Response of human peripheral blood monocyte-derived macrophages (PBMM) to demineralized and decellularized bovine bone graft substitutes.

The performance of apparently biocompatible implanted bovine bone grafts may be compromised by unresolved chronic inflammation, and poor graft incorporation leading to implant failure. Monitoring the intensity and duration of the inflammatory response caused by implanted bone grafts is crucial. In this study, the ability of demineralized (DMB) and decellularized (DCC) bovine bone substitutes in initiating inflammatory responses to peripheral blood monocyte-derived macrophages (PBMMs) was investigated. The response of PBMMs to bone substitutes was evaluated by using both direct and indirect cell culture, reactive oxygen species (ROS) generation, apoptosis, immunophenotyping, and cytokine production. Analysis of DMB and DCC substitutes using scanning electron microscope (SEM) showed a roughened surface with a size ranging between 500 and 750 µm. PBMMs treated with DMB demonstrated cell aggregation and clumping mimicking lipopolysaccharide (LPS) treated PBMMs and a higher proliferation ability (166.93%) compared to control (100%) and DCC treatments (115.64%; p<0.001) at 24h. This was associated with a significantly increased production of intracellular ROS in PBMMs exposed to DMB substitutes than control (3158.5 vs 1715.5; p<0.001) and DCC treatment (2117.5). The bone substitute exposure also caused an increase in percentage apoptosis which was significantly (p<0.0001) higher in both DMB (27.85) and DCC (29.2) treatment than control (19.383). A significant increase in proinflammatory cytokine expression (TNF-α: 3.4 folds; p<0.05) was observed in DMB substitute-treated PBMMs compared to control. Notably, IL-1ß mRNA was significantly higher in DMB (21.75 folds; p<0.0001) than control and DCC (5.01 folds). In contrast, DCC substitutes exhibited immunoregulatory effects on PBMMs, as indicated by the expression for CD86, CD206, and HLDR surface markers mimicking IL-4 treatments. In conclusion, DMB excites a higher immunological response compared to DCC suggesting decellularization process of tissues dampen down inflammatory reactions when exposed to PBMM.

Targeting inflammatory signaling pathways with SGLT2 inhibitors: Insights into cardiovascular health and cardiac cell improvement.

Sodium-glucose cotransporter 2 (SGLT2) inhibitors have attracted significant attention for their broader therapeutic impact beyond simply controlling blood sugar levels, particularly in their ability to influence inflammatory pathways. This review delves into the anti-inflammatory properties of SGLT2 inhibitors, with a specific focus on canagliflozin, empagliflozin, and dapagliflozin. One of the key mechanisms through which SGLT2 inhibitors exert their anti-inflammatory effects is by activating AMP-activated protein kinase (AMPK), a crucial regulator of both cellular energy balance and inflammation. Activation of AMPK by these inhibitors leads to the suppression of pro-inflammatory pathways and a decrease in inflammatory mediators. Notably, SGLT2 inhibitors have demonstrated the ability to inhibit the release of cytokines in an AMPK-dependent manner, underscoring their direct influence on inflammatory signaling. Beyond AMPK activation, SGLT2 inhibitors also modulate several other inflammatory pathways, including the NLRP3 inflammasome, expression of Toll-like receptor 4 (TLR-4), and activation of NF-κB (Nuclear factor kappa B). This multifaceted approach contributes to their efficacy in reducing inflammation and managing associated complications in conditions such as diabetes and cardiovascular disorders. Several human and animal studies provide support for the anti-inflammatory effects of SGLT2 inhibitors, demonstrating protective effects on various cardiac cells. Additionally, these inhibitors exhibit direct anti-inflammatory effects by modulating immune cells. Overall, SGLT2 inhibitors emerge as promising therapeutic agents for targeting inflammation in a range of pathological conditions. Further research, particularly focusing on the molecular-level pathways of inflammation, is necessary to fully understand their mechanisms of action and optimize their therapeutic potential in inflammatory diseases.

Characterization and use of activated carbon synthesized from sunflower seed shell in the removal of Pb(II), Cd(II), and Cr(III) ions from aqueous solution.

