RÉSUMÉ
The efficiency of combinations of cytostatics cisplatin and adriamycin with antioxidant sodium 3-(3'-tert-butyl-4-hydroxyphenyl)propyl thiosulfate (TS-13), and nitric oxide (NO) donor NaNO2 was evaluated on two drug-resistant strains of leukemia P388 with changed redox-status of cells. Simultaneous use of both NO donor and TS-13 in combinations with the cytostatics did not increase the efficiency of therapy. In addition, antioxidant activity of TS-13, NaNO2, and their combinations was studied by the method of luminol-dependent chemiluminescence on the model systems with the use of the homogenized cells of sensitive strain and two drug-resistant strains of leukemia P388. It was shown that TS-13 and NO donor produced opposite effects: TS-13 decreased, while NO donor increased the content of free radicals in the model system. Combinations of antioxidant TS-13 and NO donor should be used with consideration for the redox-status of tumor treated.
Sujet(s)
Antioxydants , Cisplatine , Doxorubicine , Résistance aux médicaments antinéoplasiques , Leucémie P388 , Donneur d'oxyde nitrique , Oxydoréduction , Animaux , Souris , Oxydoréduction/effets des médicaments et des substances chimiques , Résistance aux médicaments antinéoplasiques/effets des médicaments et des substances chimiques , Antioxydants/pharmacologie , Doxorubicine/pharmacologie , Leucémie P388/traitement médicamenteux , Leucémie P388/anatomopathologie , Cisplatine/pharmacologie , Cisplatine/usage thérapeutique , Donneur d'oxyde nitrique/pharmacologie , Thiosulfates/pharmacologie , Nitrite de sodium/pharmacologie , Lignée cellulaire tumorale , Antinéoplasiques/pharmacologie , Protocoles de polychimiothérapie antinéoplasique/pharmacologieRÉSUMÉ
OBJECTIVE: To conduct a comparative analysis of psychopharmacotherapy effectiveness in hypochondriac disorders of late age and to identify the optimal combinations of drugs depending on the thymopathic (hypothymic and/or anxiety) components accompanying the main hypochondriac manifestation. MATERIAL AND METHODS: One hundred and eight female inpatients, aged from 50 to 88 years, with leading hypochondriac symptoms of non-delusional level were enrolled in the study. All patients were examined clinically and psychopathologically using psychometric scales: the Montgomery-Asberg Depression Rating Scale, the Hamilton Anxiety Rating Scale, the hypochondria rating scale and the UKU side-effect rating scale. RESULTS: Based on the psychometric assessment, the patients were divided into the following groups: group 1 - subjects with hypochondriac symptoms without accented thymopathic component (n=18); group 2 - subjects with a high level of hypothymia (n=49); group 3 - subjects with a high level of anxiety (n=22); group 4 - subjects with a high level of both anxiety and depression (n=19). Hypochondriac disorder without a thymopathic component was treated with monotherapy with antipsychotic drugs in low therapeutic dosages or with a combination of antipsychotic drugs in low therapeutic dosages with tricyclic antidepressants in low and medium dosages. Hypothymic hypochondriac disorders were treated with antidepressants of mainly SSRIs group and of tricyclic structure in combination with typical and atypical antipsychotic drugs in low therapeutic dosages. In these patients monotherapy with antidepressants or a combination of different antidepressants was effective in rare cases only. Hypochondriac disorder with an anxiety component was significantly more often treated by complex therapy with the addition of anxiolytic drugs, as well as a combination of antidepressants with antiptychotic drugs. Mixed hypochondriac states were treated with a combination of antidepressants and antipsychotic drugs or complex therapy with the addition of an anxiolytic drug. CONCLUSION: Hypochondriac disorders of late age in most cases are accompanied by depressive and/or anxiety symptoms, which must be taken into account for improvement of diagnostic effectiveness and relevant selection of therapy.
