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1.
Rev. peru. med. exp. salud publica ; 39(3): 372-375, jul.-sep. 2022. tab
Article de Espagnol | LILACS, LIPECS | ID: biblio-1410013

RÉSUMÉ

RESUMEN Saprochaete capitata es una causa rara de infección fúngica invasiva en pacientes inmunocomprometidos con alta mortalidad y resistencia antifúngica. Presentamos el caso de un niño de cinco años con diagnóstico de aplasia medular, sometido a trasplante de progenitores hematopoyéticos (TPH), que cursó con neutropenia febril persistente, dolor abdominal intenso, aparición de lesiones maculopapulares en piel y deterioro de la función renal. Se identificó la presencia de S. capitata, en hemocultivos transcatéter venoso central. Esta infección fúngica invasiva resulta ser rara, pero emergente y potencialmente mortal, en pacientes con neutropenia febril persistente y uso prolongado de dispositivos invasivos intravasculares como catéter venoso central.


ABSTRACT Saprochaete capitata is a rare cause of invasive fungal infection in immunocompromised patients with high mortality and antifungal resistance. We present the case of a 5-year-old boy with bone marrow aplasia, who underwent hematopoietic stem cell transplantation (HSCT) and presented persistent febrile neutropenia, abdominal pain, appearance of maculopapular lesions on the skin, and impaired renal function. The presence of S. capitata was identified by blood culture from a central venous catheter. This invasive fungal infection is rare but emergent and life-threatening, especially in immunocompromised patients with persistent febrile neutropenia and prolonged use of invasive devices such as central venous catheters.


Sujet(s)
Humains , Mâle , Enfant d'âge préscolaire , Sujet immunodéprimé , Infections fongiques invasives/microbiologie , Géotrichose/microbiologie , Geotrichum/isolement et purification , Anémie aplasique/complications , Issue fatale , Infections fongiques invasives/traitement médicamenteux , Géotrichose/traitement médicamenteux , Antifongiques/usage thérapeutique
2.
J Clin Microbiol ; 60(1): e0160721, 2022 01 19.
Article de Anglais | MEDLINE | ID: mdl-34669454

RÉSUMÉ

Magnusiomyces and Geotrichum species are ascomycetous yeasts that can cause potentially life-threatening invasive fungal infections commonly referred to as geotrichosis. In this study, we aimed to estimate the incidence and mortality of these infections in a German tertiary care center. Furthermore, we evaluated the suitability of the fungal biomarkers galactomannan (GM) and ß-1,3-d-glucan (BDG), which are both recommended as surrogate markers for Magnusiomyces capitatus infection by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) and the European Confederation of Medical Mycology (ECMM) joint clinical guidelines for the diagnosis and management of rare invasive yeast infections for detection of invasive geotrichosis. Cases meeting the inclusion criteria for invasive Magnusiomyces/Geotrichum infection were retrospectively identified. Serum samples and culture supernatants were analyzed with two commercially available fungal antigen tests (Platelia Aspergillus Ag EIA and Wako ß-glucan test). For a control cohort, outpatient samples sent for lues testing were included. Thirty-eight cases of Magnusiomyces/Geotrichum infection were identified over an 11-year observation period. In the majority of cases, the fungus was isolated from intra-abdominal specimens of patients with a history of abdominal surgery/procedures (n = 32). All cases of fungemia occurred exclusively in haemato-oncologic patients (n = 14). Thirty-day survival was 42% in the fungemia and 43% in the intra-abdominal geotrichosis group. Serum samples were available for 23 patients (14 bloodstream and nine intra-abdominal infections). While BDG sensitivity was 65%, none of the sera was GM positive. This finding was supported by in vitro experiments analyzing fungal culture supernatants: M. capitatus secretes significant amounts of BDG but not GM. Specificity was 96% for BDG and 100% for GM. Magnusiomyces and Geotrichum infections are not limited to haemato-oncologic patients. Contrasting the current ESCMID/ECMM recommendation, our results indicate that GM is no suitable biomarker for the diagnosis of Magnusiomyces infection. Contrarily, BDG sensitivity is comparable to that of candidemia.