In this work, carbon-based nanomaterials such as active carbon which is prepared from common sunflower (Helianthus annuus) seed shell, and the characterization of the activated carbon NPs were studied using FTIR (Fourier transform infrared spectroscopy), XRD, SEM, EDS, and DTA techniques. Activated carbon NPs have been used in the adsorption of Pb(II), Cd(II), and Cr(III) ions from the aqueous phase. The results showed the highest adsorption efficiency was 99.9%, 92.45%, and 98% for Pb(II), Cd(II), and Cr(III) ions respectively at a temperature of 25 °C, pH = 7-9, and a time of 60 and 180 min, in addition to the accordance of the adsorption models for activated carbon with the Freundlich isotherm model at the value of R2 (0.9976, 0.9756, and 0.9907) and Langmuir isotherm model (0.966, 0.999, and 0.9873) of the Pb(II), Cd(II), and Cr(III) ions, respectively. We conclude the possibility of using activated carbon to have an extremely high sorption capacity across the conditions tested, with the highest adsorption efficiency having been >99% for Pb(II), Cd(II), and Cr(III) ions within the pH range 7-9 and a contact time of 60 to 180 min.

Phytic acid effect on periodontal ligament fibroblast: An in-vitro study.

OBJECTIVES: This study evaluated phytic acid (IP6) effect on the viability, alkaline phosphatase (ALP) activity and calcium release of human periodontal ligament (HPDL) cells in optimal (OGL) and elevated glucose level (EGL) in cell culture media. MATERIALS AND METHODS: Cells were seeded in OGL (1000mg/L) or EGL (4500 mg/L) media. IP6 was added at 0.005%, 0.01% or 0.02% concentrations for 24 or 48h, and XTT assay was performed. Cell differentiation and calcium release in presence of 0.02% IP6 in OGL or EGL in non-osteogenic or osteogenic media were analyzed using ALP assay and alizarin red staining, respectively. RESULTS: In OGL, IP6 enhanced the viability of the cells at both exposure times (P<0.05). However, IP6 lowered the viability of the cells with the presence of EGL compared to the control at both exposure times, except for 0.02% IP6 which showed comparable viability to the control at 48 h. In OGL and EGL, ALP activity of the cells was not affected by the presence of IP6 in non-osteogenic media; however, in osteogenic media IP6 lowered the ALP activity. Meanwhile, calcium release was the highest with IP6 within osteogenic media of EGL. CONCLUSIONS: IP6 effects on the HPDL cells were dependent on IP6 concentration, time of exposure, glucose levels and the osteogenic condition of the media. CLINICAL RELEVANCE: This study gives insights on the potential therapeutic effect of IP6 as adjunctive periodontal therapy in patients with diabetes.

Twitter Misinformation Discourses About Vaping: Systematic Content Analysis.

BACKGROUND: While there has been substantial analysis of social media content deemed to spread misinformation about electronic nicotine delivery systems use, the strategic use of misinformation accusations to undermine opposing views has received limited attention. OBJECTIVE: This study aims to fill this gap by analyzing how social media users discuss the topic of misinformation related to electronic nicotine delivery systems, notably vaping products. Additionally, this study identifies and analyzes the actors commonly blamed for spreading such misinformation and how these claims support both the provaping and antivaping narratives. METHODS: Using Twitter's (subsequently rebranded as X) academic application programming interface, we collected tweets referencing #vape and #vaping and keywords associated with fake news and misinformation. This study uses systematic content analysis to analyze the tweets and identify common themes and actors who discuss or possibly spread misinformation. RESULTS: This study found that provape users dominate the platform regarding discussions about misinformation about vaping, with provaping tweets being more frequent and having higher overall user engagement. The most common narrative for provape tweets surrounds the conversation of vaping being perceived as safe. On the other hand, the most common topic from the antivape narrative is that vaping is indeed harmful. This study also points to a general distrust in authority figures, with news outlets, public health authorities, and political actors regularly accused of spreading misinformation, with both placing blame. However, specific actors differ depending on their positionalities. The vast number of accusations from provaping advocates is found to shape what is considered misinformation and works to silence other narratives. Additionally, allegations against reliable and proven sources, such as public health authorities, work to discredit assessments about the health impacts, which is detrimental to public health overall for both provaping and antivaping advocates. CONCLUSIONS: We conclude that the spread of misinformation and the accusations of misinformation dissemination using terms such as "fact check," "misinformation," "fake news," and "disinformation" have become weaponized and co-opted by provaping actors to delegitimize criticisms about vaping and to increase confusion about the potential health risks. The study discusses the mixed types of impact of vaping on public health for both smokers and nonsmokers. Additionally, we discuss the implications for effective health education and communication about vaping and how misinformation claims can affect evidence-based discourse on Twitter as well as informed vaping decisions.

