RESUMO
The aim of the research was to determine the impact of fermentation with Pleurotus ostreatus on kidney beans, black beans, and oats. The results indicate that the fungus has a positive effect on the substrates when compared to the controls. The antioxidant activity (39.5% on kidney beans and 225% on oats in relation to the controls) and content of total polyphenols (kidney beans three times higher regarding the controls) increased significantly by the presence of the fungus mycelium, even after simulated digestion. There was a significant increase in protein digestibility (from 39.99 to 48.13% in black beans, 44.06 to 69.01% in kidney beans, and 63.25 to 70.01% in oats) and a decrease of antinutrient tannins (from 65.21 to 22.07 mg in black beans, 35.54 to 23.37 in kidney beans, and 55.67 to 28.11 in oats) as well as an increase in the contents of some essential amino acids. Overall, this fermentation treatment with Pleurotus ostreatus improved the nutritional quality of cereals and legumes, making them potential ingredients for the elaboration and/or fortification of foods for human nutrition.
Assuntos
Antioxidantes/química , Avena/química , Proteínas Alimentares/química , Phaseolus/química , Pleurotus/metabolismo , Aminoácidos/química , Aminoácidos/metabolismo , Antioxidantes/metabolismo , Avena/metabolismo , Carboidratos/química , Proteínas Alimentares/metabolismo , Fermentação , Humanos , Micélio/metabolismo , Valor Nutritivo , Phaseolus/metabolismo , Polifenóis/química , Polifenóis/metabolismo , Sementes/química , Sementes/metabolismo , Taninos/química , Taninos/metabolismoRESUMO
We describe a novel bacteriocin screening assay based on fluorescence emitted by berberine following its influx into compromised cells. This technique showed agreement with the conventional well-diffusion method, and results can be obtained within one hour. This assay could facilitate the rapid identification of bacteriocinogenic bacterial isolates.
