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1.
ACS Sens ; 9(1): 217-227, 2024 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-38165082

RESUMO

Acetylene (C2H2) is a gas that can cause explosions in transformers even at low concentrations. Gas chromatography (GC) or photoacoustic spectroscopy (PAS) have been used to detect C2H2 during dissolved gas analysis (DGA), but they are not suitable for monitoring numerous transformers at substations. Even though metal oxide semiconductor (MOS) based C2H2 sensors have drawn much attention as a potential solution, existing MOS-based C2H2 sensors have low sensitivity toward C2H2 in the transformer environment (<2% O2 concentrations). This study develops high-performance C2H2 gas sensors for DGA using a heterostructure of CuO/ZnO (CZ) via the electrospinning process. Performance of various ratios of CZ composite nanofibers are compared in a transformer-like environment, and the optimal composition of CZ nanofibers for detection of C2H2 at 2% O2 concentration is proposed. The CuO:ZnO = 8:2 (CZ2) sensor achieves the highest response (Rg/Ra = 7.6 against 10 ppm of C2H2) toward low concentration of C2H2 at 200 °C with good stability (>10 h). In addition, the CZ2 sensor also shows a high selectivity (>5 times) to coexisting transformer oil gases which are H2, CH4, C2H4, C2H6, CO, and CO2. Overall, this study is the first to demonstrate a high performing DGA sensor under 2% O2 concentration that can provide a practical solution to monitoring the low concentration of C2H2 in transformers effectively.


Assuntos
Nanofibras , Óxido de Zinco , Acetileno , Fontes de Energia Elétrica , Gases , Óxidos
2.
PLoS One ; 17(2): e0264026, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35176091

RESUMO

Disease caused by Pepper yellow leaf curl virus (PepYLCV) is one of the greatest threats to pepper (Capsicum spp.) cultivation in the tropics and subtropics. Resistance to PepYLCV was previously identified in a few Capsicum accessions, but no resistance QTLs have been mapped. This study aimed to elucidate the genetics of PepYLCV resistance in C. annuum L. Augmented inoculation by the viruliferous whitefly Bemisia tabaci was used to evaluate parental lines and an F2 segregating population derived from a cross between resistant C. annuum line LP97 and susceptible C. annuum line ECW30R. Final evaluation was performed six weeks after inoculation using a standardized 5-point scale (0 = no symptoms to 4 = very severe symptoms). A high-density linkage map was constructed using genotyping-by-sequencing (GBS) to identify single-nucleotide polymorphism (SNP) markers associated with PepYLCV resistance in the F2 population. QTL analysis revealed three QTLs, peplcv-1, peplcv-7, and peplcv-12, on chromosomes P1, P7, and P12, respectively. Candidate genes associated with PepYLCV resistance in the QTL regions were inferred. In addition, single markers Chr7-LCV-7 and Chr12-LCV-12 derived from the QTLs were developed and validated in another F2 population and in commercial varieties. This work thus provides not only information for mapping PepYLCV resistance loci in pepper but also forms the basis for future molecular analysis of genes involved in PepYLCV resistance.


Assuntos
Begomovirus/fisiologia , Capsicum/genética , Cromossomos de Plantas/genética , Resistência à Doença/genética , Doenças das Plantas/genética , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Capsicum/imunologia , Capsicum/virologia , Mapeamento Cromossômico , Resistência à Doença/imunologia , Genótipo , Doenças das Plantas/imunologia , Doenças das Plantas/virologia
3.
Clin Exp Emerg Med ; 3(4): 197-203, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28168226

