Evaluating toxic element contamination sources in groundwater bodies of two Mediterranean sites.

The Driver-Pressure-State-Impact-Response (DPSIR) framework is applied for assessing the pressures and impacts on groundwater bodies of two Mediterranean sites (Megara and Oropos-Kalamos basins). The study areas present joint driving forces (drivers) and pressures. The main driving forces in the areas studied mainly include geology, agricultural activities, and urban development, while the main pressures mainly include the weathering of ultramafic rock masses, application of agrochemicals, and groundwater abstractions for irrigation and drinking uses. Hexavalent chromium (Cr+6), chromium total (Crtotal), manganese (Mn), and nitrate (NO3-) contamination of groundwater bodies are attributed to both anthropogenic and lithological sources. Elevated Crtotal (up to 70.3 µg L-1), Mn (up to 87.7 µg L-1), and NO3- (up to 411 mg L-1) contents are recorded for groundwater samples in Megara basin. High Crtotal (up to 34.3 µg L-1), Cr6+ (up to 27.9 µg L-1), Mn (up to 132.5 µg L-1), and NO3- (up to 30 mg L-1) are also observed for groundwater samples in the Oropos-Kalamos basin. The major response actions needed for the management options of groundwater bodies are discussed. Among the proposed remedial measures, the installation of a continuous groundwater monitoring network and the control in the usage of nitrogen fertilizers seems to be the most effective and tangible for immediate action.

Meteorological patterns and the evolution of West Nile virus in an environmentally stressed Mediterranean area.

The present work investigates the increase of confirmed cases of West Nile virus and the relationship between weather-related patterns and the geographical expansion of West Nile virus in Greece, with a special focus on West Attica, Central Greece, a semi-arid, ecologically fragile Mediterranean area. Using data from the European Environment Agency, European Drought Observatory of Joint Research Centre, the pairwise relationship between surface air temperature anomalies, precipitation anomalies, soil moisture index anomalies, and the fraction of absorbed photosynthetically active radiation anomalies (fAPAR) was evaluated during summer time of 2018, a particularly intense virus outbreak. The empirical results of this study indicate that total precipitation during 2018 was extremely high, nearly 500% above the average. These conditions contributed to the increase of soil moisture index anomaly and fAPAR, creating an ideal microenvironment (wet soils and green pastures) for mosquito breeding. This phenomenon was directly associated with a drastic outbreak of West Nile virus cases in the area, compared with earlier years. Our results indicate how unusually high values of summer precipitation may have contributed (both through direct and indirect ecological channels) to the rapid spread of the West Nile virus in West Attica, causing a significant number of confirmed cases and fatalities. Climate change may bring forth other issues aside from natural disasters, including-but not limited to-virus expansion.

Suburban areas in flames: Dispersion of potentially toxic elements from burned vegetation and buildings. Estimation of the associated ecological and human health risk.

The results of the assessment of burned residential and wildland areas associated with the July 2018 western Attica wildfire are summarised. The dispersion of major and trace elements in a fire impacted suburban landscape which is adjacent in the north with a Natura 2000 area is evaluated. The dataset includes 35 elements and 27 sampling sites spatially distributed in wildland and residential areas. Field observations and a macroscopic method were applied for investigating the wildfire severity. Statistical and spatial analysis tools were used for data treatment. Major and trace element contents were compared to levels and criteria provided by the United States Environmental Protection Agency, Canadian Council of Ministers of the Environment and Australian Department of Environment and Conservation. Aluminum, As, Co, Fe, Mn, Ni, Sb and Zn concentrations observed in wildfire ash pose potential risk to human health. Median ash concentrations for Al, As, B, Ba, Cd, Cu, Fe, Mn, Ni, Pb, S, Sb, and Zn exceeds the plant-avian-mammalian screening levels in wildland and residential areas. Geogenic or anthropogenic origin of the elements is discussed. The associated health risk on human health and terrestrial ecological receptors (plant, avian, mammalian) is assessed.

Potential of Virtual Earth Observation Constellations in Archaeological Research.

Earth observation sensors continually provide datasets with different spectral and spatial characteristics, while a series of pre- and postprocessing techniques are needed for calibration purposes. Nowadays, a variety of satellite images have become accessible to researchers, while big data cloud platforms allow them to deal with an extensive number of datasets. However, there is still difficulty related to these sensors meeting specific needs and challenges such as those of cultural heritage and supporting archaeological research world-wide. The harmonization and synergistic use of different sensors can be used in order to maximize the impact of earth observation sensors and enhance their benefit to the scientific community. In this direction, the Committee on Earth Observation Satellites (CEOS) has proposed the concept of virtual constellations, which is defined as "a coordinated set of space and/or ground segment capabilities from different partners that focuses on observing a particular parameter or set of parameters of the Earth system". This paper provides an overview of existing and future earth observation sensors, the various levels of interoperability as proposed by Wulder et al., and presents some preliminary results from the Thessalian plain in Greece using integrated optical and radar Sentinel images. The potential for archaeolandscape studies using virtual constellations is discussed here.

