RESUMEN
For the treatment of trichomonas vaginitis, a combination therapy of metronidazole vaginal effervescent tablet combined with Flavescentis Sophora suppository is proposed. The patients diagnosed with trichomonas vaginitis are divided into the conventional treatment group and the joint group. The conventional group is treated with metronidazole vaginal effervescent tablets, and the joint group is treated with metronidazole vaginal effervescent tablets combined with sophora bolt. Both groups are treated for 3 months to observe the therapeutic effect of the two treatment methods. The expressions of IL-2 and IL-13 in serum of patients are detected by the ELISA. The changes in the vaginal pH value and cleanliness at each time point are observed. The patients are followed up for 6 months after treatment in order to observe the recurrence of trichomonas vaginitis in both groups. Experimental results show that the combination of the two tablets can further improve the clinical efficacy and reduce the inflammatory response and effectively improve the PH value and cleanliness of the vagina, and the recurrence rate is lower, which is worthy of clinical application.
Asunto(s)
Sophora , Vaginitis por Trichomonas , Femenino , Humanos , Metronidazol/metabolismo , Metronidazol/uso terapéutico , Comprimidos/metabolismo , Comprimidos/uso terapéutico , Vaginitis por Trichomonas/tratamiento farmacológico , Vaginitis por Trichomonas/metabolismo , Vagina/metabolismoRESUMEN
Trichomoniasis is the third most common sexually transmitted infection in humans and is caused by the protozoan parasite, Trichomonas vaginalis (Tv). Pathogenic outcomes are more common in women and generally include mild vaginitis or cervicitis. However, more serious effects associated with trichomoniasis include adverse reproductive outcomes. Like other infectious agents, pathogenesis from Tv infection is predicted to be the result of both parasite and host factors. At the site of infection, neutrophils are the most abundant immune cells present and probably play key roles in both parasite clearance and inflammatory pathology. Here, we discuss the evidence that neutrophils home to the site of Tv infection, kill the parasite, and that in some circumstances, parasites possibly evade neutrophil-directed killing. In vitro, the parasite is killed by neutrophils using a novel antimicrobial mechanism called trogocytosis, which probably involves both innate and adaptive immunity. While mechanisms of evasion are mostly conjecture at present, the persistence of Tv infections in patients argues strongly for their existence. Additionally, many strains of Tv harbour microbial symbionts Mycoplasma hominis or Trichomonasvirus, which are both predicted to impact neutrophil responses against the parasite. Novel research tools, especially animal models, will help to reveal the true outcomes of many factors involved in neutrophil-Tv interactions during trichomoniasis.
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Interacciones Huésped-Parásitos/inmunología , Neutrófilos/inmunología , Vaginitis por Trichomonas/inmunología , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/inmunología , Animales , Apoptosis , Quimiotaxis de Leucocito/inmunología , Citotoxicidad Inmunológica , Susceptibilidad a Enfermedades/inmunología , Femenino , Humanos , Evasión Inmune , Neutrófilos/metabolismo , Neutrófilos/patología , Proteínas Opsoninas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Simbiosis , Vaginitis por Trichomonas/metabolismoRESUMEN
Trichomonas vaginalis, a human-infective parasite, causes the most prevalent nonviral sexually transmitted infection worldwide. This pathogen secretes extracellular vesicles (EVs) that mediate its interaction with host cells. Here, we have developed assays to study the interface between parasite EVs and mammalian host cells and to quantify EV internalization by mammalian cells. We show that T. vaginalis EVs interact with glycosaminoglycans on the surface of host cells and specifically bind to heparan sulfate (HS) present on host cell surface proteoglycans. Moreover, competition assays using HS or removal of HS from the host cell surface strongly inhibit EV uptake, directly demonstrating that HS proteoglycans facilitate EV internalization. We identified an abundant protein on the surface of T. vaginalis EVs, 4-α-glucanotransferase (Tv4AGT), and show using isothermal titration calorimetry that this protein binds HS. Tv4AGT also competitively inhibits EV uptake, defining it as an EV ligand critical for EV internalization. Finally, we demonstrate that T. vaginalis EV uptake is dependent on host cell cholesterol and caveolin-1 and that internalization proceeds via clathrin-independent, lipid raft-mediated endocytosis. These studies reveal mechanisms used to drive host:pathogen interactions and further our understanding of how EVs are internalized by target cells to allow cross-talk between different cell types.
