Analysis of Muscle Oxygenation after a Normobaric Hypoxia Tolerance Test.

The aim of this work was to analyze the influence of acute normobaric hypoxia on quadricep oxygenation. Muscle oxygen saturation (SmO2) was measured using near-infrared spectrometry (NIRS) technology during a normobaric hypoxia tolerance test (NHTT). SmO2 was measured with a Humon Hex® device. In total, 54 healthy subjects participated, 68.5 of which were males and 31.5% of which were females. They performed an NHTT with the IAltitude® simulator, breathing air with an FiO2 level of 11% (equivalent to 5050 m). The maximum duration of the NHTT was set at 10 min, stopping if it reached 83% SpO2. The initial values (PRE) were compared with those obtained at the end of the test (POST) and after 10 min of recovery. The participants were divided into two groups based on whether (G1) they completed the ten minutes or not (G2). In total, 35.1% of men and 41.2% of women completed the 10 min. In both groups, significant differences were observed in the decrease in SmO2 values (p < 0.0001) (G1: PRE = 59.5 ± 12.48%; POST = 55.95 ± 14.30%; G2: PRE = 60.06 ± 13.46%; POST = 57.2 ± 12.3%). There were no differences between groups in any of the three periods. Exposure to normobaric hypoxia produces a decrease in quadricep levels of SmO2 in both sexes, regardless of whether the test is completed. Two patterns appeared: A.-less time and more hypoxia; B. a longer duration and less hypoxia.

Ventilatory Pattern Influences Tolerance to Normobaric Hypoxia in Healthy Adults.

INTRODUCTION: Tolerance to breathing in conditions with a decreased oxygen ratio is subject-specific. A normobaric hypoxia tolerance test (NHTT) is performed to assess the ability of each individual, as this may be influenced by genetic or personal factors such as age or gender. The aim of this study is to test the influence of deep breathing on hypoxia tolerance time. MATERIAL AND METHODS: A total of 45 subjects (21 parachutists and 24 students) performed two NHTTs at 5050 m altitude (iAltitude). Arterial (SatO2) and muscle (SmO2) oxygen saturation were monitored with the Humon Hex® device. The first NHTT was performed with free breathing, without any instructions; and the second NHTT was performed with wide, slow, diaphragmatic breathing. The NHTT was terminated at the end of 10 min or when a value of less than 83% was obtained. RESULTS: The first NHTT was completed by 38.1% of parachutist and 33.3% of students while the second NHTT was completed by 85.7% and 75%, respectively. In the second NHTT, both parachutists and students had a significantly (p = 0.001) longer duration compared to the first NHTT. SmO2 and SatO2 values also increased significantly (p < 0.001) in both groups (p < 0.05). CONCLUSION: Performing controlled diaphragmatic breathing is successful in increasing hypoxia tolerance time and/or SatO2 values.

Influences of Intense Physical Effort on the Activity of the Autonomous Nervous System and Stress, as Measured with Photoplethysmography.

Background: The autonomic nervous system, which is composed of the sympathetic and parasympathetic nervous system, is closely related to the cardiovascular system. The temporal variation between each of the intervals between the consecutive "R" waves of an electrocardiogram is known as heart rate variability. Depending on the type of activity, both systems can be activated, and also influence the interval between "R" waves. Currently, with advancements in technology and electronic devices, photoplethysmography is used. Photoplethysmography detects changes in the intensity of reflected light that allow differentiation between systole and diastole and, therefore, determines the heart rate, its frequency and its variations. In this way, changes in the autonomic nervous system can be detected by devices such as the Max Pulse®. Objective: To determine whether the information provided by Max Pulse® on autonomic balance and stress is modified after intense physical exercise, thereby determining whether there is a relationship with body composition, and also whether there are differences with respect to gender. Materials and Methods: Fifty-three runners (38.9% female) with a mean age of 31.3 ± 8.1 years participated in the study. Two measurements (before and after intense physical effort) were performed with the Max Pulse® device. The flotoplethysmography measurement lasted 3 min, and was performed in the supine position. The exercise test was performed on a treadmill. It was initiated at a speed of 6 and 7 km/h for women and men, respectively. Subjects indicated the end of the test by making a hand gesture when unable to continue the test. Results: Autonomic nervous system activity and mental stress values decreased significantly (p < 0.05) in men and women, while autonomic nervous system balance decreased only in women. Physical stress increased (p < 0.05) in both sexes. Conclusions: Intense exercise causes changes in variables that assess autonomic nervous system balance and stress, as measured by a device based on photoplethysmography. The changes are evident in both sexes, and are not related to body composition.

