[The changing of cell-free DNA properties of peripheral blood and TCR-mutant cell frequency in individuals exposed to ionizing radiation].

Some properties of the cell-free DNA (cfDNA) of peripheral blood plasma were assessed in 153 employees of atomic industry enterprises. The contents of ribosomal repeat (rDNA) and its concentration in plasma increased in cfDNA of the group of persons in comparison with non-irradiated individuals. The contents of satellite III in cfDNA of donors and of irradiated persons do not differ and less than in DNA nucleus. The correlation between cumulative dose of radiation, contents of rDNA in cfDNA and the frequency of lymphocytes bearing mutations at T-cell receptor (TCR) locus was obtained. The definition of three indications in irradiated persons: the contents of ribosomal genes in cfDNA, TCR-mutant cell frequency and concentration of ribosomal genes in blood plasma--may be useful for revealing individuals in organism of which an intensive cell apoptosis takes place and there is an increased probability of carcinogenesis and of progress of disease of immune system.

[Ribosomal genes in inbred mouse strains: interstrain and intrastrain variations of copy number and extent of methylation].

Quantitative dot hybridization was used to estimate the rDNA copy number in brain tissues of five inbred mouse strains (AKR/JY, NZB/B1OrlY, CBA/CaLacY, 101/HY, and 129/JY), which were obtained from the collection of the Research Center of Biomedical Technologies (Y). In each strain, 9-12 mice aged 1-2 months were examined. The rDNA copy number per diploid genome in strains AKR (range 105-181, mean +/- SD 136 +/- 27) and NZB (129-169, 148 +/- 12) was significantly lower than in strains CBA (172-267, 209 +/- 31), 101 (179-270, 217 +/- 30), and 129 (215-310, 264 +/- 33). Mice of strain NZB were relatively homogeneous in this trait (CV = 8.1%). Strains AKR, CBA, 101, and 129 displayed significant between-group differences, CV varying from 12.5 to 19.9%. The same DNA specimens were digested with MspI or HpaII and used to estimate the extent of methylation of the 28S rDNA region. Regardless of the strain, all mice could be classed into two groups. One group (20 mice) had a methylated fraction accounting for less than 8% of rDNA and included all nine mice of strain NZB, seven out of nine mice of strain 101, and three out of ten mice of strain 129. In the other group (29 mice), the methylated fraction varied from 18 to 38%. A possible role of methylation and the genome dosage of ribosomal genes in phenotypic variation (quantitative trait variation) of inbred mouse strains is discussed.

[The use of discrete characters in discriminant analysis for diagnosis of pulmonary tuberculosis and for classification of patients differing in treatment efficiency based on polymorphisms at nine codominant loci-HP, GC, TF, PI, PGM1, GLO1, C3, ACP1 and ESD]. / Ispol'zovanie diskretnykh priznakov v diskriminantnom analize pri diagnostike tuberkuleza legkikh i pri klassifikatsii bol'nykh s razlichnoi éffektivnost'iu lecheniia po raspredeleniiu polimorfizmov v deviati kodominantnykh lokusakh-HP, GC, TF, PI, PGM1, GLO1, C3, ACP1 i ESD.

Discriminant analysis was used to differentiate patients with pulmonary tuberculosis (N = 106) from healthy individuals (N = 328) and patients whose treatment was efficient (N = 71) from those whose treatment was inefficient (N = 35). The analysis involved the data on nine polymorphic codominant loci: HP, GC, TF, PI, PGM1, GLO1, C3, ACP1, and ESD. The loci were selected by significance of differences in genotype frequencies between tuberculosis patients and healthy controls (GC, TF, PI, C3, ACP1) or between the two groups of patients differing in treatment efficiency (HP, GC, PI, PGM1, C3, ESD). Discrimination was based on a graphic method of Bayes classification procedure with a single-variate nomograph allowing easy estimation of the a posteriori probabilities for an individual to be classified. The two groups of patients proved to be discriminated sufficiently well (probability of misclassification Perr = 0.24), whereas discrimination between tuberculosis patients and healthy individuals was less efficient (Perr = 0.33). The method was proposed as a means of predicting the efficiency of treatment in pulmonary tuberculosis. Along with clinical, roentgenological, and laboratory examination, discriminant analysis may be employed as an accessory test in diagnostics of pulmonary tuberculosis, especially when the diagnosis is questionable.

