Bioactive and Hemocompatible PLA/Lignin Bio-Composites: Assessment of In Vitro Antioxidant Activity for Biomedical Applications.

In this study, acetylated soda lignin (ASL) and non-acetylated soda lignin (SL) were extruded with PLA in different concentrations to fabricate antioxidant polylactic acid (PLA)/lignin composites for potential biomedical applications. After lignin acetylation, good compatibility was observed between PLA and lignin in scanning electron microscopy images. All the PLA/ASL composites displayed higher mechanical properties than PLA/SL composites. PLA/ASL5 displayed the highest mechanical characteristics with elongation at break of 10% and tensile strength of 57 MPa, while PLA/SL15 and PLA/SL20 demonstrated superior UV-blocking potential with UV transmittance less than 10%. Addition of ASL in PLA lead to an increase in the hydrophobic character, with all the PLA/ASL displaying a higher water contact angle. The antioxidant test using 2,2-diphenyl-1-picrylhydrazyl assay showed that PLA/SL composites rendered superior radical scavenging activity (RSA), with PLA/SL20 composites displaying an RSA of 80%. Furthermore, in vitro antioxidant activity and cytocompatibility were analyzed using human colon cancer cells (HCT-15) and gastric epithelial cells (NCC-24). In vitro antioxidant activity, evaluated by H2O2 exposure was confirmed by a live/dead assay. PLA/SL composites protected both types of cells from oxidative stress. In addition, all PLA/SL and PLA/ASL composites promoted cell proliferation compared to PLA. PLA/SL5 and PLA/SL10 displayed the highest cell proliferation of all composites. Lastly, all PLA/SL and PLA/ASL composites had a hemoglobin release less than 2%. The antioxidant properties, cytocompatibility, and hemocompatibility of lignin/PLA demonstrated in our study indicate that these lignin/PLA composites possess the desirable attributes for potential biomedical applications.

A study on activation mechanism in perspective of lignin structures and applicability of lignin-derived activated carbons for pollutant absorbent and supercapacitor electrode.

In this study activated carbons were produced from the biorefinery waste lignin (Asian lignin (AL) USA & Inbicon lignin (IL) Denmark) to evaluate their potential in waste water treatment and as energy storage devices. These products were studied for their surface characteristics as a function of reaction temperature, time, and catalyst loading accordingly. Under the conditions with a temperature lower than 750 °C and within a reaction time of 1 h, the catalytic reaction of alkali-carbon bonding occurred from the external surface, and a turbostratic disorder structure with a large aromatic ring system was formed. More severe reaction conditions accelerated the volatile release of de-alkylated aromatics such as benzene and naphthalene, along with structure and surface collapse. The maximum BET surface area of 2782 m2/g was obtained at 750 °C, 2 h and catalyst ratio of 4. Lignin-derived activated carbon was more efficient for the removal of organic pollutants (<50% adsorption capacity) rather than heavy metals (adsorption capacity >90%) due to interaction of π-π bonding. Furthermore, the activated carbon has a potential to be used as a supercapacitor electrode with high specific capacitance (214.0 F/g AL lignin) and an excellent cyclic stability (95% of their initial capacity). The results of this study demonstrate that lignin is an attractive precursor to produce activated carbons with diverse applications both as biosorbent and as a carbon electrode material even so with acceptable performance.