Erratum: The shape of lipsmacking: socio-emotional regulation in bearded capuchin monkeys (Sapajus libidinosus) - CORRIGENDUM.

[This corrects the article DOI: 10.1017/ehs.2023.10.].

The shape of lipsmacking: socio-emotional regulation in bearded capuchin monkeys (Sapajus libidinosus).

Capuchin monkeys have rich social relationships and from very young ages they participate in complex interactions with members of their group. Lipsmacking behaviour, which involves at least two individuals in socially mediated interactions, may tell about processes that maintain, accentuate or attenuate emotional exchanges in monkeys. Lipsmacking is a facial expression associated with the establishment and maintenance of affiliative interactions, following under the 'emotional regulation' umbrella, which accounts for the ability to manage behavioural responses. We investigated behaviours related to the emitter and to the receiver (infant) of lipsmacking to answer the question of how lipsmacking occurs. In capuchin monkeys, lipsmacking has been previously understood solely as a face-to-face interaction. Our data show that emitters are engaged with infants, looking longer towards their face and seeking eye contact during the display. However, receivers spend most of the time looking away from the emitter and stay in no contact for nearly half of the time. From naturalistic observations of wild infant capuchin monkeys from Brazil we found that lipsmacking is not restricted to mutual gaze, meaning there are other mechanisms in place than previously known. Our results open paths to new insights about the evolution of socio-emotional displays in primates.

Dogs functionally respond to and use emotional information from human expressions.

Emotions are critical for humans, not only feeling and expressing them, but also reading the emotional expressions of others. For a long time, this ability was thought to be exclusive to people; however, there is now evidence that other animals also rely on emotion perception to guide their behaviour and to adjust their actions in such way as to guarantee success in their social groups. This is the case for domestic dogs, who have tremendously complex abilities to perceive the emotional expressions not only of their conspecifics but also of human beings. In this paper we discuss dogs' capacities to read human emotions. More than perception, though, are dogs able to use this emotional information in a functional way? Does reading emotional expressions allow them to live functional social lives? Dogs can respond functionally to emotional expressions and can use the emotional information they obtain from others during problem-solving, that is, acquiring information from faces and body postures allows them to make decisions. Here, we tackle questions related to the abilities of responding to and using emotional information from human expressions in a functional way and discuss how far dogs can go when reading our emotions.

How Do Dogs Behave When Presented with Situations of Different Emotional Valences?

Dogs are good models for studying behaviour and cognition as they have complex social capabilities. In the current study, we observed how human emotional valences (positive, neutral and negative) affected aspects of dogs' behaviour. We expected that dogs would exhibit more approaching behaviours in the positive condition and more signs of avoidance in the negative one. We analysed videos of 70 adult pet dogs of various breeds taken from an experiment in which one of two actors expressed an emotion and dogs could freely explore the environment for 30 s. Our results show that dogs exhibit differential behaviour when presented with different emotional valences. Two behaviours arose that might be linked to a reciprocal positive emotional state in dogs: tail raised between 90° and 180° and physical contact during sniffing. These behaviours are associated with an active search for information. In the positive conditions, dogs were more willing to explore the social environment and gather information from the actors.

The Use and Effectiveness of Ceftazidime-Avibactam in Real-World Clinical Practice: EZTEAM Study.

