Expression of M30 and M65 in celiac disease. Analytical cross-sectional study

ABSTRACT BACKGROUND: The role of villous atrophy in apoptosis, a distinctive feature of celiac disease, is a matter of controversy. The aim of this study was to determine the apoptosis rate through immunohistochemical staining for M30 and M65 in celiac disease cases. DESIGN AND SETTING: Analytical cross-sectional study in a tertiary-level center. METHODS: Duodenal biopsies from 28 treatment-naive patients with celiac disease, 16 patients with potential celiac disease, 10 patients with a gluten-free diet and 8 controls were subjected to immunohistochemical staining for the end-apoptotic marker M30 and the total cell death marker M65. H-scores were compared. Several laboratory parameters were recorded concomitantly, and at the one-year follow-up for celiac disease and potential celiac disease patients. RESULTS: There was a significant difference in H-score for M30 expression between the celiac disease, potential celiac disease and gluten-free diet groups (P = 0.009). There was no significant difference in H-score for M65 expression. There was a positive correlation between the H-score for M30 expression and the anti-tissue transglutaminase immunoglobulin A (anti-tTgIgA) and anti-tissue transglutaminase immunoglobulin G (anti-tTgIgG) levels (R = 0.285, P = 0.036; and R = 0.307, P = 0.024, respectively); and between the H-score for M65 expression and the anti-tTgIgA and anti-tTgIgG levels (R = 0.265, P = 0.053; and R=0.314, P = 0.021, respectively). There was no difference between celiac disease and potential celiac disease patients regarding the laboratory parameters selected. CONCLUSION: The rates of apoptosis and nutritional deficiencies in patients with potential celiac disease were similar to those in patients with celiac disease.

Expression of M30 and M65 in celiac disease. Analytical cross-sectional study.

BACKGROUND: The role of villous atrophy in apoptosis, a distinctive feature of celiac disease, is a matter of controversy. The aim of this study was to determine the apoptosis rate through immunohistochemical staining for M30 and M65 in celiac disease cases. DESIGN AND SETTING: Analytical cross-sectional study in a tertiary-level center. METHODS: Duodenal biopsies from 28 treatment-naive patients with celiac disease, 16 patients with potential celiac disease, 10 patients with a gluten-free diet and 8 controls were subjected to immunohistochemical staining for the end-apoptotic marker M30 and the total cell death marker M65. H-scores were compared. Several laboratory parameters were recorded concomitantly, and at the one-year follow-up for celiac disease and potential celiac disease patients. RESULTS: There was a significant difference in H-score for M30 expression between the celiac disease, potential celiac disease and gluten-free diet groups (P = 0.009). There was no significant difference in H-score for M65 expression. There was a positive correlation between the H-score for M30 expression and the anti-tissue transglutaminase immunoglobulin A (anti-tTgIgA) and anti-tissue transglutaminase immunoglobulin G (anti-tTgIgG) levels (R = 0.285, P = 0.036; and R = 0.307, P = 0.024, respectively); and between the H-score for M65 expression and the anti-tTgIgA and anti-tTgIgG levels (R = 0.265, P = 0.053; and R=0.314, P = 0.021, respectively). There was no difference between celiac disease and potential celiac disease patients regarding the laboratory parameters selected. CONCLUSION: The rates of apoptosis and nutritional deficiencies in patients with potential celiac disease were similar to those in patients with celiac disease.

Identification of interfacial stress pattern on dentin post surface

OBJECTIVE: The purpose of this study was to develop an interfacial stress distribution model for the post -dentin interface due to 50, 100 and 250 Newton occlusal biting forces by utilizing a finite element model. The hypothesis of the present study is that, on the basis of these interfacial stress models for post-dentine, it is possible to compute interfacial stresses at any given point on the post dentin surface and capture the characteristic behavior of post under the specific loading conditions under consideration. MATERIAL AND METHOD: The two-dimensional FEM was created by a self-developed image processing system, manually digitizing the key points on dental cross-section boundaries obtained from an actual maxillary canine tooth image. Crown, post core and coronal-radicular restoration were modeled by creating key points and transferring them to a two-dimensional coordinate XY system in the FE environment. The generated post in the model was 1.4 mm in diameter and 15 mm in length. A finite element (FE) analysis was performed to investigate the interfacial shear stress distribution on the dentine-post interface frequently used for the restoration of endodontically treated teeth. RESULTS AND CONCLUSION: From theresults and within the limitations of the present study it can be concluded that the interfacial stress along the post is nonlinear and is roughly sinusoidal and the peak interfacial stress between the post and the dentin is expected to occur on top of the post. Sinusoidal models were developed to generalize the interfacial stress nonlinearity along the post length. These models can be used for calculation of the interfacial shear stress for 50, 100, and 250 Newton forces applied compressively at 45 degrees angle with respect to the implant axis. This study showed that the interfacial stress along the post was nonlinear and appeared roughly sinusoidal. Regression models were developed to generalize the interfacial stress nonlinearity along...

OBJETIVO: O objetivo deste estudo foi desenvolver um modelo de distribuição do estresse interfacial para a interface pino-dentina devido a forças oclusais (mordida) geradas por 50, 100 e 250 Newton, utilizando um modelo de elementos finitos. A hipótese do presente estudo é que, baseado neste modelo de estresse interfacial (dentina-pino), é possível computar estresses interfaciais em qualquer ponto determinado na interface pino-dentina e capturar o comportamento característico do pino submetido a condições de carga diversas. MATERIAL E MÉTODO: o FEM bidimensional foi criado por um sistema de processamento de imagem autodesenvolvido, digitando-se manualmente os pontos-chave nos limites das seções obtidos de uma imagem real de dente canino. A coroa, o pino e a restauração radicular coronal foram moldados criando pontos-chave e transferindo-os para uma coordenada bi-dimensional (sistema XY) no ambiente FE. O pino gerado no modelo foi de 1.4 mm de diâmetro e 15 mm de comprimento. Uma análise de elementos finitos foi efetuada para investigar a distribuição do estresse de deslizamento na interface dentina-pino, usada com frequência para a restauração de dentes tratados endodonticamente. RESULTADOS E CONCLUSÃO: Pelos resultados e com as limitações do presente estudo, pode ser concluído que o estresse interfacial entre o pino e a dentina é não linear, grosseiramente sinusoidal e o pico de estresse interfacial entre pino e dentina é de ocorrência esperada no topo do pino. Modelos sinusoidais foram desenvolvidos para generalizar o estresse interfacial não linearmente ao longo do comprimento do pino. Estes modelos podem ser usados para cálculo da força de cisalhamento frente a forças de 50, 100, 250 N aplicadas compressivamente em ângulo de 45 graus em relação ao eixo do implante. Este estudo demonstrou que o estresse interfacial ao longo do pino foi não linear, sendo grosseiramente sinusoidal. Modelos de regressão foram desenvolvidos para generalizar o estresse...