Sujet(s)
Géotrichose , Geotrichum , Infections fongiques invasives , Mannanes , Protéoglycanes , Saccharomycetales , bêta-Glucanes , Marqueurs biologiques/sang , Galactose/analogues et dérivés , Géotrichose/sang , Géotrichose/diagnostic , Geotrichum/isolement et purification , Humains , Infections fongiques invasives/sang , Infections fongiques invasives/diagnostic , Mannanes/sang , Protéoglycanes/sang , Études rétrospectives , Saccharomycetales/isolement et purification , Sensibilité et spécificité , bêta-Glucanes/sang
3.
Int J Food Microbiol ; 345: 109130, 2021 May 02.
Article de Anglais | MEDLINE | ID: mdl-33735781

RÉSUMÉ

Pélardon is an artisanal French raw goat's milk cheese, produced using natural whey as a backslop. The aim of this study was to identify key microbial players involved in the acidification and aroma production of this Protected Designation of Origin cheese. Microbial diversity of samples, collected from the raw milk to 3-month cheese ripening, was determined by culture-dependent (MALDI-TOF analysis of 2877 isolates) and -independent (ITS2 and 16S metabarcoding) approaches and linked to changes in biochemical profiles (volatile compounds and acids). In parallel, potential dominant autochthonous microorganism reservoirs were also investigated by sampling the cheese-factory environment. Complex and increasing microbial diversity was observed by both approaches during ripening although major discrepancies were observed regarding Lactococcus lactis and Lacticaseibacillus paracasei fate. By correlating microbial shifts to biochemical changes, Lactococcus lactis was identified as the main acidifying bacterium, while L. mesenteroides and Geotrichum candidum were prevalent and associated with amino acids catabolism after the acidification step. The three species were dominant in the whey (backslop). In contrast, L. paracasei, Enterococcus faecalis, Penicillium commune and Scopulariopsis brevicaulis, which dominated during ripening, likely originated from the cheese-making environment. All these four species were positively correlated to major volatile compounds responsible for the goaty and earthy Pélardon cheese aroma. Overall, this work highlighted the power of MALDI-TOF and molecular techniques combined with volatilome analyses to dynamically follow and identify microbial communities during cheese-making and successively identify the key-players involved in aroma production and contributing to the typicity of Pélardon cheese.


Sujet(s)
Bactéries/classification , Bactéries/métabolisme , Fromage/microbiologie , Champignons/classification , Champignons/métabolisme , Lait/microbiologie , Animaux , Bactéries/isolement et purification , Enterococcus faecalis/isolement et purification , Enterococcus faecalis/métabolisme , Champignons/isolement et purification , Geotrichum/isolement et purification , Geotrichum/métabolisme , Capra , Lacticaseibacillus paracasei/isolement et purification , Lacticaseibacillus paracasei/métabolisme , Lactococcus lactis/isolement et purification , Lactococcus lactis/métabolisme , Microbiote , Odorisants/analyse , Penicillium/isolement et purification , Penicillium/métabolisme , Scopulariopsis/isolement et purification , Scopulariopsis/métabolisme , Spectrométrie de masse MALDI
4.
Chemosphere ; 259: 127507, 2020 Nov.
Article de Anglais | MEDLINE | ID: mdl-32650171

RÉSUMÉ

In this study, a yeast-like fungal strain (LG-8), newly isolated from spontaneous Tibet kefir in China, was identified as Geotrichum candidum on the basis of its morphological characteristics and ITS5.8S gene sequence. Interestingly, the strain was able to remove more than 99% of Pb2+ ions in water at low concentrations and a maximum of 325.68 mg lead/g of dry biomass. The results of selective passivation experiments suggested that phosphate, amide and carboxyl groups on the cell wall contributed to lead removal. Scanning electron microscopy (SEM) photomicrographs revealed that large amounts of micro/nanoparticles formed on the cell wall, and energy dispersive X-ray spectroscopy (EDX) results further indicated the presence of lead along with phosphorus and chlorine in the particles. Furthermore, the results of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses revealed that the particles were composed of pyromorphite [Pb5(PO4)3Cl], a highly insoluble lead mineral. Importantly, this is the first time that the biomineralization of lead into pyromorphite has been observed as the major mechanism for lead removal by G. candidum LG-8, providing a new strategy to scavenge heavy metals from aquatic environment in an eco-friendly manner.


