Eco-friendly and cost-effective adsorbent derived from blast furnace slag with black liquor waste for hazardous remediation.

The current investigation concerns with preparation eco-friendly and cost-effective adsorbent (mesoporous silica nanoparticles (SBL)) based on black liquor (BL) containing lignin derived from sugarcane bagasse and combining it with sodium silicate derived from blast furnace slag (BFS) for thorium adsorption. Thorium ions were adsorbed from an aqueous solution using the synthesized bio-sorbent (SBL), which was then assessed by X-ray diffraction, BET surface area analysis, scanning electron microscopy with energy dispersive X-ray spectroscopy (EDX), and Fourier transforms infrared spectroscopy (FTIR). Th(IV) sorption properties, including the pH effect, uptake rate, and sorption isotherms across various temperatures were investigated. The maximum sorption capacity of Th(IV) on SBL is 158.88 mg/L at pH value of 4328 K, and 60 min contact time. We demonstrated that the adsorption processes comport well with pseudo-second-order and Langmuir adsorption models considering the kinetics and equilibrium data. According to thermodynamic inspections results, the Th(IV) adsorption process exhibited endothermic and random behavior suggested by positive ΔH° and ΔS° values, while the negative ΔG° values indicated a spontaneous sorption process. The maximum Th(IV) desorption from the loaded SBL (Th/SBL) was carried out at 0.25 M of NaHCO3 and 60 min of contact. Sorption/desorption processes have five successive cycles. Finally, this study suggests that the recycling of BFS and BL can be exploited for the procurement of a promising Th(IV) adsorbents.

Basic Fuchsin Dye as the First Fluorophore for Optical Sensing of Morpholine in Fruits Crust and Urine Samples.

In this work, basic fuchsin (BF) dyestuff was presented as the first optical sensor used for the spectrofluorimetric assessment of morpholine (MOR) where BF exhibits morpholine-sensing behavior. The developed fluorimetric avenue is sensitive, facile, selective, and validated for assaying the sensitizing influence of MOR on the BF fluorescence in an aprotic dioxane solvent. Parameters like solvents, BF concentration, order, and time of addition that influence the fluorescence intensity of the probing system were addressed. Optimizing the analytical methodology revealed a linear fluorescence sensitization within the addition of MOR in the two concentration ranges of 5 × 10-9 to 1.0 × 10-7 mol L-1 and 2.0 × 10-7 to 3 × 10-6 mol L-1. The limit of detection (LOD) and limit of quantification (LOQ) were estimated to be 2.0 × 10-9 mol L-1 (0.17 ng mL-1) and 6.66 × 10-9 mol L-1 (0.567 ng mL-1), respectively. High levels of accuracy and precision are achieved when assaying spiked MOR either in pure solutions or samples of fruit peel extract and human urine. Moreover, the green character and practicality/applicability of the method were evaluated by AGREE and BAGI metric tools. These merit outcomes provide insights into the development of fluorescent sensors for MOR detection using fluorescent dyes and meet the Food and Drug Administration's requirements for morpholine detection in real-life applications.

A hybrid mesoporous composite of SnO2 and MgO for adsorption and photocatalytic degradation of anionic dye from a real industrial effluent water.

Water pollution by synthetic anionic dyes is one of the most critical ecological concerns and challenges. Therefore, there is an urgent need to find an efficient adsorbent and photocatalyst for dye removal. In the present study, we aimed to fabricate a hybrid mesoporous composite of spongy sphere-like SnO2 and three-dimensional (3D) cubic-like MgO (SnO2/MgO) as a promising adsorbent/photocatalyst to remove the anionic sunset yellow (SSY) dye from real wastewater at neutral pH conditions. The as-synthesized SnO2 and MgO composite was investigated using XRD, SEM, EDX, TEM, XPS, BET, and zeta potential. The experimental study of the SSY removal using SnO2/MgO composite was performed at different conditions, such as pH, stirring time, dose, and temperature. More than 99% of 10 mg/L SSY was effectively adsorbed from aqueous solution using 40 mg of SnO2/MgO composite at pH 7 and a stirring time of 60 min. The SSY adsorption behavior was well fitted by pseudo-second order and the Langmuir model, indicating that the SSY was chemisorbed to the composite-active sites as a monolayer. On the other hand, photocatalytic degradation process exhibited better results in terms of speed of removal and used quantity of photocatalyst, where 20 mg of SnO2/MgO composite can be used to remove > 99% of SSY dye within 30 min. Mechanism of SSY adsorption and photocatalytic degradation was discussed. In addition, elution experiments demonstrated that the SnO2/MgO composite as an SSY adsorbent could be reused for nine cycles without considerable reduction in the SSY adsorption efficiency. Therefore, this work exhibited that the mesoporous SnO2/MgO composite can be considered an effective adsorbent/photocatalyst to remove SSY dye from real industrial effluent water at neutral pH conditions.