Sujet(s)
Neuroleptiques , Préparations pharmaceutiques , Sujet âgé , Sujet âgé de 80 ans ou plus , Antidépresseurs/usage thérapeutique , Antidépresseurs tricycliques/usage thérapeutique , Neuroleptiques/usage thérapeutique , Femelle , Humains , Adulte d'âge moyen , Inbiteurs sélectifs de la recapture de la sérotonine/usage thérapeutiqueRÉSUMÉ
Combined treatment of murine leukemia P388 with doxorubicin and platinum(IV)-nitroxyl complex ÐС118 administered in low doses improved efficiency of treatment (cure of 83% of animals) without increasing toxicity.
Sujet(s)
Protocoles de polychimiothérapie antinéoplasique/pharmacologie , Cytostatiques/pharmacologie , Doxorubicine/pharmacologie , Leucémie P388/traitement médicamenteux , Composés organiques du platine/pharmacologie , Animaux , Calendrier d'administration des médicaments , Leucémie P388/mortalité , Leucémie P388/anatomopathologie , Longévité/effets des médicaments et des substances chimiques , Souris , Souris de lignée DBA , Analyse de survieRÉSUMÉ
We measured the content of ROS and malondialdehyde in cells of in vivo drug-resistant murine P388 leukemia strains. It was found that the strains did not differ by malondialdehyde concentration, but intracellular concentration of ROS in cells of the cyclophosphamide-resistant strain (P388/CP) was higher than in cells of the original (P388) and other studied strains (P388/Rub, P388/cPt). Nuclear localization of the transcription factor Nrf2 in cells of strain P388/CP attested to its constitutive activation. Enhanced relative expression of the GCLM gene was found in all studied drug-resistant strains; the expression of the GSR and GPX1 genes was increased only in cells of the cyclophosphamide-resistant strain. These findings suggest that the mechanism of resistance of strain P388/CP is associated with increased activity of glutathione metabolism that developed as a result of activation of the antioxidant response transcription factor Nrf2 against the background of high intracellular concentration of ROS.
Sujet(s)
Glutathion/métabolisme , Facteur-2 apparenté à NF-E2/métabolisme , Animaux , Antioxydants/métabolisme , Lignée cellulaire tumorale , Cyclophosphamide/métabolisme , Malonaldéhyde/métabolisme , Souris , Oxydoréduction , Espèces réactives de l'oxygène/métabolismeRÉSUMÉ
Activities of superoxide dismutase and catalase and content of reduced glutathione in cells of drug-resistant murine leukemia P388 strains were studied without or after administration of antitumor compounds. In the absence of chemotherapeutic agents, no significant differences in activities of the studied enzymes in cells of the initial strain and strains resistant to cyclophosphamide, cisplatin, and rubomycin were observed. Compounds to which resistance was developed did not significantly affect activity of enzymes in cells of drug-resistant strains, while the use of compounds that were not resistance inductors was accompanied by a significant decrease in enzyme activity in cells resistant to cisplatin and rubomycin. In cells of strains resistant to cisplatin and cyclophosphamide, the content of reduced glutathione significantly differed from that in the initial strain. In addition, the concentration of reduced glutathione in cells of cyclophosphamide-resistant strain considerably decreased upon addition of the drug producing a therapeutic effect. Our findings suggest that the mechanism of resistance of in vivo derived cyclophosphamide resistant cell strain is related to increased level of reduced glutathione and activity of its metabolism.
Sujet(s)
Antinéoplasiques/pharmacologie , Catalase/métabolisme , Résistance aux médicaments antinéoplasiques/physiologie , Glutathion/analyse , Leucémie P388/traitement médicamenteux , Superoxide dismutase/métabolisme , Animaux , Antioxydants/métabolisme , Lignée cellulaire tumorale , Cisplatine/pharmacologie , Cyclophosphamide/pharmacologie , Daunorubicine/pharmacologie , Doxorubicine/pharmacologie , Souris , Souris de lignée DBA , Espèces réactives de l'oxygène/métabolismeRÉSUMÉ
We studied the effectiveness of cyclic hydroxamic acid CHA-5 against drug-resistant and multidrug-resistant murine P388 leukemia strains. More than 60% mice receiving transplantation of rubomycin-resistant leukemia P388 strain survived after CHA-5 monotherapy; combined therapy with CHA-5 and cisplatin was also highly effective. Vincristine-resistant tumor was highly sensitive to combined treatment with CHA-5 and cyclophosphamide. It should be emphasized that standard antitumor agents were used in very low doses in combination therapy and CHA-5 significantly potentiated their effect.