Sujet(s)
Geotrichum/isolement et purification , Kéfir/microbiologie , Plomb/isolement et purification , Lait/microbiologie , Purification de l'eau/méthodes , Animaux , Biominéralisation , Geotrichum/métabolisme , Microscopie électronique à balayage , Minéraux/composition chimique , Phosphates/composition chimique , Analyse spectrale , Tibet
5.
J Dairy Sci ; 103(5): 4056-4067, 2020 May.
Article de Anglais | MEDLINE | ID: mdl-32173014

RÉSUMÉ

Robiola di Roccaverano, from the Piedmont region of Italy, is a Protected Designation of Origin soft cheese made with raw goat milk. The peculiarity of this cheese is that during the manufacturing process, a natural starter culture (NC) is added to raw milk. This study examined the viable microorganisms of technological interest, including lactic acid bacteria and fungal populations, in samples of raw milk, NC, and fresh and ripened cheese collected from one dairy using culture-dependent techniques. First, the isolated colonies were analyzed using random amplification of polymorphic DNA (RAPD) PCR, and strains with similar fingerprints were clustered together. Further, representative isolates of each group were subjected to 16S or 26S ribosomal DNA sequencing. Finally, species-specific PCR was conducted to distinguish the Lactococcus lactis ssp. lactis and Lc. lactis ssp. cremoris. Among the studied lactic acid bacteria, 13 RAPD profiles were obtained, corresponding to 9 different bacterial species or subspecies. Concerning mold and yeast isolates, 5 species were found that coincided with 5 RAPD types. Observing the strains isolated in the study, Lc. lactis was the most prevalent species in raw milk and NC samples, and Leuconostoc mesenteroides was the predominant species identified in 5- and 15-d cheese isolates. Furthermore, whereas only these 2 species were detected in NC, Enterococcus and Lactobacillus genera were found in raw milk and cheese, respectively. Concerning the mold and yeast isolates, in NC Kluyveromyces spp. was mainly found, and in cheese samples the representative species were Geotrichum candidum and Yarrowia lipolytica. Finally, raw milk and cheese safety were evaluated, and the samples complied with the standard required by European Commission regulation number 2073/2005.


Sujet(s)
Bactéries/isolement et purification , Fromage/microbiologie , Enterococcus/isolement et purification , Lactobacillus/isolement et purification , Animaux , Bactéries/classification , Enterococcus/classification , Microbiologie alimentaire , Geotrichum/classification , Geotrichum/isolement et purification , Capra , Kluyveromyces/classification , Kluyveromyces/isolement et purification , Lait/microbiologie , Typage moléculaire , Technique RAPD , Levures/classification , Levures/isolement et purification
6.
Int J Food Microbiol ; 319: 108503, 2020 Apr 16.
Article de Anglais | MEDLINE | ID: mdl-31923767

RÉSUMÉ

Thirty nine strains of Galactomyces geotrichum molds were isolated from a traditional fried cottage cheese and production of polyunsaturated fatty acids (PUFA) was assessed. Among them eleven strains produced an extracellular lipids enriched in n-6 and n-3 PUFA. The extracellular lipids produced by G. geotrichum strain 38 contained the highest amounts of total PUFA (24.3%), with the highest contribution of n-3 fatty acids (17.9%), where α-linolenic, eicosapentaenoic, docosapentaenoic and docosahexaenoic acids were the main contributors. To obtain maximal production of PUFA, composition of the medium consisted of 10 g/L rapeseed oil, 5 g/L yeast extract, 0.05 g/L K2HPO4, 0.17 g/L MgSO4, 0.015 g/L MnSO4, 0.015 g/L ZnSO4, 0.05 g/L FeSO4, and 10 mg/L vitamin B12. The optimal growth conditions at 30 °C involve: aeration at 1.5 vvm (volume of air per volume of broth per minute) at pH 6.5. The cheese produced under described conditions contained higher amount of n-3 PUFA (0.25 mg/g cheese) in comparison to control (0.01 mg/g). α-Linolenic acid predominated among n-3 fatty acids. Galactomyces geotrichum is a natural microflora of dairy products, and could be used to enrich food/cheese in deficient omega-3 lipids.