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Endocitosis , Vesículas Extracelulares/metabolismo , Proteoglicanos/metabolismo , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/metabolismo , Transporte Biológico , Caveolinas/metabolismo , Colesterol/metabolismo , Femenino , Interacciones Huésped-Parásitos , Humanos , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/fisiopatología , Trichomonas vaginalis/genéticaRESUMEN
Trichomonas vaginalis is responsible for the most prevalent non-viral sexually transmitted disease worldwide, and yet the mechanisms used by this parasite to establish and maintain infection are poorly understood. We previously identified a T. vaginalis homologue (TvMIF) of a human cytokine, human macrophage migration inhibitory factor (huMIF). TvMIF mimics huMIF's role in increasing cell growth and inhibiting apoptosis in human host cells. To interrogate a role of TvMIF in parasite survival during infection, we asked whether overexpression of TvMIF (TvMIF-OE) confers an advantage to the parasite under nutrient stress conditions by comparing the survival of TvMIF-OE parasites to that of empty vector (EV) parasites. We found that under conditions of serum starvation, overexpression of TvMIF resulted in increased parasite survival. Serum-starved parasites secrete 2.5-fold more intrinsic TvMIF than unstarved parasites, stimulating autocrine and paracrine signaling. Similarly, we observed that addition of recombinant TvMIF increased the survival of the parasites in the absence of serum. Recombinant huMIF likewise increased the parasite survival in the absence of serum, indicating that the parasite may use this host survival factor to resist its own death. Moreover, TvMIF-OE parasites were found to undergo significantly less apoptosis and reactive oxygen species (ROS) generation under conditions of serum starvation, consistent with increased survival being the result of blocking ROS-induced apoptosis. These studies demonstrated that a parasitic MIF enhances survival under adverse conditions and defined TvMIF and huMIF as conserved survival factors that exhibit cross talk in host-pathogen interactions.IMPORTANCE Macrophage migration inhibitory factor (MIF) is a conserved protein found in most eukaryotes which has been well characterized in mammals but poorly studied in other eukaryotes. The limited analyses of MIF proteins found in unicellular eukaryotes have focused exclusively on the effect of parasitic MIF on the mammalian host. This was the first study to assess the function of a parasite MIF in parasite biology. We demonstrate that the Trichomonas vaginalis MIF functions to suppress cell death induced by apoptosis, thereby enhancing parasite survival under adverse conditions. Our research reveals a conserved survival mechanism, shared by a parasite and its host, and indicates a role for a conserved protein in mediating cross talk in host-pathogen interactions.
Asunto(s)
Factores Inhibidores de la Migración de Macrófagos/metabolismo , Nutrientes/metabolismo , Proteínas Protozoarias/metabolismo , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/citología , Trichomonas vaginalis/metabolismo , Apoptosis , Supervivencia Celular , Femenino , Interacciones Huésped-Patógeno , Humanos , Oxidorreductasas Intramoleculares/genética , Oxidorreductasas Intramoleculares/metabolismo , Factores Inhibidores de la Migración de Macrófagos/genética , Nutrientes/análisis , Proteínas Protozoarias/genética , Especies Reactivas de Oxígeno/metabolismo , Vaginitis por Trichomonas/genética , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/genéticaRESUMEN
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Despite the serious consequences associated with trichomoniasis disease, little is known about parasite or host factors involved in attachment of the parasite-to-host epithelial cells. Here, we report the identification of microvesicle-like structures (MVs) released by T. vaginalis. MVs are considered universal transport vehicles for intercellular communication as they can incorporate peptides, proteins, lipids, miRNA, and mRNA, all of which can be transferred to target cells through receptor-ligand interactions, fusion with the cell membrane, and delivery of a functional cargo to the cytoplasm of the target cell. In the present study, we demonstrated that T. vaginalis release MVs from the plasma and the flagellar membranes of the parasite. We performed proteomic profiling of these structures demonstrating that they possess physical characteristics similar to mammalian extracellular vesicles and might be selectively charged with specific protein content. In addition, we demonstrated that viable T. vaginalis parasites release large vesicles (LVs), membrane structures larger than 1 µm that are able to interact with other parasites and with the host cell. Finally, we show that both populations of vesicles present on the surface of T vaginalis are induced in the presence of host cells, consistent with a role in modulating cell interactions.
