RESUMO
The aim of this study was to evaluate a number of foot-and-mouth disease (FMD) test methods for use in red deer. Ten animals were intranasally inoculated with the FMD virus (FMDV) O UKG 11/2001, monitored for clinical signs, and samples taken regularly (blood, serum, oral swabs, nasal swabs, probang samples and lesion swabs, if present) over a 4-week period. Only one animal, deer 1103, developed clinical signs (lesions under the tongue and at the coronary band of the right hind hoof). It tested positive by 3D and IRES real-time reverse transcription polymerase chain reaction (rRT-PCR) in various swabs, lesion materials and serum. In a non-structural protein (NSP) in-house ELISA (NSP-ELISA-IH), one commercial ELISA (NSP-ELISA-PR) and a commercial antibody NSP pen side test, only deer 1103 showed positive results from day post-inoculation (dpi) 14 onwards. Two other NSP-ELISAs detected anti-NSP serum antibodies with lower sensitivity. It also showed rising antibody levels in the virus neutralization test (VNT), the in-house SPO-ELISA-IH and the commercial SPO-ELISA-PR at dpi 9, and in another two commercial SPO-ELISAs at dpi 12 (SPO-ELISA-IV) and dpi 19 (SPO-ELISA-IZ), respectively. Six of the red deer that had been rRT-PCR and antibody negative were re-inoculated intramuscularly with the same O-serotype FMDV at dpi 14. None of these animals became rRT-PCR or NSP-ELISA positive, but all six animals became positive in the VNT, the in-house SPO-ELISA-IH and the commercial SPO-ELISA-PR. Two other commercial SPO-ELISAs were less sensitive or failed to detect animals as positive. The rRT-PCRs and the four most sensitive commercial ELISAs that had been used for the experimentally inoculated deer were further evaluated for diagnostic specificity (DSP) using 950 serum samples and 200 nasal swabs from non-infected animals. DSPs were 100% for the rRT-PCRs and between 99.8 and 100% for the ELISAs.
Assuntos
Cervos , Testes Diagnósticos de Rotina/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Vírus da Febre Aftosa/isolamento & purificação , Febre Aftosa/diagnóstico , Proteínas não Estruturais Virais/análise , Animais , Anticorpos Antivirais/sangue , Testes Diagnósticos de Rotina/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Febre Aftosa/virologia , Vírus da Febre Aftosa/imunologia , Masculino , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterináriaRESUMO
In January 2014, approximately 9 months following the initial detection of porcine epidemic diarrhea (PED) in the USA, the first case of PED was confirmed in a swine herd in south-western Ontario. A follow-up epidemiological investigation carried out on the initial and 10 subsequent Ontario PED cases pointed to feed as a common risk factor. As a result, several lots of feed and spray-dried porcine plasma (SDPP) used as a feed supplement were tested for the presence of PEDV genome by real-time RT-PCR assay. Several of these tested positive, supporting the notion that contaminated feed may have been responsible for the introduction of PEDV into Canada. These findings led us to conduct a bioassay experiment in which three PEDV-positive SDPP samples (from a single lot) and two PEDV-positive feed samples supplemented with this SDPP were used to orally inoculate 3-week-old piglets. Although the feed-inoculated piglets did not show any significant excretion of PEDV, the SDPP-inoculated piglets shed PEDV at a relatively high level for ≥9 days. Despite the fact that the tested PEDV genome positive feed did not result in obvious piglet infection in our bioassay experiment, contaminated feed cannot be ruled out as a likely source of this introduction in the field where many other variables may play a contributing role.