Science Communication as a Collective Intelligence Endeavor: A Manifesto and Examples for Implementation.

Effective science communication is challenging when scientific messages are informed by a continually updating evidence base and must often compete against misinformation. We argue that we need a new program of science communication as collective intelligence-a collaborative approach, supported by technology. This would have four key advantages over the typical model where scientists communicate as individuals: scientific messages would be informed by (a) a wider base of aggregated knowledge, (b) contributions from a diverse scientific community, (c) participatory input from stakeholders, and (d) better responsiveness to ongoing changes in the state of knowledge.

Tiktoking COVID-19 with frontline workers.

Introduction/Objective: During the COVID-19 pandemic, frontline workers have taken to social media platforms to discuss a variety of issues that concern their personal and professional lives. In particular, TikTok's increased prominence as a social media channel has proved significant for enhancing the public presence of healthcare workers and their ability to disseminate content to a wider audience. The ways that healthcare workers use TikTok draws attention to the type of health information disseminated to the public through social media platforms. This provides the public with succinct and often visually entertaining information that may not be otherwise distributed to them directly from elsewhere. This study also provides relevant insights into how social media-TikTok in particular-can be used as a tool for disseminating knowledge about COVID-19 related topics and combatting misinformation by using the credibility of frontline workers. Methods: This study collected a sample of over 2100 TikTok videos posted by healthcare workers that were coded according to the dominant overarching themes. Results: The themes that arose from this sample were: (1) healthcare workers' mental health and working conditions, (2) healthcare heroes/appreciation, (3) criticism against official authorities, (4) countering misinformation, (5) humor/satire, and (6) educational content. Conclusion: Due to the rise in public appreciation for frontline workers, examining the effects of the pandemic through the eyes of frontline workers has drawn attention to their lived realities in various forms. This study provided some insight into how frontline workers use TikTok to disseminate information and education to the public, often relying on their perceived credibility.

SARS-CoV-2-free residual proteins mediated phenotypic and metabolic changes in peripheral blood monocytic-derived macrophages in support of viral pathogenesis.

The large-scale dissemination of coronavirus disease-2019 (COVID-19) and its serious complications have pledged the scientific research communities to uncover the pathogenesis mechanisms of its etiologic agent, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Methods of unveiling such mechanisms are rooted in understanding the viral agent's interactions with the immune system, including its ability to activate macrophages, due to their suggested role in prolonged inflammatory phases and adverse immune responses. The objective of this study is to test the effect of SARS-CoV-2-free proteins on the metabolic and immune responses of macrophages. We hypothesized that SARS-CoV-2 proteins shed during the infection cycle may dynamically induce metabolic and immunologic alterations with an inflammatory impact on the infected host cells. It is imperative to delineate such alterations in the context of macrophages to gain insight into the pathogenesis of these highly infectious viruses and their associated complications and thus, expedite the vaccine and drug therapy advent in combat of viral infections. Human monocyte-derived macrophages were treated with SARS-CoV-2-free proteins at different concentrations. The phenotypic and metabolic alterations in macrophages were investigated and the subsequent metabolic pathways were analyzed. The obtained results indicated that SARS-CoV-2-free proteins induced concentration-dependent alterations in the metabolic and phenotypic profiles of macrophages. Several metabolic pathways were enriched following treatment, including vitamin K, propanoate, and the Warburg effect. These results indicate significant adverse effects driven by residual viral proteins that may hence be considered determinants of viral pathogenesis. These findings provide important insight as to the impact of SARS-CoV-2-free residual proteins on the host cells and suggest a potential new method of management during the infection and prior to vaccination.