Asunto(s)
Vesículas Extracelulares/metabolismo , Interacciones Huésped-Parásitos , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/fisiología , Trichomonas vaginalis/ultraestructura , Comunicación Celular , Vesículas Extracelulares/química , Vesículas Extracelulares/ultraestructura , Femenino , Células HeLa , Humanos , Proteómica , Proteínas Protozoarias/análisis , Proteínas Protozoarias/metabolismo , Trichomonas vaginalis/química , Trichomonas vaginalis/citologíaRESUMEN
Polyamines are essential for many biological processes in all organisms. Here we show a current landscape of studies and strategies implemented for the study of polyamine metabolism, as well as molecular aspects that implicate the role of key enzymes, transport proteins, inhibitors, and the study of novel molecules as potential therapeutic targets. This review focused on the synthesis, interconversion and function of these molecules in Trichomonas vaginalis, a common sexually transmitted parasite of humans.
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Antiparasitarios/administración & dosificación , Sistemas de Liberación de Medicamentos/métodos , Poliaminas/metabolismo , Trichomonas vaginalis/efectos de los fármacos , Trichomonas vaginalis/metabolismo , Secuencia de Aminoácidos , Animales , Transporte Biológico/efectos de los fármacos , Transporte Biológico/fisiología , Femenino , Humanos , Poliaminas/antagonistas & inhibidores , Espermidina/metabolismo , Espermina/metabolismo , Vaginitis por Trichomonas/tratamiento farmacológico , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/genéticaRESUMEN
Trichomonas vaginalis is an extracellular eukaryotic parasite that causes the most common, non-viral sexually transmitted infection worldwide. Although disease burden is high, molecular mechanisms underlying T. vaginalis pathogenesis are poorly understood. Here, we identify a family of putative T. vaginalis rhomboid proteases and demonstrate catalytic activity for two, TvROM1 and TvROM3, using a heterologous cell cleavage assay. The two T. vaginalis intramembrane serine proteases display different subcellular localization and substrate specificities. TvROM1 is a cell surface membrane protein and cleaves atypical model rhomboid protease substrates, whereas TvROM3 appears to localize to the Golgi apparatus and recognizes a typical model substrate. To identify TvROM substrates, we interrogated the T. vaginalis surface proteome using both quantitative proteomic and bioinformatic approaches. Of the nine candidates identified, TVAG_166850 and TVAG_280090 were shown to be cleaved by TvROM1. Comparison of amino acid residues surrounding the predicted cleavage sites of TvROM1 substrates revealed a preference for small amino acids in the predicted transmembrane domain. Over-expression of TvROM1 increased attachment to and cytolysis of host ectocervical cells. Similarly, mutations that block the cleavage of a TvROM1 substrate lead to its accumulation on the cell surface and increased parasite adherence to host cells. Together, these data indicate a role for TvROM1 and its substrate(s) in modulating attachment to and lysis of host cells, which are key processes in T. vaginalis pathogenesis.
Asunto(s)
Interacciones Huésped-Parásitos/fisiología , Proteínas Protozoarias/metabolismo , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/enzimología , Femenino , Citometría de Flujo , Técnica del Anticuerpo Fluorescente Indirecta , Células HEK293 , Humanos , Mutagénesis Sitio-Dirigida , Péptido Hidrolasas/metabolismo , Trichomonas vaginalis/patogenicidadRESUMEN
Trichomonas vaginalis; induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in TNF-α production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased TNF-α production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, TNF-α production was significantly decreased compared to the control; however, TNF-α reduction patterns were different depending on the type of PI3K/MAPK inhibitors. TNF-α production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of TNF-α production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.