Sujet(s)
Antinéoplasiques/pharmacologie , Cisplatine/pharmacologie , Cyclophosphamide/pharmacologie , Résistance aux médicaments antinéoplasiques/effets des médicaments et des substances chimiques , Acides hydroxamiques/pharmacologie , Leucémie P388/traitement médicamenteux , Animaux , Antinéoplasiques/synthèse chimique , Daunorubicine/pharmacologie , Calendrier d'administration des médicaments , Association médicamenteuse , Synergie des médicaments , Acides hydroxamiques/synthèse chimique , Leucémie P388/mortalité , Leucémie P388/anatomopathologie , Souris , Analyse de survie , Vincristine/pharmacologieRÉSUMÉ
The clinical material obtained surgically in patients with kidney stone disease (KSD) was tested for content of the stone microflora using PCR and standard microbiological methods. It was demonstrated that about 50% of stones in patients with KSD were infected with various infection agents as observed using standard microbiological and molecular genetic methods. The percentage of detection of the Mycoplasma hominis using cultural method is lower than the percentage detected using PCR, which is due to difficult isolation and cultivation, as well as DNA fragments of mycoplasma observed after antibiotic therapy. Studies based on modern microscopy methods showed that microorganisms on the surface of the kidney stone formed multispecies biofilms.
Sujet(s)
Calculs rénaux/microbiologie , Antibactériens/usage thérapeutique , Techniques bactériologiques , Biofilms/effets des médicaments et des substances chimiques , Humains , Calculs rénaux/chirurgie , Consortiums microbiens/physiologie , Microscopie électronique , Mycoplasma hominis/génétique , Mycoplasma hominis/isolement et purification , Mycoplasma hominis/physiologie , Réaction de polymérisation en chaîne , Ureaplasma/génétique , Ureaplasma/isolement et purification , Ureaplasma/physiologieRÉSUMÉ
AIM: Use of a complex of methods for etiologic deciphering of an acute respiratory infection. MATERIALS AND METHODS: Clinical samples of blood sera, nasopharynx washes and sputum were obtained from 35 patients with acute respiratory disease (ARD). "Difco PPLO Broth" was used for M. pneumoniae cultivation. AHR, IFR, PCR, IFA were used in the study. RESULTS: Results of the study have shown that M. pneumoniae antigens in blood, sera samples were detected in AHR in 32 patients, and specific G and M class antibodies--in 21 and 18 cases, respectively. Simultaneous detection of IgG and IgM was registered in 14 patients. M. pneumoniae cell DNA was detected in 10 of 20 blood sera samples. Circulating immune complexes were isolated from blood sera of 8 patients (4 with pneumonia, 4 with ARD) and M. pneumoniae antigens were detected in them by using direct-IFR. IFR study of sputum and nasopharynx smears has shown that M. pneumoniae antigens were detected in 29 of 35 samples. In 12 of 15 smear samples M. pneumoniae. DNA was detected by PCR. In 10 cases results of antigen detection by IFR as well as DNA in PCR coincided. Results of analysis of all the clinical material have shown that in 33 of 35 patients positive results coincided for 2 or 3 and in some cases 4 of the laboratory study methods used. CONCLUSION: The use of diagnostic test complex significantly increases the accuracy of the study results, and detection of specific antibodies allows to determine disease period.