Sujet(s)
Fromage/microbiologie , Acides gras omega-3/biosynthèse , Geotrichum/métabolisme , Animaux , Acides gras omega-6/biosynthèse , Geotrichum/classification , Geotrichum/isolement et purification
7.
Toxins (Basel) ; 11(8)2019 08 06.
Article de Anglais | MEDLINE | ID: mdl-31390769

RÉSUMÉ

Citrus are vulnerable to the postharvest decay caused by Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, which are responsible for the green mold, blue mold, and sour rot post-harvest disease, respectively. The widespread economic losses in citriculture caused by these phytopathogens are minimized with the use of synthetic fungicides such as imazalil, thiabendazole, pyrimethanil, and fludioxonil, which are mainly employed as control agents and may have harmful effects on human health and environment. To date, numerous non-chemical postharvest treatments have been investigated for the control of these pathogens. Several studies demonstrated that biological control using microbial antagonists and natural products can be effective in controlling postharvest diseases in citrus, as well as the most used commercial fungicides. Therefore, microbial agents represent a considerably safer and low toxicity alternative to synthetic fungicides. In the present review, these biological control strategies as alternative to the chemical fungicides are summarized here and new challenges regarding the development of shelf-stable formulated biocontrol products are also discussed.


Sujet(s)
Citrus/microbiologie , Geotrichum/effets des médicaments et des substances chimiques , Penicillium/effets des médicaments et des substances chimiques , Lutte biologique contre les nuisibles , Fongicides industriels/pharmacologie , Geotrichum/isolement et purification , Penicillium/isolement et purification
9.
Biomolecules ; 9(4)2019 03 29.
Article de Anglais | MEDLINE | ID: mdl-30934892

RÉSUMÉ

To prevent citrus decay caused by Geotrichum citri-aurantii, 12 natural products were isolated from two endophytic fungi, in which cytosporone B was shown to have excellent bioactivity for control of G. citri-aurantii with median effect concentration (EC50) of 26.11 µg/mL and minimum inhibitory concentration (MIC) of 105 µg/mL, and also significantly reduced the decay of sugar orange during the in vivo trials. In addition, cytosporone B could alter the morphology of G. citri-aurantii by causing distortion of the mycelia and loss of membrane integrity. Differentially expressed genes (DEGs) between cytosporone B-treated and -untreated samples were revealed by Illumina sequencing, including 3540 unigenes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that most DEGs were related to metabolic production and cell membrane. These findings suggest cytosporone B is a promising biological preservative to control citrus decay and reveal the action mechanism of cytosporone B in relation to the destruction of the fungal cell membrane at both morphological and molecular levels.


Sujet(s)
Antifongiques/pharmacologie , Geotrichum/effets des médicaments et des substances chimiques , Phénylacétates/pharmacologie , Antifongiques/composition chimique , Antifongiques/isolement et purification , Relation dose-effet des médicaments , Geotrichum/isolement et purification , Tests de sensibilité microbienne , Structure moléculaire , Phénylacétates/composition chimique , Phénylacétates/isolement et purification , Relation structure-activité
10.
Ecotoxicol Environ Saf ; 171: 443-450, 2019 Apr 30.
Article de Anglais | MEDLINE | ID: mdl-30639870

RÉSUMÉ

Viable and metabolically active fungi in toxic mixed liquors, treating landfill leachates and municipal wastewaters, were identified by culture depending methods. A selective culture medium consisting of wastewater and agar (WA) restrained fungi that could be randomly present (94% of the 51 taxa retrieved on WA were sample-specific), overcoming the problem of fast growing fungi or mycoparasite fungi. Moreover, WA allowed the isolation of fungi with a possible role in the degradation of pollutants typically present in the two wastewaters. Phoma medicaginis var. medicaginis, Chaetomium globosum, and Geotrichum candidum were mainly found in municipal wastewater, whereas Pseudallescheria boydii, Scedosporium apiospermum, Aspergillus pseudodeflectus, and Scopulariopsis brevicaulis were typical of landfill leachate.


Sujet(s)
Champignons/isolement et purification , Eaux usées/microbiologie , Polluants chimiques de l'eau/analyse , Agar-agar/composition chimique , Ascomycota/isolement et purification , Aspergillus/isolement et purification , Chaetomium/isolement et purification , Numération de colonies microbiennes , Surveillance de l'environnement , Champignons/classification , Geotrichum/isolement et purification , Concentration en ions d'hydrogène , Pseudallescheria/isolement et purification , Scedosporium/isolement et purification , Scopulariopsis/isolement et purification
11.
FEMS Yeast Res ; 19(1)2019 01 01.
Article de Anglais | MEDLINE | ID: mdl-30295727