Exploring the potential of quercetin/aspirin-loaded chitosan nanoparticles coated with Eudragit L100 in the treatment of induced-colorectal cancer in rats.

Growing evidence suggests quercetin and aspirin may have anticancer properties, notably in the case of colorectal cancer. The goal of this study was to create Pluronic F127 and polyethylene glycol4000 solid dispersion-loaded chitosan nanoparticles for colonic quercetin and aspirin delivery. In 1:1 polymeric stoichiometric ratio, solubility and complex formation were verified. Solid dispersion-loaded chitosan nanoparticles with a diameter of 244.45 ± 8.5 nm, a surface charge of 34.1 ± 3.3 mV, and encapsulation effectiveness of 76.3 ± 4.3% were generated under ideal conditions. In some cases, coating with Eudragit L100 resulted in a decrease in zeta potential and an increase in particle size. The coated formulation released the actives in a pH-dependent manner, considering their physicochemical features. Surprisingly, when compared to the actives' suspension and uncoated formulation, the coated formulation had greater anti-inflammatory efficacy, with a substantial reduction of PGE2 and IL-8 production in colonic tissues (16.9 ± 7.9 ng/g tissue and 134.9 ± 10.1 pg/g tissue, respectively). It also reversed most of the dimethyl hydrazine-induced histological alterations in the colon. It also demonstrated a greater reduction in TNF expression in colonic tissues. As a result, Eudragit L100-coated QT/AS-loaded chitosan nanoparticles are suggested to provide a potential platform for colonic delivery of quercetin and aspirin.

LC-MS/MS approach for simultaneous assessment of hyoscine N-butyl bromide and ketoprofen in biological fluids and pharmaceuticals.

The mixture of hyoscine N-butyl bromide (HBB) and ketoprofen (KTP) is commonly used for the handling of abdominal spasms and pain relief. There are two challenges that restrict the simultaneous assessment of HBB and KTP in biological fluids and pharmaceuticals. The first issue is the difficulty of elution of HBB and the second one is the presence of KTP as a racemic mixture in all pharmaceutical formulations, which obscures its appearance as a single peak. An ultrasensitive and highly efficient liquid chromatography-mass/mass spectrometric (LC-MS/MS) method is designed and validated for the first concurrent assessment of HBB and KTP in spiked human serum and urine, and pharmaceutical formulations. The estimated linearity ranges for HBB and KTP were respectively, 0.5-500 and 0.05-500 ng/ml, with excellent correlation coefficients. Validation results showed that the value of relative standard deviations were <2% for HBB and KTP. The mean extraction recoveries for HBB and KTP were, respectively, 91.04 and 97.83% in Spasmofen® ampoules; 95.89 and 97.00% in spiked serum; and 97.31 and 95.63% in spiked urine. The presented innovative chromatographic approach was utilized for the measurement of trace amounts of coexisting pharmaceuticals in pharmacokinetics studies and routine therapeutic medication monitoring.

A Novel P@SiO2 Nano-Composite as Effective Adsorbent to Remove Methylene Blue Dye from Aqueous Media.