Sujet(s)
Anticorps antibactériens/sang , Antigènes bactériens/sang , Mycoplasma pneumoniae/immunologie , Pneumopathie à mycoplasmes/diagnostic , Adulte , Complexe antigène-anticorps/sang , Infections communautaires , Technique d'immunofluorescence directe , Tests d'hémagglutination , Humains , Immunoglobuline G/sang , Immunoglobuline M/sang , Mâle , Adulte d'âge moyen , Mycoplasma pneumoniae/génétique , Partie nasale du pharynx/microbiologie , Pneumopathie à mycoplasmes/immunologie , Pneumopathie à mycoplasmes/microbiologie , Réaction de polymérisation en chaîne , Expectoration/microbiologieRÉSUMÉ
In experimental animals with tumors it was studied antitumor activity of spirocyclic hydroxamic acids which could be classified as targeted agents as their target was enzyme histonedeacetylase, which was involved in the neoplastic process. The results showed that the hydroxamic acids were chemosensitizers for anticancer agents increasing their efficacy and enabling the researchers to reduce significantly the therapeutic dose. Also it was showed that hydroxamic acid, containing nitrogen mustard, was effective in the action on tumors with phenotype and genotype of multidrug resistance.
Sujet(s)
Antinéoplasiques/pharmacologie , Multirésistance aux médicaments/effets des médicaments et des substances chimiques , Résistance aux médicaments antinéoplasiques/effets des médicaments et des substances chimiques , Acides hydroxamiques/pharmacologie , Tumeurs expérimentales/traitement médicamenteux , Animaux , Antinéoplasiques/usage thérapeutique , Cyclophosphamide/administration et posologie , Inhibiteurs de désacétylase d'histone , Acides hydroxamiques/usage thérapeutique , Leucémie P388/traitement médicamenteux , Méthotrexate/administration et posologieRÉSUMÉ
AIM: Study of possibility of generalization of mycoplasma infection in patients with urogenital pathology. MATERIALS AND METHODS: Among the examined patients 5 males characterized by risky sexual behavior with pronounced symptoms of infection or without those were selected. Patients were examined by a complex of methods for the presence of mycoplasma infection by culture, PCR, DFA, PHA, AHR and by detection of specific immune complexes in blood sera. Scrapes from urogenital tract, blood sera samples, urine, saliva, prostatic fluid were materials for the study. RESULTS: In blood of all patients in ELISA antibodies against Mycoplasma hominis were detected; in PHA they were detected only in 2 individuals. In all the patients in blood CIC were detected including antigens and DNA of one or several mycoplasma species. Sperm of 3 individuals was infected by Ureaplasma spp., 2--M. genitalium. In saliva of 2 individuals M. hominis was detected, 3--U. urealyticum. CONCLUSION: In all the examined patients the infection was shown to have generalized character. This phenomenon presents itself as quite significant because mycoplasma may cause anti-apoptotic and oncogenic effect.
Sujet(s)
Infections à Mycoplasma/microbiologie , Mycoplasma genitalium/isolement et purification , Mycoplasma hominis/isolement et purification , Infections à Ureaplasma/microbiologie , Ureaplasma urealyticum/isolement et purification , Adulte , Anticorps antibactériens/sang , Complexe antigène-anticorps/sang , Test ELISA , Humains , Mâle , Infections à Mycoplasma/sang , Infections à Mycoplasma/immunologie , Infections à Mycoplasma/urine , Mycoplasma genitalium/croissance et développement , Mycoplasma hominis/croissance et développement , Réaction de polymérisation en chaîne , Prostate/métabolisme , Prostate/microbiologie , Prise de risque , Salive/microbiologie , Spermatozoïdes/microbiologie , Infections à Ureaplasma/sang , Infections à Ureaplasma/immunologie , Infections à Ureaplasma/urine , Ureaplasma urealyticum/croissance et développementRÉSUMÉ