[Heterozygosity in patients with pulmonary tuberculosis showing varying responses to therapy]. / Issledovanie urovnei geterozigotnosti u bol'nykh tuberkulezom legkikh s razlichnoi éffektivnost'iu lecheniia.

The distribution of the levels of heterozygosity was analyzed by 9 loci of genetic markers: PI, TF, PGM1, ACPI, HP, GC, GLO1 C3, and ESD in two groups of patients with pulmonary tuberculosis who had improvements (Group 1, n = 71) and failures (Group 2, n = 35). The heterozygosity observed in the groups was compared with that calculated by the Hardy-Weinberg law by using data on healthy controls (n = 328; the locus ESD was investigated in 78 healthy individuals). The analysis indicated that there were statistically significant deviations of the observed heterozygosities, g1, at 4 loci (GC, PI, C3, and ACPI) from the expected ones; h1 calculated from the data in the control group. The observed heterozygisities were higher than the expected ones at 3 loci (PI, C3, and ACPI), and at the GC locus. the observed heterozygosity being lower than the expected one. Comparing the observed heterozygosities. g1, within the loci, by using Fisher's exact test revealed significant differences between the groups of patients and healthy controls at the same loci, which showed significant differences between the observed and expected heterozygosities. There were no differences between the groups of patients by the observed heterozygosities. The mean expected heterozygosity were h = 0.386 +/- 0.056. The mean observed heterozygosity, were g = 0.415 +/- 0.037, 0.402 +/- 0.061, 0.371 +/- 0.055 in Groups 1 and 2 and in the controls, respectively. There were no differences between the mean expected and obsorved heterozygosities or between the mean observed heterozygosities in the three groups under study. It is proposed that a single locus rather than the mean heterozygosities should be used as a generalized nonspecific measure of genetic control over diseases while the former can show the involvement of a specific marker locus to develop a disease, the latter can simply veil the effects of each of the loci alone. Thus, the findings produce strong evidence for that there is a genetic control in the development of pulmonary tuberculosis.

[Analysis of heterozygosity levels at P1,TF, PGM1, ACP1, HP, GC, GLO, C3, and ESD loci in pulmonary tuberculosis patients with different treatment outcomes]. / Analiz urovnei geterozigotnosti po lokusam PI, TF, PGM1, ACP1, HP, GC, GLO1, C3 i ESD u bol'nykh tuberkulezom legkikh s razlichnoi éffektivnost'iu lecheniia.

Heterozygosity at nine genetic loci (PI, TF, PGM1, ACP1, HP, GC, GLO1, C3, and ESD) was analyzed in pulmonary tuberculosis patients with good (group 1, N = 71) and poor (group 2, N = 35) response to treatment. The observed heterozygosities were compared with the expected values, which were calculated from allele frequencies in a control sample of healthy individuals (N = 328 with all but one locus and 78 with ESD) according to Hardy-Weinberg expectations. The analysis showed that the observed heterozygosities gl of patients significantly differed from the expected values hl in the case of four loci (GC, PI, C3, and ACP1). The observed heterozygosity was higher than expected in three cases (PI, C3, and ACP1) and lower then expected (GC) in one case. When data on each individual locus were compared using Fisher's exact test, both groups of patients proved to significantly differ (PF < 0.05) from the control group in the same four loci. No difference in observed heterozygosity was detected between the two groups of patients. The mean expected heterozygosity was h = 0.386 +/- 0.00674; the mean observed heterozygosity was g = 0.415 +/- 0.02 in group 1, g = 0.402 +/- 0.026 in group 2, and g = 0.371 +/- 0.00955 in the control group. The t test did not reveal a significant difference between the mean values of expected observed heterozygosities. Heterozygosity at individual loci, rather than mean heterozygosity, was proposed as an integral nonspecific indicator of the genetic control of a disease, because the former directly implicates individual marker loci in the development of a disorder, whereas effects of individual loci may eliminate each other when mean heterozygosity is computed. Based on the results obtained, a genetic control was assumed for the development of the tuberculosis process in the lungs.

Apolipoprotein B 3'-VNTR polymorphism in the Udmurt population.

An analysis of a highly polymorphic region of the apolipoprotein B gene 3'-end DNA (Apo B 3'-VNTR), represented by 10 alleles, was carried out using the polymerase chain reaction. Data inferred from the principal component analysis indicate that the Udmurts occupy an isolated position among the populations constituting the northern branch of Caucasoid peoples.