RESUMO

OBJECTIVE: The aim of this study was to evaluate the effect of adding bedside ultrasonography to the diagnostic algorithm for nephrolithiasis on emergency department (ED) length of stay. METHODS: A prospective, randomized, controlled pilot study was conducted from October 2014 to December 2014 with patients with acute flank pain. In the non-ultrasonography group (NUSG), non-contrast computed tomography was selected based on clinical features and hematuria in the urinalysis. In the ultrasonography group (USG), non-contrast computed tomography was selected based on clinical features and hydronephrosis on bedside ultrasonography. The primary outcome was ED length of stay. The secondary outcomes were radiation exposure, amount of analgesics, proportion of patients with diseases other than ureteral calculus, and proportion of patients with unexpected ED revisits within 7 days from the index visit. RESULTS: A total of 103 patients were enrolled (NUSG, 51; USG, 52). The ED length of stay for the USG (89.0 minutes) was significantly shorter than that for the NUSG (163.0 minutes, P<0.001). There were no significant differences between the two groups in the radiation exposure dose (5.29 and 5.08 mSv, respectively; P=0.392), amount of analgesics (P=0.341), proportion of patients with diseases other than ureteral calculus (13.0% and 6.8%, respectively; P=0.486), and proportion of patients with unexpected ED revisits within 7 days from the index visit (7.8% and 9.6%, respectively; P=1.000). CONCLUSION: The use of early bedside ultrasonography for patients with acute flank pain could reduce the ED length of stay without increasing unexpected ED revisits.

4.
Clin Exp Emerg Med ; 2(2): 89-94, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27752578

RESUMO

OBJECTIVE: The aim of this study was to evaluate the effectiveness of teaching A- and B-lines, and lung sliding with a novel simulation methods using hand ultrasound. METHODS: All subjects enrolled were medical school students who were novices in lung ultrasound. All subjects attended a 20-minute lecture about lung ultrasound using simulated video clips of A-lines, B-lines, and lung sliding; and then a 20-minute post-test was administered. The post-test included questions on the presence or absence of A-lines, B-lines, and lung sliding using a random mixture of 20 real video clips and 20 simulated video clips created by using hand ultrasound with or without foam dressing materials. A Wilcoxon signed rank test was used to compare the scores of A-lines, B-lines, and lung sliding between the real images (RG) and simulated models (SG). RESULTS: There was a statistically significant difference in the median score of the correct answers for A-lines (RG, 18; SG, 17; P=0.037). Correct answers for B-line were significantly different between RG and SG group (RG, 18; SG, 17; P=0.008). There was a statistically significant difference in the median score of the correct answers for lung sliding (RG, 16; SG, 18; P<0.001). CONCLUSION: We found this novel B-line teaching model by using a hand ultrasound with a wet foam dressing material is effective for beginners who are less experienced with lung ultrasound and pulmonary interstitial syndrome.

5.
J Forensic Sci ; 58(1): 179-83, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23066880

RESUMO

The objectives of our study were to investigate the dose-response relationship of the TASER X26 discharge duration in an anesthetized swine model. Fourteen swines were anesthetized and then exposed to TASER X26 discharge for 5 sec (n = 5) or for 10 sec (n = 6). The sham control group (n = 3) was anesthetized and studied using the same protocol except TASER X26 discharges during the experiments. Hemodynamic parameters were obtained. Blood pressure and total peripheral resistance decreased significantly after TASER discharge and returned to baseline value at 15 min after 5 sec of TASER discharge but did not return to baseline values during the 30-min observation period after 10 sec of TASER discharge. Repetitive TASER X26 discharge resulted in adverse physiologic events with a dose-response relationship related to the duration of TASER X26 discharge in an anesthetized swine model.


Assuntos
Estimulação Elétrica/instrumentação , Anestésicos/administração & dosagem , Animais , Bicarbonatos/sangue , Pressão Sanguínea , Débito Cardíaco , Combinação de Medicamentos , Frequência Cardíaca , Concentração de Íons de Hidrogênio , Ácido Láctico/sangue , Modelos Animais , Consumo de Oxigênio , Suínos , Tiletamina/administração & dosagem , Resistência Vascular , Zolazepam/administração & dosagem
7.
Clin Toxicol (Phila) ; 50(3): 183-8, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22372785