INTRODUCTION: Ceftazidime-avibactam has proven activity against multidrug-resistant (MDR) bacteria in clinical trials and real-world studies. This study was conducted to describe the patterns of use of ceftazidime-avibactam (including indications and associated antibiotics), and the effectiveness and safety of ceftazidime-avibactam in real-world clinical practice. METHODS: This non-interventional medical chart review study was conducted in 11 countries across the European and Latin American (LATAM) regions. Consecutive patients treated in clinical practice with at least one dose of ceftazidime-avibactam for an approved indication per country label since 01 January 2018 (or launch date in the country if posterior) were enrolled. Effectiveness analyses were conducted in patients treated with ceftazidime-avibactam for at least 72 h. RESULTS: Of the 569 eligible patients enrolled, 516 (90.7%) were treated for at least 72 h (354 patients from Europe and 162 patients from LATAM); 390 patients (75.7%) had switched from another antibiotic line for Gram-negative coverage. Infection sources were intra-abdominal, urinary, respiratory, bloodstream infections, and other infections (approximately 20% each). K. pneumoniae was the most common microorganism identified in the latest microbiological evaluation before starting ceftazidime-avibactam (59.3%). Two-thirds of microorganisms tested for susceptibility were MDR, of which 89.3% were carbapenem-resistant. The common MDR mechanisms for K. pneumoniae were carbapenemase (33.9%), oxacillinase 48 (25.2%), extended-spectrum beta-lactamase (21.5%), or metallo-beta-lactamase (14.2%) production. Without prior patient exposure, 17 isolates (mostly K. pneumoniae) were resistant to ceftazidime-avibactam. Treatment success was achieved in 77.3% of patients overall (88.3% among patients with urinary infection), regardless of first or second treatment line. In-hospital mortality rate was 23.1%. Adverse events were reported for six of the 569 patients enrolled. CONCLUSION: This study provides important real-world evidence on treatment patterns, effectiveness, and safety of ceftazidime-avibactam in clinical practice through its recruitment in the European and LATAM regions. Ceftazidime-avibactam is one of the antibiotics to consider for treatment of MDR bacteria. TRIAL REGISTRATION: ClinicalTrials.gov identifier, NCT03923426.

Use of wildlife as an alternative protein source: Collared peccary meat.

Knowledge on the nutritional and sensory characteristics of wild meat provides a better basis for its use as food, ultimately expanding studies in gastronomy areas and stimulating the development of new products. This review aims to present information on the use of wild meat, with a focus on collared peccaries (Pecari tajacu). The biological characteristics of collared peccaries are discussed, with a focus on the main aspects of their meat. Collared peccary meat has excellent nutritional value due to its high protein levels (18.25%), unsaturated fatty acids (51.6-57.8%), and tenderness, similar to other domestic animals, thereby stimulating the interest of a new product market. Despite the demand for this product, collared peccary meat is scarce and not readily available for commercialization. Further, public policies are needed to encourage the management of this species to add value to the development of a production chain.

Entrecruzamento de desigualdades: gênero e maternidade na carreira de médicas de família e comunidade / Intercrossing inequalities: gender and maternity in the career of family and community physicians

O estudo da crescente feminização da área da Medicina de Família e Comunidade coloca na agenda de estudiosos a preocupação com os fatores e impactos dessa tendência nas trajetórias das carreiras das médicas, principalmente daquelas que estão exercendo suasatividades em áreas periféricas. O objetivo do estudo do qual resulta este artigo foi compreender como as médicas justificam suas escolhas e permanências no exercício da Medicina de Família e Comunidade, mesmo em contextos considerados potencialmente violentos. Realizamos uma investigação de caráter qualitativo, que dialogou com aspectos da cartografia, a partir de entrevistas realizadas com oito médicas de família que se fixaram em áreas de alta vulnerabilidade social em uma capital brasileira. A análise dos enunciados apontou para o gênero como o eixo marcador de diferença, destacando o empuxo exercido pela maternidade, o conceito gênero em sua dinâmica interseccional. Identificamos que a responsabilização pelo cuidado permanece impactando o processo decisório das mulheres, (re)definindo o equilíbrio entre vida pessoal/familiar e trabalho e (de)limitando as trajetórias de suas carreiras.