Assuntos
Ração Animal/virologia , Infecções por Coronavirus/veterinária , Diarreia/veterinária , Surtos de Doenças/veterinária , Vírus da Diarreia Epidêmica Suína , Doenças dos Suínos/etiologia , Animais , Canadá/epidemiologia , Infecções por Coronavirus/virologia , Diarreia/epidemiologia , Diarreia/virologia , Contaminação de Alimentos , Dados de Sequência Molecular , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
We tested, using a low starting dilution, sequential serum samples from dromedary camels, sheep and horses collected in Dubai from February/April to October of 2005 and from dromedary camels for export/import testing between Canada and USA in 2000-2001. Using a standard Middle East respiratory syndrome coronavirus (MERS-CoV) neutralization test, serial sera from three sheep and three horses were all negative while sera from 9 of 11 dromedary camels from Dubai were positive for antibodies supported by similar results in a MERS-CoV recombinant partial spike protein antibody ELISA. The two negative Dubai camels were both dromedary calves and remained negative over the 5 months studied. The six dromedary samples from USA and Canada were negative in both tests. These results support the recent findings that infection with MERS-CoV or a closely related virus is not a new occurrence in camels in the Middle East. Therefore, interactions of MERS-CoV at the human-animal interface may have been ongoing for several, perhaps many, years and by inference, a widespread pandemic may be less likely unless significant evolution of the virus allow accelerated infection and spread potential in the human population.
Assuntos
Anticorpos Antivirais/sangue , Camelus/virologia , Infecções por Coronavirus/veterinária , Coronavirus/isolamento & purificação , Animais , Coronavirus/imunologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Cavalos , Incidência , Oriente Médio , Testes de Neutralização , Ovinos , Síndrome , Emirados Árabes Unidos/epidemiologiaRESUMO
White tailed deer (Odocoileus virginianus) were inoculated with foot-and-mouth disease virus (FMDV) O UKG 11/2001 and monitored for the development of clinical signs, histopathological changes and levels of virus replication. All FMDV-infected deer developed clinical signs starting at 2 days post inoculation and characterized by an increase in body temperature, increased salivation and lesions in the mouth and on the feet. Virus spread to various tissues was determined by quantifying the amount of FMDV RNA using quantitative reverse transcriptase polymerase chain reaction. Virus or viral antigen was also detected in tissues using traditional isolation techniques, enzyme linked immunosorbent assay and immunohistochemistry. Deer-to-cattle transmission of the virus was observed in this experimental setting; however, inoculated deer were not found to become carriers of FMDV.
Assuntos
Cervos/virologia , Vírus da Febre Aftosa/patogenicidade , Febre Aftosa/patologia , Animais , Animais Selvagens/virologia , Bovinos , Cervos/imunologia , Modelos Animais de Doenças , Transmissão de Doença Infecciosa , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Febre Aftosa/transmissão , Vírus da Febre Aftosa/genética , Vírus da Febre Aftosa/imunologia , Vírus da Febre Aftosa/isolamento & purificação , Imuno-Histoquímica/veterinária , Transmissão Vertical de Doenças Infecciosas , Masculino , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Replicação ViralRESUMO
The myxozoan genus Parvicapsula contains 14 species infecting fish, some of which are known to cause severe disease in farmed and wild salmonids. Parvicapsula pseudobranchicola infections were first reported from seawater-reared Atlantic salmon, Salmo salar, in Norway in 2002 and have since then been an increasing problem. The present study describes a Taqman real-time PCR assay for specific detection of P. pseudobranchicola. The Taqman assay targets the 18S rRNA gene of P. pseudobranchicola and is able to detect as few as ten copies of the target sequence. Using the described assay, P. pseudobranchicola was detected in both farmed and wild salmonids, indicating that wild Atlantic salmon, sea trout, Salmo trutta, and Arctic char, Salvelinus alpinus, may be natural hosts of the parasite. Parvicapsula pseudobranchicola was found in samples from wild salmonids in the far south and the far north of Norway, displaying a wide geographic range of the parasite. Farmed salmonids showed P. pseudobranchicola infection levels many folds higher than that observed for wild sea trout, indicating that farmed Atlantic salmon are subjected to an elevated infection pressure compared with wild salmonids.