AIM: Study the possibility of prolonged conservation in macroorganism of antigens, mycoplasma cell DNA and live pathogen cells as part of CIC against the background of persisting antigen biostructures. MATERIALS AND METHODS: Aggregate-hemagglutination, direct immunofluorescence reactions and PCR method were used to determine antigens and DNA. Circulating immune complexes from blood sera samples were isolated by M. Digeon et al., mycoplasma isolation from CIC was carried out in SP-4 medium, species identity of the isolated mini-colonies was confirmed by real-time PCR method. RESULTS: In patients with urogenital and respiratory pathology the frequency of detection of Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma pneumoniae in free state was 63.3, 53.1 and 80.82% of cases, respectively. Specific CIC in patients with verified respiratory mycoplasmosis 1 month after the onset of the disease were registered in patients with severe course of the disease, bronchitis and diseases of upper respiratory tract--in 92.5, 74.7 and 25.7% of cases, respectively. In children, bronchial asthma patients the frequency of detection of antigens and DNA of M. pneumoniae cells in free state was 72.6 and 12.33%, as part of CIC--in 60.27 and 43.8% of cases, respectively. Antigens and DNA of M. hominis in blood of this group of patients were detected in 32.9 and 26.02%, as part of CIC--in 53.42 and 52.05% of cases, respectively. During repeated examination of 12 children after etiotropic therapy execution (generally in 1.5 - 6 months) in 75% of cases antigens of both M. pneumoniae and M. hominis were detected in free state and as part of CIC. DNA of cells of these mycoplasma species were detected in 20 and 33%, as part of CIC--in41.6 and 50% of cases, respectively. In 5 patients after 6 months (after 1 year in 1 case) mycoplasma antigens and DNA were identified in CIC or in blood sera. During cultivation of CIC components precipitated from 5 blood samples of patients of this group containing M. hominis DNA, culture of M. hominis mini-colonies were isolated in 4 cases. CONCLUSION: The possibility of prolonged persistence of antigens, DNA and whole mycoplasma cells in both free state and as part of CIC in patients with respiratory and urogenital pathology was shown. CIC are thus a peculiar depot, a place of conservation of not only various mycoplasma cell components, but also live cells.
Sujet(s)
Complexe antigène-anticorps/sang , Antigènes bactériens/sang , Asthme/sang , ADN bactérien/sang , Infections à Mycoplasma/sang , Infections de l'appareil respiratoire/sang , Infections à Ureaplasma/sang , Adolescent , Adulte , Antibactériens/pharmacologie , Antibactériens/usage thérapeutique , Asthme/traitement médicamenteux , Asthme/immunologie , Asthme/microbiologie , Enfant , Enfant d'âge préscolaire , Femelle , Tests d'hémagglutination , Humains , Nourrisson , Mâle , Infections à Mycoplasma/traitement médicamenteux , Infections à Mycoplasma/immunologie , Infections à Mycoplasma/microbiologie , Mycoplasma hominis/croissance et développement , Mycoplasma hominis/isolement et purification , Mycoplasma pneumoniae/croissance et développement , Mycoplasma pneumoniae/isolement et purification , Réaction de polymérisation en chaîne , Infections de l'appareil respiratoire/traitement médicamenteux , Infections de l'appareil respiratoire/immunologie , Infections de l'appareil respiratoire/microbiologie , Infections à Ureaplasma/traitement médicamenteux , Infections à Ureaplasma/immunologie , Infections à Ureaplasma/microbiologie , Ureaplasma urealyticum/croissance et développement , Ureaplasma urealyticum/isolement et purificationRÉSUMÉ
AIM: Study previously unknown forms of persistence of Mycoplasma hominis in host organism. MATERIALS AND METHODS: Culture method was used for detection of mycoplasmas. Identification was carried out by serological, electron microscopy methods, classic PCR and real time PCR; circulating immune complexes (CIC) were isolated by PEG precipitation. RESULTS: Classic micoplasma cultures could not be isolated from blood even once. At the same time "mini-colony" cultures composed of mini-cells that were hardly passaged but sometimes formed continuous layer of the same colonies were isolated from blood serum samples with high frequency. During reseeding for more than 1 year they never acquired classic form. Not only antigens of M. hominis but its DNA were shown to be present in CIC. Viable cells forming "mini-colonies" identical to those isolated from blood sera were isolated from circulating immune complexes. A system of evidence on identity of isolated M. hominis cultures is presented. Cultures had infectivity and an ability to persist in organs of experimentally infected mice. CONCLUSION: The isolated forms are apparently the result of adaptation of mycoplasmas to humoral immunity factors.