The growing trend of feminization of family practice raises scholars' concerns about the factors and effects of this trend on the trajectories of female medical careers, particularly those working in outlying areas. The aim of the study, from which this article results, was to understand how medical women justify their choices and permanence in the family practice career while working in contexts considered potentially violent. We carried out a qualitative study with cartographic aspects, based on interviews with eightfamily doctors who are settled in areas of high social vulnerability in a large city in Brazil. The analysis of the statements pointed to gender, in its intersectional dynamics, as the major axis of difference in the practice. Motherhood is highlighted ashaving a major impact on female professionals. Our study identified that the duty of care persists in impacting women's decision-making, (re) defining the balance between personal and family life and (de) limiting their career trajectories.

Size, skull shape and age influence the temperament of domestic dogs.

Studies have investigated the relationship between dogs' morphological characteristics and behavioural dimensions, but little is discussed about this relationship in the context of dogs' responses to emotion eliciting stimuli and temperament. Based on the assumption that the developmental trajectory of behaviour and emotion processing is impacted by the interaction between organisms and environment, we analysed whether dogs' characteristics - that determine how they perceive and interact with their surroundings - affect temperament profiles. We looked at size, height, weight, skull morphology, age, sex, reproductive status, and breed of a sample of Brazilian domestic dogs' and their temperament (measured as Positive and Negative Activation), obtained via the PANAS questionnaire for dogs. We tested possible main effects and first-order interactions in linear mixed models. Younger dogs showed higher positive activation (i.e. sensitivity to positive stimuli; play and treats). For short snouted dogs, the older the dog, less was the negative activation (i.e. sensitivity to negative stimuli; punishment). And for long snouted dogs, larger dogs showed lower negative activation. Weight alone also showed an effect, with heavier dogs having less sensitivity to negative stimuli. Overall, our study demonstrated how morphology and age are important factors that influence temperament expression and emotional reactivity in dogs.

Dogs can infer implicit information from human emotional expressions.

The ability to infer emotional states and their wider consequences requires the establishment of relationships between the emotional display and subsequent actions. These abilities, together with the use of emotional information from others in social decision making, are cognitively demanding and require inferential skills that extend beyond the immediate perception of the current behaviour of another individual. They may include predictions of the significance of the emotional states being expressed. These abilities were previously believed to be exclusive to primates. In this study, we presented adult domestic dogs with a social interaction between two unfamiliar people, which could be positive, negative or neutral. After passively witnessing the actors engaging silently with each other and with the environment, dogs were given the opportunity to approach a food resource that varied in accessibility. We found that the available emotional information was more relevant than the motivation of the actors (i.e. giving something or receiving something) in predicting the dogs' responses. Thus, dogs were able to access implicit information from the actors' emotional states and appropriately use the affective information to make context-dependent decisions. The findings demonstrate that a non-human animal can actively acquire information from emotional expressions, infer some form of emotional state and use this functionally to make decisions.

Liderança feminina: relato do primeiro encontro de mulheres Médicas de Família e Comunidade do Brasil / Women leadership: report of the first meeting of Female Family Physicians in Brazil

RESUMO Este relato situa-se no campo do protagonismo das mulheres Médicas de Família e Comunidade (mMFC) e em sua articulação nacional por meio do Grupo de Trabalho Mulheres na Medicina de Família e Comunidade (GT-MMF), fundado em 2016 no bojo da Sociedade de Medicina de Família e Comunidade (SBMFC), entidade científica que representa a especialidade no País. Descreve a organização do I Encontro do GT-MMFC, em 2019, intitulado 'Liderança feminina em saúde' e discute seus desdobramentos, com foco na equidade de gênero nos domínios: profissional, acadêmico, de gestão, de ensino e pesquisa; assim como na própria instituição, a SBMFC. O artigo se debruça, ainda, sobre questões relacionadas com as causas de mulheres no âmbito da especialidade e da medicina. O evento foi aberto a estudantes e profissionais de outras áreas e ofertou discussões contemporâneas, como: protagonismo feminino; autocuidado; interseccionalidades; maternidade e trabalho; inserção da mulher e diferenças de gênero na política. O Encontro reuniu mulheres de quatro regiões do Brasil, aprofundou as relações e o apoio interpares e permitiu a ampliação das pautas para o fortalecimento da consciência de gênero e sua influência no cotidiano das mMFC, na sua prática acadêmica, científica, assistencial e de gestão.