Assuntos
Doenças dos Peixes/diagnóstico , Myxozoa/isolamento & purificação , Doenças Parasitárias em Animais/diagnóstico , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Salmonidae/parasitologia , Animais , Doenças dos Peixes/epidemiologia , Pesqueiros , Peixes , Noruega , Doenças Parasitárias em Animais/epidemiologia , Sensibilidade e Especificidade , Análise de Sequência de DNA/veterináriaRESUMO
There are many benefits that derive from real-time knowledge of the health status of the national livestock population. Effective animal disease surveillance is a requirement for countries that trade in live animals and their products in order to comply with the World Organization for Animal Health (OIE) guidelines. Rapid identification of introduced and emerging disease allows rapid response and mitigation of the economic consequences. Connections between animal and human disease caused by a common pathogen can be recognized and control measures implemented, thereby protecting public health and maintaining public confidence in the food supply. Production-limiting diseases can be monitored, and control programmes be evaluated with benefits accruing from decreased economic losses associated with disease as well as reducing the welfare concerns associated with diseased animals. Establishing a surveillance programme across a wide area with diverse ecosystems and political administrations as Canada is a complex challenge. When funding became available from a government programme to enable early detection of a bio-terrorist attack on livestock, the Canadian Animal Health Surveillance Network (CAHSN) became officially established. An existing web-based information platform that supports intelligence exchange, surveillance and response for public health issues in Canada was adapted to link the network animal health laboratories. A minimum data set was developed that facilitated sharing of results between participating laboratories and jurisdictions as the first step in creating the capacity for national disease trend analysis. In each of the network laboratories, similar quality assurance and bio-containment systems have been funded and supported, and diagnostic staff have been trained and certified on a suite of diagnostic tests for foreign animal diseases. This ensures that national standards are maintained throughout all of the diagnostic laboratories. This paper describes the genesis of CAHSN, its current capability and governance, and potential for future development.
Assuntos
Controle de Doenças Transmissíveis/métodos , Gado , Sistemas de Informação Administrativa , Prática de Saúde Pública , Vigilância de Evento Sentinela/veterinária , Animais , Canadá , Controle de Doenças Transmissíveis/instrumentação , Surtos de Doenças/prevenção & controle , Internet , Relações InterprofissionaisRESUMO
Uganda had an unusually large number of foot-and-mouth disease (FMD) outbreaks in 2006, and all clinical reports were in cattle. A serological investigation was carried out to confirm circulating antibodies against foot-and-mouth disease virus (FMDV) by ELISA for antibodies against non-structural proteins and structural proteins. Three hundred and forty-nine cattle sera were collected from seven districts in Uganda, and 65% of these were found positive for antibodies against the non-structural proteins of FMDV. A subset of these samples were analysed for serotype specificity of the identified antibodies. High prevalences of antibodies against non-structural proteins and structural proteins of FMDV serotype O were demonstrated in herds with typical visible clinical signs of FMD, while prevalences were low in herds without clinical signs of FMD. Antibody titres were higher against serotype O than against serotypes SAT 1, SAT 2 and SAT 3 in the sera investigated for serotype-specific antibodies. Only FMDV serotype O virus was isolated from one probang sample. This study shows that the majority of the FMD outbreaks in 2006 in the region studied were caused by FMDV serotype O; however, there was also evidence of antibodies to both SAT 1 and SAT 3 in one outbreak in a herd inside Queen Elizabeth national park area.
Assuntos
Doenças dos Bovinos/virologia , Vírus da Febre Aftosa/imunologia , Febre Aftosa/imunologia , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Febre Aftosa/epidemiologia , Febre Aftosa/virologia , Uganda/epidemiologiaRESUMO
In East Africa, the foot-and-mouth disease (FMD) virus (FMDV) isolates have over time included serotypes O, A, C, Southern African Territories (SAT) 1 and SAT 2, mainly from livestock. SAT 3 has only been isolated in a few cases and only in African buffalos (Syncerus caffer). To investigate the presence of antibodies against FMDV serotypes in wildlife in Uganda, serological studies were performed on buffalo serum samples collected between 2001 and 2003. Thirty-eight samples from African buffalos collected from Lake Mburo, Kidepo Valley, Murchison Falls and Queen Elizabeth National Parks were screened using Ceditest FMDV NS to detect antibodies against FMDV non-structural proteins (NSP). The seroprevalence of antibodies against non-structural proteins was 74%. To characterize FMDV antibodies, samples were selected and titrated using serotype-specific solid phase blocking enzyme linked immunosorbent assay (ELISAs). High titres of antibodies (> or =1 : 160) against FMDV serotypes SAT 1, SAT 2 and SAT 3 were identified. This study suggests that African buffalos in the different national parks in Uganda may play an important role in the epidemiology of SAT serotypes of FMDV.