Sujet(s)
ADN bactérien/analyse , Infections à Mycoplasma/sang , Mycoplasma hominis/génétique , ARN ribosomique 16S/analyse , Adaptation physiologique/immunologie , Animaux , Complexe antigène-anticorps/sang , Précipitation chimique , Humains , Immunité humorale , Souris , Microscopie électronique , Infections à Mycoplasma/immunologie , Infections à Mycoplasma/microbiologie , Mycoplasma hominis/isolement et purification , Mycoplasma hominis/pathogénicité , Polyéthylène glycols/composition chimique , Réaction de polymérisation en chaine en temps réelRÉSUMÉ
Treatment with low doses (1/10 of LD50) of cisplatin and platinum (IV)-nitroxyl complex VS118 [e-ammin-d-(4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl)-a,f-bi s(acetate)-b,c-dichlorplatinum (IV)] was followed by a synergistic therapeutic effect (a 100% cure of animals) as compared with monotherapy with either drug. There was no synergistic increase in toxicity. The rates of resistance development decreased in the following order: P388/cPt+VS118, P388/cPt, P388/VS118. Resistant strains P388/cPt+VS18 and P388/VS118 were highly sensitive to doxorubicin, etoposide and cyclophoshamide. Further research in cPt+VS 118 combinations should be continued.
Sujet(s)
Antinéoplasiques/pharmacologie , Cisplatine/pharmacologie , Résistance aux médicaments antinéoplasiques , Leucémie P388/traitement médicamenteux , Composés organiques du platine/pharmacologie , Composés du platine/pharmacologie , Animaux , Antinéoplasiques/administration et posologie , Antinéoplasiques/effets indésirables , Protocoles de polychimiothérapie antinéoplasique/pharmacologie , Cisplatine/administration et posologie , Cisplatine/effets indésirables , Cyclophosphamide/pharmacologie , Doxorubicine/pharmacologie , Synergie des médicaments , Étoposide/pharmacologie , Souris , Oxydes d'azote/administration et posologie , Oxydes d'azote/effets indésirables , Oxydes d'azote/pharmacologie , Composés organiques du platine/administration et posologie , Composés du platine/administration et posologie , Composés du platine/effets indésirablesRÉSUMÉ
The antigens, DNA and RNA of mycoplasmas are preset in the blood serum of persons infected with urogenital mycoplasmas. The planting of patients' tests of blood serum containing antigen M. hominis on the artificial growth mediums resulted in the growth of mini-colonies of mini-cells (20-50 nm). The colonies subcultured hardly but sometimes formed solid bacterial lawn though never acquired "fried-egg" classical mycoplasma form. The proofs of identity of these colonies to M. hominis are presented. The mini-cells possessed infectiousness and ability to persist on a long-run in the internal organs of experimentally infected mice. Apparently, mini-cells are formed under impact of stress factors of the host immune defense and they are one of forms of mycoplasma's persistence in human organism.
Sujet(s)
Infections à Mycoplasma/microbiologie , Mycoplasma/isolement et purification , Infections urinaires/diagnostic , Animaux , Antigènes bactériens/sang , ADN bactérien/sang , Femelle , Humains , Mâle , Souris , Mycoplasma/classification , Infections à Mycoplasma/diagnostic , ARN bactérien/sang , Infections urinaires/microbiologie , Frottis vaginauxRÉSUMÉ
AIM: To study the possibility of existence of antigenemia during urogenital mycoplasmal infections by detection the antigens of agents in blood and viscera of infected animals. MATERIALS AND METHODS: Rabbits and mice were intraperitoneally inoculated with Mycoplasma hominis and Ureaplasma urealyticum, their antigens and DNAs. Samples of blood and visceral organs were studied by several methods: cultural with use of standard media, PCR, RT-PCR, indirect hemagglutination test, and immunofluorescence assay for detection of antibodies. RESULTS: Bacteremia with M. hominis develops during 2 months after inoculation in rabbits and 3 weeks after inoculation in mice. Antigens of M. hominis and U. urealyticum were detected in serum and visceral organs significantly frequently than live cells and DNAs. Prolonged preservation of the antigens in animals' blood and viscera after intraperitoneal administration of "pure" antigens points to the presence of true mycoplasmal antigenemia. Forms of existence of antigens in organism are different-they can represent corpuscular antigens as well as soluble molecular compounds circulating in blood both in free state and in structure of immune complexes. Antigens as well as live cells are preserved in all studied organs. CONCLUSION: Inoculation of rabbits and mice with M. hominis or U. urealyticum resulted in development of generalized infection with persistence of the agent in all studied organs during initial phase of infection and predominant persistence in organs of immunogenesis during later phases.
