Chemical, biochemical, and bioactivity studies on some soda lakes, Wadi El-Natrun, Egypt.

Wadi El-Natrun is one of the most observable geomorphological features in the North-Western Desert of Egypt; it contains several old saline and saline soda lakes. This study investigates physicochemical and biochemical characteristics and estimates the total phenolic content (TPC), total flavonoid content (TVC), and bioactivities of sediment, cyanobacteria, and brine shrimp (Artemia salina) in soda lakes, i.e., El-Hamra Lake 1 (H1) and El-Hamra Lake 2 (H2). These soda lakes are unique extreme ecosystems characterized by high pH (> 9.3), high alkalinity, and salinity. Some extremophilic microorganisms are hosted in this ecosystem. The results revealed that the chemical water type of studied lakes is soda-saline lakes according to the calculated percentage sequence of major cations and anions. Sodium ranked first among major cations with an abundance ratio of e% 58, while chloride came first among anions with an abundance ratio of e% 71, and bicarbonate and carbonate occupied the last rank with an abundance of 6%. The biochemical investigations showed that TPC and TVC are present in concern contents of sediment, cyanobacteria, and brine shrimp (A. salina) which contribute 89% of antioxidant capacity and antimicrobial activities. Thus, this study helps better understand the chemical and biochemical adaptations in soda lake ecosystems and explores natural sources with potential applications in antioxidant-rich products and environmental conservation efforts.

Biosorption of hexavalent chromium and molybdenum ions using extremophilic cyanobacterial mats: efficiency, isothermal, and kinetic studies.

Two extremophilic cyanobacterial-bacterial consortiums naturally grow in extreme habitats of high temperature and hypersaline were used to remediate hexavalent chromium and molybdenum ions. Extremophilic cyanobacterial-bacterial biomasses were collected from Zeiton and Aghormi Lakes in the Western Desert, Egypt, and were applied as novel and promising natural adsorbents for hexavalent chromium and molybdenum. Some physical characterizations of biosorbent surfaces were described using scanning electron microscope, energy-dispersive X-ray spectroscopy, Fourier transformation infrared spectroscopy, and surface area measure. The maximum removal efficiencies of both biosorbents were 15.62-22.72 mg/g for Cr(VI) and 42.15-46.29 mg/g for Mo(VI) at optimum conditions of pH 5, adsorbent biomass of 2.5-3.0 g/L, and 150 min contact time. Langmuir and Freundlich adsorption models were better fit for Cr(VI), whereas Langmuir model was better fit than the Freundlich model for Mo(VI) biosorption. The kinetic results revealed that the adsorption reaction obeyed the pseudo-second-order model confirming a chemisorption interaction between microbial films and the adsorbed metals. Zeiton biomass exhibited a relatively higher affinity for removing Cr(VI) than Aghormi biomass but a lower affinity for Mo(VI) removal. The results showed that these extremophiles are novel and promising candidates for toxic metal remediation.

Even though many researchers worked on the field of metal bioremediation, most use single organism or extracted biogenic materials for heavy metals removal. The novelty of this study is the application of a consortium of cyanobacteria and bacteria from extreme habitats (hyper-salinity, high temperature, harsh weather conditions, high intensity of light and UV light) in the field of environmental safety. This specialized microbial film composed of a diverse group of adapted organisms that co-operate between each other making them more effective bio-remediating agent. This study examined the effectiveness of these consortia as metals bioremediator and cover the gap of research results from the scarce application of novel, cheap and eco-friendly extremophiles in toxic metals removal.

Evaluation of the ecological health and food chain on the shores of four River Nile Islands, Egypt.

This study was conducted to evaluate the impact of human activities on the ecological health and food chain at the shores of four Nile Islands in Great Cairo including El-Qeratten, El-Waraq, El-Zamalek, and El-Manial. Water quality, bacteria, phytoplankton, benthic algae, zooplankton, macrobenthic invertebrates, and bony fishes were examined at each island shore over two seasons including the lowest and the highest flow (February and July 2020, respectively). The obtained results showed that the average values of most of the chemicals in Nile Island's water were within the Egyptian water quality limits. Discriminant analysis of physicochemical factors revealed a similarity between El-Waraq and El-Qerateen and between El-Manial and El-Zamalek. El-Qeratten was the most polluted island. It has the highest total and fecal coliform bacteria count (3.155 and 3.050 Log MPN/100 mL, respectively). El-Zamalek shores have the highest phytoplankton (33,582 cells × 104 L-1) and zooplankton count (310,891 organisms × m-3) and phyto-biochemical values. Biochemical analysis of phytoplankton demonstrated the richness of the bulk by protein (> 85% of biomass), indicating that phytoplankton has a high nutritional value. Elevated zooplankton count was recorded at El-Zamalek, which coincided with the peak of phytoplankton abundance. Mollusca were the dominant macrobenthic invertebrates on most of the island's shores. Bony fishes were represented by 27 species and two crustaceans. The levels of the metals in fish samples were compared with the food safety guideline endorsed by the World Health Organization and Food and Agriculture Organization (WHO/FAO) to evaluate the toxicity level.

Efficiency of extremophilic microbial mats for removing Pb(II), Cu(II), and Ni(II) ions from aqueous solutions.

Two different extremophilic films were used as natural biosorbents to remove Cu(II), Ni(II), and Pb(II) from aqueous solutions. Surface area, scanning electron microscopy imaging, and Fourier transformation infrared spectroscopy were used to characterize the surfaces of the biosorbents. The results indicated high affinity of the biosorbents to remove Pb(II), Cu(II), and Ni(II), with adsorption rates ranging from 73.6 to 100% for both biosorbents. The biosorbents succeed in removing the metal ions from aqueous mixtures in the following order: Pb(II) > Cu(II) > Ni(II). The maximum removal rates of metal ions were achieved at pH 6, contact time of 150 min, biosorbent dose of 2.5 g/L, and metal ion concentration of 50 mg/L. The isothermal studies showed that both Langmuir and Freundlich models well expressed the adsorption process. Kinetically, the pseudo-second-order reaction better expressed the type of reaction than the pseudo-first-order reaction.