RESUMO
The hemostatic activity of blood shows a circadian variation with a higher frequency of acute coronary events in the morning. The thrombotic tendency of blood is influenced by many factors, including platelets. Diurnal changes of in vivo platelet activation were investigated by whole blood flow cytometry in 10 young healthy male volunteers using anti-GMP-140 (anti-alpha-granule membrane protein 140 kD) monoclonal antibody at 3h intervals from 06:00 to 24:00. We also studied circulating platelet aggregates to investigate whether there exists a similarity between the results of these methods. Results of flow cytometric analysis indicate that there is an increase in platelet activation during the period from 06:00 to 09:00. Platelet activation then decreases gradually during the period from noon to midnight. These changes are accompanied by a similar trend in circulating platelet aggregates. This suggests that GMP-140 expression on platelets is synchronized with or followed by platelet aggregate formation in vivo, and increased platelet activation may predispose individuals to thrombosis at this time.
Assuntos
Anticorpos Monoclonais , Ritmo Circadiano/fisiologia , Selectina-P/imunologia , Ativação Plaquetária , Adulto , Citometria de Fluxo/métodos , Humanos , Masculino , Agregação Plaquetária , Complexo Glicoproteico GPIb-IX de Plaquetas/imunologiaRESUMO
UNLABELLED: The effect of interleukin-1 (IL-1) as an autocrine growth factor on the proliferation of the acute myeloblastic leukemia (AML) blasts was studied. Bone marrow specimens were obtained from nine patients with different subgroups of AML. IL-1 receptor antagonist (IL-1RA) and IL-1 ß neutralizing antibody (IL-1ß NA) alone or in combination were added to the culture mediums of the AML blast cultures for the detection of their inhibitory effect on AML blast cell proliferation and colony formation. Average colony numbers in the IL-RA, IL-ßNA, and IL-IRA plus IL-IßNA included culture flasks, were 63.7 ± 21.5 %, 69.5 ± 19 %, 53.4 ± 23.7 %, respectively, as compared to those of the control (p < 0.01). Inhibition of colony formation by IL-IRA plus IL-IßNA was more prominent than by IL-IßNA alone (p < 0.01). No correlation between the inhibition of AML blast colony ormation and FAB AML subgroups was seen. RESULT: Both IL-1RA or IL-IßNA or in combination induced varying degrees of inhibition on blast colony formation. IL-I inhibitory molecules could be considered as an alternative therapy for AML in patients whose blast cells are sensitive to IL-1 inhibition.
RESUMO
Two patients with paraneoplastic cerebellar degeneration accompanying Hodgkin's disease were treated with plasma exchange in combination with chemotherapy or radiation therapy, respectively. Significant improvement in neurologic symptoms was obtained in 1 of them.
Assuntos
Doenças Cerebelares/complicações , Doença de Hodgkin/complicações , Síndromes Paraneoplásicas/complicações , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica , Doenças Cerebelares/radioterapia , Doenças Cerebelares/terapia , Terapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndromes Paraneoplásicas/radioterapia , Síndromes Paraneoplásicas/terapia , Troca PlasmáticaRESUMO
A 60-year-old woman who presented with weakness, night sweats, bone pain, easy bruising and weight loss was found to have ecchymoses and hepatosplenomegaly. Blood counts showed persistent neutrophilia of mature cell type with Döhle bodies and toxic granulation. Coexistence of chronic neutrophilic leukemia and multiple myeloma of kappa light chain type was documented by bone marrow examination and immunofixation.
Assuntos
Cadeias kappa de Imunoglobulina/urina , Leucemia Neutrofílica Crônica/diagnóstico , Mieloma Múltiplo/diagnóstico , Neoplasias Primárias Múltiplas/diagnóstico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biópsia , Medula Óssea/patologia , Doxorrubicina/administração & dosagem , Etoposídeo/administração & dosagem , Feminino , Humanos , Hidroxiureia/administração & dosagem , Leucemia Neutrofílica Crônica/tratamento farmacológico , Leucemia Neutrofílica Crônica/patologia , Pessoa de Meia-Idade , Mieloma Múltiplo/tratamento farmacológico , Mieloma Múltiplo/patologia , Neoplasias Primárias Múltiplas/tratamento farmacológico , Neoplasias Primárias Múltiplas/patologiaRESUMO
A 27-year-old woman with Hodgkin's disease, nodular sclerasing type, having received two courses of radiation therapy with one year and nine months interval and a prolonged course of chemotherapy with combinations of COPP, MOPP and CVPP, developed oat cell carcinoma of the lung, four years after the initial diagnosis.
Assuntos
Carcinoma de Células Pequenas/complicações , Doença de Hodgkin/complicações , Neoplasias Pulmonares/complicações , Neoplasias Primárias Múltiplas/patologia , Adulto , Alquilantes/efeitos adversos , Alquilantes/uso terapêutico , Antineoplásicos/administração & dosagem , Carcinoma de Células Pequenas/induzido quimicamente , Carcinoma de Células Pequenas/patologia , Esquema de Medicação , Quimioterapia Combinada , Feminino , Doença de Hodgkin/patologia , Humanos , Neoplasias Pulmonares/induzido quimicamente , Neoplasias Pulmonares/patologiaRESUMO
A patient with angogenic myeloid metaplasia in blastic transformation with unusually prominent lymphadenopathy simulating lymphoma is described. Interestingly, the initial evidence of myeloblastic transformation was present in the lymph nodes but not in the marrow. Serial bone marrow biopsies showed fibrosis throughout the course of the disease, in spite of a gradual increase in the percentage of peripheral blood myeloblasts. Aneuploidy with a marker chromosome and a Philadelphia-like chromosome was present in the lymph node, bone marrow, and peripheral blood cells. The literature is reviewed regarding the incidence of prominent lymphadenopathy and chromosome abnormalities in agnogenic myeloid metaplasia and other myeloproliferative disorders.
Assuntos
Células da Medula Óssea , Medula Óssea/patologia , Leucócitos/patologia , Mielofibrose Primária/patologia , Aneuploidia , Cromossomos Humanos 19-20 , Cromossomos Humanos 21-22 e Y , Cromossomos Humanos 6-12 e X , Feminino , Humanos , Pessoa de Meia-Idade , Mielofibrose Primária/genéticaRESUMO
Burkitt's lymphoma, associated with massive bone marrow and eyelid involvement that terminated with a manifested leukemic picture, was observed in a Turkish boy. Review of the world literature revealed the fact that Burkitt's lymphoma with a frankly leukemic picture is a rare condition, and usually has a very acute course with a poor prognosis.