Sujet(s)
Infections à Mycoplasma/immunologie , Infections à Mycoplasma/microbiologie , Mycoplasma hominis/pathogénicité , Infections à Ureaplasma/immunologie , Infections à Ureaplasma/microbiologie , Ureaplasma urealyticum/pathogénicité , Animaux , Anticorps antibactériens/sang , Antigènes bactériens/analyse , Antigènes bactériens/sang , Numération de colonies microbiennes , ADN bactérien/analyse , Tests d'hémagglutination , Humains , Immunisation , Souris , Infections à Mycoplasma/sang , Mycoplasma hominis/génétique , Mycoplasma hominis/immunologie , Lapins , Facteurs temps , Infections à Ureaplasma/sang , Ureaplasma urealyticum/génétique , Ureaplasma urealyticum/immunologieRÉSUMÉ
AIM: To study the time of preservation of antigens and DNA of urogenital mycoplasmas in circulating immune complexes (CIC) in blood of rabbits after single inoculation. MATERIALS AND METHODS: Rabbits were inoculated with Mycoplasma hominis and Ureaplasma urealiticum cell cultures washed in fetal calf serum. Reaction of aggregate-hemagglutination, immunofluorescence assay and PCR were used for detection of mycoplasmas' antigens and DNA. RESULTS: It was shown that DNA and antigens of M. hominis persist in free state and in structure of CIC during 1 month and 3 months respectively. In immunized rabbits antigens and DNA of mycoplasmas were detected in CIC structure even 6 months after the last immunization. Pattern of detection of DNA and antigens of U. urealyticum in blood of inoculated rabbits consists in that both DNA and antigens of the microorganism were detected in structure of CIC in blood samples during 70 days, whereas in free state they were detected only during 35 days. Incomplete elimination of CIC is possibly related to their small size (11S and lower) that allows them to circulate for a long time. CONCLUSION: Prolonged persistence of antigens and DNA of mycoplasmas in CIC structure is a fact that requires refinement of diagnostic criteria used for control of effectiveness of etiotropic therapy.
Sujet(s)
Complexe antigène-anticorps/sang , Antigènes bactériens/sang , ADN bactérien/sang , Infections à Mycoplasma/sang , Mycoplasma hominis/immunologie , Infections à Ureaplasma/sang , Ureaplasma/immunologie , Animaux , Anticorps antibactériens/sang , Diagnostic différentiel , Tests d'hémagglutination , Humains , Infections à Mycoplasma/diagnostic , Infections à Mycoplasma/immunologie , Mycoplasma hominis/génétique , Réaction de polymérisation en chaîne , Lapins , Sensibilité et spécificité , Ureaplasma/génétique , Infections à Ureaplasma/diagnostic , Infections à Ureaplasma/immunologieRÉSUMÉ
Exogenous NO donor 3,3-bis-(nitroxymethyl)oxetane (NMO) was synthesized at the Institute for Problems of Chemical Physics (Russian Academy of Sciences). This compound was shown to inhibit cell death (apoptosis and necrosis) in cyclophosphamide-sensitive and cyclophosphamide-resistant P388 murine tumor. p53 protein was expressed in both lines of tumor cells. NO donor NMO had little effect on p53 protein expression in cells of both stains. Our results suggest that the proapoptotic effect of NMO is mediated by the p53-independent molecular mechanisms.
