Shift in ectomycorrhizal community composition in Scots pine (Pinus sylvestris L.) seedling roots as a response to nickel deposition and removal of lichen cover.

Scots pine seedlings were exposed to wet-deposited nickel (Ni) and removal of lichen cover in a dry heath Scots pine forest. Ni deposition affected the colonization of roots by indigenous ectomycorrhizal fungi in contrasting ways in intact and skimmed quadrats. Highest frequencies of tubercle morphotypes of ectomycorrhiza were found in quadrats exposed to 100 mg m(-2) year(-1) Ni in lichen covered treatment, while in skimmed quadrats these peaked after the treatment with 10 mg Ni m(-2) year(-1). Removal of the lichen layer increased the value of diversity index (H') of ectomycorrhizal fungal community, probably due to the increase in the evenness of the morphotype distribution. Lichen removal seemed also to improve the condition of the short roots, as the frequencies of poor and senescent short roots were decreased by the removal.

Urban polluted forest soils induce elevated root peroxidase activity in Scots pine (Pinus sylvestris L.) seedlings.

Plant biomass. mycorrhizal status and root peroxidase activity were measured in ectomycorrhizal Scots pine (Pinus sylvestris L.) seedlings grown in urban polluted and native, non-polluted forest soils with added ammonium or potassium sulphates simulating N and S deposition of urban areas. Peroxidase activity in the fine roots of seedlings planted in polluted forest soils was higher than in those planted in non-polluted soils and correlated positively with the activities measured in an earlier study in the roots of mature Scots pines growing at the sites from where the soils were collected. Growth of seedlings and mycorrhizal status were not affected by the origin of soil. Exposing the seedlings to winter acclimation conditions for 6 weeks elevated peroxidase activity in the roots. The addition of ammonium or potassium sulphate to non-polluted soils did not induce elevated root peroxidase activity, although at the levels of 0.5 and 1.0 g of ammonium sulphate a slight increasing trend was observed. We suggest, that indirect biotic factors, i.e. changes in the community structure of soil fungi, early stages of recognition, and defence reactions of pine roots against saprophytic and pathogenic fungi may be participating in the elicitation of peroxidase (POD) activity, although the possible role of heavy metals cannot be excluded.

Scots pine responses to CO2 enrichment--I. Ectomycorrhizal fungi and soil fauna.

Ectomycorrhizal Scots pine seedlings were grown in unfertilized forest soil at ambient and double (ca 700 ppm) atmospheric concentrations of CO2. The biomass of seedlings and fungal biomass both in the roots and in the soil and the numbers of certain groups of soil animals were measured under summer conditions and after an artificial winter acclimation period. No biomass parameter showed any significant change due to CO2 elevation. Increases were found during the winter acclimation period in total and fine root biomasses, fungal biomass in the soil and total fungal biomass both in the roots and in the soil, while the ratio of needle biomass: fungal biomass and the shoot: root ratio decreased. The N concentration in previous-year needles was lower in the double CO2 environment than with ambient CO2. Enchytraeids almost disappeared in the double CO2 environment during winter acclimation, while the numbers of nematodes increased at the same time in both treatments.

Estimates of fungal biomass in Scots pine stands on an urban pollution gradient.

Biomasses of ectomycorrhizal and saprotrophic fungal communities partitioned into sporophores and non-reproductive structures were estimated in mature Scots pine (Pinus sylvestris L.) stands along an urban nitrogen and sulphur pollution gradient in northern Finland. The average total biomass of fungi varied in the four pollution zones from 14.6 to 20.2 g d. Wt kg-1 soil d. wt and from 73.3 to 108.0 g d. Wt m-2 , the mycelia of both mycorrhizai and saprotrophic fungi in the soil comprising 72-80% of the total. The annual carbon allocation to the fungal communities was calculated to vary between 9 and 26% of the estimated annual carbon assimilation at the Scots pine sites. The size of the mean fungal biomass fractions decreased in the following sequence: mycelia in the soil > fungal biomass in fine roots estimated in terms of chitin > sclerotia > fungal biomass in fine roots estimated in terms of ergosterol > sporopbores of mycorrhizal fungi > sporophores of saprotrophie fungi. A positive correlation was obtained between the number of Scots pine mycorrhiza and the average sporophore yield of mvcorrhiaal fungi for three successive years. Tbe sporophore biomass of the mycorrhizal fungi was smaller at the most polluted than at the least polluted sites. The total fungal biomass allocation was not affected by urban pollution.