RÉSUMÉ

Geotrichum candidum is a fungus-like yeast widely used as a starter culture for cheese ripening for its proteolytic and lipolytic activities and its contribution to the cheese flavours. The sequenced strain G. candidum CLIB 918 was isolated from cheese Pont-L'Evêque. This strain's ability to produce volatile compounds was compared to the ability of a known strong sulphur compound producer G. candidum strain (Gc203). The aminotransferase-coding genes BAT2 and ARO8 were identified to be involved in methionine catabolism. The production of volatile compounds indicated that the sequenced strain was a moderate producer compared to the strong producer strain. The major volatile compounds were produced from sulphur amino acid, branched-chain amino acid and fatty acid metabolisms. Metabolite content of the cells showed that the ability of the strain to produce volatile compounds was inversely proportional to its ability to store amino acids inside the cells. Reduced glutathione, hypotaurine and taurine intracellular concentrations and volatile fatty aldehyde production indicated the role of oxidative stress sensitivity in flavour production. The increase in expression of several genes in a Reblochon-type cheese at the end of ripening confirmed that oxygen and iron were key factors regulating cheese flavour production.


Sujet(s)
Acides aminés/métabolisme , Aromatisants/métabolisme , Régulation de l'expression des gènes fongiques , Geotrichum/génétique , Geotrichum/métabolisme , Stress oxydatif , Composés organiques volatils/métabolisme , Fromage/microbiologie , Geotrichum/isolement et purification , Voies et réseaux métaboliques/génétique , Séquençage du génome entier
12.
Jpn J Infect Dis ; 72(2): 130-132, 2019 Mar 25.
Article de Anglais | MEDLINE | ID: mdl-30381692

RÉSUMÉ

Invasive fungal infections are one of the vital complications among acute leukemia patients undergoing induction chemotherapy. Among them, Geotrichum clavatum infections present extremely rarely with atypical clinical symptoms which make them difficult to diagnose. In this paper, we report a case of infection caused by Geotrichum clavatum in a 10-year old child with acute leukemia, which is the first documented case from mainland China. With underlying childhood leukemia, the child suffered from recurrent bacterial and fungal infection and even underwent abdominal surgery during the treatment. Fortunately, the therapeutic effect was finally achieved by adjusting the treatment program to dual anti-fungal treatment with micafungin and amphotericin B. Information regarding the epidemiological, clinical, and therapeutic features, in this case, shows significant perspectives for anti-fungal treatment for immunocompromised individuals, wherefore the rate of recovery and survival can be achieved.


Sujet(s)
Géotrichose/diagnostic , Géotrichose/anatomopathologie , Geotrichum/isolement et purification , Infections fongiques invasives/étiologie , Infections fongiques invasives/anatomopathologie , Leucémies/complications , Amphotéricine B/administration et posologie , Antifongiques/administration et posologie , Enfant , Chine , Géotrichose/traitement médicamenteux , Humains , Infections fongiques invasives/traitement médicamenteux , Mâle , Micafungine/administration et posologie , Résultat thérapeutique
13.
Int J Mol Sci ; 19(7)2018 07 07.
Article de Anglais | MEDLINE | ID: mdl-29986518

RÉSUMÉ

Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-ß-picrylhydrazyl (DPPH), ß-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography­High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography­Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.


Sujet(s)
Protéines fongiques/pharmacologie , Geotrichum/isolement et purification , Penicillium/isolement et purification , Phoeniceae/microbiologie , Antibactériens/métabolisme , Antibactériens/pharmacologie , Antifongiques/métabolisme , Antifongiques/pharmacologie , Endophytes/composition chimique , Endophytes/isolement et purification , Endophytes/métabolisme , Protéines fongiques/métabolisme , Geotrichum/composition chimique , Geotrichum/métabolisme , Bactéries à Gram négatif/effets des médicaments et des substances chimiques , Bactéries à Gram positif/effets des médicaments et des substances chimiques , Cellules HepG2 , Humains , Tests de sensibilité microbienne , Viabilité microbienne/effets des médicaments et des substances chimiques , Penicillium/composition chimique , Penicillium/métabolisme , Maladies des plantes/microbiologie , Racines de plante/microbiologie , Composés organiques volatils/composition chimique , Composés organiques volatils/isolement et purification , Composés organiques volatils/pharmacologie
14.
J Mycol Med ; 28(2): 387-389, 2018 Jun.
Article de Français | MEDLINE | ID: mdl-29709266

RÉSUMÉ

We are reporting the case of an 82-year-old Yemeni patient, renal transplant recipient who was admitted to our institution and who subsequently developed disseminated infection with Saprochaete capitata. This pathogenic fungus is rarely reported in patients with solid organ trans-plants. Saprochaete capitata is an emerging fungal pathogen, ubiquitously spread in the environment. This is the second case to our knowledge of infection with Saprochaete capitata in a renal transplant patient. Our patient was treated for multiple nosocomial infections with prolonged antibiotic courses. He succumbed to the infection with Saprochaete capitate after several weeks spent in the intensive care unit.