[Blood protein phenotypes and effectiveness of the treatment of patients with pulmonary tuberculosis]. / Fenotipy belkov krovi i éffektivnost' lecheniia bol'nykh tuberkulezom legkikh.

Genetic polymorphism at 9 independent loci (HP, GC, TF, PI, PGM1, GLO1, C3, ACP1, and ESD) was studied in two groups of patients with pulmonary tuberculosis and healthy controls. The patients were subdivided into two subgroups depending on their response to chemotherapy: 1) responsive and 2) unresponsive to adequate chemotherapy. The control (Group 3) comprised 327-329 healthy persons; only 78 of them were phenotyped for ESD-locus. A pairwise comparison of arcsinus-transformed both phenotypic and allelic frequencies using t-test revealed significant differences between Groups 1 and 3 in phenotypes--GC 1F-1S, (P = 3.73 x 10(-4), C3 F-S (P = 4.10 x 10(-5), C3 S-S (P = 1.9 x 10(-6) and in alleles--C3*F (P = 4.2 x 10(-6), C3*S (P = 1.7 x 10(-6). These differences are reliable at the levels of significance, Pc corrected by all numbers of times, k, of independent pairwise comparisons (k = 126 for phenotypes, Pc = 0.00041; k = 60 for alleles, Pc = 0.000855). Differences between Groups 2 and 3 were significant as well: there were 8 P values both for phenotypes and alleles, which rejected the null-hypothesis at 5% significance while the expected numbers of times, kch, to reject the null-hypothesis by chance, were kch = 6.3 for phenotypes and kch = 3 for alleles. Differences between the two subgroups of patients were found to be insignificant because the P values were obtained, that rejected the null-hypothesis only 6 and 3 times for phenotypes and alleles, respectively. Principal component analysis showed that the GLO1 locus was not informative for the differences in the groups studied. The perspectives of further analysis of the data presented using the remaining 8 loci are discussed.

[Discriminant analysis of clinical laboratory data for diagnosis and treatment of tuberculosis and lung cancer]. / Diskriminantnyi analiz laboratornykh pokazatelei pri diagnostike i lechenii tuberkuleza i raka legkogo.

The study is based on the clinical observation and examination of 33 patients with stages 1 or 2 lung cancer (Group 1), 53 with stage 3 lung cancer (Group 2), and 44 patients with pulmonary tuberculosis (Group 3). All the patients underwent surgical treatment. A control group comprised 50 apparently healthy individuals. The clinical laboratory studies included general peripheral blood analysis of ESR, the levels of hemoglobin, leukocytes, lymphocytes, total protein and albumin. ANOVA revealed a highly significant (p < 0.00004) differences in 5 of the 6 study laboratory parameters, variations in the level of total protein between the groups did not differ from that in the groups (p = 0.25). ANOVA revealed significant covariation of the levels of protein with ESR (p < 0.00004) and those of albumin (p < 0.00004). When variations in the latter were eliminated, the effect of the groups on total protein variation was highly significant (p = 0.0018). Discriminant analysis showed that the parameters studied were of diagnostic value in the differential diagnosis: probabilities of correct subdivision of patients ranged 72.6 to 78%, those of erroneous classification being 27.4 and 22%, respectively. The actual probabilities of misclassification were twofold lower. The problems in the employment of the discriminant procedure in the differential diagnosis of the study diseases using the specific contingent principle are discussed and a specific clinical observation is cited as an example.

[Genetic aspects of endometriosis: features of the distribution of polymorphic gene frequencies]. / Geneticheskie aspekty éndometrioza: osobennosti raspredeleniia chastot polimorfnykh genov.

In a group of patients with endometriosis and in a control group of healthy women, the polymorphism of the following systems were studied: ABO and RH blood-group systems; serum proteins haptoglobin (HP), transferrin (TF), vitamin D-transporting protein (GC), protease inhibitor (PI), and the third component of the complement (C3); serum enzymes-amylase of the loci 1 and 2 (AMY1 and AMY2), pseudocholinesterase (E2), and alkaline phosphatase (PP); erythrocytic enzymes-acid phosphatase (ACP1), phosphoglucomutase (PGM1), superoxide dismutase (SOD-A), esterase D (ESD), and glyoxalase (GLO1). Statistically significant differences between the groups compared were established for five genetic systems: ABO, E2, C3, TF, and PGM1. Among patient with endometriosis, the rare alleles of the locus ESD-ESD5 and ESD7-were found, along with ESD 5-5 homozygotes. Several genetic loci can be involved in the pathogenesis of endometriosis; their products can be specifically realized due to peculiarities of biochemical reactions in the organisms of people predisposed to this pathology.

