RESUMO
The aim of the present work was to identify the role of functional asymmetry in the cerebral hemispheres in the pathogenesis of infectious and oncological pathology in (CBAxC57BL/6)F1 mice. Mice with lefthemisphere dominance of motor functions ("right-handers") were found to be more resistant to infection with ectromelia virus and to show slower growth of tumors after subcutaneous injection of B-16 melanoma than animals with right-hemisphere dominance of motor control ("left-handers"). Thus, this report provides the first evidence for the role of functional asymmetry of the nervous system in the pathogenesis of ectromelia and melanoma (B-16) in (CBAxC57BL/6)F1 mice.
Assuntos
Ectromelia Infecciosa/fisiopatologia , Lateralidade Funcional , Melanoma Experimental/fisiopatologia , Atividade Motora/fisiologia , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Transplante de NeoplasiasRESUMO
The object of the research was to identify role of functional nervous asymmetry in formation of pathology in experimental animals. The role of functional asymmetry of the brain hemispheres in development of ectromelia and melanoma at (CBAxC57BL/6)F1 mice was investigated. It has been established, that mice with domination of motor functions in the left hemisphere ("right-handed") are more resistant against infection with ectromelia virus, and melanoma B-16 grows more slowly in these mice as compated with animals with domination of motor functions of the right hemisphere ("left-handed"). Hence, for the first time the role of functional nervous asymmetry in pathogenesis of ectromelia and melanoma (B-16) in mice (CBAxC57BL/6)F1 is established.
Assuntos
Ectromelia Infecciosa/fisiopatologia , Lateralidade Funcional , Melanoma Experimental/fisiopatologia , Atividade Motora/fisiologia , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Transplante de NeoplasiasRESUMO
We studied the effect of transplantation of splenic lymphoid cells on functional activity of the nervous (orientation and exploratory behavior and expression of genes for interleukin-1beta, type 1 interleukin-1 receptor, and erythropoietin in the brain) and immune system (cellular and humoral immune response, proliferative activity of immunocompetent cells, and expression of cytokine genes in splenocytes) in syngeneic animals with different behavioral characteristics. Intravenous injection of the lymphoid fraction of splenocytes from donor mice with a certain behavioral pattern in the open-field test had a modulatory effect on vertical locomotor activity of syngeneic recipient mice. The increase or decrease in vertical locomotor activity due to transplantation of immunocompetent cells was accompanied by specific changes in mRNA level for erythropoietin receptor and type 1 interleukin-1 receptor in brain cells of recipient mice. The regulation of orientation and exploratory behavior was also accompanied by changes in functional activity of the immune system in recipient animals. It was manifested in modulation of proliferative activity of thymic and splenic cells and cytokine gene expression in splenocytes.
Assuntos
Encéfalo/fisiologia , Sistema Imunitário/fisiologia , Transfusão de Linfócitos , Linfócitos/citologia , Baço/citologia , Animais , Proliferação de Células , Comportamento Exploratório/fisiologia , Interleucina-1beta/metabolismo , Locomoção/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , RNA Mensageiro/metabolismo , Receptores da Eritropoetina/metabolismo , Receptores Tipo I de Interleucina-1/metabolismoRESUMO
The given review is devoted to studying of a role cytokine in immune, nervous and endocrine systems. Ways of cytokine action in a brain are described. Are discussed neurotrophic and behavioural effects cytokine in a brain and mechanisms of their action. Acentuated, that there are groups cytokine which mainly show activity, operating in immune system, other groups cytokine are most active in nervous system, carrying out neurotrophic, neuroprotection functions or expressed and are produced in endocrine system and in a greater degree function as hormones. New approaches to functional classification cytokine are discussed.