Tree uptake of excess nutrients and herbicides in a maize-olive tree cultivation system.

Due to the extent of non-point source agricultural pollution, protective measures to control agrochemicals from entering aquatic systems are necessary. Measures may include, among others, vegetated buffer strips (VFS), no spray buffer zones, alley crops and agroforestry systems (AFS). The scope of the present work is to examine the pollution abatement potential of a maize-olive (MO) AFS. The efficiency of a combined MO trees system in reducing nutrients and herbicides is tested through soil monitoring in an experimental plot, located in Koropi, Eastern Attica, Greece, in the period May 2015 to November 2015. The monitored pollutants were nitrogen and phosphorus, as well as two herbicides: pendimethalin and nicosulfuron. Soil samples were collected every 3-5 weeks at various soil horizons and distances from the tree row. Pollutant concentrations were determined using liquid chromatography tandem mass spectrometry (LC-MS/MS), ion chromatography (IC) and spectrophotometry techniques. The studied MO tree system exhibited the potential to reduce pollutant migration, with removals ranging 36.8-78.9% for [Formula: see text], 79.3-100% for [Formula: see text], 76.7-100% for [Formula: see text], 79.4-100% for [Formula: see text] and 70-100% for the examined herbicides. The higher removal percentages were observed in the upper soil layers (5-35 cm, below the crops), and where the finer tree roots extend. Thus, the results indicate that planting of trees in cultivated fields can contribute to the reduction of agrochemical pollution of the subsurface soil and in extension of groundwater.

Soil Moisture Content Estimation Based on Sentinel-1 and Auxiliary Earth Observation Products. A Hydrological Approach.

A methodology for elaborating multi-temporal Sentinel-1 and Landsat 8 satellite images for estimating topsoil Soil Moisture Content (SMC) to support hydrological simulation studies is proposed. After pre-processing the remote sensing data, backscattering coefficient, Normalized Difference Vegetation Index (NDVI), thermal infrared temperature and incidence angle parameters are assessed for their potential to infer ground measurements of SMC, collected at the top 5 cm. A non-linear approach using Artificial Neural Networks (ANNs) is tested. The methodology is applied in Western Crete, Greece, where a SMC gauge network was deployed during 2015. The performance of the proposed algorithm is evaluated using leave-one-out cross validation and sensitivity analysis. ANNs prove to be the most efficient in SMC estimation yielding R² values between 0.7 and 0.9. The proposed methodology is used to support a hydrological simulation with the HEC-HMS model, applied at the Keramianos basin which is ungauged for SMC. Results and model sensitivity highlight the contribution of combining Sentinel-1 SAR and Landsat 8 images for improving SMC estimates and supporting hydrological studies.

Adipose tissue-derived mesenchymal cells support skin reepithelialization through secretion of KGF-1 and PDGF-BB: comparison with dermal fibroblasts.

Epidermal organization and homeostasis are regulated by mesenchymal influences through paracrine actions. Until today, dermal fibroblasts (DFs) are used in the "dermal" layer to support keratinocyte growth in vitro in dermal and skin substitutes. In the present work, we used human adipose tissue-derived mesenchymal cells (ADMCs) as a support of keratinocyte growth in vitro (in monolayer culture and in 3D skin cell culture models) and in vivo (mouse wound healing models) and compared our findings with those obtained using dermal fibroblasts. ADMCs induce reepithelialization during wound healing more efficiently than DFs, by enhancing keratinocyte proliferation through cell cycle progression, and migration. This effect is mediated (at least partially) by a paracrine action of KGF-1 and PDGF-BB, which are more prominently expressed in ADMCs than in DFs. Furthermore, replacement of DFs by ADMCs in the dermal compartment of organotypic skin cultures leads to an artificial epidermis resembling to that of normal skin, concerning the general histology, although with a higher expression of cytokeratins 5 and 19. In Rag1 knockout mice, ADMCs induced a more rapid reepithelialization and a more effective wound healing, compared to dermal fibroblasts. In conclusion, we provide evidence that ADMCs can serve as supportive cells for primary keratinocyte cultures. In addition, because of their abundance and the great cell yield achieved during ADMC isolation, they represent an interesting cell source, with potential aspects for clinical use.

Detection of neolithic settlements in thessaly (Greece) through multispectral and hyperspectral satellite imagery.

Thessaly is a low relief region in Greece where hundreds of Neolithic settlements/tells called magoules were established from the Early Neolithic period until the Bronze Age (6,000 - 3,000 BC). Multi-sensor remote sensing was applied to the study area in order to evaluate its potential to detect Neolithic settlements. Hundreds of sites were geo-referenced through systematic GPS surveying throughout the region. Data from four primary sensors were used, namely Landsat ETM, ASTER, EO1 - HYPERION and IKONOS. A range of image processing techniques were originally applied to the hyperspectral imagery in order to detect the settlements and validate the results of GPS surveying. Although specific difficulties were encountered in the automatic classification of archaeological features composed by a similar parent material with the surrounding landscape, the results of the research suggested a different response of each sensor to the detection of the Neolithic settlements, according to their spectral and spatial resolution.