Assuntos
Anticorpos Antivirais/sangue , Búfalos , Vírus da Febre Aftosa/imunologia , Febre Aftosa/epidemiologia , Animais , Febre Aftosa/imunologia , Vírus da Febre Aftosa/classificação , Estudos Soroepidemiológicos , Sorotipagem/veterinária , Uganda/epidemiologiaRESUMO
This article reviews the options for use of virus detection techniques for decentralized testing of samples from suspected secondary outbreaks of foot-and-mouth disease (FMD). These options have been expanded by the advent of new tests including disposable lateral flow devices (LFDs) that detect viral proteins and portable RT-PCR equipment that detects viral RNA. LFDs have been developed with similar sensitivity to antigen detection ELISA but with the ability to provide a result 1-30 min after the addition of epithelium or vesicular fluid. Portable RT-PCR platforms are being developed that can detect FMD viral RNA in blood, epithelium or other materials with minimal sample processing and with high sensitivity, in as little as 60 min in some cases. These devices may be used on infected farms as pen-side tests, in regional, local or mobile laboratories, or in National Reference Laboratories (NRL). Advantages and disadvantages of different testing options are considered to inform decisions on the optimal strategies for different national circumstances. Issues include validation and quality control, containment needs, availability of test devices and reagents, the decision tree for declaring an outbreak, training issues and provision of samples for subsequent viral characterization. Tests to confirm the diagnosis of the index case of an outbreak of FMD should continue to be carried out in the NRL.
Assuntos
Surtos de Doenças/veterinária , Febre Aftosa/diagnóstico , Febre Aftosa/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Animais , Sistemas Automatizados de Assistência Junto ao Leito , Reprodutibilidade dos TestesRESUMO
The progress and pathogenesis of foot-and-mouth disease virus (FMDV) was studied in infected pigs by observing the development of clinical signs in two separate experiments. Viral loads were determined by real-time quantitative RT-PCR in the liver, spleen, cervical lymph node, mandibular lymph node, retropharyngeal lymph node, soft palate, pharynx, tonsil, tongue and skin (coronary band area). Tissue samples were collected from both inoculated and contact-infected pigs at several time points during infection, and blood samples were collected to assess viraemia and its relationship to tissue viral load. Virus first appeared in the lymph nodes, followed by viraemia and then clinical signs. The results suggested that FMDV accumulated in lymphoid tissue up to six hours after infection, in the tissues drained by the mandibular lymph node and tonsil and then disseminated throughout the body where epithelial cells were the favoured sites of replication.
Assuntos
Vírus da Febre Aftosa/fisiologia , Febre Aftosa/virologia , Doenças dos Suínos/virologia , Carga Viral/veterinária , Doença Aguda , Animais , Febre Aftosa/transmissão , Vírus da Febre Aftosa/genética , Vírus da Febre Aftosa/isolamento & purificação , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Suínos , Doenças dos Suínos/transmissão , Viremia/veterinária , Replicação ViralRESUMO
Foot-and-mouth disease (FMD) is endemic in Uganda with control strategies focusing on vaccination of cattle, while small ruminants are largely ignored. In order for Uganda to establish effective control strategies, it is crucial that the epidemiology of the disease is fully understood. This study summarizes results of serological investigations of sheep and goats for antibodies to FMDV from four districts in 2006 following an FMD outbreak in the region and from an attempted comprehensive random sampling in two districts in 2007. Antibodies were quantified and serotyped using competitive ELISA for antibodies towards non-structural proteins (NSP) and structural proteins towards serotype O, and blocking ELISA for antibodies towards the seven serotypes of FMD virus (FMDV). In 2006, sheep and goats in Bushenyi and Isingiro districts were free from antibodies towards FMDV, while herds in Kasese and Mbarara districts excluding Kahendero village were all positive for antibodies towards NSP and SP-O. In 2007, mean prevalence estimates of antibodies towards FMDV NSP was 14% in goats and 22% in sheep in Kasese district, while Bushenyi was still free. The difference between these two districts probably reflects different levels of FMDV challenge attributed to the variation in exposure rates which again in part may be as a result of the differing husbandry practices. Contrary to 2006, with clear antibodies towards serotype O, the serotype-specificity of the antibodies was less clear in 2007, as antibodies towards both serotype O and SAT serotypes were identified. Our results show that goats and sheep are infected during FMD outbreaks, and that they may be useful for determining the serotype of FMD outbreaks in Uganda, if they are sampled shortly after an outbreak.
