ACC deaminase containing endophytic bacteria ameliorate salt stress in Pisum sativum through reduced oxidative damage and induction of antioxidative defense systems.

Approximately 6% of the world's total land area and 20% of the irrigated land are affected by salt stress. Egypt is one such country affected by salt-stress problems. This paper focuses on the role of isolated bacteria, such as Bacillus subtilis and Pseudomonas fluorescens, in alleviating the harmful effects of salt stress. The results show that the irrigation of plants with different concentrations of saline water (0, 75, and 150 mM NaCl) leads to significantly decreased growth criteria, photosynthetic pigments (i.e., chl a, chl b, and carotenoids), and membrane stability index (MSI) values. Moreover, malondialdehyde (MDA), glutathione content, endogenous proline, the antioxidant defense system, 1-aminocyclopropane-1-carboxylic acid (ACC) content, ACC synthase (ACS), ACC oxidase (ACO), and Na+ content were significantly increased under NaCl-stress exposure. On the contrary, treatment with endophytic bacteria significantly increased the resistance of pea plants to salt stress by increasing the enzymatic antioxidant defenses (i.e., superoxide dismutase, catalase, peroxidase, and glutathione reductase), non-enzymatic antioxidant defenses (i.e., glutathione), osmolyte substances such as proline, and antioxidant enzyme gene expression. As a result, endophytic bacteria's use was significantly higher compared to control values for indole-3-acetic acid (IAA), gibberellic acid GA3, MSI, and photosynthetic pigments. The use of endophytic bacteria significantly decreased Na+ accumulation while, at the same time, promoting K+ uptake. In conclusion, the induction of endophytic bacterium-induced salt tolerance in pea plants depends primarily on the effect of endophytic bacteria on osmoregulation, the antioxidant capacity, and ion uptake adjustment by limiting the uptake of Na+ and, alternatively, increasing the accumulation of K+ in plant tissue.

The natural product biosynthetic potential of Red Sea nudibranch microbiomes.

BACKGROUND: Antibiotic resistance is a growing problem that can be ameliorated by the discovery of novel drug candidates. Bacterial associates are often the source of pharmaceutically active natural products isolated from marine invertebrates, and thus, important targets for drug discovery. While the microbiomes of many marine organisms have been extensively studied, microbial communities from chemically-rich nudibranchs, marine invertebrates that often possess chemical defences, are relatively unknown. METHODS: We applied both culture-dependent and independent approaches to better understand the biochemical potential of microbial communities associated with nudibranchs. Gram-positive microorganisms isolated from nudibranchs collected in the Red Sea were screened for antibacterial and antitumor activity. To assess their biochemical potential, the isolates were screened for the presence of natural product biosynthetic gene clusters, including polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, using PCR. The microbiomes of the nudibranchs were investigated by high-throughput sequencing of 16S rRNA amplicons. RESULTS: In screens against five model microorganisms, 51% of extracts displayed antimicrobial activity against more than one organism, and 19% exhibited antitumor activity against Ehrlich's ascites carcinoma. Sixty-four percent of isolates contained PKS and NRPS genes, suggesting their genomes contain gene clusters for natural product biosynthesis. Thirty-five percent were positive for more than one class of biosynthetic gene. These strains were identified as belonging to the Firmicutes and Actinobacteria phyla via 16S rRNA gene sequencing. In addition, 16S rRNA community amplicon sequencing revealed all bacterial isolates were present in the uncultured host-associated microbiome, although they were a very small percentage of the total community. Taken together, these results indicate that bacteria associated with marine nudibranchs are potentially a rich source of bioactive compounds and natural product biosynthetic genes.

Enhancement of glycerol production by UV-mutagenesis of the marine yeast Wickerhamomyces anomalus HH16: kinetics and optimization of the fermentation process.

The current study aims to enhance glycerol production using UV-mutagenesis of the marine yeast Wickerhamomyces anomalus HH16 isolated from marine sediment collected from South Sinai Governorate, Egypt. Besides optimization of the culture conditions and analyzing the kinetic parameters of growth and glycerol biosynthesis by the mutant strain were studied. The marine yeast isolate HH16 was selected as the front runner glycerol-producer among all tested isolates, with glycerol yield recorded as 66.55 gl-1. The isolate was identified based on the phenotypic and genotypic characteristics of W. anomalus. The genotypic characterization based on the internal transcribed spacer (ITS) sequence was deposited in the GenBank database with the accession number MK182824. UV-mutagenesis of W. anomalus HH16 by its exposure to UV radiation (254 nm, 200 mW cm-2) for 5 min; increased its capability in the glycerol production rate with 16.97% (80.15 g l-1). Based on the kinetic and Monod equations, the maximum specific growth rate (µ max) and maximum specific glycerol production rate (v max) by the mutant strain W. anomalus HH16MU5 were 0.21 h-1 and 0.103 g g-1, respectively. Optimization of the fermentation parameters such as nitrogen source, salinity and pH has been achieved. The maximum glycerol production 86.55 g l-1 has been attained in a fermentation medium composed of 200 g l-1 glucose, 1 g l-1 peptone, 3 g l-1 yeast extract, and 58.44 g l-1 NaCl, this medium was adjusted at pH 8 and incubated for 3 days at 30° C. Moreover, results indicated the ability of this yeast to produce glycerol (73.33 g l-1) using a seawater based medium. These findings suggest the applicability of using the yeast isolate W. anomalus HH16MU5 as a potential producer of glycerol for industrial purposes.