[Possible connection between the level of heterozygosity of biochemical gene markers in patients with lung diseases]. / Vozmozhnaia sviaz' mezhdu urovnem geterozigotnosti po biokhimicheskim markeram genov u bol'nykh s legochnoi patologiei.

The comparative heterozygosity level was estimated in patients suffering from squamous epithelial lung cancer (SELC) and in patients with chronic pneumonia with bronchiectases. To estimate heterozygosity, seven loci, reflecting normal diversity in human populations, were used (HP, TF, GC, PI, GL01, ACP1, PGM1). SELC patients with an uncomplicated postoperative period were distinguished by an increase in the level of observed heterozygosity (Hzero = 0.3916) in comparison with the theoretically expected value (H(e) = 0.4361). Patients having chronic pneumonia with bronchiectases with a complicated postoperative period were distinguished by an increase in the observed and expected level of heterozygosity (Hzero = 0.3737, H(e) = 0.3837) in comparison with that in the patient cohort with an uncomplicated postoperative period. The Wright's fixation index had a high value in the SELC cohort with an uncomplicated postoperative period (F = 0.1073) and a low value in patients with complicated cases (0.0048), witnessing the polar divergence of those patients from the total group of patients (0.0598) and the control (0.0388). The D criterion, reflecting deviation from the maximum heterozygosity level, distinguished the SELC patient cohort with a complicated postoperative period from patients with uncomplicated cases by the HP, GC, and ACP1 loci. The D criterion distinguished the SELC patients from the healthy control groups by the PGM1 locus.

[Effect of hereditary factors on tolerance for surgical treatment in patients with lung cancer]. / Vliianie nasledstvennykh faktorov na perenosimost' khirurgicheskikh operatsii u bol'nykh rakom legkogo.

Genetic polymorphism at 10 independent loci (ABO, RH, HP, GC, PI, TF, ACP1, PGM1, GLO1, and PTC) was studied in male patients with lung squamous cell carcinoma. These patients were divided into two groups, depending on their tolerance for surgical intervention and on the postoperative course: (1) patients with an uneventful postoperative period and (2) patients with postoperative complications. The genetic structure of the combined sample at the loci studied did not differ from that of the control group consisting of health people (population control). Genotypic differences might manifest at the postoperative stage rather than at the onset of the disease, and determine the presence of postoperative complications. However, comparative analysis of the two groups of patients revealed their polar divergence in respect to phenotype and gene frequencies at certain loci. Moreover, the genotypic structure of patients in both groups differed from that in the combined sample and in the population control. In the group with postoperative complications, higher frequencies of the alleles GC*1F, ACP1*A, and HP*2 were observed. By contrast, the group of patients with an uneventful postoperative period demonstrated prevalence of the alternative alleles of these loci: GC*2, ACP1*B, and HP*1. The greatest difference in the distribution of informative allele frequencies was observed between the group of patients with postoperative complications and the control group. This is evidence that these groups significantly differ in their genetic structure. Such divergence is largely determined by the polymorphic multifunctional systems of serum proteins.

[Role pf genetic and other biomarkers in the prognostication of postoperative course in patients with lung cancer]. / Rol' geneticheskikh i drugikh biomarkerov v prognozirovanii techeniia posleoperatsionnogo perioda u bol'nykh rakom legkogo.

Relationships between genetic polymorphisms (ABO, RH, HP, TF, GC, Pi, ACP1, PGM1, GLO1, PTC) and some clinical, biochemical, and functional parameters were studied in patients with epidermoid carcinoma of the lung who were divided into 2 groups: those with uncomplicated and complicated postoperative courses of the disease. They were found to be different in the two groups. The values of ESR, albumin, lymphocytes, vital capacity, and RQ are the most distinctive signs that differentiate the patient groups. A high correlation was found between the signs in patients with an uncomplicated postoperative course. A less correlation between the signs, as a higher intergroup variability in the majority of the signs under study suggests that there is a significantly impaired physiological homeostasis in the group of patients with a complicated course. Comparing the mean values and dispersions shows their equal direction in the two groups of patients irrespective of their genetic polymorphism. The GC system is associated with profound changes of the studied signs in the group of patients with an uncomplicated course and GC*1F carriage should be regarded as a poor factor in the prognosis of the disease.