Assuntos
Anticorpos Antivirais/sangue , Vírus da Febre Aftosa/imunologia , Febre Aftosa/imunologia , Animais , Surtos de Doenças/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Febre Aftosa/epidemiologia , Cabras , Prevalência , Ovinos , Uganda/epidemiologia , Proteínas não Estruturais Virais/imunologiaRESUMO
Foot-and-mouth disease virus (FMDV) can be spread by direct animal-to-animal contact, indirect contact facilitated by contaminated materials or by airborne spread. The rate of spread and the incubation period, as well as the severity of disease, depends on many variables including the dose received, the route of introduction, the virus strain, the animal species and the conditions under which the animals are kept. Quantitative data related to these variables are needed if model predictions are to be used in practical disease control. This experimental study quantifies the risk of transmission of FMDV in pigs exposed by contact, sheep exposed by indirect contact with pigs and sheep exposed to airborne FMDV. Groups of pigs were inoculated with the FMDV O UKG 34/2001 strain and susceptible pigs were then exposed to the inoculated animals at different stages of the infection cycle. The mean incubation period in the susceptible pigs ranged from 1 to 10 days. The length of the incubation period, severity of clinical disease and efficiency of spread were related to dose (i.e. infectiousness of source and intensity of contact). Low intensity transmission increased the proportion of subclinical or abortive infections. Local conditions are important in the efficiency and speed of transmission of FMDV. The results of the experiments described above suggest that transmission is frequency dependent rather than density dependent. The sheep experiments provided further evidence that development of infection and clinical disease is dependent upon local conditions. Dose, infectiousness, intensity of contact and local factors are thus important determinants for the outcome of an initial outbreak and must be truthfully accounted for in mathematical models of epidemiological spread.
Assuntos
Transmissão de Doença Infecciosa/veterinária , Vírus da Febre Aftosa/patogenicidade , Febre Aftosa/transmissão , Doenças dos Ovinos/transmissão , Doenças dos Suínos/transmissão , Microbiologia do Ar , Animais , Bovinos , Células Cultivadas , Abrigo para Animais , Exposição por Inalação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Índice de Gravidade de Doença , Ovinos , Doenças dos Ovinos/virologia , Especificidade da Espécie , Suínos , Doenças dos Suínos/virologia , Glândula Tireoide/citologia , Glândula Tireoide/virologia , Fatores de Tempo , Carga Viral/métodos , Viremia/transmissão , Viremia/veterináriaRESUMO
In this study, two sheep, eight dromedary camels and two Bactrian camels were inoculated with foot-and-mouth disease virus (FMDV) type A SAU 22/92. Five naive dromedary camels and four sheep were kept in direct or indirect contact with the inoculated camels. The inoculated sheep, which served as positive controls, displayed typical moderate clinical signs of FMD and developed viraemia and high antibody titres. The presence of the virus was also detected in probang and mouth-swab samples for several days after inoculation. In contrast, the inoculated dromedary camels were not susceptible to FMDV type A infection. None of them showed clinical signs of FMD or developed viraemia or specific anti-FMDV antibodies despite the high dose of virus inoculated. All the contact sheep and contact dromedaries that were kept together with the inoculated camels remained virus-negative and did not seroconvert when tested up to 28 days post-inoculation (p.i.). In comparison with the non-susceptible dromedaries, the two inoculated Bactrian camels showed moderate to severe clinical signs of FMD; however, the clinical signs of FMD appeared rather late, between 8 and 14 days p.i., compared to the inoculated sheep. Characteristic FMD lesions in the Bactrian camels, accompanied with severe lameness, were only observed on the hind feet. The presence of the virus in the serum samples of both Bactrian camels was detected by real-time RT-PCR in one of the animals on days 3 and 7 p.i. and in the second animal from days 1 to 3 p.i. and subsequently again on day 21 p.i. The Bactrian camels developed high titres of antibodies to the inoculated FMDV which appeared at 7-10 days p.i. and lasted up to 130 days p.i. Only low and transient amounts of FMDV were detected in the mouth-swab and probang samples collected from both Bactrian camels.