Assuntos
Citocinas/fisiologia , Sistemas Neurossecretores/imunologia , Sistemas Neurossecretores/fisiologia , Animais , Comportamento/fisiologia , Encéfalo/imunologia , Encéfalo/fisiologia , HumanosAssuntos
Comportamento do Adolescente/fisiologia , Sistema Nervoso Autônomo/imunologia , Comportamento Infantil/fisiologia , Emoções/fisiologia , Sistema Imunitário/fisiologia , Motivação , Adolescente , Antígenos CD/sangue , Criança , Feminino , Antígenos HLA-DR/sangue , Humanos , Leucócitos Mononucleares/imunologia , Masculino , Linfócitos T/imunologiaRESUMO
Immunological indices were assayed in 167 patients with gastric cancer. Tumor growth involved reduction in levels of neutrophil peroxide (p<0.05), CD3+ and CD19+-cells (p<0.05) as well as intensification of apoptosis of lymphocytes (p<0.05). As monocytic and granulocytic phagocytosis (p<0.001) and HLA-DR-expression in monocytes (p<0.001) diminished, and mitogen-induced proliferative activity of mononuclear cells suppressed (p<0.05), total and relative levels of CD8+-cells (p<0.05) and spontaneous proliferative activity of mononuclear cells (p<0.05) in peripheral blood increased. Such changes should not be reganded as expression of immunosuppression alone. Mechanisms of immunological failure inherent in tumor growth need to be investigated further.
Assuntos
Neoplasias Gástricas/imunologia , Adulto , Idoso , Apoptose , Feminino , Antígenos HLA-DR/imunologia , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , FagocitoseRESUMO
Intravenous injection of adherent splenocyte fraction from donor (CBAxC57Bl/6)F(1) mice characterized by specific open-field behavior modified this behavior in syngeneic recipient mice. This was paralleled by appropriate changes in the levels of IL-1b and type 1 IL-1 receptor mRNA in the brain cells of recipient mice. Hence, we demonstrated the possibility of directed regulation of orientation and exploratory behavior in mice by transplantation of immunocompetent cells. Mononuclear phagocytes play an important role in this phenomenon.
Assuntos
Encéfalo/metabolismo , Transplante de Células/métodos , Citocinas/metabolismo , Comportamento Exploratório , Orientação , Fagócitos/transplante , Animais , Cruzamentos Genéticos , Citocinas/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Baço/citologia , Baço/imunologia , Transplante IsogênicoRESUMO
Over the last years the data on the changes of immunological parameters in traumatic and pathological lesions of brain hemispheres has been obtained by neuroimmunology, a science investigating mechanisms, principles, regularities as well as interaction and integration of immune and nervous systems. However many aspects of the field remain unclear and need further study. In this context parameters of immune system in patients with acute stroke have been examined. These parameters proved to correlate with the disease severity suggesting that the main parameters of cell and humoral patterns of immune system depend on the structural and functional state of brain hemispheres.
Assuntos
Sistema Imunitário/imunologia , Acidente Vascular Cerebral/imunologia , Idoso , Antígenos CD/sangue , Antígenos CD/imunologia , Biomarcadores/sangue , Feminino , Citometria de Fluxo , Antígenos HLA-DR/sangue , Antígenos HLA-DR/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Acidente Vascular Cerebral/sangue , Linfócitos T/imunologiaRESUMO
A link between HLA allelic variants and long-term results of surgery for gastric tumors was established on the basis of a 10-years follow-up of 112 cancer patients (stage I-II--37.9, III-IV--62.1%; radical surgery--44.6%). HLA class I was studied in a lymphocytotoxic test; HLA class II--gene DRBI specificity using polymerase chain reaction of peripheral blood cell DNA. The control group included healthy subjects living in the city of Novosibirsk (n = 341). High frequency of antigens HLA-B41, -DR1, -DR7 (p < 0.01) co-occurred with HLA-A2, -B12, -B13 and -B18 presence (p < 0.05) in breast cancer patients. Clinical manifestations of cancer were shown to develop in HLA-A1, -B8, -B15, -DR3 and -DR5 carriers at early stages. Tumor development at later stages (III-IV) was associated with HLA-A2, -B12, -B17, -B35, -B41 and -DR7. A link was registered between lethality rate, on the one hand, and HLA-A3, -B22, and, in particular, DR4, on the other, while remission of more than 7-years--with HLA-A11, -B13, -B21 and -DR5. HLA-B22/DR3 phenotype involved worse prognosis in radically-treated patients whereas that of HLA-B8/DR3--a better one.
