RESUMO
This report describes the clinical presentation, pathology and molecular diagnosis of canine parvovirus infection in male Boerboel and female Alsatian puppies. The history of the dogs was considered, examined clinically for vital parameters, haemogram changes and faeces screened for parasites and canine parvovirus faecal antigen. Tissue samples were taken at necropsy for confirmatory diagnosis using histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. There was a severe regenerative anaemia, leucopenia and lymphopaenia. The positive antigen faecal test and pathological findings of haemorrhagic enteritis suggested canine parvoviral enteritis disease. Polymerase chain reaction and sequence analysis confirmed canine parvovirus-2a as the aetiology of the disease. Informed management is important to avoid complications resulting from secondary to severe dehydration, hypovolemia from marked gastrointestinal fluid and protein loss and sepsis from bacterial translocation and leukopenia.