Characterizing the Gendered Twitter Discussion of COVID-19 Hoax.

We collected data from Twitter and used content analysis to better understand the gendered discussion around COVID-19 as a hoax. We identified three main categories in the inductive stage of the research: (1) sympathetic to human rights & perceived injustice, (2) invincibility and superiority of COVID hoaxers, (3) conspiracies and/or hidden agendas. The findings of the study show that among all gender groups, the first category is the most dominant (44.4%), the third category is the second most frequent (35.6%), and the last category (19.9%) is the least frequent. However, when the discussion is centered on men (40.2%) and gender and sexual minorities (GSM; 69.6%) groups, the last category is the most dominant with regard to stigmatizing GSM groups by falsely associating them with progressive secret agendas. As for women's group, being sympathetic to human rights and the perceived injustice against them during the pandemic constitute the most dominant category (51.5%). We discuss the implications of the study in the conclusion.

The gendered dimensions of the anti-mask and anti-lockdown movement on social media.

This paper examines the anti-mask and anti-lockdown online movement in connection to the COVID-19 pandemic. To combat the spread of the coronavirus, health officials around the world urged and/or mandated citizens to wear facemasks and adopt physical distancing measures. These health policies and guidelines have become highly politicized in some parts of the world, often discussed in association with freedom of choice and independence. We downloaded references to the anti-mask and anti-lockdown social media posts using 24 search terms. From a total of 4209 social media posts, the researchers manually filtered the explicit visual and textual content that is related to discussions of different genders. We used multimodal discourse analysis (MDM) which analyzes diverse modes of communicative texts and images and focuses on appeals to emotions and reasoning. Using the MDM approach, we analysed posts taken from Facebook and Instagram from active anti-mask and anti-lockdown users, and we identified three main discourses around the gendered discussion of the anti-mask movement including hypermasculine, sexist and pejorative portrayals of "Karen", and appropriating freedom and feminism discourses. A better understanding of how social media users evoke gendered discourses to spread anti-mask and anti-lockdown messages can help researchers identify differing reactions toward pandemic measures.

When Science Becomes Embroiled in Conflict: Recognizing the Public's Need for Debate while Combating Conspiracies and Misinformation.

Most democracies seek input from scientists to inform policies. This can put scientists in a position of intense scrutiny. Here we focus on situations in which scientific evidence conflicts with people's worldviews, preferences, or vested interests. These conflicts frequently play out through systematic dissemination of disinformation or the spreading of conspiracy theories, which may undermine the public's trust in the work of scientists, muddy the waters of what constitutes truth, and may prevent policy from being informed by the best available evidence. However, there are also instances in which public opposition arises from legitimate value judgments and lived experiences. In this article, we analyze the differences between politically-motivated science denial on the one hand, and justifiable public opposition on the other. We conclude with a set of recommendations on tackling misinformation and understanding the public's lived experiences to preserve legitimate democratic debate of policy.

Investigation of COVID-19 Misinformation in Arabic on Twitter: Content Analysis.