RESUMO

CONTEXT: Some patients with acute carbon monoxide poisoning will experience delayed neurological sequelae. Several factors associated with delayed neurological sequelae have been reported, but these factors are unsatisfactory for the assessment of unconscious patients. OBJECTIVE: The aim of this study was to assess the usefulness of the serum S100B protein as a biochemical marker for predicting delayed neurological sequelae. MATERIALS AND METHODS: In this retrospective study, we evaluated the data for patients who visited an emergency medical center once during a period of 7 months. The enrollment criteria were the diagnosis of acute carbon monoxide poisoning and the measurement of the serum S100B level. A standardized extraction using medical records was performed. RESULT: A total of 71 patients were enrolled, and 10 patients (14.1%) presented delayed neurological sequelae. The delayed neurological sequelae group had a longer duration of carbon monoxide exposure, a longer duration of loss of consciousness, and a worse mental status (p-value < 0.001). In addition, the S100B protein levels were higher in the delayed neurological sequelae group (0.891 vs. 0.063, p-value < 0.001). Multiple logistic regression analysis showed that only the serum S100B protein level was independently associated with the development of delayed neurological sequelae (OR, 120.594; 95% CI, 4.194-3467.220), and a serum S100B protein level of more than 0.165 µg/L predicted the development of delayed neurological sequelae (sensitivity 90%, specificity 87%). DISCUSSION AND CONCLUSION: In the present study, the level of serum S100B protein was found to be useful for evaluating acute CO poisoning patients and was found to be an independent predictor of the development of DNS after acute CO poisoning.


Assuntos
Intoxicação por Monóxido de Carbono/complicações , Fatores de Crescimento Neural/sangue , Doenças do Sistema Nervoso/etiologia , Proteínas S100/sangue , Doença Aguda , Adulto , Intoxicação por Monóxido de Carbono/sangue , Intoxicação por Monóxido de Carbono/diagnóstico , Carboxihemoglobina/análise , Feminino , Hospitais Universitários , Humanos , Modelos Logísticos , Masculino , Doenças do Sistema Nervoso/sangue , Doenças do Sistema Nervoso/diagnóstico , Valor Preditivo dos Testes , Prognóstico , Curva ROC , Estudos Retrospectivos , Subunidade beta da Proteína Ligante de Cálcio S100
8.
South Med J ; 102(3): 239-47, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19204614

RESUMO

OBJECTIVE: This study evaluates the effectiveness of mobile discharge instruction videos (MDIVs) in communicating discharge instructions to patients with lacerations or sprains. METHOD: A prospective controlled study was performed on patients with lacerations or sprains in a quaternary emergency center from April 1, 2008 to May 31, 2008. Upon discharge, patients were systematically allocated to receive printed discharge instructions (PDIs) or MDIVs. Within 48 hours of the patients' discharge, a standard questionnaire was provided via telephone to evaluate the patients' comprehension of, convenience rating for, and satisfaction with their given discharge instructions. RESULTS: Of the 645 patients with lacerations or sprains during the study period, a convenience sample of 161 patients was enrolled in the study; 77 in the PDIs group (the P group) and 84 in the MDIVs group (the M group). There were no statistically significant differences in the ages, genders, and levels of education of the subjects in the two groups. The mean of the correct answers on wound care in the questionnaire was 2.7 +/- 0.7 in the M group and 2.4 +/- 0.8 in the P group (P < 0.05). The convenience rating was 85.7% in the M group and 63.6% in the P group (P < 0.05). The rate of satisfaction was 90.5% in the M group and 90.9% in the P group (P > 0.05). CONCLUSION: The mobile discharge instruction video (MDIV) improved the communication of discharge instructions. Further studies will be needed to explore the actual compliance of patients to treatment regimens.


Assuntos
Lacerações/terapia , Cooperação do Paciente , Educação de Pacientes como Assunto/métodos , Autocuidado , Entorses e Distensões/terapia , Gravação em Vídeo , Adulto , Telefone Celular , Criança , Serviços Médicos de Emergência , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pais , Alta do Paciente , Satisfação do Paciente
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