Evaluation of the Ecological Environment Affected by Cry1Ah1 in Poplar.

Populus is a genus of globally significant plantation trees used widely in industrial and agricultural production. Poplars are easily damaged by Micromelalopha troglodyta and Hyphantria cunea, resulting in decreasing quality. Bt toxin-encoded by the Cry gene has been widely adopted in poplar breeding because of its strong insect resistance. There is still no comprehensive and sufficient information about the effects of Cry1Ah1-modified (CM) poplars on the ecological environment. Here, we sampled the rhizosphere soils of field-grown CM and non-transgenic (NT) poplars and applied 16S rRNA and internal transcribed spacer amplicon Illumina MiSeq sequencing to determine the bacterial community associated with the CM and NT poplars. Based on the high-throughput sequencing of samples, we found that the predominant taxa included Proteobacteria (about 40% of the total bacteria), Acidobacteria (about 20% of the total bacteria), and Actinobacteria (about 20% of the total bacteria) collected from the natural rhizosphere of NT and CM poplars. In addition, studies on the microbial diversity of poplar showed that Cry1Ah1 expression has no significant influence on rhizosphere soil alkaline nitrogen, but significantly affects soil phosphorus, soil microbial biomass nitrogen, and carbon. The results exhibited a similar bacterial community structure between CM varieties affected by the expression of Cry1Ah1 and non-transgenic poplars. In addition, Cry1Ah1 expression revealed no significant influence on the composition of rhizosphere microbiomes. These results broadly reflect the effect of the Bt toxin-encoded by Cry1Ah1 on the ecology and environment and provide a clear path for researchers to continue research in this field in the future.

Postharvest quality of two orange-fleshed sweet potato [Ipomoea batatas (L) Lam] cultivars as influenced by organic soil amendment treatments.

Two orange-fleshed sweet potato cultivars: Apomuden and "Nane" were grown on cow dung-, chicken manure-, compost-amended soils, and untreated soil. Apomuden is a variety, while "Nane" is being evaluated to be released in Ghana. The storage roots (SRs) were harvested at 3 months, cured by heaping the SRs and covering with the sweet potato foliage for 7 days in the field. The cured SRs were kept in an evaporative cool chamber to study the effect of soil amendment treatments on weight loss, rot, some nutrient composition, and sensory attributes. Boiled SRs were assessed by 70 untrained panelists after 7 weeks of storage based on the following: general appearance, sweetness, finger-feel firmness, and overall acceptability using a 5-point hedonic scale (1 = dislike extremely to 5 = like extremely). Percent rot for "Nane" showed a linear trend, while that of Apomuden was nonlinear. Both cultivars showed similar trends in terms of cumulative weight loss with "Nane" recording lower weight loss compared with Apomuden. A significant (p < 0.001; r = 0.71) strong positive correlation was observed between weight loss and rots. "Nane" had higher dry matter (37.15% vs. 30.19%; p < 0.001, respectively) and starch content (59.16% vs. 51.86%; p < 0.001, respectively) than Apomuden. Stored SRs grown on chicken manure-amended soil recorded the highest protein (6.41%; p < 0.001) and ß-carotene (16.64 mg/100 g; p < 0.001) content than the other treatments. There was a 35% decline in ß-carotene for Apomuden, while "Nane" increased by 24% at the end of the 7-week storage. "Nane," the cultivar with high dry matter content had good storage properties than Apomuden. Stored SRs cultivated on soils amended with chicken manure had higher ß-carotene and protein content. All sensory attributes ranged from 3.35 to 3.68 indicating a good consumer preference for both cultivars irrespective of the soil amendment treatment applied.

Caring for Blind Parents: Cost to the Education and Well-Being of Children in Tamale, Ghana.

In this study we explored the experiences of the guide children (children who guide adults with visual impairment to beg for a living on the streets) in Tamale, Ghana, to unearth the impact of the guiding role on their educational participation and well-being. Data were gathered from 104 children and 57 adults using a set of questionnaires and interviews. The results showed that adults chose many of the children to take on the guide role and many of the children did not attend school. The implications for the children's well-being are discussed in relation to needs and rights.

The Ghana community-based rehabilitation program for people with disabilities: what happened at the end of donor support?

In this case study the authors examined the functioning of the community-based rehabilitation (CBR) program for people with disabilities in 3 pilot districts after the conclusion of donor support in Ghana. Questionnaire and interview data from 42 people with disabilities, 8 local supervisors, and 3 social workers about program structures, support for people with disabilities, and challenges were analyzed using descriptive statistics and qualitative procedures, involving the use of Leximancer software. The authors found that some CBR structures remained in the communities. Diminished support for disabled peoples' organizations from communities and local government agencies were key challenges. The problem of volunteer local supervisors wanting to be paid ignited the evidence versus ideology debate around sustainability of CBR programs.