This work aims to prepare a novel phosphate-embedded silica nanoparticles (P@SiO2) nanocomposite as an effective adsorbent through a hydrothermal route. Firstly, a mixed solution of sodium silicate and sodium phosphate was passed through a strong acidic resin to convert it into hydrogen form. After that, the resultant solution was hydrothermally treated to yield P@SiO2 nanocomposite. Using kinetic studies, methylene blue (MB) dye was selected to study the removal behavior of the P@SiO2 nanocomposite. The obtained composite was characterized using several advanced techniques. The experimental results showed rapid kinetic adsorption where the equilibrium was reached within 100 s, and the pseudo-second-order fitted well with experimental data. Moreover, according to Langmuir, one gram of P@SiO2 nanocomposite can remove 76.92 mg of the methylene blue dye. The thermodynamic studies showed that the adsorption process was spontaneous, exothermic, and ordered at the solid/solution interface. Finally, the results indicated that the presence of NaCl did not impact the adsorption behavior of MB dye. Due to the significant efficiency and promising properties of the prepared P@SiO2 nanocomposite, it could be used as an effective adsorbent material to remove various cationic forms of pollutants from aqueous solutions in future works.

Chitosan-Functionalized-Graphene Oxide (GO@CS) Beads as an Effective Adsorbent to Remove Cationic Dye from Wastewater.

In this study, the preparation of graphene oxide@chitosan (GO@CS) composite beads was investigated via continuous dropping techniques to remove methylene blue (MB)-dye from an aqueous media. The prepared beads were characterized using various techniques before and after the adsorption of MB. The experimental results showed that the adsorption processes fit the kinetic pseudo-second-order and Langmuir isotherm models. Moreover, the GO@CS beads achieve maximum adsorption capacities of 23.26 mg g-1, which was comparable with other adsorbents in the literature. An important advantage of our adsorbent is that the GO@CS can remove 82.1% of the real sample color within 135 min.

High-Pressure Metal-Free Catalyzed One-Pot Two-Component Synthetic Approach for New 5-Arylazopyrazolo[3,4-b]Pyridine Derivatives.

An appropriate and efficient Q-tube-assisted ammonium acetate-mediated protocol for the assembly of the hitherto unreported 5-arylazopyrazolo[3,4-b]pyridines was demonstrated. This methodology comprises the cyclocondensation reaction of 5-amino-2-phenyl-4H-pyrazol-3-one with an assortment of arylhydrazonals in an NH4OAc/AcOH buffer solution operating a Q-tube reactor. This versatile protocol exhibited several outstanding merits: easy work-up, mild conditions, scalability, broad substrate scope, safety (the Q-tube kit is simply for pressing and sealing), and a high atom economy. Consequently, performing such reactions under elevated pressures and utilizing the Q-tube reactor seemed preferable for achieving the required products in comparison to the conventional conditions. Diverse spectroscopic methods and X-ray single-crystal techniques were applied to confirm the proposed structure of the targeted compounds.

Cross-Linked Chitosan/Gelatin Beads Loaded with Chlorella vulgaris Microalgae/Zinc Oxide Nanoparticles for Adsorbing Carcinogenic Bisphenol-A Pollutant from Water.

Water polluted by phenolic compounds is a global threat to health and the environment; accordingly, we prepared a green novel sorbent biological system from a chitosan (CS), gelatin (GT), and Chlorella vulgaris freshwater microalgae (m-Alg) composite impregnated with zinc oxide nanoparticles (ZnO-NPs) for the remediation of bisphenol-A (BPA) from water. C. vulgaris was selected to be one of the constituents of the prepared composite because of its high capability in phytoremediation. The morphology and the structure of CS/GT*m-Alg/ZnO beads were characterized by SEM, FTIR, XRD, and TGA. Different monitoring experimental conditions, such as contact time, pH, BPA concentration, and sorbent dosage, were optimized. The optimum conditions for the adsorption process showed outstanding removal efficiency toward BPA at pH 4.0, contact time 40.0 min, and 40.0 mg L-1 BPA initial concentration. Langmuir, Freundlich, and Temkin isotherm models have been studied for adsorption equilibrium, and the best fit is described by the Langmuir adsorption isotherm. The adsorption kinetics has been studied using pseudo-first-order (PFO), pseudo-second-order (PSO), Elovich, and intraparticle diffusion (IPD) models. The pseudo-second-order kinetic model shows the optimum experimental fit. The monolayer adsorption capacity of the prepared CS/GT*m-Alg/ZnO for BPA was determined to be 38.24 mg g-1. The prepared CS/GT*m-Alg/ZnO beads show advantageous properties, such as their high surface area, high adsorption capacity, reusability, and cost-effectiveness.

