Scaling up production processes and ensuring product stability also pose challenges. By reducing reliance on chemical herbicides, bioherbicides can mitigate environmental pollution, protect non-target organisms, and promote sustainable agricultural practices. The development of locally adapted bioherbicides and strategic collaborations between researchers, industries, and policymakers could further enhance their prospects in a particular country. In addition, the knowledge gaps need to be addressed prior to adopting bioherbicides in agriculture. These review intended to explore the existing state of knowledge about the categories of bioherbicides, their formulation procedure, application approaches and mode of action to control weed. The bioherbicides that are currently on the market, their effects on weed physiology, and possible factors affecting their efficacy are all included in this review. Moreover, this review offers a perspective on existing challenges and future opportunities for adopting the bioherbicides in sustainable and eco-friendly agriculture.

Vegetable crop growth under photovoltaic (PV) modules of varying transparencies.

The present study summarizes two growing seasons (2020-2021) of microclimate characterization and vegetable crop growth in an agrivoltaics system in northern Colorado, USA. The replicated experiment evaluated three module transparency types (opaque silicon [0 % transparent], bifacial silicon [∼5 % transparent], and semi-transparent cadmium telluride [40 % transparent]) plus a full sun control, and four vegetable crop species (summer squash, peppers, tomatoes, and lettuce). Air temperature under the modules in July was approximately 0.5 °C cooler than in the full sun. Soil temperature (2.5 cm depth) maximum differences were more pronounced and were 5.8 °C, 9 °C, and 14.4 °C cooler under bifacial, semi-transparent, and opaque silicon, respectively. For summer squash growing directly under the solar modules, yield was significantly reduced under each of the module transparency types. However, there was no statistically significant yield reduction for peppers, tomatoes, and lettuce indicating their suitability in an agrivoltaics system. The numerical yield of most crops increased as the transparency of the solar modules increased, which could be the focus on future work.

Hedging climate change risks in Southern Africa's agricultural industry using catastrophe bonds.

The agricultural sector is one of the key economic contributors in Southern Africa. However, agricultural production has been highly affected by climate change risks such as tropical cyclones, floods, droughts, heatwaves, hail, etc., which threaten food and nutrition security, livelihoods and business sustainability. Because of underwriting capacity problems, insurers and reinsurers have failed to provide cover for climate change risks. Also, derivatives have failed to provide a reliable option for hedging such risks. This paper explores the concept of catastrophe bonds (CAT bonds) in providing climate change risk finance. Employing the content analysis, the research shows how CAT bonds can help traditional (re)insurance in providing sufficient hedge against climate change risks and in improving disaster-preparedness, disaster risk-reduction, post-recovery initiatives and sustainable socio-economic agricultural development. The findings show that the adoption of CAT bonds can improve (re)insurers´ underwriting capacity and may enhance agricultural land policies, development, food and nutrition security and employment. Contribution: The paper shows how CAT bonds can be employed to hedge against climate change risks in agricultural production and to increase (re)insurers´ underwriting capacity. It further discusses the attractiveness of CAT bonds as another investment option for agricultural investors and how to develop and institutionalise a CAT bond market.

Risk Factor Profile for Cardiovascular Diseases Among Farmhouse Residents in Vijayapura District, Karnataka: A Cross-Sectional Study.

Background Cardiovascular illnesses are widely regarded as the leading cause of mortality worldwide, with 17.9 million deaths every year. Rheumatic heart disease, coronary heart disease, and cerebrovascular illness are among the conditions relating to the heart and blood vessels together, known as cardiovascular diseases. The main behavioural risk factors for heart disease and stroke include poor diet, inactivity, tobacco use, and excessive alcohol consumption. In contrast, one of the significant environmental risk factors is air pollution. People may experience high blood pressure and high blood sugar due to obesity and overweight as risk factors. Objectives This study aims to find the risk factor profile for cardiovascular diseases among the farmhouse residents of the Vijayapura district, India. Materials and methods A cross-sectional descriptive study was conducted with the farmhouse inhabitants of the Vijayapura district. Using a prestructured proforma that included details on the sociodemographic profile and risk factors of the farmhouse dwellers, 450 sample participants were questioned. Five families within a chosen primary sampling unit (PSU) were chosen randomly from a pool of PSUs that were picked using a probability proportional to size sampling technique. All characteristics were summarized descriptively. Results The study included 450 participants, 50.9% males and 49.1% females. The age range of 21.4% of the male participants was between 41 and 50, whereas 21.7% of the female participants belonged to the 41 to 50 age group. The study showed a proportion of them had the habit of alcohol consumption (5.8%), chewing tobacco (2%), and smoking (1.6%). Compared to female participants (1.3% vs. 1.3%), the majority of male individuals (4.8% vs. 7%) had hypertension and diabetes mellitus, respectively. Conclusion The findings of the current research indicated that the majority of farm inhabitants in the rural Vijayapura district were illiterate, belonged to a lower socioeconomic class, and had intermediate and behavioural risk factors for cardiovascular illnesses.

