Baseline distribution of petroleum hydrocarbon contamination in the marine environment around the coastline of Qatar.

Levels of organic contaminants (TPHs, PAHs) were simultaneously determined in both abiotic (sediments, seawater) and biotic (Pinctada radiata oysters) samples at four sites along the coastline of Qatar (Arabian Gulf) in 2017-2018. TPHs and PAHs were more frequently detected in oyster tissues than sediment and seawater samples collected from the same areas. While levels of TPHs and PAHs in seawater and sediments were lower than previous local studies and worldwide studies, PAHs levels observed in pearl oyster tissue (25.9-2240 µg/kg) were relatively higher than in previous studies in Qatar. In general, eight PAHs compounds were detected in oyster tissue, with benzo(a)pyrene displaying the highest concentration. The coast of Qatar could be affected by seasonal patterns of pollutants, where TPHs and PAHs levels increased in winter compared to summer. These results provide key information on the use of the pearl oyster as a bioindicator species and Qatar's marine environment.

Biocontrol Activity of Bacillus megaterium BM344-1 against Toxigenic Fungi.

Mycotoxins are secondary metabolites of some fungal species and represent important contaminants of food and feed. This study aimed to explore the biological control activity of Bacillus megaterium BM344-1 volatile organic compounds (VOCs) on the growth and mycotoxin production of single representatives of the toxigenic species Aspergillus flavus, Aspergillus carbonarius, Penicillium verrucosum, and Fusarium verticillioides. In vitro co-incubation experiments indicated the P. verrucosum isolate as the most sensitive one, with a growth inhibition ratio of 66.7%, followed by A. flavus (29.4%) and F. verticillioides (18.2%). Exposure of A. flavus, P. verrucosum, and F. verticillioides to BM344-1 VOCs resulted in complete inhibition of aflatoxins (AFB1, AFG1, and AFG2), ochratoxin A, and fumonisin B1 (FB1) synthesis on artificial media, respectively. In vivo experiments on maize kernels showed 51% inhibition of fungal growth on ears simultaneously infected with A. flavus spores and exposed to BM344-1 volatiles. Likewise, AF synthesis by A. flavus was significantly (p < 0.05) inhibited (25.34 ± 6.72 µg/kg) by bacterial volatiles as compared to that in control maize ears (91.81 ± 29.10 µg/kg). Gas chromatography-tandem mass spectrometry-based analysis of headspace volatiles revealed hexadecanoic acid methyl ester (palmitic acid) and tetracosane as bioactive compounds in the BM344-1 volatilome. Bacterial volatiles have promising potential to control the growth and mycotoxin synthesis of toxigenic fungi and may present valuable aid in the efforts to warrant food and feed safety.

Isolation of a Novel Kluyveromyces marxianus Strain QKM-4 and Evidence of Its Volatilome Production and Binding Potentialities in the Biocontrol of Toxigenic Fungi and Their Mycotoxins.

To overcome the economic losses associated with fungi and their toxic metabolites, environmentally safe and efficient approaches are needed. To this end, biological control using yeasts and safe bacterial strains and their products are being explored to replace synthetic fungicides. In the present study, the biocontrol effect of a yeast strain of Kluyveromyces marxianus, QKM-4, against the growth and mycotoxin synthesis potential of key toxigenic fungi was evaluated. In vitro assays were performed to find the application of yeast volatile organic compounds (VOCs) against fungal contamination on important agricultural commodities. The removal of ochratoxin A (OTA) and deoxynivalenol (DON) by living and heat-inactivated yeast cells was also explored. VOCs produced by strain QKM-4 were able to significantly limit the fungal growth of 17 fungal species belonging to genera Aspergillus, Penicillium, and Fusarium. Yeast VOCs were able to reduce OTA biosynthesis potential of Penicillium verrucosum and Aspergillus carbonarius by 99.6 and 98.7%, respectively. In vivo application of QKM-4 VOCs against Fusarium oxysporum and A. carbonarius infection on tomatoes and grapes, respectively, determined a complete inhibition of fungal spore germination. GC/MS-based analysis of yeast VOCs identified long-chain alkanes, including nonadecane, eicosane, docosane, heptacosane, hexatriacontane, and tetracosane. In vitro testing of the mycotoxin-binding potential of the living and heat-inactivated QKM-4 cells showed a reduction of OTA and DON up to 58 and 49%, respectively, from artificially contaminated buffers. Our findings clearly demonstrate the strong antifungal potential of K. marxianus QKM-4 and propose this strain as a strong candidate for application in agriculture to safeguard food and feed products.

Characteristics and sources of anthropogenic and biogenic hydrocarbons in sediments from the coast of Qatar.

Surface sediment samples from the coastal zone of Qatar were collected and analyzed to determine the characteristics, and sources of anthropogenic and biogenic hydrocarbons. The main compounds in these surface sediments included n-alkanes, methyl n-alkanoates, diterpenoids, hopanes, steranes, phthalate esters, polycyclic aromatic hydrocarbons (PAHs) and unresolved complex mixture (UCM). Their total concentrations ranged from 18.7±3.7-81.1±7.5ng/g (3.7±0.6-10.4±4.8%) for n-alkanes, 8.3±2.3-51±3.4ng/g (3.0±2.0-5.6±2.0%) for methyl n-alkanoates, 1.8±0.1-10.5±1.0ng/g (1.0±0.5-0.4±0.1%) for diterpenoids, 0.0-79.3±7.4ng/g (0.0-7.9±0.6%) for hopanes, 0.0-32.9±7.9ng/g (0.0-6.5±1.0%) for steranes, 0.7±0.1-36.3±3.4ng/g (0.1±0.1-1.9±3.4%) for phthalates, 0.30±0.2-7.8±0.7ng/g (0.02±0.04-0.42±0.72%) for PAHs, and 38±9-609±57ng/g (38.5±13.4-56.5±13.4%) for UCM. The major sources of these lipids were anthropogenic petroleum residues and plasticizers (80-89%), with lesser amounts from natural higher plants and microbial residues (11-20%). Petroleum residues and plasticizer inputs to the coastal sediments of Qatar likely affect the marine ecosystems and associated species groups as well as shallow coastal nursery and spawning areas.

Evaluation of pesticide residues of organochlorine in vegetables and fruits in Qatar: statistical analysis.

The study aimed to examine the residues of organochlorines pesticides (OCPs) in vegetables and fruits in Qatar. A total of 127 samples was studied. Ninety percent of the imported samples recorded residues above the maximum residue levels (MRLs). The most frequently detected OCP in the samples was heptachlor (found in 75 samples). In the comparisons between the washed and unwashed samples, no significant differences were observed (P > 0.05). However, the effect of washing process with tap water depended on the type of vegetables and fruits.