Cu-Doped 1D Hydroxyapatite as a Highly Active Catalyst for the Removal of 4-Nitrophenol and Dyes from Water.

Metallic copper nanoparticle (Cu NP)-doped 1D hydroxyapatite was synthesized using a simple chemical reduction method. To describe the structure and composition of the Cu/HAP nanocomposites, physicochemical techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, inductively coupled plasma, N2 adsorption-desorption, X-ray photoelectron spectroscopy, and high-resolution transmission electron microscopy were used. The TEM scan of the Cu/HAP nanocomposite revealed a rod-like shape with 308 nm length and 117 nm width on average. The catalytic activity of Cu/HAP nanocomposites for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 has been thoroughly investigated. The 0.7% Cu/HAP nanocomposite was shown to have superior catalytic activity than the other nanocomposites, converting 4-NP to 4-AP in ∼1 min with good recyclability. Moreover, this nanocomposite showed excellent catalytic performance in the organic dye reduction such as Congo red and acriflavine hydrochloride dyes. The high dispersion of Cu NPs on HAP support, the high specific surface area, and the small Cu particles contributed to its remarkable catalytic performance.

Facile Conversion of the Quinone-Semicarbazone Chromophore of Naftazone into a Fluorescent Quinol-Semicarbazide: Kinetic Study and Analysis of Naftazone in Pharmaceuticals and Human Serum.

Naftazone is a quinone-semi carbazone drug that possesses a strong orange color, and hence it was usually analyzed colorimetrically or by HPLC-UV. However, these methods are not sensitive enough to determine naftazone in biological samples. Naftazone lacks intrinsic fluorescence and does not possess easily derivatizable functional groups. In this contribution, we introduced the first spectrofluorimetric method for naftazone assay through reduction-elicited fluorogenic derivatization through the reduction of its quinone-semicarbazone moiety to the corresponding quinol-semicarbazide derivative by potassium borohydride as a reduction probe. The solvent-dependent fluorescence of the reaction product was studied in various protic and aprotic solvents. Eventually, the fluorescence of the reduced naftazone was measured in 2-propanol at λemission of 350 nm after excitation at λecxitation of 295 nm. The relative fluorescence intensity was linearly correlated to the drug concentration (r = 0.9995) from 10.0 to 500 ng/mL with high sensitivity, where the lower detection limit was 2.9 ng/mL. Hence, the method was effectively applied for naftazone tablets quality control with a mean %recovery of 100.3 ± 1.5, and the results agreed with those of the comparison HPLC-UV method. Furthermore, a new salting-out assisted liquid-liquid extraction (SALLE) method was established for naftazone extraction from human serum, followed by its determination using the developed reduction-based fluorogenic method. The developed SALLE method showed excellent recovery for naftazone from human serum (92.3-106.5%) with good precision (RSD ≤ 6.8%). Additionally, the reaction of naftazone with potassium borohydride was kinetically monitored, and it was found to follow pseudo-first-order kinetics with an activation energy of 43.8 kcal/mol. The developed method's greenness was approved using three green analytical chemistry metrics.

Oil tea shell synthesized biochar adsorptive utilization for the nitrate removal from aqueous media.