ABSTRACT This report is situated in the scope of Female Family Physicians' protagonism and their national organization through the Women's Working Group on Family and Community Medicine, which was founded in 2016 under the Brazilian Society of Family and Community Medicine, a scientific entity that represents this medical specialty in the country. It describes the organization of the first Meeting of this Working Group, in 2019, named 'Female Leadership in Health' and discusses its unfoldings, focusing on gender equity in spheres such as: professional, academic, management, educational, and research, as well as permeating the institution itself. This article also focuses on women's issues surrounding both the specialty and medicine in general. The event welcomed students and professionals from other fields and offered contemporary debates, for example: female protagonism, self-care, intersectionality, maternity and work, participation of women in politics, and gender inequities. The Meeting gathered women from four regions of Brazil, deepened peer bonds and support, and enabled the expansion of the agenda of gender consciousness and its influence in women family physicians' daily life in their experience in management, university, science, and assistance.

Do Emotional Cues Influence the Performance of Domestic Dogs in an Observational Learning Task?

Using social information is not indiscriminate and being able to choose what to copy and from whom to copy is critical. Dogs are able to learn socially, to recognize, and respond to dog as well as human emotional expressions, and to make reputation-like inferences based on how people behave towards their owner. Yet, the mechanisms dogs use for obtaining and utilizing social information are still to be fully understood, especially concerning whether emotional cues influence dogs' social learning. Therefore, our main aim was to test the hypothesis that an emotionally charged (negative, positive, or neutral) interaction with the demonstrator of a "V" detour task prior to testing would affect subjects' performance, by: (i) changing the value of the information provided by the demonstrator or (ii) changing the valence of the learning environment. Our experimental design consisted of three phases: pre-test (subjects were allowed to solve the task alone); emotional display (dogs watched the unfamiliar human behaving in either a positive, negative or neutral way towards their owner); test (demonstrator showed the task and subjects were allowed to move freely). Only dogs that failed in pre-test were considered for analysis (n = 46). We analyzed four dependent variables: success, time to solve the task, latency to reach the fence and matching the side of demonstration. For each, we used four models (GEEs and GLMMs) to investigate the effect of (1) demographic factors; (2) experimental design factors (including emotional group); (3) behavior of the dog; and (4) side chosen and matching. All models took into account all trials (random effect included) and the first trials only. Our findings corroborate previous studies of social learning, but present no evidence to sustain our hypothesis. We discuss the possibility of our stimuli not being salient enough in a task that involves highly motivating food and relies on long and highly distracting interval between phases. Nevertheless, these results represent an important contribution to the study of dog behavior and social cognition and pave the way for further investigations.

Interação, ensino, saúde e comunidade pela perspectiva dos discentes de medicina / Interaction, teaching, health and community from the perspective of the medical student

O eixo educacional Interação, Ensino, Saúde e Comunidade tem como objetivo preparar o aluno de medicina para lidar com diferentes cenários profissionais. O estudo em questão pretende analisar, de acordo com a perspectiva do discente, a relevância das atividades desenvolvidas por esse eixo educacional na formação de médica. Assim, foram realizados 7 grupos focais, com graduandos do 2o ao 4o ano de faculdade do curso de medicina. Os primeiros seis grupos se concentraram em identificar o potencial e as limitações da disciplina e o último grupo em desenvolver sugestões para as limitações identificadas. A pesquisa revelou que há limitações a serem enfrentadas como a constante mudança de metodologias ativas adotadas para o eixo. Por outro lado, destacou-se a possibilidade de aplicar técnicas e desenvolver competências em diferentes cenários da prática de saúde, as quais promovem crescimento pessoal e profissional, ao mesmo tempo que fortalecem o ciclo de ensino-aprendizagem

Late-Relapsing Hepatitis after Yellow Fever.