Novel centromeric repetitive DNA elements reveal karyotype dynamics in polyploid sainfoin (Onobrychis viciifolia).

Polyploidy is a common feature in eukaryotes with one of paramount consequences leading to better environmental adaptation. Heterochromatin is often located at telomeres and centromeres and contains repetitive DNA sequences. Sainfoin (Onobrychis viciifolia) is an important perennial forage legume for sustainable agriculture. However, there are only a few studies on the sainfoin genome and chromosomes. In this study, novel tandem repetitive DNA sequences of the sainfoin genome (OnVi180, OnVi169, OnVi176 and OnVidimer) were characterized using bioinformatics, molecular and cytogenetic approaches. The OnVi180 and OnVi169 elements colocalized within functional centromeres. The OnVi176 and OnVidimer elements were localized in centromeric, subtelomeric and interstitial regions. We constructed a sainfoin karyotype that distinguishes the seven basic chromosome groups. Our study provides the first detailed description of heterochromatin and chromosome structure of sainfoin and proposes an origin of heterozygous ancestral genomes, possibly from the same ancestral diploid species, not necessarily from different species, or for chromosome rearrangements after polyploidy. Overall, we discuss our novel and complementary findings in a polyploid crop with unique and complex chromosomal features.

Micro plastics mapping in the agricultural sector of Cyprus.

The impact of Microplastic Pollution (MPs) on human health, the environment, economy, and society has been previously investigated. However, there is a lack of comprehensive understanding regarding specific areas that require urgent measures to address marine pollution. The accumulation of MPs in Mediterranean coastal environments is particularly noteworthy. This is attributed to the region's economic reliance on tourism and the decline of popular tourist destinations caused by the presence of coastal and marine waste. The objective of the present research was to conduct a strategic analysis and mapping of MPs from soil samples taken from rural areas of Cyprus. Within the framework of the present research, a general picture of the status of MP pollution in areas covering significant percentages in the domestic supply of fruits and vegetables was obtained. The survey indicated the presence of more than 70% of MPs in crops at a concentration of up to 1.5 %. As a result of this research, the need to highlight the importance of the rational use of plastics and proper management to mitigate pollution is a primary concern. The rational separation of materials for recycling, information, reuse of materials, processing, and an increase in the number of recycling bins in public places are considered urgent. Cooperation between the state, institutions and industry must be based on the protection of people and the environment.

Power-to-vitamins: producing folate (vitamin B9) from renewable electric power and CO2 with a microbial protein system.

We recently proposed a two-stage Power-to-Protein technology to produce microbial protein from renewable electric power and CO2. Two stages were operated in series: Clostridium ljungdahlii in Stage A to reduce CO2 with H2 into acetate, and Saccharomyces cerevisiae in Stage B to utilize O2 and produce microbial protein from acetate. Renewable energy can be used to power water electrolysis to produce H2 and O2. A drawback of Stage A was the need for continuous vitamin supplementation. In this study, by using the more robust thermophilic acetogen Thermoanaerobacter kivui instead of C. ljungdahlii, vitamin supplementation was no longer needed. Additionally, S. cerevisiae produced folate when grown with acetate as a sole carbon source, achieving a total folate concentration of 6.7 mg per 100 g biomass with an average biomass concentration of 3 g l-1. The developed Power-to-Vitamin system enables folate production from renewable power and CO2 with zero or negative net-carbon emissions.

Temporal analysis of water chemistry and smallmouth bass (Micropterus dolomieu) health at two sites with divergent land use in the Susquehanna River watershed, Pennsylvania, USA.