Various reported methods are devoted to nitrate removal from water over the years. However, recently researchers are focusing on developing the materials that offer bio-based, non-toxic, inexpensive and yet an efficient solution for water treatment. In this study, removal of nitrates from water was carried out using oil tea shells (OTS) as a biosorbent. OTS powder was impregnated with ZnCl2 and biochar was prepared which was further treated with Cetyltrimethylammonium bromide (CTAB), a cationic surfactant. Both the Langmuir and the Freundlich models were satisfied by the nitrate adsorption of OTS biochar. The adsorption capacity was measured at 15.6 mg/g when the circumstances were at their best. The pseudo-second-order model provided an accurate description of the kinetic data that were collected from batch trials. The adsorption yield goes up when by usage of more adsorbent, but it goes down when adsorption start with a higher concentration of nitrate. The strong basis of analytical equipments were used to characterize the OTS biosorbent. According to the findings of the research, surface-modified OTS biochar is an effective material for the removal of nitrate from aqueous solutions. This means that it has the potential to be utilized in water treatment as an adsorbent that is both inexpensive and kind to the natural environment. Removal of heavy metals and other organic pollutants, both from groundwater and wastewater using OTS biochar seems like a promising and interesting area of study.

Catalytic and Multicomponent Reactions for Green Synthesis of Some Pyrazolone Compounds and Evaluation as Antimicrobial Agents.

A green synthetic approach and facile method was developed to produce pyrazole compounds (6a-d) by the reaction of ethyl acetoacetate (1), hydrazines (2a-d), and catalytic imidazole (3) in aqueous media. 4-Dicyanomethylene-2-pyrazoline-5-one derivatives (14a-d) were synthesized through the reaction of 2-pyrazoline-5-one derivatives (6a-d) with tetracyanoethylene (TCE) (7) by using catalytic imidazole (3) in an aqueous medium. Moreover, the 4-dicyanomethylene derivative (16) was obtained via treatment of 1-phenyl-3,5-pyrazolidinedione (15) with TCE (7). The spiropyrazoleoxirane derivatives (18 and 20) were prepared by treating the precursor 4-dicyanomethylene-2-pyrazoline-5-one derivative (14b) with hydrogen peroxide in various polar solvents under alkaline conditions. The spiropyrazole oxirane derivative (18) was used as a precursor for the design of functionalized pyrazolone derivatives (24 and 27a, b). The chemical structure of the novel designed derivatives was ascertained based on elemental analyses, mp, thin-layer chromatography, and spectral analyses. Furthermore, some of the synthesized derivatives were examined against different pathogenic bacterial and fungal strains. Their results demonstrated that some of them revealed notable antimicrobial activities.

Effective adsorption of cadmium and lead using SO3H-functionalized Zr-MOFs in aqueous medium.

Cadmium (Cd) and Lead (Pb) from industrial wastewater can bioaccumulate in the living organisms of water bodies, posing serious threats to human health. Therefore, efficient remediation of heavy metal ions of Cd (II) and Pb (II) in aqueous media is necessary for public health and environmental sustainability. In the present study, water stable Zirconium (Zr) based metal organic frameworks (MOFs) with SO3H functionalization were synthesized by solvothermal method and used first time for the adsorption of Cd (II) and Pb (II). Synthesis of UiO-66-SO3H, nano-sized (<100 nm) MOFs, was confirmed by FTIR, XRD, FESEM and BET. Effects of contact time, pH and temperature were investigated for adsorption of Cd (II) and Pb (II) onto SO3H-functionalized Zr-MOFs. The UiO-66-SO3H displayed notable rejections of 97% and 88% towards Cd (II) and Pb (II), respectively, after 160 min at 25 °C and pH (6) with an initial concentration of 1000 mg/L. Adsorption capacities of Cd (II) and Pb (II) were achieved as 194.9154 (mg/g) and 176.6879 (mg/g), respectively, at an initial concentration of 1000 mg/L. The Pseudo second-order kinetic model fitted well with linear regression (R2) of value 1. The mechanism was confirmed mainly as a chemisorption and coordination interaction between sulfone group (-SO3H) and metal ions Cd (IIa) and Pb (II). These results may support effective adsorption and can be studied further to enrich and recycle other heavy metals from wastewater.

A comparative study of Cu-anchored 0D and 1D ZnO nanostructures for the reduction of organic pollutants in water.

