Bioinspired gelatin based sticky hydrogel for diverse surfaces in burn wound care.

Proper burn wound management considers patient's compliance and provides an environment to accelerate wound closure. Sticky hydrogels are conducive to wound management. They can act as a preventive infection patch with controlled drug delivery and diverse surface adherence. A hypothesis-driven investigation explores a bioinspired polydopamine property in a gelatin-based hydrogel (GbH) where polyvinyl alcohol and starch function as hydrogel backbone. The GbH displayed promising physical properties with O-H group rich surface. The GbH was sticky onto dry surfaces (glass, plastic and aluminium) and wet surfaces (pork and chicken). The GbH demonstrated mathematical kinetics for a transdermal formulation, and the in vitro and in vivo toxicity of the GbH on test models confirmed the models' healthy growth and biocompatibility. The quercetin-loaded GbH showed 45-50% wound contraction on day 4 for second-degree burn wounds in rat models that were equivalent to the silver sulfadiazine treatment group. The estimates for tensile strength, biochemicals, connective tissue markers and NF-κB were restored on day 21 in the GbH treated healed wounds to imitate the normal level of the skin. The bioinspired GbH promotes efficient wound healing of second-degree burn wounds in rat models, indicating its pre-clinical applicability.

Exploring the styrene metabolism by aerobic bacterial isolates for the effective management of leachates in an aqueous system.

In the present study, the styrene metabolic profile of three aerobic bacterial isolates explored in a batch mode study. The isolates found application in the management of elachates in the waste dump yard. These three bacterial species have different origins and were studied as a single and mixed consortia. The Lysinibacillus strain M01 (from marine sources), Lysinibacillus strain WD03 (from a waste dump yard), and Pseudomonas strain BG07 (from bovine gut) were used in the present study. The styrene concentration was fixed in the range between 0.5 and 1.5 mL L-1. The metabolites obtained upon microbial degradation were assessed using high-performance liquid chromatography (HPLC), UV-visible spectroscopy, and FTIR spectroscopy (Fourier transform infrared spectroscopy). Furthermore, the genes (Sty A, B, C, D, and E) responsible for the degradation of styrene by the three abovementioned isolates were identified using PCR with respective designed primers. Instrumental analyses revealed the presence of phenylacetic acid (PAA) at significant levels in the growth medium after the scheduled experimental period and confirmed the metabolism of styrene by the chosen isolates. Compared to the case of individual cultures, the results of the mixed consortia support the metabolism of styrene at appreciable levels. The present study provides a suitable biological solution for the management of leachates containing styrene and a way to achieve industrially important chemicals (PAA) through a microbially mediated process.

Correction: Handling and managing bleeding wounds using tissue adhesive hydrogel: a comparative assessment on two different hydrogels.

[This corrects the article DOI: 10.1039/C6RA00284F.].