Sujet(s)
Infection croisée/microbiologie , Géotrichose/microbiologie , Geotrichum/isolement et purification , Transplantation rénale/effets indésirables , Sujet âgé de 80 ans ou plus , Antifongiques/usage thérapeutique , Infection croisée/traitement médicamenteux , Échinocandines/usage thérapeutique , Issue fatale , Fongémie/traitement médicamenteux , Fongémie/microbiologie , Géotrichose/sang , Géotrichose/traitement médicamenteux , Geotrichum/pathogénicité , Humains , Unités de soins intensifs , Mâle , Receveurs de transplantation
15.
Article de Anglais | MEDLINE | ID: mdl-29229638

RÉSUMÉ

Saprochaete capitata, formerly known as Geotrichum capitatum, is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the S. capitata hot spot 1 region of the FKS gene (FKS HS1), which codifies for the catalytic subunit of ß-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the FKS1 HS1 segment of 12 different fungal species. Interestingly, analysis of the translated FKS HS1 sequences of 12 isolates of S. capitata revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.


Sujet(s)
Antifongiques/pharmacologie , Résistance des champignons aux médicaments/génétique , Échinocandines/pharmacologie , Protéines fongiques/génétique , Régulation de l'expression des gènes fongiques , Geotrichum/génétique , Glucosyltransferases/génétique , Substitution d'acide aminé , Séquence nucléotidique , ADN fongique/génétique , Protéines fongiques/métabolisme , Géotrichose/traitement médicamenteux , Géotrichose/microbiologie , Géotrichose/anatomopathologie , Geotrichum/effets des médicaments et des substances chimiques , Geotrichum/croissance et développement , Geotrichum/isolement et purification , Glucosyltransferases/métabolisme , Humains , Tests de sensibilité microbienne , Sous-unités de protéines/génétique , Sous-unités de protéines/métabolisme , Analyse de séquence d'ADN
16.
Microb Ecol ; 75(3): 647-661, 2018 Apr.
Article de Anglais | MEDLINE | ID: mdl-28894891

RÉSUMÉ

Plant-associated endophytes are recognized as sources of novel bioactive molecules having diverse applications. In this study, an endophytic yeast-like fungal strain was isolated from the fruit of eggplant (Solanum melongena) and identified as Geotrichum candidum through phenotypic and genotypic characterizations. This endophytic G. candidum isolate PF005 was found to emit fruity scented volatiles. The compositional profiling of volatile organic compounds (VOCs) revealed the presence of 3-methyl-1-butanol, ethyl 3-methylbutanoate, 2-phenylethanol, isopentyl acetate, naphthalene, and isobutyl acetate in significant proportion when analyzed on a time-course basis. The VOCs from G. candidum exhibited significant mycelial growth inhibition (54%) of phytopathogen Rhizoctonia solani, besides having mild antifungal activity against a few other fungi. The source of carbon as a nutrient was found to be an important factor for the enhanced biosynthesis of antifungal VOCs. The antifungal activity against phytopathogen R. solani was improved up to 91% by feeding the G. candidum with selective precursors of alcohol and ester volatiles. Furthermore, the antifungal activity of VOCs was enhanced synergistically up to 92% upon the exogenous addition of naphthalene (1.0 mg/plate). This is the first report of G. candidum as an endophyte emitting antifungal VOCs, wherein 2-penylethanol, isopentyl acetate, and naphthalene were identified as important contributors to its antifungal activity. Possible utilization of G. candidum PF005 as a mycofumigant has been discussed based upon its antifungal activity and the qualified presumption of safety status.