Background: The COVID-19 pandemic has been occurring concurrently with an infodemic of misinformation about the virus. Spreading primarily on social media, there has been a significant academic effort to understand the English side of this infodemic. However, much less attention has been paid to the Arabic side. Objective: There is an urgent need to examine the scale of Arabic COVID-19 disinformation. This study empirically examines how Arabic speakers use specific hashtags on Twitter to express antivaccine and antipandemic views to uncover trends in their social media usage. By exploring this topic, we aim to fill a gap in the literature that can help understand conspiracies in Arabic around COVID-19. Methods: This study used content analysis to understand how 13 popular Arabic hashtags were used in antivaccine communities. We used Twitter Academic API v2 to search for the hashtags from the beginning of August 1, 2006, until October 10, 2021. After downloading a large data set from Twitter, we identified major categories or topics in the sample data set using emergent coding. Emergent coding was chosen because of its ability to inductively identify the themes that repeatedly emerged from the data set. Then, after revising the coding scheme, we coded the rest of the tweets and examined the results. In the second attempt and with a modified codebook, an acceptable intercoder agreement was reached (Krippendorff α≥.774). Results: In total, we found 476,048 tweets, mostly posted in 2021. First, the topic of infringing on civil liberties (n=483, 41.1%) covers ways that governments have allegedly infringed on civil liberties during the pandemic and unfair restrictions that have been imposed on unvaccinated individuals. Users here focus on topics concerning their civil liberties and freedoms, claiming that governments violated such rights following the pandemic. Notably, users denounce government efforts to force them to take any of the COVID-19 vaccines for different reasons. This was followed by vaccine-related conspiracies (n=476, 40.5%), including a Deep State dictating pandemic policies, mistrusting vaccine efficacy, and discussing unproven treatments. Although users tweeted about a range of different conspiracy theories, mistrusting the vaccine's efficacy, false or exaggerated claims about vaccine risks and vaccine-related diseases, and governments and pharmaceutical companies profiting from vaccines and intentionally risking the general public health appeared the most. Finally, calls for action (n=149, 12.6%) encourage individuals to participate in civil demonstrations. These calls range from protesting to encouraging other users to take action about the vaccine mandate. For each of these categories, we also attempted to trace the logic behind the different categories by exploring different types of conspiracy theories for each category. Conclusions: Based on our findings, we were able to identify 3 prominent topics that were prevalent amongst Arabic speakers on Twitter. These categories focused on violations of civil liberties by governments, conspiracy theories about the vaccines, and calls for action. Our findings also highlight the need for more research to better understand the impact of COVID-19 disinformation on the Arab world.

Sex Workers' Lived Experiences With COVID-19 on Social Media: Content Analysis of Twitter Posts.

BACKGROUND: The COVID-19 pandemic has drawn attention to various inequalities in global societies, highlighting discrepancies in terms of safety, accessibility, and overall health. In particular, sex workers are disproportionately at risk due to the nature of their work and the social stigma that comes alongside it. OBJECTIVE: This study examines how public social media can be used as a tool of professional and personal expression by sex workers during the COVID-19 pandemic. We aimed to explore an underresearched topic by focusing on sex workers' experiences with the ongoing COVID-19 pandemic on the social media platform Twitter. In particular, we aimed to find the main issues that sex workers discuss on social media in relation to the COVID-19 pandemic. METHODS: A literature review followed by a qualitative analysis of 1458 (re)tweets from 22 sex worker Twitter accounts was used for this study. The tweets were qualitatively coded by theme through the use of intercoder reliability. Empirical, experimental, and observational studies were included in this review to provide context and support for our findings. RESULTS: In total, 5 major categories were identified as a result of the content analysis used for this study: concerns (n=542, 37.2%), solicitation (n=336, 23.0%), herd mentality (n=231, 15.8%), humor (n=190, 13.0%), and blame (n=146, 10.0%). The concerns category was the most prominent category, which could be due to its multifaceted nature of including individual concerns, health issues, concerns for essential workers and businesses, as well as concerns about inequalities or intersectionality. When using gender as a control factor, the majority of the results were not noteworthy, save for the blame category, in which sexual and gender minorities (SGMs) were more likely to post content. CONCLUSIONS: Though there has been an increase in the literature related to the experiences of sex workers, this paper recommends that future studies could benefit from further examining these 5 major categories through mixed methods research. Examining this phenomenon could recognize the challenges unique to this working community during the COVID-19 pandemic and potentially reduce the widespread stigma associated with sex work in general.

Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis.