In this work, Cu NPs were loaded at a fixed percentage (5 wt%) on 1D, (1D + 0D) and 0D ZnO nanostructures to investigate the effect of the support morphology on the reduction of organic pollutants in water. The synthesized materials were characterized by high-resolution transmission electron microscopy (HR-TEM), ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), N2 adsorption-desorption and X-ray photoelectron spectroscopy (XPS). The results reveal that the loading of Cu NPs decreases the optical band gap, and a slight change in the crystallite sizes increases the specific surface area value of the nanocomposites. The TEM images reveal that 1D ZnO has an average width of 44.7 nm and an average length of 211 nm, while 0D ZnO has an average diameter of 54.5 nm. The HR-TEM and XPS data confirm the loading of metallic Cu NPs on the surface of the ZnO nanostructures. The pure ZnO and nanocomposites were tested for 4-nitrophenol (4-NP) reduction in the presence of NaBH4 at room temperature. The obtained results show that pure ZnO nanostructures have no catalytic performance, while the nanocomposites showed good catalytic activities. The catalytic reduction efficiency of 4-NP was found to follow the order of Cu/0DZnO > Cu/(1D + 0D)ZnO > Cu/1DZnO. The complete reduction of 4-NP has been observed to be achievable within 60 s using the Cu/0DZnO nanocomposite, with a k app value of 8.42 min-1 and good recyclability of up to five cycles. This nanocomposite was then applied in the reduction of organic dyes in water; it was found that the reduction rate constants for the methylene blue, Congo red, and acriflavine hydrochloride dyes were 1.4 min-1, 1.2 min-1, and 3.81 min-1, respectively. The high catalytic performance of this nanocomposite may be due to the small particle size, high specific surface area, and the high dispersion of Cu NPs on the surface of ZnO.

Kinetics and Mechanism Studies of Oxidation of Dibromothymolsulfonphthalein Toxic Dye by Potassium Permanganate in Neutral Media with the Synthesis of 2-Bromo-6-isopropyl-3-methyl-cyclohexa-2,5-dienone.

The oxidation of 3',3″-dibromothymolsulfonphthalein (DBTS) in neutral medium by potassium permanganate multi-equivalent oxidant has been studied spectrophotometrically. Pseudo-first-order plots showed inverted S-shape throughout the entire course of the reaction. The initial rates were found to be relatively fast in the early stages, followed by a decrease in the oxidation rates over longer time periods in the slow stage. Under pseudo-first-order conditions where [DBTS] ≫ 10 [MnO4 -], the experimental results showed a first-order dependence in [MnO4 -] and fractional-first-order kinetics in the [DBTS] concentration. The formation of 1:1 coordination intermediate complex prior to the rate-determining step was revealed kinetically. In addition, the intermediate species involving complexes of Mn(V) coordination has been detected. The oxidation product of DBTS was identified by Fourier transform infrared spectroscopy, ultraviolet-visible spectrophotometry, and gas chromatography-mass analysis. The obtained results indicated the formation of 2-bromo-6-isopropyl-3-methyl-cyclohexa-2,5-dienone as a derivative oxidation of DBTS.

Impact of highly phospholipid-containing lipid nanocarriers on oral bioavailability and pharmacodynamics performance of genistein.

Oxidative stress is a leading cause of different diseases. Genistein is a valuable bioflavonoid possessing antioxidant and anti-inflammatory activity but unfortunately, it suffers from low aqueous solubility, extremely poor bioavailability and first pass effect when used in its pure state. The aim of this work was to formulate and characterize genistein-loaded highly phospholipid-containing lipid nanocarriers to improve oral bioavailability and pharmacodynamic performance. Lipid nanocarriers were prepared by the emulsification/sonication technique. The influence of phospholipid percentage (1%-10%) on physicochemical properties, drug release and stability was investigated. The particle size, zeta potential and EE% were in ranges from 211.9 ± 21.6 to 342.3 ± 7.9 nm, -11.6 ± 1.7 to -19.4 ± 3.1 mV and 78.5 ± 4.7% to 92.2 ± 1.9%, respectively. Drug release was less predominant in the case of SLN formulations when compared to corresponding NLC formulations. High phospholipid percentage produced less stable formulations in terms of particle size growth, gelation and heterogeneous particle distributions. DSC, FT-IR and XRD tools revealed that genistein has existed in an amorphous form in NLC4. The bioavailability of NLC4 was approximately 2.6-fold greater than that of conventional suspension. Additionally, lipid peroxidation in liver homogenate and histopathological alterations in liver and kidney sections were particularly improved, providing a promising strategy for oral administration of genistein.