Sujet(s)
Antifongiques/pharmacologie , Endophytes/métabolisme , Geotrichum/métabolisme , Solanum melongena/microbiologie , Composés organiques volatils/pharmacologie , Antifongiques/composition chimique , Carbone/métabolisme , Milieux de culture/composition chimique , ADN fongique/génétique , Endophytes/génétique , Endophytes/croissance et développement , Endophytes/isolement et purification , Génotype , Geotrichum/génétique , Geotrichum/croissance et développement , Geotrichum/isolement et purification , Tests de sensibilité microbienne , Mycelium/effets des médicaments et des substances chimiques , Mycelium/croissance et développement , Maladies des plantes/microbiologie , Maladies des plantes/prévention et contrôle , ARN ribosomique 18S/génétique , Rhizoctonia/effets des médicaments et des substances chimiques , Rhizoctonia/croissance et développement , Rhizoctonia/pathogénicité , Composés organiques volatils/composition chimique
17.
Mycoses ; 60(4): 273-279, 2017 Apr.
Article de Anglais | MEDLINE | ID: mdl-28150341

RÉSUMÉ

Saprochaete and Geotrichum spp. are rare emerging fungi causing invasive fungal diseases in immunosuppressed patients and scarce evidence is available for treatment decisions. Among 505 cases of rare IFD from the FungiScope™ registry, we identified 23 cases of invasive infections caused by these fungi reported from 10 countries over a 12-year period. All cases were adults and previous chemotherapy with associated neutropenia was the most common co-morbidity. Fungaemia was confirmed in 14 (61%) cases and deep organ involvement included lungs, liver, spleen, central nervous system and kidneys. Fungi were S. capitata (n=14), S. clavata (n=5), G. candidum (n=2) and Geotrichum spp. (n=2). Susceptibility was tested in 16 (70%) isolates. All S. capitata and S. clavata isolates with the exception of one S. capitata (MIC 4 mg/L) isolate had MICs>32 mg/L for caspofungin. For micafungin and anidulafungin, MICs varied between 0.25 and >32 mg/L. One case was diagnosed postmortem, 22 patients received targeted treatment, with voriconazole as the most frequent first line drug. Overall mortality was 65% (n=15). Initial echinocandin treatment was associated with worse outcome at day 30 when compared to treatment with other antifungals (amphotericin B ± flucytosine, voriconazole, fluconazole and itraconazole) (P=.036). Echinocandins are not an option for these infections.


Sujet(s)
Géotrichose/microbiologie , Geotrichum/isolement et purification , Infections fongiques invasives/microbiologie , Enregistrements , Saccharomycetales/isolement et purification , Adolescent , Adulte , Sujet âgé , Amphotéricine B/pharmacologie , Amphotéricine B/usage thérapeutique , Antifongiques/pharmacologie , Antifongiques/usage thérapeutique , Échinocandines/pharmacologie , Échinocandines/usage thérapeutique , Femelle , Fluconazole/pharmacologie , Fluconazole/usage thérapeutique , Fongémie/diagnostic , Fongémie/traitement médicamenteux , Fongémie/microbiologie , Géotrichose/traitement médicamenteux , Géotrichose/mortalité , Geotrichum/classification , Geotrichum/effets des médicaments et des substances chimiques , Geotrichum/génétique , Humains , Sujet immunodéprimé , Infections fongiques invasives/traitement médicamenteux , Infections fongiques invasives/mortalité , Lipopeptides/pharmacologie , Lipopeptides/usage thérapeutique , Mâle , Micafungine , Tests de sensibilité microbienne , Adulte d'âge moyen , Neutropénie/complications , Neutropénie/traitement médicamenteux , Neutropénie/microbiologie , Saccharomycetales/classification , Saccharomycetales/effets des médicaments et des substances chimiques , Saccharomycetales/génétique , Voriconazole/pharmacologie , Voriconazole/usage thérapeutique , Jeune adulte
18.
Environ Sci Pollut Res Int ; 23(20): 20680-20686, 2016 Oct.
Article de Anglais | MEDLINE | ID: mdl-27473614

RÉSUMÉ

The purpose of this study was to measure the culturable airborne fungi (CAF) concentrations in the underground subway stations of Seoul, Korea at two time points. This study measured the CAF concentrations in enclosed environments at 16 underground stations of the Seoul Metro in 2006 and 2013 and investigated the effects of various environmental factors, including the presence of platform screen doors, temperature, relative humidity, and number of passengers. CAF concentrations at the stations in 2006 were significantly higher than that at the same stations in 2013 (p < 0.001). Furthermore, there was a significant correlation between CAF concentration and relative humidity (r = 0.311, p < 0.05). Geotrichum and Penicillium were the predominant genera. The CAF concentrations in stations with an operating supply air were significantly higher than that in stations with no supply air (p < 0.001). Therefore, it is recommended that special attention be given to stations with clean supplied air to improve the indoor air quality of these subway stations.