[Clinico-genetic aspects of cutaneous melanoma. II. Interconnection and pathogenetic commonality with dysplastic nevus syndrome]. / Kliniko-geneticheskie aspekty melanomy kozhi. II. Vzaimosviaz' i patogeneticheskaia obshchnost' melanomy kozhi s displasticheskimi nevusami.

Evidence for the role of dysplastic nevi (DN) in the development of cutaneous malignant melanoma (CMM) is presented. Primary multiple foci of CMM were found considerably more frequently in individuals with DN. The frequency of primary multiple CMM was found to be 3.1% in males with DN and 0.9% in males without DN; in females, 6.8 and 0.6%, respectively. Genetic correlation analysis was performed to determine the genetic interrelation between DN and CMM. In general, the genetic correlation coefficient was 0.9; i.e., predisposition to DN and CMM is determined by common genes for 90%. The frequency and distribution of constitutive fragile sites in chromosomes of peripheral lymphocytes was studied by the method of principal components for discrete variables. The site 1p22 is responsible for variability of the traits CMM and DN for 98.5%. On the one hand, this suggests that one of the supposed genes for CMM can be located at 1p22; on the other hand, CMM and DN are likely to have a common genetic determination or to be very tightly linked. Estimates of risk for the development of CMM in patients' relatives are given with reference to the variants of CMM manifestation and presence of DN.

[Effects of maximal possible potential selection in the world population. New data on selection structure in the CIS nations]. / Osobennosti deistviia maksimal'no vozmozhnogo potentsial'nogo otbora v mirovom narodonaselenii. Novye dannye o strukture otbora v SNG.

New information on maximal possible potential selection and its component values in some ethno-territorial groups in CIS was presented. The heterogeneity observed in the Crow's index and its components can be explained as a result of the differences in the social economic status of the groups studied and the influence of climate geographical factors. The data gathered during the biodemographical study of 67 populations allowed to detect regularities of the effects of selective factors in world population: non-random and discrete nature of considered populations distribution in the coordinate space of selection components associated with differential mortality (I) and differential fertility (I) was shown. Differentiation of three big aggregations of populations was shown: urbanized contemporary communities with low I values; small endogamous populations, mostly of hunters and gatherers; small towns' populations and rural populations with balanced reproductive indices. Microevolutionary changes take place in the latter conglomerate even now, statistically subdividing it into two clusters. A proposition was made about the existence of "ecological optimum" for populations intermediate between advanced industrial communities and communities of hunters and gatherers, corresponding to the population size and the nature and rate of population reproduction.

[Feasibility of establishing genetic homeostasis in human populations using a complex of genetic markers]. / Vozmozhnost' ustanovleniia geneticheskogo gomeostaza v populiatsiiakh cheloveka po kompleksu markerov genov.

The information that we have about functional connection of different phenotypes of independent loci served for identification of their reciprocal behavior in cohorts differed in healthy status from the same population. Coefficients of correlation were computed between phenotypes according to the scheme: everyone with all in a Buryat, on the basis of the distribution of 17 genetic loci. Their space was transformed to a standardized one of eigenvectors. Calculation of the distances between genotypes was performed using the Euclidean formula. The same distances were estimated using an alternative formula of reverse cosine from correlation. It was determined: 1) subgroups of ecological risk and adaptive norm have peculiarities of phenotypical combinations; 2) the number of reliable correlations between phenotypes 2.5 times exceeded that in the adaptive part of the population as compared with unfavourable cohort (according to the health status); 3) the proportion of attractably connected phenotypes (the effect of their interaction) was higher than the corresponding repulsive connected ones (the effect of their repulsion) in the adaptive standard subgroup; 4) on the contrary, the proportion of repulsively connected phenotypes prevailed over the corresponding attractably associated ones in the ecological risk subgroup; 5) there were smaller genetic distances between phenotypes in the adaptive norm subgroup as compared with those in the ecological risk cohort. All these data permit to narrate about considerably greater functional balance of the studied portion of the genome in the clinically healthy subgroups that express the display of genetic homeostasis in complex discrete nonlinked characters. Interloci correlation between Hp and Cerumen systems in the three populations studied were obtained.