One patient presented hyporexia, asthenia, adynamia, and jaundice two months after acute yellow fever (YF) onset; plus laboratory tests indicating hepatic cytolysis and a rebound of alanine and aspartate transaminases, and total and direct bilirubin levels. Laboratory tests discarded autoimmune hepatitis, inflammatory or metabolic liver disease, and new infections caused by hepatotropic agents. Anti-YFV IgM, IgG and neutralizing antibodies were detected in different times, but no viremia. A liver biopsy was collected three months after YF onset and tested positive for YFV antigens and wild-type YFV-RNA (364 RNA-copies/gram/liver). Transaminases and bilirubin levels remained elevated for five months, and the arresting of symptoms persisted for six months after the acute YF onset. Several serum chemokines, cytokines, and growth factors were measured. A similar immune response profile was observed in the earlier phases of the disease, followed by more pronounced changes in the later stages, when transaminases levels returned to normal. The results indicated viral persistence in the liver and continual liver cell damage three months after YF onset and reinforced the need for extended follow-ups of YF patients. Further studies to investigate the role of possible viral persistence and the immune response causing relapsing hepatitis following YF are also necessary.

Carta de Cuiabá - Mulheres, Médicas de Família e Comunidade, no Brasil: onde estamos e onde podemos chegar? / Cuiabá Letter - Women, Family and Community Physicians, in Brazil: where we are and where can we reach? / Carta de Cuiabá - Mujeres Médicas de Familia y Comunidad en Brasil: donde estamos y donde podemos llegar?

Este artigo é um breve relato histórico sobre a formação do Grupo de trabalho de Mulheres na Medicina de Família e Comunidade da SBMFC (GT-MMFC), que ocorreu em 2016. Em paralelo, descreve-se as principais ações do Wonca Working Party on Women & Family Medicine e do próprio GT-MMFC até os dias atuais. Os objetivos do artigo são registrar a construção deste grupo de trabalho assim como fomentar e fortalecer o debate de todas as dimensões relacionadas às mulheres e a medicina de família e comunidade e a equidade de gênero

This article is a historical report of the creation of the SBMFC Women's Working Group on Family and Community Medicine (GT-MMFC), which took place in 2016. In parallel, it describes, until the present day, the main actions of the Wonca Working Party Women & Family Medicine and of the GT-MMFC. The objectives of the article are to record the construction of this working group as well as to foster and strengthen the debate on all dimensions related to women and family and community medicine and gender equity.

Este artículo es un informe histórico de la creación del Grupo de Trabajo de Mujeres SBMFC sobre Medicina Familiar y Comunitaria (GT-MMFC), que tuvo lugar en 2016. Paralelamente, describe las principales acciones del Grupo de Trabajo de Mujeres y Medicina Familiar de Wonca y del GT-MMFC, hasta el día de hoy. Los objetivos del artículo son registrar la construcción de este grupo de trabajo, así como fomentar y fortalecer el debate sobre todas las dimensiones relacionadas con la mujer y la medicina familiar y comunitaria y la equidad de género

Assessment of emotional predisposition in dogs using PANAS (Positive and Negative Activation Scale) and associated relationships in a sample of dogs from Brazil.