Monitoring wild fish health and exposure effects in impacted rivers and streams with differing land use has become a valuable research tool. Smallmouth bass (Micropterus dolomieu) are a sensitive, indicator species that exhibit signs of immunosuppression and endocrine disruption in response to water quality changes and contaminant exposure. To determine the impact of agriculture and development on smallmouth bass health, two sites (a developed/agriculture site and a forested site) in the Susquehanna River watershed, Pennsylvania were selected where bass and water chemistry were sampled from 2015 to 2019. Smallmouth bass were sampled for histopathology to assess parasite and macrophage aggregate density in the liver and spleen, condition factor (Ktl), hepatic gene transcript abundance, hepatosomatic index (HSI), and a health assessment index (HAI). Land use at the developed/agriculture site included greater pesticide application rates and phytoestrogen crop cover and more detections and higher concentrations of pesticides, wastewater-associated contaminants, hormones, phytoestrogens, and mycotoxins than at the forested site. Additionally, at the developed/agriculture site, indicators of molecular changes, including oxidative stress, immune/inflammation, and lipid metabolism-related hepatic gene transcripts, were associated with more contaminants and land use variables. At both sites, there were multiple associations of contaminants with liver and/or spleen macrophage aggregate density, indicating that changes at the molecular level seemed to be a better indicator of exposures unique to each site. The findings illustrate the importance of timing for land management practices, the complex mixtures aquatic animals are exposed to, and the temporal changes in contaminant concentration. Agricultural practices that affect hepatic gene transcripts associated with immune function and disease resistance were demonstrated which could negatively affect smallmouth bass populations.

Investigating Drivers Impacting Carbon Stock and Carbon Offset in a Large-Scale Rubber Plantation in the Middle South of Thailand.

A large-scale rubber plantation in Southern Thailand is expected to capture a significant amount of carbon dioxide from emissions through carbon sinks in the vegetation and soil. The goal of this research is to create a carbon offset assessment for rubber plantations lasting for 30 years using a voluntary market contract approach. To evaluate the area of large-scale rubber plantations, this study evaluated major growing regions in five provinces in the middle-south region of Thailand (Nakhon Si Thammarat, Phatthalung, Songkhla, Satun and Trang) using an integrated RS-GIS technique that incorporated biomass allometric equations, soil series databases, and object-based classification. The classification of rubber plantation areas and the mapping of rubber stand ages were conducted to estimate the above-ground biomass of the rubber tree. Texture analysis was used in the rubber classification process, and normalised difference vegetation index (NDVI) was combined with texture analysis to separate vegetation areas from other land cover. Four groups of varying ages (1-6, 7-13, 14-20 and 21-30 years old) were evaluated for their capacity to generate carbon offsets. The equations of voluntary market contract revenue according to the contract method of the CCX were applied for this case study. This evaluation was used to estimate their annual value, total and net incomes in the carbon market price regarding the RGGI Allowance (RGA). Carbon offset income was then used to estimate the potential income (over a 30-year period) of the life of the contract. The results showed that the carbon stock potential of rubber plantations depended on the age of the trees and the soil carbon stock. The total carbon stock in the rubber plantations varied from 249.73 to 301.48 Mg C/ha (or equivalently 916.49 to 1,106.44 Mg CO2e/ha). Furthermore, the potential net income of the contract was estimated to be between USD5,378.32 and USD5,930.38 Mg CO2e/ha over a 30-year period according to the voluntary market contract revenue. These results suggest that the large agricultural land plot policy could create opportunities for carbon offsetting. The policy of large-scale rubber areas could be used as a tool and mechanism for farmers who are considering participating in carbon-crediting mechanisms. Then, farmers could use voluntary market contracts as a guide and foundation for their decision-making. The carbon offset credit strategy could assist Thailand in achieving its climate goals of transitioning to a low-carbon agriculture sector.

Effects of tomato straw fermentation on nutrients and bacterial community structure.

Unsustainable straw treatment methods detrimentally affect the environment and ecology. Aerobic fermentation (AE) and anaerobic fermentation (AN) are environmentally friendly treatments that better utilise straw resources. In this study, high-throughput sequencing was used to investigate the effects of AE and AN on nutrient content and microbial community structure during tomato straw fermentation. Nitrate nitrogen, available phosphorus, available potassium, and fulvic acid contents following AE were 1250.04 mg/kg, 80.34 %, 161.39 %, and 49.31 %, respectively, which were higher than those following AN. Ammonium nitrogen, humic acid, and humic substance levels following AN were 309.07 %, 31.18 %, and 17.38 %, respectively, which were higher than those following AE. Firmicutes (24.76 %) and Actinobacteria (12.93 %) were more abundant following AE, whereas Proteobacteria (33.82 %) and Bacteroidetes (33.82 %) exhibited higher abundance following AN. AE more effectively eliminated pathogenic bacteria (22.01%-0.26 %) and encouraged stronger interactions between dominant bacterial genera. Redundancy and Mantel test analyses revealed that electrical conductivity and temperature were the most important environmental factors affecting bacterial communities in AE and AN, respectively. AE had a stronger effect on effective nutrient release from tomato straw, implying its greater application potential as a fertiliser. Overall, our study provides a theoretical basis for the optimisation of fermentation methods and processes.