Commentary on a Classic JHC Article on the Histochemical Measurement of DNA Content in Cells.

This article comments on the significance of a highly cited review article on DNA cytochemical quantitation that was published in the Journal of Histochemistry and Cytochemistry in 2002 (David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert. From pixels to picograms: A beginners' guide to genome quantification by Feulgen image analysis densitometry.

Rapid preparation and single-cell analysis of concentrated blood smears using a high-throughput blood cell separator and a microfabricated grid film.

Cytological examination of peripheral white blood cells inhomogeneously distributed on a blood smear is currently limited by the low abundance and random sampling of the target cells. To address the challenges, we present a new approach to prepare and analyze concentrated blood smears by rapidly enriching white blood cells up to 32-fold with 92% recovery on average at a high throughput (1mL/min) using a deterministic migration-based separator and by systematically analyzing a large number of the cells distributed over a blood slide using a microfabricated grid film. We anticipate that our approach will improve the clinical utility of blood smear tests, while offering the capability to detect rare cell populations.

[The fluorescent immune cytochemical analysis of exudations and washouts from serous cavities under intra-operational cytological diagnostic.]

According publications' data, the range of diagnostic sensitivity of cytological analysis of exudates makes up to 64%-96%. In case of emergency intra-operational diagnostic, a necessity occurs to implement additional techniques. One of them is a fluorescent immune cytochemical analysis with monoclonal antibodies to BER-EP4, conjugated with fluorochrome FITC. The mentioned analysis demonstrates high diagnostic specificity and sensitivity under differential diagnostic of metastatic and reactive exudates.