The English version of the Positive and Negative Activation Scale (PANAS) is a useful tool for the assessment of dog temperament, helping to identify highly sensitive individuals that could be at risk of developing fears, phobias and anxiety problems, and potentially depressive states. This study evaluated the association between dogs' and owners' characteristics and dog temperament in Brazil. To accomplish this, we adapted and validated a Portuguese language version of PANAS for dogs. Data from 1744 owner-dog dyads were analysed and a two-factor structure similar to the original PANAS was revealed that met the requirements for validity and internal consistency. We found that dogs owned by women, neutered dogs and those who live in single-dog households show higher negative activation. Moreover, the older the owner, the less the negative activation for dogs that are bought. We also found that the older the dog, the less the positive activation, but this score is higher in dogs that sleep inside the house. Interestingly, mixed-breed dogs scored higher for both negative and positive emotional activation compared to purebreds. These findings alongside the particular profile of dogs in Brazil, including its large population of mixed-breed, emphasise the value of cross-cultural investigations in order to develop a full understanding of dog behaviour.

Goats prefer positive human emotional facial expressions.

Domestication has shaped the physiology and the behaviour of animals to better adapt to human environments. Therefore, human facial expressions may be highly informative for animals domesticated for working closely with people, such as dogs and horses. However, it is not known whether other animals, and particularly those domesticated primarily for production, such as goats, are capable of perceiving human emotional cues. In this study, we investigated whether goats can distinguish human facial expressions when simultaneously shown two images of an unfamiliar human with different emotional valences (positive/happy or negative/angry). Both images were vertically attached to a wall on one side of a test arena, 1.3 m apart, and goats were released from the opposite side of the arena (distance of 4.0 m) and were free to explore and interact with the stimuli during the trials. Each of four test trials lasted 30 s. Overall, we found that goats preferred to interact first with happy faces, meaning that they are sensitive to human facial emotional cues. Goats interacted first, more often and for longer duration with positive faces when they were positioned on the right side. However, no preference was found when the positive faces were placed on the left side. We show that animals domesticated for production can discriminate human facial expressions with different emotional valences and prefer to interact with positive ones. Therefore, the impact of domestication on animal cognitive abilities may be more far-reaching than previously assumed.

Mouth-licking by dogs as a response to emotional stimuli.

Dogs are able to perceptually discriminate emotional displays of conspecifics and heterospecifics and possess the cognitive prototypes for emotional categorisation, however, it remains unclear whether dogs can respond appropriately to this information. One way to assess associations between specific behaviours and the perception of emotionally competent stimuli is to look at other reliable measures that are related to cognitive and physiological processing. Using a cross-modal preferential looking paradigm (Albuquerque et al., 2016), we presented dogs with pairs of facial expressions (positive and negative) combined with an emotionally charged vocalisation (positive or negative) or a control sound (neutral) and coded their mouth-licking behaviour. We found an effect of the valence of the face image dogs were seeing on the onset of the mouth-licking, with higher frequencies of this behaviour in response to the negative faces compared to images with positive valence. However, neither the sound being played nor the interaction between image valence and sound affected the behaviour. We also found an effect of species with mouth-licking occurring more often towards human stimuli. This spontaneous differential behavioural response, combined with previous evidence of cognitive emotional processing in these animals, suggests that dogs may have a functional understanding of emotional expressions.

Metacercariae of Austrodiplostomum compactum (Lutz, 1928) (Trematoda, Diplostomidae) infecting the eyes of Plagioscion squamosissimus (Heckel, 1840) (Perciformes, Scienidae) from Lake Catalão, Amazonas, Brazil / Metacercárias de Austrodiplostomum compactum (Lutz, 1928) (Trematoda, Diplostomidae) infectando os olhos de Plagioscion squamosissimus (Heckel, 1840) (Perciformes, Scienidae) do Lago Catalão, Amazonas, Brasil

