[Open abdominal trauma with inferior vena cava injury]. / Sluchai sochetannoi otkrytoi travmy zhivota s povrezhdeniem nizhnei poloi veny.

The authors report surgical treatment of a patient with a penetrating abdominal trauma followed by damage to inferior vena cava, liver, duodenum and pancreas, massive bleeding and large retroperitoneal hematoma. Suturing of inferior vena cava defect, liver and pancreas, Billroth II gastric resection, drainage of retroperitoneal space and abdominal cavity were carried out. Postoperative period was complicated by pneumonia and seroma in lesser sac that required percutaneous puncture. Antibiotic therapy was used postoperatively. The patient was discharged in 25 days after surgery.

[Molecular characteristics of the chitin-binding peroxidases of plants].

The chitin-binding ability of isoperoxidases isolated from 23 plants of different species was studied. The activation of peroxidases in a protein extract in the presence of this polysaccharide was found for 14 of the studied plants. Anionic isoperoxidases were shown to be sorbed on chitin and eluted from them with 1 M NaCl for 16 of the plant species. Cationic isoforms of the peroxidases of some species of the Fabaceae and Cucurbitaceae plant families also bound to chitin. An immunochemical similarity was found between the chitin-binding isoperoxidases of taxonomically distant plant species (the Pomaceous, Fabaceae, and gourd families). Moreover, a high homology of the molecular structures of the polysaccharide-binding sites was revealed for the anionic peroxidases of rice, wheat, oat, zucchini, cucumber, and radish. We propose the existence of a special class of plant peroxidases that bind with polysaccharides (chitin) and participate in the protective reactions of plants against pathogens.

Kininase of the Latrodectus tredecimguttatus venom: a study of its enzyme substrate specificity.

The specificity of the peptide hydrolyzing action of a highly purified preparation of kininase from Latrodectus Tredecimguttatus venom was studied by the method of TLC on silica gel with the use of various synthetic peptides as substrates. It was shown that the enzyme cleaves the -Pro(7)-Phe(8)-bonds in BK and AI molecules liberating, correspondingly, the C-terminal dipeptide and tripeptide. Exopeptidase specificity was not revealed in the enzyme activity with the use of a number of free and N-substituted tri- and pentapeptides. The results obtained characterize the spider venom kininase as a thiol endopeptidase, which cleaves internal peptide bonds at the proline carboxyl end.

[Kininase from the Latrodectus tredecimguttatus venom. Characteristics of the enzyme as a thiol endopeptidase hydrolyzing the -Pro7-Phe8- bond of bradykinin]. / Kininaza iada pauka Latrodectus tredecimguttatus. Kharakteristika fermenta kak tiolovoi éndopeptidazy, rasshchepliaiushchei sviaz' -Pro7-Phe8- v bradikinine.

The nature of the bradykinin (BK)-hydrolyzing (kininase) activity of peptidhydrolase isolated from spider (Latr. tredecimguttatus) venom has been studied. It was found that the BKase activity of the enzyme is fully inhibited by organic mercurials (10(-5)-10(-6) M) as well as by 5,5'-dithiobis(2-nitrobenzoic acid) (10(-7) M); the latter blocks three SH-groups within the enzyme molecule. Serine and metalloproteinase inhibitors have no effect on the kininase activity. Thin-layer chromatography on silicagel revealed that the highly purified enzyme hydrolyzes the -Pro7-Phe8- bond of BK liberating the C-terminal dipeptide, HPhe-ArgOH. Besides, the kininase splits off the C-terminal tripeptide from angiotensin I by hydrolyzing its -Pro7-Phe8-bond. The enzyme does not exhibit any exopeptidase activity with free and N-substituted tri- and pentapeptides. The data obtained suggest that the Latr. tredecimguttatus kininase can be related to thiol endopeptidases hydrolyzing the peptide bonds formed by proline carboxyl.

[Structural-functional characteristics of vasoactive peptides from the Vespa orientalis venom]. / Strukturno-funktsional'naia khrakteristika vazoaktivnykh peptidov iz iada shershnia Vespa orientalis.

Two vasoactive peptides are isolated from the Vespa orientalis venom by gel filtration and ion-exchange chromatography. The physicochemical and functional properties of peptides are studied. Vasoactive peptides show the myotropic activity and hypotensive action which is of prolonged character as compared with bradykinin. Their complete amino acidic sequences are determined. One of the peptides is a similar structural analogue of bradykinin.

[Effect of chronic gamma irradiation on the protein composition and cholesterol content of the blood of dogs]. / Vliianie khronicheskogo gamma-oblucheniia na belkovyi sostav i soderzhanie kholesterina v krovi sobak.

In a chronic experiment dogs were exposed to irradiation in doses of 190, 360 and 560 rad for 3 years. As compared to the unirradiated controls they showed a slower recovery of the albumin and cholesterol content in the blood. The cholesterol content varied, depending on the dose of chronic irradiation, whereas the protein content did not show such a correlation.

[Changes in the sugar content in the blood of dogs during 6 years of chronic gamma irradiation]. / Izmeneniia soderzhaniia sakhara v krovi u sobak pri 6-letnem khronicheskom gamma-obluchenii.

The paper presents the results of a many-year investigation of the sugar content in dogs irradiated with total doses of 125, 370, 720, 750, 1130 rad. During the first year the sugar content tended to increase and later to decrease. Irradiated and untreated animals showed different reactions to additional loads (running, thermal exposure, acute irradiation). The differences included the direction and level of changes in the sugar content. There was no correlation between the changes and the dose rate. The changes returned to normal after termination of the exposure.