Assuntos
Camelus , Febre Aftosa/imunologia , Animais , Anticorpos Antivirais/sangue , Suscetibilidade a Doenças/veterinária , Febre Aftosa/virologia , Vírus da Febre Aftosa/imunologia , Vírus da Febre Aftosa/isolamento & purificação , Masculino , Ovinos , Doenças dos Ovinos/virologia , Fatores de TempoRESUMO
Ten male Arabian oryx (Oryx leucoryx) were vaccinated with a commercially available standard aqueous foot-and-mouth-disease vaccine containing aluminium hydroxide as an adjuvant, and their antibody titres against serotypes O and A were measured using solid-phase blocking elisa and the virus neutralisation test. Mean elisa antibody titres greater than 1.45 log(10) were recorded for serotype A, but low elisa titres were recorded for serotype 0; low titres were recorded by VNT for both serotypes.
Assuntos
Antílopes , Anticorpos Antivirais/sangue , Vírus da Febre Aftosa/imunologia , Febre Aftosa/prevenção & controle , Vacinas Virais/imunologia , Animais , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Vírus da Febre Aftosa/classificação , Masculino , Testes de Neutralização/métodos , Testes de Neutralização/veterinária , SorotipagemRESUMO
Two sheep and five dromedaries were inoculated with a highdose of a cattle-passaged type O strain of foot-and-mouth disease virus (FMDV). The sheep developed typical FMD. The inoculated camels, which were placed in contact with five further dromedaries and four sheep, showed no visible signs of illness or vesicular lesions. However, one of them had a raised body temperature at 3 days post-inoculation (pi) and a viraemia from days 2 to 10; probang samples from this animal were negative for infectious virus, but a low level of FMDV RNA was detected in a sample taken on day 6 pi, five other samples taken from days 3 to 28 being negative. Examination of mouth swabs indicated a low level of FMDV RNA at days 1-5 pi in four of the five inoculated camels, but no infectious FMDV or FMDV RNA was detected in serum, probang or mouth swab samples from contact-exposed animals (camels and sheep). All the contact-exposed camels and sheep and two of the inoculated camels were serologically negative for FMD when tested up to day 28. In contrast, the camel with viraemia became serologically positive from day 14, and the other two inoculated camels (which had been exposed to FMDV in an earlier experiment) became serologically positive from day 10. The experiment suggested that dromedaries (1) are of low susceptibility to FMDV serotype O, (2) do not transmit infection, even by close contact, and (3) are unlikely to play a significant epidemiological role in FMD.
Assuntos
Camelus/virologia , Suscetibilidade a Doenças/veterinária , Vírus da Febre Aftosa/patogenicidade , Febre Aftosa/epidemiologia , Febre Aftosa/transmissão , Animais , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Ovinos/virologia , Carneiro Doméstico/virologiaRESUMO
Although the pathogenesis of foot-and-mouth disease (FMD) has been extensively investigated, relatively few studies have addressed the localization of FMD virus (FMDV) and in particular its replication in relation to the typical in-vivo sites of FMD lesions. In the present study, pigs were infected experimentally with FMDV serotype O UKG 34/2001 and tissue samples were collected from 1 to 4 days post-infection. Samples were stored at -70 degrees C and frozen sections were prepared for in-situ hybridization (ISH). A digoxigenin-labelled RNA probe complementary to a coding part of the RNA-dependent RNA polymerase (3D) genomic region was prepared. The FMDV positive strand RNA was prominent in the basal layers of the epithelium. A diffuse positive signal was also noted in the cytoplasm of cells of the stratum spinosum of lesional epithelium, but there was no signal in the stratum corneum. Detection of FMDV negative strand RNA was observed in basal cells above the basement membrane and along the dermal papillae. The basal cells therefore demonstrate the highest signal for detection of the FMDV positive and negative strand RNAs in both tongue and foot epithelium. These novel results suggest that the epithelial basal cells could be an early replication site of FMDV in vivo.