Estudio de la variabilidad de la frecuencia cardiaca tras la exposición a la hipoxia normobárica / Study of heart rate variability after exposure to normobaric hypoxia

La variabilidad de la frecuencia cardiaca (VFC) es una herramienta capaz de analizar y valorar la actividad vegetativa sobre el corazón ante diversas actividades y situaciones. Consiste en medir el tiempo que trascurre entre cada dos latidos cardiacos durante un periodo de tiempo y expresarlo en función de ecuaciones matemáticas y estadísticas. Otros autores han analizado la influencia de diferentes estresores sobre la VFC. En este trabajo buscamos la acción de la hipoxia normobárica (HN) sobre la misma. La HN consiste en respirar aire empobrecido de oxígeno simulando el entrenamiento en altitud. El objetivo del estudio es determinar la influencia de la HN sobre los dominios de tiempo y frecuencia de la VFC. Sometimos a 13 sujetos sanos (deportistas recreacionales) a dos sesiones de HN. Usamos el simulador iAltitude Trainer v2.7®. La primera mediante un test de tolerancia a la hipoxia (TTH) (10 minutos, 11% O2, equivalente a 5.050 m) y, la segunda, con una exposición intermitente (HNI) (14% O2, 3.250 m) en la que se alternaron periodos de 4 minutos de hipoxia con 4 de normoxia durante 64 minutos. Para el análisis de VFC se utilizó un pulsómetro Polar H10®, la aplicación HRV-elite® y el software Kubios-Standard®. Se tomaron los datos de los 5 minutos previos y posteriores a cada sesión, comparándose estos valores mediante el test de T-student para datos pareados. Ninguna de las variables de los dominios de tiempo (RRmedio, SDNN, rMSSD, pNN50) ni de frecuencia (VLF, LF, HF, LF/HF) de la VFC mostró cambios significativos ante ninguna de las dos situaciones. La HN no provocó modificaciones en los niveles de estrés de estos sujetos, siendo bien tolerada, clínica y electrocardiográficamente. Un test de tolerancia y una sesión de exposición a hipoxia normobárica intermitente no son estímulos suficientes para provocar cambios agudos en la VFC

Heart rate variability (HRV) is a tool capable of analysing and assessing the vegetative activity of the heart in various activities and situations. It consists of measuring the time that elapses between every two heartbeats over a period of time and expressing it in terms of mathematical and statistical equations. Other authors have analysed the influence of different stressors on HRV. In this work we are looking for the action of normobaric hypoxia (NH) on HRV. NH consists of breathing oxygen-depleted air simulating altitude training. The aim of the study is to determine the influence of HN on the time and frequency domains of HRV. We subjected 13 healthy subjects (recreational athletes) to two HN sessions. We used the iAltitude Trainer v2.7® simulator. The first was a hypoxia tolerance test (HTT) (10 minutes, 11% O2, equivalent to 5050m) and the second was an intermittent exposure (HNI) (14% O2, 3250m) in which periods of 4 minutes of hypoxia alternated with 4 minutes of normoxia for 64 minutes. For HRV analysis, a Polar H10® heart rate monitor, the HRV-elite® application and the Kubios-Standard® software were used. Data were taken 5 minutes before and after each session, and these values were compared using the Student ́s t- test for paired data. None of the variables in the time (RRmean, SDNN, rMSSD, pNN50) or frequency (VLF, LF, HF, LF/HF) domains of HRV showed significant changes in either situation. HN did not cause changes in the stress levels of these subjects and was well tolerated, clinically and electrocardiographically. A tolerance test and a session of exposure to intermittent normobaric hypoxia are not sufficient stimuli to cause acute changes in HRV.(AU)

The Effect of Normobaric Hypoxia in Middle- and/or Long-Distance Runners: Systematic Review.

BACKGROUND: The use of normobaric hypoxia can bring benefits to sports performance because it improves haematological parameters and/or physical activity tests. Our objective was to conduct a systematic review so as to analyse the methods used in hypoxia and to detect its effects on middle- and/or long-distance runners. METHODS: Research was conducted using five electronic databases (PubMed, SportDiscus, Cochrane Library, Scopus and PEDro) until December 2021. The methodological quality of the included studies was assessed using the PEDro scale. RESULTS: Having analysed 158 studies, 12 were chosen for the qualitative and quantitative synthesis. A significant improvement on time until exhaustion was detected, and oxygen saturation decreased after the intervention. There were no significant changes in the 3000-metre time trial or in the haematocrit percentage. The changes in percentage of reticulocytes, heart rate, maximal heart rate, lactate concentration and erythropoietin were heterogeneous between the different research studies. CONCLUSION: short exposure (less than 3 h to normobaric hypoxia significantly increases the time to exhaustion). However, longer exposure times are necessary to increase haemoglobin. Altitude and exposure time are highly heterogeneous in the included studies.