Assuntos
Genes MHC da Classe II , Genes MHC Classe I , Antígenos HLA/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/imunologia , Idoso , Testes Imunológicos de Citotoxicidade , Feminino , Frequência do Gene , Humanos , Masculino , Pessoa de Meia-Idade , Fenótipo , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Prognóstico , Neoplasias Gástricas/mortalidade , Neoplasias Gástricas/cirurgia , Fatores de TempoRESUMO
We revealed some relationships between the level of orientation and exploratory behavior, functional activity of the immune system, and structural and functional organization of the CNS in animals. Significant differences in brain morphology and expression of cytokine IL-1 beta, type I IL-1 receptor, and erythropoietin receptor genes in brain cells were detected in (CBA x C57Bl/6)F1 mice with different initial levels of orientation and exploratory behavior. Immunocompetent cells of mice with high and low levels of exploratory behavior differ by spontaneous and mitogen-induced proliferative activity. The formation of humoral and cellular immune response in these animals causes opposite changes in exploratory behavior and the type of these changes depends on the initial level of this behavioral reaction.
Assuntos
Comportamento Exploratório/fisiologia , Neuroimunomodulação/fisiologia , Orientação/fisiologia , Animais , Encéfalo/anatomia & histologia , Encéfalo/imunologia , Encéfalo/fisiologia , Expressão Gênica , Técnicas In Vitro , Interleucina-1/genética , Ativação Linfocitária , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Receptores da Eritropoetina/genética , Receptores de Interleucina-1/genética , Receptores Tipo I de Interleucina-1RESUMO
Tissue-specific immune reactions were used to develop a method of dealing with malignant tumors. Injection of proteins isolated from lung tissue of mice (CBAxC57BL/6)F1, of varying age, into mice Balb/c with urethane-induced lung tumors cut the number of such tumors by half. Direct correlation was found between the magnitude and nature of effect and level of lung protein injection.
Assuntos
Neoplasias Pulmonares/imunologia , Neoplasias Pulmonares/terapia , Proteínas/administração & dosagem , Proteínas/imunologia , Animais , Carcinógenos , Neoplasias Pulmonares/induzido quimicamente , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/terapia , UretanaRESUMO
The intensity of delayed type hypersensitivity reactions in the left and right paws was studied in mice divided into left- and right-pawed by the motor asymmetry of the brain. The reaction was more pronounced in the left paw in all animals irrespective of motor asymmetry. Motor asymmetry of the brain hemispheres little contributed to the manifestation of differences in the delayed type hypersensitivity reactions in the left and right paws. The authors concluded that asymmetry of cellular immunity is determined by functional asymmetry of cells in regional lymph nodes.
Assuntos
Lateralidade Funcional/fisiologia , Hipersensibilidade Tardia/fisiopatologia , Animais , Masculino , CamundongosRESUMO
Lower natural killer cells activity (NKCA), varying stage of anemia and in vitro depressed colony-forming ability of committed precursors of hemopoiesis were identified in 50 gastric cancer patients. The patients with different patterns of the vegetative nervous system had tumors with a range of adenocarcinoma cell differentiation. A relationship was established between anemia stage, colony-forming ability of committed precursors of hemopoiesis, on the one hand, and vegetative nervous system pattern, stage of tumor cell differentiation and, to a less extent, NKCA, on the other.
Assuntos
Adenocarcinoma/diagnóstico , Adenocarcinoma/imunologia , Anemia/etiologia , Células Matadoras Naturais/imunologia , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/imunologia , Adenocarcinoma/complicações , Adulto , Anemia/imunologia , Feminino , Hematopoese , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Neoplasias Gástricas/complicações , Ensaio Tumoral de Célula-TroncoRESUMO
We demonstrated opposite changes in the orientation and exploratory behavior of (CBAxC57Bl/6)F(1) mice in the open field test during the formation of primary humoral immune response. These changes depended on initial behavioral activity: the exploratory behavior was suppressed in animals with initially high activity, stimulated in animals with medium reaction, and remained unchanged in mice with initially low activity. The detected changes in the exploratory behavior during the formation of immune response were leveled in the total population (not divided by initial behavioral status).
Assuntos
Comportamento Animal , Sistema Imunitário , Animais , Citocinas/metabolismo , Eritrócitos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Receptores de Interleucina-1/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , OvinosRESUMO
The role of functional asymmetry of the brain and bone marrow in the formation of exogenous 8-day splenic CFU was studied in (CBAxC57Bl/6)F(1) mice. The intensity of hemopoiesis depended on the motor asymmetry of donors and recipients of bone marrow cells and differed for bone marrow transplantations from the right and left femoral bones.