Sujet(s)
Microbiologie de l'air , Geotrichum/isolement et purification , Penicillium/isolement et purification , Voies ferrées , Surveillance de l'environnement , Geotrichum/croissance et développement , Humains , Limite de détection , Penicillium/croissance et développement , Séoul
19.
Food Microbiol ; 53(Pt A): 30-40, 2016 Feb.
Article de Anglais | MEDLINE | ID: mdl-26611167

RÉSUMÉ

A mixture of nine microorganisms (six bacteria and three yeasts) from the microflora of surface-ripened cheeses were subjected to in vitro digestive stress in a three-compartment "dynamic gastrointestinal digester" (DIDGI). We studied the microorganisms (i) grown separately in culture medium only (ii) grown separately in culture medium and then mixed, (iii) grown separately in culture medium and then included in a rennet gel and (iv) grown together in smear-ripened cheese. The yeasts Geotrichum candidum, Kluyveromyces lactis and Debaryomyces hansenii, were strongly resistant to the whole DIDGI process (with a drop in viable cell counts of less than <1 log CFU mL(-1)) and there were no significant differences between lab cultures and cheese-grown cultures. Ripening bacteria such as Hafnia alvei survived gastric stress less well when grown in cheese (with no viable cells after 90 min of exposure of the cheese matrix, compared with 6 CFU mL(-1) in lab cultures). The ability of Corynebacterium casei and Staphylococcus equorum to withstand digestive stress was similar for cheese and pure culture conditions. When grow in a cheese matrix, Brevibacterium aurantiacum and Arthrobacter arilaitensis were clearly more sensitive to the overall digestive process than when grown in pure cultures. Lactococcus lactis displayed poorer survival in gastric and duodenal compartments when it had been grown in cheese. In vivo experiments in BALB/c mice agreed with the DIDGI experiments and confirmed the latter's reliability.


Sujet(s)
Phénomènes physiologiques bactériens , Fromage/microbiologie , Tube digestif/microbiologie , Levures/physiologie , Animaux , Brevibacterium/isolement et purification , Brevibacterium/physiologie , Simulation numérique , Corynebacterium/isolement et purification , Corynebacterium/physiologie , Digestion , Tube digestif/composition chimique , Geotrichum/isolement et purification , Geotrichum/physiologie , Hafnia alvei/isolement et purification , Hafnia alvei/métabolisme , Techniques in vitro , Lactococcus lactis/isolement et purification , Lactococcus lactis/physiologie , Souris , Viabilité microbienne/effets des médicaments et des substances chimiques , Reproductibilité des résultats , Saccharomycetales/isolement et purification , Saccharomycetales/physiologie , Levures/classification
20.
Infez Med ; 23(3): 270-4, 2015 Sep.
Article de Anglais | MEDLINE | ID: mdl-26397299

RÉSUMÉ

Geotrichum capitatum is a rare pathogen that causes opportunistic fungal infections in immunocompromised patients, particulary in patients with hematological malignancies. We report the case of a 72-year patient with polytrauma whose outcome was fatal. During his stay in the intensive care unit (ICU), he received a broad-spectrum antimicrobial therapy and underwent different invasive procedures. After becoming febrile on the 7th day of admission, two consecutive bronchoalveolar lavage (BAL) specimens were taken for microbiological analysis. The isolated species came as G. capitatum, that was identified using VITEK 2. Unfortunately, patient died before fungal identification, so the antifungal therapy wasn't administered. This case presentation emphasizes the importance of Geotrichum capitatum as an emerging fungal pathogen, as well as the significance of the predisponing factors that contributed to development of infection.


Sujet(s)
Geotrichum/isolement et purification , Polytraumatisme/complications , Mycoses/complications , Infections opportunistes , Infections de l'appareil respiratoire/microbiologie , Sujet âgé , Fibrillation auriculaire/complications , Issue fatale , Humains , Sujet immunodéprimé , Mâle , Mycoses/diagnostic , Infections de l'appareil respiratoire/diagnostic , Facteurs de risque
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