ABSTRACT Austrodiplostomum compactum is a digenetic trematode whith metacercariae that occur in the eyes of a wide variety of fish species. In Brazil, A. compactum metacercariae have been reported in many fish species, but there are only a few studies in the Amazon region. Due to the lack of information in the Amazon, the aim of this study was to report the occurrence of A. compactum metacercariae in the eyes of 15 specimens of Plagioscion squamosissimus from the Lake Catalão, in Amazonas, Brazil. Fishes were collected using gill nets placed randomly in the lake, euthanized in a field laboratory, and had their eyes dissected and examined under a stereomicroscope. A total of 801 metacercariae were registered infecting the eyes of the hosts with a parasitic prevalence of 100%. There was a significant positive correlation between the number of metacercariae recorded and the standard body length of fishes. No significant correlation was found between the number of metacercariae and the weight and relative condition factor (Kn) of the hosts. Values of the variance-to-mean ratio and Green's index suggested an aggregate distribution of the parasites in the hosts, with a low degree of aggregation. This is the first report of occurrence of A. compactum parasitizing a fish in the Lake Catalão.

RESUMO Austrodiplostomum compactum é um trematódeo digenético cuja metacercária ocorre nos olhos de uma grande variedade de espécies de peixes. No Brasil, metacercárias deste parasita foram relatadas em diversas espécies de peixes, mas existem poucos estudos na região amazônica. Devido à falta de informação na região amazônica, o objetivo deste estudo foi relatar a ocorrência de metacercárias de A. compactum nos olhos de 15 espécimes de Plagioscion squamosissimus provenientes do Lago Catalão, Amazonas, Brasil. Os peixes foram coletados usando redes de pesca distribuídas aleatoriamente no lago e sacrificados em laboratório de campo, onde os olhos foram removidos e examinados sob microscópio estereoscópico. Um total de 801 metacercárias foram registradas infectando os olhos do hospedeiro, com uma prevalência parasitária de 100%. Houve uma correlação positiva significativa entre o número de metacercárias e o comprimento padrão dos peixes. Nenhuma correlação significativa entre o número de metacercárias e o peso e o fator de condição relativa (Kn) dos hospedeiros foi evidenciada. Valores do índice de dispersão e do índice de Green sugerem uma distribuição agregada dos parasitas com um baixo grau de agregação nos hospedeiros. Este é o primeiro relato da ocorrência de A. compactum parasitando um peixe no Lago Catalão.

Sexual behavior of Pecari tajacu (Cetartiodactyla: Tayassuidae) during periovulatory and early gestation periods.

The goal of this study was to describe the sexual behavior in female and male collared peccary. Twenty females and twelve males were monitored in familiar and non-familiar units for two 60 days periods. During both phases, we recorded 2747 sexual interactions initiated by 20 different females toward males and 4461 sexual interactions initiated by 12 males toward females. The frequency of sexual interactions initiated per female significantly increased from proestrus to estrus, and they were significantly more frequently courted. Females initiated olfactory inspections 15.42 times more and were mounted 22.6 times more during estrus than during proestrus. Nulliparous and primiparous females copulated only when exposed to non-parental males. After estrus, the frequency of sexual interactions received by females sharply decreased. One mating event was recorded during the first gestation week and 31 mountings were observed after the second week. In conclusion, the behavioral monitoring is a useful procedure for the recognition of estrus. Our results suggest that ovulation may be associated with the end of the estrus, which will support future work in assisted reproduction in this species. To promote good handling practices, females of reproductive age should be removed from their family unit of origin.

Dogs recognize dog and human emotions.

The perception of emotional expressions allows animals to evaluate the social intentions and motivations of each other. This usually takes place within species; however, in the case of domestic dogs, it might be advantageous to recognize the emotions of humans as well as other dogs. In this sense, the combination of visual and auditory cues to categorize others' emotions facilitates the information processing and indicates high-level cognitive representations. Using a cross-modal preferential looking paradigm, we presented dogs with either human or dog faces with different emotional valences (happy/playful versus angry/aggressive) paired with a single vocalization from the same individual with either a positive or negative valence or Brownian noise. Dogs looked significantly longer at the face whose expression was congruent to the valence of vocalization, for both conspecifics and heterospecifics, an ability previously known only in humans. These results demonstrate that dogs can extract and integrate bimodal sensory emotional information, and discriminate between positive and negative emotions from both humans and dogs.