Asunto(s)
Cuello del Útero/parasitología , Células Epiteliales/enzimología , Sistema de Señalización de MAP Quinasas , Membrana Mucosa/enzimología , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Vaginitis por Trichomonas/enzimología , Trichomonas vaginalis/fisiología , Factor de Necrosis Tumoral alfa/metabolismo , Línea Celular , Cuello del Útero/enzimología , Cuello del Útero/metabolismo , Células Epiteliales/metabolismo , Células Epiteliales/parasitología , Femenino , Humanos , Membrana Mucosa/metabolismo , Membrana Mucosa/parasitología , Fosfatidilinositol 3-Quinasas/genética , Proteínas Proto-Oncogénicas c-akt/genética , Vaginitis por Trichomonas/genética , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/parasitología , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Trichomonas vaginalis causes vaginitis and increases the risk of HIV transmission by heterosexual sex, while Tritrichomonas foetus causes premature abortion in cattle. Our goals were to determine the effects, if any, of anti-retroviral lectins, which are designed to prevent heterosexual transmission of HIV, on adherence of Trichomonas to ectocervical cells and on Tritrichomonas infections in a mouse model. We show that Trichomonas Asn-linked glycans (N-glycans), like those of HIV, bind the mannose-binding lectin (MBL) that is part of the innate immune system. N-glycans of Trichomonas and Tritrichomonas bind anti-retroviral lectins (cyanovirin-N and griffithsin) and the 2G12 monoclonal antibody, each of which binds HIV N-glycans. Binding of cyanovirin-N appears to be independent of susceptibility to metronidazole, the major drug used to treat Trichomonas. Anti-retroviral lectins, MBL, and galectin-1 cause Trichomonas to self-aggregate and precipitate. The anti-retroviral lectins also increase adherence of ricin-resistant mutants, which are less adherent than parent cells, to ectocervical cell monolayers and to organotypic EpiVaginal tissue cells. Topical application of either anti-retroviral lectins or yeast N-glycans decreases by 40 to 70% the recovery of Tritrichomonas from the mouse vagina. These results, which are explained by a few simple models, suggest that the anti-retroviral lectins have a modest potential for preventing or treating human infections with Trichomonas.
Asunto(s)
Células Epiteliales/parasitología , Lectinas/química , Tricomoniasis/parasitología , Vaginitis por Trichomonas/parasitología , Vagina/parasitología , Animales , Antirretrovirales/química , Anticuerpos Monoclonales/química , Proteínas Bacterianas/química , Anticuerpos ampliamente neutralizantes , Proteínas Portadoras/química , Modelos Animales de Enfermedad , Células Epiteliales/citología , Femenino , Galectina 1/química , Anticuerpos Anti-VIH , Inmunidad Innata , Lectina de Unión a Manosa/química , Metronidazol/química , Ratones , Mutación , Polisacáridos/química , Ricina/química , Tricomoniasis/metabolismo , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis , Tritrichomonas foetus , Vagina/patologíaRESUMEN
INTRODUCTION: Trichomonas vaginalis infection is associated with an increased risk of HIV infection in exposed-seronegative women (ESN) despite their unique immune quiescent profile. It is important to understand possible mechanisms, such as recruitment of activated T cells, by which T. vaginalis could facilitate HIV infection in this population. METHODS: We conducted a cross-sectional study exploring the relationships between T. vaginalis infection, inflammatory markers and T cell activation in the cervix of ESN. During scheduled study visits, participants completed a behavioral questionnaire and physical exam, including sexually transmitted infection (STI) screening and collection of endocervical sponge and cytobrush specimens. T cell and monocyte phenotypes were measured in cervical cytobrush specimens using multi-parameter flow cytometry. Cervical sponge specimens were used to measure cytokines (IL-6, IL-8,IL-10, IP-10, RANTES) using Luminex immunoassays and the immune activation marker soluble TNF receptor 1 using ELISA. RESULTS: Specimens of 65 women were tested. Twenty-one of these women were infected with T. vaginalis. T. vaginalis infection was associated with significantly increased concentrations of IL-8 (1275pg/ml vs. 566pg/ml, p=.02) and sTNFr1 (430 pg/ml vs. 264 pg/ml, p=.005). However, T. vaginalis infection was not associated with increased percent expression of CCR5+ T cells nor increased CD38 and HLADR activation compared to uninfected women. It was also not associated with increased expression of CCR5+ monocytes. CONCLUSIONS: Among ESN T. vaginalis infection is associated with increased levels of genital pro-inflammatory/immune activation markers IL-8 and TNFr1, but was not associated with an increased percentage of activated endocervical T cells along the CD38 and HLADR pathways. Thus, while T.vaginalis infection may result in some reversal of the immune quiescent profile of ESN, enhanced recruitment of activated CD38 and HLADR expressing CD4+ cells into the endocervix may not be part of the mechanism by which Trichomonas infection alters HIV susceptibility in this unique subset of women.