Assuntos
Células Epiteliais/virologia , Vírus da Febre Aftosa/fisiologia , Febre Aftosa/virologia , Doenças dos Suínos/virologia , Suínos/virologia , Animais , Imuno-Histoquímica , Hibridização In Situ , RNA Viral/isolamento & purificação , Replicação Viral/genéticaRESUMO
Speed is paramount in the diagnosis of foot-and-mouth disease (FMD) and simplicity is required if a test is to be deployed in the field. The development of a one-step, reverse transcription loop-mediated amplification (RT-LAMP) assay enables FMD virus (FMDV) to be detected in under an hour in a single tube without thermal cycling. A fragment of the 3D RNA polymerase gene of the virus is amplified at 65 degrees C in the presence of a primer mixture and both reverse transcriptase and Bst DNA polymerase. Compared with real-time RT-PCR, RT-LAMP was consistently faster, and ten copies of FMDV transcript were detected in twenty-two minutes. Amplification products were detected by visual inspection, agarose gel electrophoresis, or in real-time by the addition of a fluorescent dye. The specificity of the reaction was demonstrated by the absence of amplification of RNA from other viruses that cause vesicular diseases and from that of genetically related picornaviruses. Diagnostic sensitivity was validated by the amplification of reference FMDV strains and archival material from field cases of FMD. In comparison with the performance of the established diagnostic TaqMan assay, RT-LAMP appears to be sensitive, rapid, specific, and cost-effective, with the potential for field deployment and use by developing countries for FMDV surveillance.
Assuntos
Vírus da Febre Aftosa/isolamento & purificação , Febre Aftosa/diagnóstico , Técnicas de Amplificação de Ácido Nucleico , RNA Viral/análise , Animais , Antígenos Virais/genética , Primers do DNA , RNA Polimerases Dirigidas por DNA/genética , Eletroforese em Gel de Ágar , Fluorescência , Vírus da Febre Aftosa/genética , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Temperatura , Fatores de Tempo , Proteínas não Estruturais Virais/genéticaRESUMO
The pharyngeal region is known to play an important role in foot-and-mouth disease virus (FMDV) infection in relation to acute disease and viral persistence. In this study, the local mucosal immune response in nasal-associated lymphoid tissue (NALT) of cattle infected with FMDV (strain O UKG 34/2001) was examined. Quantitative "real-time" RT-PCR assays were used to measure mRNA expression of cytokines (IFN-alpha, beta and gamma, IL-2, IL-1alpha and TNF-alpha) and Toll-like receptors (TLR)-3 and -4. NALTs from dorsal soft palate were collected from cattle at 7 days post-infection (dpi) and from carriers and non-carriers at 64 dpi. Expression of IFN-alpha mRNA was significantly greater in NALT during acute disease than in uninfected animals. Increased expression of IFN-gamma and IL-1alpha mRNA was also observed but was much lower than IFN-alpha expression. There was a slight increase in mRNA expression of TNF-alpha and IL-2. During persistence, TNF-alpha mRNA expression in carrier cattle was much higher than in non-carrier cattle. Expression of TLR-4 in NALT during the acute stage of infection was greater than in uninfected animals. Carrier and non-carrier cattle did not differ in respect of expression of TLR-3 and -4 mRNA in NALT.
Assuntos
Doenças dos Bovinos/imunologia , Febre Aftosa/imunologia , Tecido Linfoide/metabolismo , Receptores de Citocinas/metabolismo , Receptores Toll-Like/metabolismo , Animais , Bovinos , Tecido Linfoide/imunologia , Mucosa Nasal/metabolismo , RNA Mensageiro/metabolismo , Receptores de Interleucina/metabolismo , Receptores do Fator de Necrose Tumoral , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 3 Toll-Like/metabolismo , Receptor 4 Toll-Like/metabolismoRESUMO
The likelihood of airborne spread of foot-and-mouth disease at the start of the 1967-1968 epidemic is re-assessed in the light of current understanding of airborne disease spread. The findings strongly confirm those made at the time that airborne virus was the most likely cause of the rapid early development of the disease out to 60 km from the source. This conclusion is reached following a detailed epidemiological, meteorological and modelling study using original records and current modelling techniques. The role played by 'lee waves' as the mechanism for the spread is investigated. It is thought that they played little part in influencing the development of the epidemic. A number of lessons learned from the work are drawn, identifying the need for further research on the quantity and characteristics of airborne virus. The results are also used to illustrate what advice would have been available to disease controllers if the outbreak had occurred in 2004.