RESUMO
Pre- and postalpha-exposed PM-355 detectors were irradiated using UV laser with different number of pulses (100, 150, 200, 300, and 400). UV laser beam energy of 20mJ per pulse with a pulse width of 9ns was incident on an area of 19.6mm2 of the samples. XRD spectra indicated that for both reference and UV-irradiated samples, the structure is amorphous, but the crystallite size increases upon UV irradiation. The same results were obtained from SEM analysis. Optical properties of PM-355 polymeric solid-state nuclear track detectors were also investigated. Absorbance measurements for all PM-355 samples in the range of 200-400nm showed that the absorption edge had a blue shift up to a certain value, and then, it had an oscillating behavior. Photoluminescence spectra of PM-355 at 250nm revealed a decrease in the broadband peak intensity as a function of the number of UV pulses, while the wavelengths corresponding to the peaks had random shifts.
RESUMO
Two faba bean, Vicia faba L., cultivars, Gazira2 and Misr1, representing cultivars moderately resistant and susceptible to aphids, were analyzed for peroxidase (POD) and polyphenol oxidase (PPO) activities induced by cowpea aphid, Aphis craccivora Koch. infestation. Two tissue types (whole plant [WP] and detached leaf [DL]), two infestation status (infested and uninfested), and three aphid infestation durations (1, 3, and 5 d) were considered in POD and PPO data analysis. Factorial analysis showed that only cultivar factor has a significant effect on both POD and PPO activity, especially on the first day after aphid infestation (P: 0.0003 and 0.001, respectively). Tissue type has no significant effect, indicating either DL or WP can be used for measuring POD and PPO activities. While the unsignificant different of infestation status reflecting a constitutive resistant character in Gazira2. Mann-Whitney U-test showed that POD and PPO activities in Gazira2 were higher significantly when compared with Misr1 with P value 0.0006 and 0.0015 for POD and PPO, respectively. Repeated measures analysis indicates that the POD and PPO activities on Gazira2 were significantly higher when compared with Misr1. Additionally, POD activity changed significantly over the time in 1, 3, and 5 d after aphid infestation. We concluded that higher activity of POD and PPO in cultivar Gazira2 is strongly associated with their resistant characters.