Asunto(s)
Cuello del Útero/microbiología , Seronegatividad para VIH , Interleucina-8/metabolismo , Receptores Tipo I de Factores de Necrosis Tumoral/metabolismo , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/fisiología , Adolescente , Adulto , Estudios Transversales , Femenino , Infecciones por VIH/complicaciones , Humanos , Persona de Mediana Edad , Receptores Tipo I de Factores de Necrosis Tumoral/química , Solubilidad , Linfocitos T/citología , Vaginitis por Trichomonas/complicaciones , Vaginitis por Trichomonas/inmunología , Vaginitis por Trichomonas/virología , Replicación Viral , Adulto JovenRESUMEN
BACKGROUND: Complications related to the diagnosis and treatment of Trichomonas vaginalis infection, as well as the association between T. vaginalis infection and increased transmission of and susceptibility to human immunodeficiency virus, highlight the need for alternative interventions. We tested a human-safe, aluminum hydroxide-adjuvanted whole-cell T. vaginalis vaccine for efficacy in a BALB/c mouse model of vaginal infection. METHODS: A whole-cell T. vaginalis vaccine was administered subcutaneously to BALB/c mice, using a prime-boost vaccination schedule. CD4(+) T-cell infiltration in the murine vaginal tissue and local and systemic levels of immunoglobulins were measured at time points up to 4 weeks following infection. RESULTS: Vaccination reduced the incidence and increased the clearance of T. vaginalis infection and induced both systemic and local humoral immune responses. CD4(+) T cells were detected in vaginal tissues following intravaginal infection with T. vaginalis but were not seen in uninfected mice. The presence of CD4(+) T cells following T. vaginalis infection can potentially increase susceptibility to and transmission of human immunodeficiency virus. CONCLUSIONS: The vaccine induces local and systemic immune responses and confers significantly greater protection against vaginal infection than seen in unvaccinated mice (P < .005). These data support the potential for a human vaccine against T. vaginalis infection that could also influence the incidence of human immunodeficiency virus infection.