Sujet(s)
Apoptose/effets des médicaments et des substances chimiques , Éthers cycliques/pharmacologie , Leucémie P388/traitement médicamenteux , Donneur d'oxyde nitrique/pharmacologie , Protéine p53 suppresseur de tumeur/métabolisme , Animaux , Antinéoplasiques alcoylants/pharmacologie , Apoptose/physiologie , Numération cellulaire , Lignée cellulaire tumorale , Cyclophosphamide/pharmacologie , Résistance aux médicaments antinéoplasiques , Leucémie P388/anatomopathologie , Leucémie P388/physiopathologie , Souris , Nécrose/traitement médicamenteux , Nécrose/physiopathologie , Facteurs tempsRÉSUMÉ
How long the viable cells of M. hominis, Ureaplasma spp., U. urealyticum, U. parvum, and their antigens retained in human serum at 37 degrees C was investigated. M. hominis cells were shown to hold their viability within 12 days with a gradual titer drop, the antigens being also detected within 12 days whereas intracellular and extracellular DNAs were seen within 40 days (an observation time). Under the same conditions, Ureaplasma cells died after 24 hours, their antigens were disrupted following 3 days and intracellular and extracellular DNAs of different species were detectable by polymerase chain reaction (PCR) within 17-40 days. The long preservation of extracellular and dead cell DNAs suggests that diagnostic examination of patients by means of PCR may yield false-positive results.
Sujet(s)
Antigènes bactériens/sang , ADN bactérien/sang , Mycoplasma hominis/cytologie , Ureaplasma/cytologie , Techniques bactériologiques , Humains , Viabilité microbienne , Sérum , TempératureRÉSUMÉ
Exogenous NO donor 3,3-bis-(nitroxymethyl)oxetane (NMO) was synthesized at the Institute for Problems of Chemical Physics (Russian Academy of Sciences). This compound was shown to inhibit Ca2+-ATPase isolated from normal muscular cells and tumor cells. Both hydrolytic and transport functions of the enzyme were inhibited under these conditions. These changes were probably related to changes in membrane structure caused by NO donor. Our results suggest that changes in intracellular Ca2+ concentration can modulate the formation of tumor drug resistance.
Sujet(s)
Calcium-Transporting ATPases/antagonistes et inhibiteurs , Réticulum endoplasmique/effets des médicaments et des substances chimiques , Éthers cycliques/pharmacologie , Éthers cycliques/pharmacocinétique , Donneur d'oxyde nitrique/pharmacologie , Réticulum sarcoplasmique/effets des médicaments et des substances chimiques , Adénosine triphosphate/métabolisme , Animaux , Calcium/métabolisme , Calcium-Transporting ATPases/métabolisme , Relation dose-effet des médicaments , Réticulum endoplasmique/enzymologie , Éthers cycliques/synthèse chimique , Hydrolyse/effets des médicaments et des substances chimiques , Leucémie P388/enzymologie , Leucémie P388/anatomopathologie , Souris , Muscles/effets des médicaments et des substances chimiques , Muscles/enzymologie , Pyrènes , Lapins , Réticulum sarcoplasmique/enzymologie , Spectrométrie de fluorescenceRÉSUMÉ
In monkeys contained in captivity conditions in open-air cages or in group cages human mycoplasmas are often detected: antigens of Mycoplasma hominis in blood serum were revealed in 33.3% of cases, and antibodies to it--in 15.6% of cases. IgM to M. hominis were detected more often than IgG. In 8 monkeys both types of immunoglobulins were detected. Rates of detection of Ureaplasma urealyticum antigens and specific antibodies were 43.1% and 31.1% respectively, and IgG were found more frequently than IgM (in 22 cases both types of immunoglobulins were revealed). High rates of M. hominis and U. urealyticum antigens and antibodies detection in blood serum of both healthy monkeys and monkeys with urogenital tract diseases show prevalence of human mycoplasmas carriage among monkeys contained in captivity conditions.