[Study of interlocus interactions using a series of gene markers in samples from groups with different health status]. / Izuchenie mezhlokusnykh vzaimodeistvii po serii genov-markerov v vyborkakh s razlichnym urovnem zdorov'ia.

Interloci equilibrium between pairs of gene markers in the samples of different health rate in the population of Buryats of Chitinskaya Province was tested. The following methods were used: calculation of interloci correlation coefficients, chi 2-testing of the hypothesis of interloci equilibrium and the modification of principal components analysis on the basis of the matrix of Pearson's coefficients of contingency. In the groups of "extreme" health rate the tendency to increase in interloci disequilibrium was discovered. The reason for this effect is the increase in some phenotype combination frequencies that can be considered as markers of non-specific individual resistance in the environmental condition of the populations studied.

[An ecogenetic approach to studying the adaptation and human health]. / Ekogeneticheskii podkhod k issledovaniiu adaptatsii i zdorov'ia cheloveka.

Genetic markers--blood groups ABO, RH, MN; serum proteins HP, PI, TF, C3; erythrocyte enzymes ACP1, ESD, AK1, PGM1, GLO1, PGD, PGP; and the other: PTC-tasting, ear wax types and color vision, were studied in two aboriginal Buryatian populations of Baikal Lake region: in Chitinskaya and Irkutskaya Provinces. Two samples were further divided into subgroups, according to their health status: "healthy", "indefinite" and "sick" by means of special regression procedure. The "healthy" subgroup of the Chitinskaya Province population is characterized by higher frequencies of PTC-tasters: 0.871 vs. 0.757 in the "sick" part (chi 2 = 5.36, p less than 0.05); higher frequency of the phenotype PI M1M1: 0.734 in "healthy" vs. 0.547 in "sick" (chi 2 = 8.89, p less than 0.01); also, lower frequency of the PI M1M2 phenotype: 0.148 and 0.299, respectively (chi 2 = 7.49, p less than 0.01); the frequencies of the phenotype TF C2C2 are: 0.015 and 0.076 (chi 2 = 5.48, p less than 0.05). In Irkutskaya Province population differences between "healthy" and "sick" subgroups were discovered for blood group AB: "healthy" 0.046 and "sick"--0.175 (chi 2 = 11.28, p less than 0.010); for GC (1F-2)--0.214 and 0.116 (chi 2 = 4.45, p less than 0.05). Some other differences between "healthy" and "sick" in both populations are not significant. Some trends concerning heterozygosity in loci--GC, PGM, TF were discovered. The results are considered from the viewpoint of higher fitness of some genetic traits in the populations studied.

[Genetic polymorphism of the erythrocytic enzymes in the Buryat populations]. / Geneticheskii polimorfizm éritrotsitarnykh fermentov v populiatsiiakh Buriat.

Polymorphism of seven erythrocytic enzymes PGM1, ESD, CLO1, PGD and PGP were studied in five samples of Buryats. The main investment into differentiation between populations has been made by the following systems: CLO1, PGD and PGM1. Analysis of genetic distances between populations demonstrated that there was some parallelism among the genetic and anthropological differentiation in the Buryat populations. The groups of the Agingsky county (the area to the east from the Baikal Lake) have probably the largest proportion of the Caucasian genes as compared to other populations studied. One of the characteristics of the Buryats, especially for the population to the east from the Baikal Lake, is high frequency of the PGD allele. The rate of the genetic variability on the intra-population level is higher than the difference between populations. This means that the divergence between the Buryats populations is not very strong. Consideration of the genetic variability on the intra-population level seems to be more perspective for ecogenetic estimation of the adaptive genetic processes than analysis of the differences between populations studied.

[Possibilities of creating a genetic information database and its processing based on the DBase3-plus system]. / Vozmoshnosti sozdaniia bazy dannykh genetichskoi informatsii i ee obrabotki na osnove subd DBase3-plus.

Special methods are required for computing information on biological objects under complex research. The DBASE3-PLUS system offers vast possibilities for working with large quantity of different sets of information in multi-aspect statistical analysis. Usefulness of this system for creation of and operation with the data on distribution of genetic and non-genetic traits in a population was shown by means of the special set of applied programs in the dBase language. An example presented is aimed at distinguishing those genetic markers which probably can influence common individual health of the members of population. Special regression procedure was suggested to divide the population sample into subgroups with different health levels. Significant differences in distribution of some genetic markers were demonstrated between healthy persons and those who were suffering from chronic diseases.