Asunto(s)
Linfocitos T CD4-Positivos/inmunología , Infecciones por VIH/transmisión , Vaginitis por Trichomonas/prevención & control , Trichomonas vaginalis/inmunología , Animales , Anticuerpos Antiprotozoarios/metabolismo , Movimiento Celular , Femenino , Infecciones por VIH/prevención & control , Humanos , Ratones Endogámicos BALB C , Vacunas Antiprotozoos/administración & dosificación , Vaginitis por Trichomonas/inmunología , Vaginitis por Trichomonas/metabolismo , Vacunación , Vagina/inmunología , Vagina/metabolismo , Vagina/parasitologíaRESUMEN
Trichomonas vaginalis is a sexually transmitted flagellated protist parasite responsible for trichomoniasis. This parasite is dependent on high levels of iron, favoring its growth and multiplication. Iron also differentially regulates some trichomonad virulence properties by unknown mechanisms. However, there is evidence to support the existence of gene regulatory mechanisms at the transcriptional and posttranscriptional levels that are mediated by iron concentration in T. vaginalis. Thus, the goal of this study was to identify an RNA-binding protein in T. vaginalis that interacts with the tvcp4 RNA stem-loop structure, which may participate in a posttranscriptional iron regulatory mechanism mediated by RNA-protein interactions. We performed RNA electrophoretic mobility shift assay (REMSA) and supershift, UV cross-linking, Northwestern blot, and western blot (WB) assays using cytoplasmic protein extracts from T. vaginalis with the tvcp4 RNA hairpin structure as a probe. We identified a 135-kDa protein isolated by the UV cross-linking assays as α-actinin 3 (TvACTN3) by MALDI-TOF-MS that was confirmed by LS-MS/MS and de novo sequencing. TvACTN3 is a cytoplasmic protein that specifically binds to hairpin RNA structures from trichomonads and humans when the parasites are grown under iron-depleted conditions. Thus, TvACTN3 could participate in the regulation of gene expression by iron in T. vaginalis through a parallel posttranscriptional mechanism similar to that of the IRE/IRP system.
Asunto(s)
Actinina/metabolismo , Hierro/metabolismo , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/metabolismo , Femenino , Regulación de la Expresión Génica , Humanos , Hierro/química , ARN/química , Procesamiento Postranscripcional del ARN/genética , Proteínas de Unión al ARN/metabolismo , Vaginitis por Trichomonas/parasitología , Vaginitis por Trichomonas/patologíaRESUMEN
This review focused on potential regulatory mechanisms of Trichomonas vaginalis virulence properties, cytoadherence, cytotoxicity, phagocytosis, hemolysis, induction of apoptosis, and immune evasion in response to environmental factors of the human urogenital tract, iron, zinc, and polyamines. Understanding the multifactorial nature of trichomonal pathogenesis and its regulation may help to unravel the survival strategies of trichomonads and to implement prevention policies, opportune diagnosis, and alternative treatments for control of trichomoniasis.
Asunto(s)
Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/patogenicidad , Factores de Virulencia/fisiología , Femenino , Humanos , Vaginitis por Trichomonas/metabolismo , Trichomonas vaginalis/metabolismo , Factores de Virulencia/metabolismoRESUMEN
Trichomonas vaginalis has high iron-dependency, favoring its growth and multiplication in culture. Iron also regulates some of the trichomonal virulence properties by yet unknown mechanisms. Iron is an essential but potentially toxic metal for the majority of organisms. Thus, its concentration must be tightly regulated within the cell. In mammals, the iron homeostasis is mainly regulated at the post-transcriptional level by a well known mechanism mediated by the binding of iron regulatory proteins (IRP1 and IRP2) to hairpin-loop structures, dubbed iron-responsive elements (IREs), localized in the untranslated regions (UTRs) of target mRNAs. The knowledge of iron regulation in T. vaginalis is still very limited. An iron-responsive promoter and other regulatory elements in the 5'-UTR of the ap65-1 gene were identified as a mechanism for the positive transcriptional regulation of trichomonad genes by iron. Recently, two IRE-like hairpin-loop structures in mRNAs of differentially iron-regulated TVCP4 and TVCP12 cysteine proteinases, as well as IRP-like trichomonad proteins were identified in T. vaginalis, suggesting the existence in this protozoan of a post-transcriptional iron regulatory mechanism by an IRE/IRP-like system. The responsiveness of T. vaginalis to distinct iron concentrations was examined here. Also, the comparison of the atypical IRE-like sequences of T. vaginalis with the consensus IRE and other putative IRE sequences present in parasite and bacteria mRNAs suggest that these trichomonad IRE-like sequences might be the ancestral forms of the RNA stem-loop structures of the IRE/IRP system.