Secreted fungal peptides are known to influence the interactions between the pathogen and host innate immunity. The aim of this study is to screen and evaluate secreted peptides from the fungus Rhizopus arrhizus var. delemar for their immunomodulatory activity. By using mass spectrometry and immuno-informatics analysis, we identified three secreted peptides CesT (S16), Colicin (S17), and Ca2+/calmodulin-dependent protein kinase/ligand (CAMK/CAMKL; S27). Culturing peripheral blood-derived monocytic macrophages (PBMMs) in the presence of S16 or S17 caused cell clumping, while culturing them with S27 resulted in the formation of spindle-shaped cells. S27-treated PBMMs showed cell cycle arrest at G0 phase and exhibited alternatively activated macrophage phenotype with pronounced reduction in scavenger receptors CD163 and CD206. Homology prediction indicated that IL-4/IL-13 is the immunomodulatory target of S27. Confirming this prediction, S27 initiated macrophage activation through phosphorylation of STAT-6; STAT-6 inhibition reversed the activity of S27 and reduced the formation of spindle-shaped PBMMs. Lastly, S27 treatment of PBMMs was associated with altered expression of key iron regulatory genes including hepcidin, ferroportin, transferrin receptor 1, and ferritin in a pattern consistent with increased cellular iron release; a condition known to enhance Rhizopus infection. Collectively, R. arrhizus var. delemar secretes peptides with immunomodulatory activities that support fungal pathogenesis. Targeting the IL-4/IL-13R/STAT-6 axis is a potential therapeutic approach to enhance the PBMM-mediated fungal phagocytosis. This represents a potential new approach to overcome lethal mucormycosis.

News loopholing: Telegram news as portable alternative media.

This paper deals with foreign state-run media outlets that disseminate Persian language news targeted to the Iranian public. More specifically, it focuses on the mobile news app Telegram by undertaking a content analysis of a sample of the top 400 most viewed stories across four channels, i.e., BBC Persian, Voice of America's Persian language service VOA Farsi, Radio Farda, and Iran International television channel. It also offers a topic modelling of all news stories posted. Results show that most of the news coverage centered on politics, particularly with an emphasis on internal Iranian issues, while a few other channels repeatedly urged their followers to submit not only their email addresses and other private information, but also photographs and/or videos of anti-government protests. Conceptually, I consider these channels as portable alternative media, as opposed to state-run news media, since the Iranian public seeks them out as sources of political information that assist them in better understanding world news and, most importantly, news about their own country. The Telegram instant messaging app is related to the meso dimension of alternative media, meaning that it is characterized by the unique production and dissemination means it utilizes. This paper concludes by highlighting the implications of foreign state-run news outlets using news loopholing to disseminate information, while simultaneously collecting private information about their users and/or potentially risking their safety.

The convergence of social media and other communication technologies in the promotion of illicit and controlled drugs.

Some social media platforms have strict regulations regarding the promotion of illicit and controlled drug on their sites. This study attempts to examine whether social media outlets like Twitter, Flickr and Tumblr have implemented practical measures to stop the active promotion of such drugs. We examined over 2.6 million social media posts taken from these three platforms. By focusing on keyword searches around mobile apps and communication means, we found evidence of ongoing opioid drug promotion, especially on Twitter followed by Flickr and Tumblr; we discuss our approach which effectively identifies posts related to the promotion of opioids and controlled drugs.

A thematic analysis of Instagram's gendered memes on COVID-19.

We investigate the gendered use of Instagram memes on COVID-19 using a mixed-analysis approach. We find that memes referencing women are mostly related to community support and healthcare, which often express gratitude for frontline workers, while the majority of memes on men refer to news and promotion as well as suffering due to the high death rates and other financial hardships. As for sexual and gender minorities, memes mostly mention community support similar to the case of the memes referencing women. We argue that internet memes offer insight into ongoing trends in the public's perceptions of pandemics, and they should be further examined because they often communicate vital information on gender groups and public health.

Investigating Public Discourses Around Gender and COVID-19: a Social Media Analysis of Twitter Data.

