RESUMO
Sequence-based typing (SBT) is a discriminatory method widely used to genotype Legionella pneumophila strains. A total of 86 clinical L. pneumophila serogroup 1 (sg1) isolates, collected between January 2000 and December 2010 in the two Belgian National Reference Centres for Legionella pneumophila, were genotyped using the internationally standardised SBT protocol of the European Working Group for Legionella Infections (EWGLI). The isolates could be classified into 31 different sequence types (ST, index of diversity: 0.879). The obtained STs were submitted to the EWGLI SBT-database for L. pneumophila. In our study, ST47 (27.9%) and ST1 (19.8%) were the most frequently detected STs. The detected profiles were a combination of both frequently isolated and unique STs, and of both worldwide distributed and more local strains. Two STs, ST880 and ST881, were new to the EWGLI database. In conclusion, we characterised L. pneumophila sg1 isolates with the SBT method, and created a Belgian profile database that will be useful for future epidemiological studies.
Assuntos
Legionella pneumophila/isolamento & purificação , Doença dos Legionários/diagnóstico , Análise de Sequência de DNA/tendências , Sorotipagem/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Bélgica/epidemiologia , Monitoramento Ambiental , Feminino , Marcadores Genéticos , Sistemas de Informação Geográfica , Humanos , Legionella pneumophila/classificação , Doença dos Legionários/epidemiologia , Masculino , Pessoa de Meia-Idade , Técnicas de Diagnóstico Molecular/métodos , Análise de Sequência de DNA/estatística & dados numéricosRESUMO
A case of purple urine bag syndrome, a rare condition in which the urinary catheter bag of chronically catheterised patients develops a discolouration, is reported. The excretion of indoxyl sulphate, an intermediate in the causal mechanism of this unusual phenomenon, was measured using Ehrlich's reagent and found not to be elevated in this 77 year-old man, when compared to elderly male control subjects.