Asunto(s)
Homeostasis , Proteínas Reguladoras del Hierro/metabolismo , Hierro/metabolismo , Transcripción Genética , Trichomonas vaginalis/fisiología , Animales , Secuencia de Bases , Moléculas de Adhesión Celular/genética , Moléculas de Adhesión Celular/metabolismo , Proteasas de Cisteína/genética , Proteasas de Cisteína/metabolismo , ADN Protozoario/análisis , ADN Protozoario/genética , Femenino , Regulación de la Expresión Génica , Genoma de Protozoos , Humanos , Hierro/química , Proteínas Reguladoras del Hierro/genética , Datos de Secuencia Molecular , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/química , Trichomonas vaginalis/patogenicidad , VirulenciaRESUMEN
OBJECTIVE: The aim of the present study was to evaluate the expression of pan-cadherin and beta-catenin in cervical smears with various types of infectious agents. PATIENTS AND METHODS: Cervical smears obtained from 53 women, aged 21-65 years, with a diagnosis of specific inflammation were examined in our study. Eighteen subjects were infected by Candida albicans, 18 by Gardnerella vaginalis, nine by Bacteroides spp. and eight by Chlamydia trachomatis. All infectious agents found in the smears were at the same time confirmed by the microbiological laboratory methods. We performed a biotin-streptavidin-peroxidase immunocytochemical method using anti-beta-catenin (Clone 12F7) and anti-pan-cadherin (pan, polyclonal) antibodies. RESULTS: Aberrant expression of pan-cadherin was found in the cytoplasmic membrane of glandular, metaplastic, superficial and intermediate squamous cells in all types of infections. With regard to beta-catenin, this was expressed in majority (90%) of glandular and metaplastic cells in all types of infections and in a small proportion (15%) of superficial and intermediate squamous cells in infections caused by C. albicans and G. vaginalis. CONCLUSION: Our data show that infectious agents may cause alterations in the expression and distribution of these adhesive molecules, which can be recognized in cervical smears. Additional studies in larger sets of patients should help clarify this issue further.
Asunto(s)
Cadherinas/biosíntesis , Cervicitis Uterina/metabolismo , beta Catenina/biosíntesis , Adulto , Anciano , Infecciones por Bacteroides/metabolismo , Infecciones por Bacteroides/fisiopatología , Candidiasis/metabolismo , Candidiasis/fisiopatología , Infecciones por Chlamydia/metabolismo , Infecciones por Chlamydia/fisiopatología , Femenino , Humanos , Inmunohistoquímica , Inflamación/metabolismo , Persona de Mediana Edad , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/fisiopatología , Cervicitis Uterina/fisiopatología , Frotis VaginalRESUMEN
BACKGROUND: Bacterial vaginosis and Trichomons vaginalis are believed to be the risk factors for preterm labor birth and preterm prelabor rupture of membranes. The objective of this study was to investigate the association between bacterial vaginosis, T.vaginalis, and vaginal pH with preterm labor birth and preterm prelabor rupture of membranes after excluding other known risk factors. METHODS: In this cohort study, we enrolled 1223 pregnant women with gestational age of 16 - 36 weeks from Amir-Almomenin General Hospital in Semnan, Iran, who had no known medical risk factors for preterm labor birth. Bacterial vaginosis and T.vaginalis were determined on the basis of vaginal pH, saline wet mount, and Amsel tests. The principal outcome was delivery before 37 weeks of gestation and preterm prelabor rupture of membranes. RESULTS: Bacterial vaginosis and T.vaginalis were detected in 16.0% and 5.5% of these women, respectively. Bacterial vaginosis was positive in 65 (33.1%) patients at 16 - 20 weeks of gestation and in 134 (66.9%) patients at 36 weeks of gestation or during labor (developing preterm labor birth or preterm prelabor rupture of membranes). The frequencies for T.vaginalis were 20 (29.9%) and 47 (70.1%) patients, respectively. All patients with bacterial vaginosis and T.vaginalis had a vaginal pH > or = 5. There was a significant correlation between bacterial vaginosis and vaginal pH > or = 5, with preterm labor birth (OR: 5.99; CI: 3.79 - 9.49) and preterm prelabor rupture of membranes (OR: 2.34; CI: 1.07 - 4.99). Moreover, a significant correlation was found between vaginal pH > or = 5 with preterm labor birth (OR: 5.82; CI: 2.96 - 11.39) and preterm prelabor rupture of membranes (OR: 4.11; CI: 1.62 - 10.12). There was no significant correlation between T.vaginalis with preterm labor birth (OR: 0.73; CI: 0.22 - 2.17) and preterm prelabor rupture of membranes (OR: 1.22; CI: 0.29 - 5.05). CONCLUSION: The presence of bacterial vaginosis or vaginal pH > or = 5 at 16 - 36 weeks of gestation is associated with an increased risk of preterm labor birth and preterm prelabor rupture of membranes.
