Gender Bias and Perceptions of the Nursing Profession in Croatia: A Cross-Sectional Study Comparing Patients and the General Population.

Background: The way the nursing profession is perceived plays a pivotal role in shaping the public's attitude toward its members, primarily influenced by interactions with nurses and media representation. However, it can be significantly impacted by historical and traditional stereotypes, coupled with enduring gender bias and other preconceived notions. Objectives: The study aimed to examined how the title "nurse" reflects the profession's identity, perception as a female occupation, and status as a healthcare activity. It also assessed awareness of nurses' roles, education and public presence, comparing general population and patients' opinions in the Republic of Croatia. Methods: Conducted as a cross-sectional study, this research employed a structured questionnaire and a convenience sampling approach. It encompassed both the general population and patients from Croatia, with data collection spanning from February 15, 2023, to March 16, 2023, and resulting in a total sample size of 624 participants. Statistical analysis included both descriptive and inferential techniques, and significance was set at p < .01 (two-tailed). Results: A substantial proportion of respondents, amounting to 38.1%, correlate the term "nurse" with the role of the physician's assistant, whereas only 49.5% associate it with a true professional functioning within their field of work. Furthermore, 73.7% study participants perceive the nursing profession as being desirable for men. Upon examining the perspectives of the general population and patients, statistically significant differences emerged in terms of their perceptions regarding the perceived work scope (p < .001) and the level of sufficient educational attainment (p < .001) of nursing professionals. Conclusion: The analysis uncovers varying perceptions of the nursing profession in the Republic of Croatia, as well as evolving perceptions of gender diversity within nursing roles. Differing viewpoints between the general population and patients in this study emphasize the need for tailored education and communication initiatives to improve the image of nursing.

Di-mannosylation enhances the adjuvant properties of adamantane-containing desmuramyl peptides in vivo.

Muramyl dipeptide (MDP) is the smallest essential peptidoglycan substructure capable of promoting both innate and adaptive immune responses. Herein, we report on the design, synthesis, and in vivo study of the adjuvant properties of two novel MDP analogs containing an achiral adamantyl moiety attached to the desmuramyl dipeptide (DMP) pharmacophore and additionally modified by one mannosyl subunit (derivative 7) or two mannosyl subunits (derivative 11). Mannose substructures were introduced in order to assess how the degree of mannosylation affects the immune response and nucleotide-binding oligomerization-domain-containing protein 2 (NOD2) binding affinity, compared to the reference compound ManAdDMP. Both mannosylated MDP analogs showed improved immunomodulating properties, while the di-mannosylated derivative 11 displayed the highest, statistically significant increase in anti-OVA IgG production. In this study, for the first time, the di-mannosylated DMP derivative was synthesized and immunologically evaluated. Derivative 11 stimulates a Th-2-polarized type of immune reaction, similar to the reference compound ManAdDMP and MDP. Molecular dynamics (MD) simulations demonstrate that 11 has a higher NOD2 binding affinity than 7, indicating that introducing the second mannose significantly contributes to the binding affinity. Mannose interacts with key amino acid residues from the LRR hydrophobic pocket of the NOD2 receptor and loop 2.

Mannose Ligands for Mannose Receptor Targeting.

The mannose receptor (MR, CD 206) is an endocytic receptor primarily expressed by macrophages and dendritic cells, which plays a critical role in both endocytosis and antigen processing and presentation. MR carbohydrate recognition domains (CRDs) exhibit a high binding affinity for branched and linear oligosaccharides. Furthermore, multivalent mannose presentation on the various templates like peptides, proteins, polymers, micelles, and dendrimers was proven to be a valuable approach for the selective and efficient delivery of various therapeutically active agents to MR. This review provides a detailed account of the most relevant and recent aspects of the synthesis and application of mannosylated bioactive formulations for MR-mediated delivery in treatments of cancer and other infectious diseases. It further highlights recent findings related to the necessary structural features of the mannose-containing ligands for successful binding to the MR.