We collected over 50 million tweets referencing COVID-19 to understand the public's gendered discourses and concerns during the pandemic. We filtered the tweets based on English language and among three gender categories: men, women, and sexual and gender minorities. We used a mixed-method approach that included topic modelling, sentiment analysis, and text mining extraction procedures including words' mapping, proximity plots, top hashtags and mentions, and most retweeted posts. Our findings show stark differences among the different genders. In relation to women, we found a salient discussion on the risks of domestic violence due to the lockdown especially towards women and girls, while emphasizing financial challenges. The public discourses around SGM mostly revolved around blood donation concerns, which is a reminder of the discrimination against some of these communities during the early days of the HIV/AIDS epidemic. Finally, the discourses around men were focused on the high death rates and the sentiment analysis results showed more negative tweets than among the other genders. The study concludes that Twitter influencers can drive major online discussions which can be useful in addressing communication needs during pandemics.

BL-8040 CXCR4 antagonist is safe and demonstrates antileukemic activity in combination with cytarabine for the treatment of relapsed/refractory acute myelogenous leukemia: An open-label safety and efficacy phase 2a study.

BACKGROUND: CXCR4 mediates the retention and survival of acute myelogenous leukemia blasts in bone marrow and contributes to their resistance to chemotherapy. The authors evaluated a combination of the high-affinity CXCR4 antagonist BL-8040 with high-dose cytarabine (HiDAC) chemotherapy in a phase 2a study of patients with relapsed and refractory AML. METHODS: Forty-two patients received treatment with BL-8040 monotherapy for 2 days followed by a combination of BL-8040 with HiDAC for 5 days. Six escalating BL-8040 dose levels were investigated (0.5, 0.75, 1.0, 1.25, 1.5, and 2.0 mg/kg), and 1.5 mg/kg was selected as the dose for the expansion phase (n = 23). RESULTS: BL-8040 in combination with HiDAC was safe and well tolerated at all dose levels. Clinical response was observed with BL-8040 doses ≥1.0 mg/kg. The composite response rate (complete remissions plus complete remissions with incomplete hematologic recovery of platelets or neutrophils) was 29% (12 of 42) in all patients and 39% (9 of 23) in the 1.5-mg/kg phase. The median overall survival was 8.4 months for all patients, 10.8 months in the 1.5-mg/kg phase, and 21.8 months for responding patients in the 1.5-mg/kg cohort. Two days of BL-8040 monotherapy triggered the mobilization of blasts into peripheral blood, with significantly higher mean fold-changes in responders versus nonresponders. This was accompanied by a decrease in bone marrow blasts. CONCLUSIONS: The current results demonstrate the efficacy of CXCR4 targeting with BL-8040 and support continued clinical development in acute myelogenous leukemia.

COVID-19 and the Gendered Use of Emojis on Twitter: Infodemiology Study.

BACKGROUND: The online discussion around the COVID-19 pandemic is multifaceted, and it is important to examine the different ways by which online users express themselves. Since emojis are used as effective vehicles to convey ideas and sentiments, they can offer important insight into the public's gendered discourses about the pandemic. OBJECTIVE: This study aims at exploring how people of different genders (eg, men, women, and sex and gender minorities) are discussed in relation to COVID-19 through the study of Twitter emojis. METHODS: We collected over 50 million tweets referencing the hashtags #Covid-19 and #Covid19 for a period of more than 2 months in early 2020. Using a mixed method, we extracted three data sets containing tweets that reference men, women, and sexual and gender minorities, and we then analyzed emoji use along each gender category. We identified five major themes in our analysis including morbidity fears, health concerns, employment and financial issues, praise for frontline workers, and unique gendered emoji use. The top 600 emojis were manually classified based on their sentiment, indicating how positive, negative, or neutral each emoji is and studying their use frequencies. RESULTS: The findings indicate that the majority of emojis are overwhelmingly positive in nature along the different genders, but sexual and gender minorities, and to a lesser extent women, are discussed more negatively than men. There were also many differences alongside discourses of men, women, and gender minorities when certain topics were discussed, such as death, financial and employment matters, gratitude, and health care, and several unique gendered emojis were used to express specific issues like community support. CONCLUSIONS: Emoji research can shed light on the gendered impacts of COVID-19, offering researchers an important source of information on health crises as they happen in real time.