Asunto(s)
Complicaciones Infecciosas del Embarazo , Resultado del Embarazo , Vaginitis por Trichomonas/microbiología , Trichomonas vaginalis/aislamiento & purificación , Vagina/metabolismo , Adulto , Animales , Femenino , Estudios de Seguimiento , Edad Gestacional , Humanos , Concentración de Iones de Hidrógeno , Embarazo , Estudios Retrospectivos , Factores de Riesgo , Vaginitis por Trichomonas/metabolismo , Vagina/microbiologíaRESUMEN
Trichomonas vaginalis parasitizes in human genitourinary tract. The protozoon adhering to target cell plays a critical role in its contact-dependent cytotoxicity. The enzymes synthesized by T.voginalis can hurt vaginalis epithelial cells (VECs) directly. The focal immune reaction in the location parasitized by the parasite may provide an immunologic protection. Meanwhile, inflammatory factors and immune cells may aggravate the situation. In general, the T. vaginalis-induced contact-dependent cytotoxicity is a result of the involvement of some molecular and chemical factors.
Asunto(s)
Apoptosis , Vaginitis por Trichomonas/patología , Trichomonas vaginalis/crecimiento & desarrollo , Animales , Proteínas Reguladoras de la Apoptosis/metabolismo , Moléculas de Adhesión Celular/metabolismo , Supervivencia Celular , Cisteína Endopeptidasas/metabolismo , Femenino , Humanos , Proteínas Protozoarias/metabolismo , Vaginitis por Trichomonas/metabolismo , Vaginitis por Trichomonas/parasitología , Trichomonas vaginalis/metabolismoRESUMEN
Recurrent acute pancreatitis associated with metronidazole developed in a 49-year-old woman who was taking the drug as treatment for vaginal trichomoniasis. The lack of alternative effective therapies for trichomoniasis governed the decision to rechallenge the patient with metronidazole despite a vague history of this reaction on a previous occasion. Six reports of this reaction are found in the literature. The patient was admitted to the hospital 12 hours after taking a single dose of metronidazole. Severe epigastric pain and elevated amylase and lipase concentrations led to the diagnosis of acute pancreatitis, although results of an abdominal ultrasound were unremarkable. The patient made a full recovery. Although this reaction occurs infrequently, this case report illustrates the need to develop additional therapies for treatment of trichomoniasis.
Asunto(s)
Metronidazol/efectos adversos , Pancreatitis/inducido químicamente , Vaginitis por Trichomonas/tratamiento farmacológico , Femenino , Humanos , Metronidazol/uso terapéutico , Persona de Mediana Edad , Pancreatitis/metabolismo , Vaginitis por Trichomonas/metabolismoRESUMEN
We measured the PO2, pH, and temperature in the vaginal canals of nine patients with symptomatic Trichomonas vaginitis and those of 10 healthy women. The patients included eight women with primary infections caused by metronidazole-susceptible strains and one refractory case that resulted from infection with a metronidazole-resistant Trichomonas vaginalis. The median vaginal PO2, pH, and temperature in the patient group were 1 mm Hg, 6, and 37.3 degrees C respectively; these medians were 1 mm Hg, less than or equal to 4.5, and 37.2 degrees C in the healthy group. These data show that vaginal environment is anaerobic or microaerophilic (it has reduced oxygen tension). Because the activity of metronidazole is reduced under aerobiosis, the vaginal environment should enhance the biologic activity of the drug.