Assuntos
Células da Medula Óssea/citologia , Células da Medula Óssea/fisiologia , Encéfalo/anormalidades , Encéfalo/patologia , Hematopoese , Células-Tronco Hematopoéticas/citologia , Animais , Divisão Celular , Fêmur/metabolismo , Lateralidade Funcional/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Baço/metabolismo , Fatores de TempoRESUMO
Interleukin-1beta mRNA, interleukin-1beta receptor mRNA, and erythropoietin receptor mRNA are expressed in the brain of (CBAxC57Bl)F1 mice. Immunization with sheep erythrocytes stimulated the expression of these cytokines in the cerebral hemispheres. Injection of recombinant erythropoietin reduced expression of interleukin-1beta, interleukin-1beta receptor, and erythropoietin receptor genes in the brain of experimental animals and considerably increased their motor activity in the open field test.
Assuntos
Comportamento Animal , Encéfalo/metabolismo , Citocinas/metabolismo , Animais , Citocinas/genética , Lateralidade Funcional , Interleucina-1/genética , Interleucina-1/metabolismo , Interleucina-1beta , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo , RNA Mensageiro/metabolismo , Receptores da Eritropoetina/genética , Receptores da Eritropoetina/metabolismo , Receptores de Interleucina/genética , Receptores de Interleucina/metabolismoRESUMO
The present investigation develops a previously published concept on integration of the immune, endocrine, and nervous systems in a universal structural and functional block (IMEN system). Splenocytes from donor (CBAxC57Bl/6)F1 mice characterized by specific behavior in the open field test modified the behavior of syngeneic recipient mice after intravenous infusion.
Assuntos
Transplante de Células , Baço/imunologia , Animais , Comportamento Animal , Cruzamentos Genéticos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Baço/citologia , Fatores de TempoRESUMO
Now there is a growing evidence that erythropoietin receptors (Epo-R) are present also in some nonhematopoietic tissues such as endothelial cells and fetal cells of neural origin, although the physiological role of Epo-R at these sites is unclear. There are some speculations that Epo-R may be expressed on cells only in the developing CNS. The objective of this study was to determine whether Epo-R mRNA may be expressed in the brain hemispheres of Balb/c mice of different age groups: 1) newborn mice, 2) young 2 months old mice, 3) old 1.8 year old mice. We also studied the in vivo effect of recombinant erythropoietin on the expression of Epo-R mRNA in the brain hemispheres of (CBA x C57BL)F1 mice by RT-PCR. We have detected the existence of Epo-R mRNA expression in brain hemispheres of all the groups, but in old mice this expression was significantly higher. We have discovered a decrease in Epo-R mRNA expression in brain hemispheres of (CBA x C57BL)F1 mice 24 h after in vivo administration of recombinant erythropoietin. The Epo-R mRNA expression in the left brain hemispheres of (CBA x C57BL)F1 was considerably higher than in the right one.
Assuntos
Encéfalo/metabolismo , RNA Mensageiro/biossíntese , Receptores da Eritropoetina/biossíntese , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Encéfalo/citologia , Contagem de Células , Masculino , CamundongosRESUMO
We studied the role of functional asymmetry in mouse brain and thymus in the realization of humoral immune response. We concluded that not only nervous system asymmetry, but also immune system asymmetry and the relationship between cerebral hemispheres and cells of the right and left thymus lobes play an important role in the regulation of immune response.
Assuntos
Formação de Anticorpos , Córtex Cerebral/fisiologia , Lateralidade Funcional , Timo/fisiologia , Animais , Eritrócitos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Atividade Motora , Ovinos , Linfócitos T/transplante , Timo/citologiaRESUMO
We revealed a modulating effect of cell immune response on exploratory activity of (CBA degrees C57Bl/6)F1 mice depending on their initial behavioral profile. The formation of BCG-induced delayed-type hypersensitivity was accompanied by stimulation of the exploratory behavior in animals with initially low or intermediate activity, but did not change this parameter in behaviorally active mice.