RESUMO
Following anterior cruciate ligament (ACL) reconstruction with the middle third part patellar tendon, the graft undergoes histological rearrangement due to biomechanical action, which transforms it into a structure similar to the normal ACL. The purpose of our study was to make a qualitative and quantitative histological evaluation, by transmission electron microscopy (TEM), of the neoligamentization process of a bone-patellar tendon-bone (BTPB) graft used as pro-ACL at different follow-up times. We analysed the ultrastructure of collagen fibrils by focusing on their size and distribution with respect to a normal patellar tendon and a normal ACL used as controls. Our results showed that up to 24 months follow-up, progressive ultrastructural changes towards the normal ACL were observed. At longer times after surgery (48 and 120 months) no further changes were evident and the ultrastructure showed a marked reduction in large fibrils, which was typical of the control patellar tendon, and a significant increase in small fibrils. The ultrastructure seemed to combine fibrils from two different morphological units. The BPTB graft used as ACL underwent a transformation process for up to two years. After that period the transformation ceased and for ten years failed to reach the ultrastructural aspect of a normal ACL. However, from an architectural point of view the graft was slowly transformed into a structure similar to ACL with respect to the different mechanical stresses the ligament has to sustain.
Assuntos
Ligamento Cruzado Anterior/cirurgia , Enxerto Osso-Tendão Patelar-Osso/patologia , Ligamento Patelar/ultraestrutura , Adolescente , Adulto , Lesões do Ligamento Cruzado Anterior , Estudos de Casos e Controles , Colágeno/ultraestrutura , Tecido Elástico/ultraestrutura , Elastina/ultraestrutura , Proteínas da Matriz Extracelular/ultraestrutura , Seguimentos , Humanos , Masculino , Microscopia Eletrônica de TransmissãoRESUMO
In this study we investigated the presence and localization of thyroxine in Ciona intestinalis larvae and its involvement in metamorphosis. To date, the mechanisms regulating the metamorphosis of ascidians remain largely unknown. In vivo treatment of swimming larvae with exogenous L-thyroxine and thiourea, and in vitro experiments utilizing high performance liquid chromatography, radioimmunoassay, and immunoperoxidase staining demonstrate the presence of thyroxine at the larval stage. This suggests that this hormone may participate in the control of metamorphosis and thus play a different role from that observed in adults.
Assuntos
Metamorfose Biológica/fisiologia , Tiroxina/farmacologia , Urocordados/crescimento & desenvolvimento , Animais , Cromatografia Líquida de Alta Pressão , Larva/crescimento & desenvolvimento , Radioimunoensaio , Natação , Tiroxina/análiseRESUMO
The authors review a consecutive series of 7 pharyngo-oesophageal diverticula surgically treated from 1979 to 1994. In their opinion, surgery of diverticula to be effective must be preceded by dynamic and functional studies to outline the motor impairment often associated. Surgical indications and the different surgical techniques are also emphasized.