Investigation of Chemical Compositions and Biological Activities of Mentha suaveolens L. from Saudi Arabia.

Mentha is an aromatic plant used since antiquity for its pharmaceutical virtues. The climate of Saudi Arabia favors the growth of aromatic plants including Mentha suaveolens L. The aim of this study is to analyze the volatile oils of different parts of fresh and dried Mentha suaveolens L. grown in Saudi Arabia (Aljouf area) using Gas Chromatography/Mass Spectrometry (GC/MS) and Gas Chromatography Flame Ionization Detector (GC/FID) techniques, to recognize the effect of drying on chemical composition, then to evaluate the antioxidant and antifungal activities of different extracts. In total, 118 compounds were identified via GC/MS and GC/FID, in which carvone is the main volatile constituent (stems, leaves, whole plant 45-64%). This investigation deduces that Mentha belonged to the carvone chemotype. Then, the analysis of non-volatile constituents of fresh and dried Mentha was performed by HPLC. The main phenolic compound of fresh and dried Mentha for different parts was rosmarinic acid (ranging from 28,002.5 to 6558 µg/g). The ethanolic extract of fresh stem showed the highest antifungal activity (53% inhibition) compared with miconazole (60% inhibition) but the ethanoic extract of dry stem showed no activity. Additionally, all ethanolic extracts, whether for fresh or dry Mentha, have antioxidant activity more than 90% while the antioxidant activity of whole plant volatile oil is equal to 53.33%. This research shows that M. suaveolens L. could be applied to manufacture natural antioxidants, antifungal, and flavoring agents.

A novel spectrofluorimetric method based on a reaction with an azoisoxazoles-benzenesulfonamide derivative for determination of uranium (VI) ions in water samples.

Selective fluorometric detection and determination of uranium ions is provided here using a novel fluorescent reagent, namely (E)-4-([4-hydroxynaphthalen-1-yl]diazenyl)-N-(5-methyleisoxazol-3-yl) benzenesulfonamide (UVI reagent). The UVI reagent offers a selective fluorescence enhancement behaviour at emission wavelength = 557 nm. The parameters affecting fluorometric detection of uranium ions, such as the pH, solvent type, ligand concentration, interaction time, and interfering ions, were investigated and adjusted. The proposed UVI reagent can detect and determine uranium ions even at low concentrations, for which the obtained limit of detection was 0.1 ppm. Additionally, this proposed determination protocol was successfully used to detect, monitor, and determine uranium ions in actual water samples.

Environmental impact of the reported chromatographic methods for the determination of the first FDA-Approved therapy for COVID-19 Patients, Remdesivir: A comparative study.

Remdesivir (REM) is considered the first therapeutic option approved by US Food and Drug Administration (FDA) for clinical care in case of hospitalized patients suffering in COVID-19 epidemic. In the presented multilateral comparative search, four eco friendlessness approaches -National Environmental Methods Index (NEMI), Eco-Scale Assessment (ESA), Green Analytical Procedure Index (GAPI), and Analytical Greenness metric (AGREE) are tested to assess 16 analytical chromatographic procedures reported for the analysis of the commonly used antiviral drug; Remdesivir (REM). The values of testing more than one approach when estimating the eco-friendly characters for analytical methods are illustrated in this study. On the light of the outcomes, ESA and AGREE approaches are recommended as they are easily applied and digitally presented. Furthermore, GAPI is also a reliable tool in terms of comprehensiveness for the whole analytical procedures, from sampling till the final assessment. NEMI is the easiest and fastest greenness evaluation tool; however, the information it provides is particularly of limited scope and sometimes inaccurate. To ensure greenness of chromatographic analytical methods, there must be clear planning beforehand, to reduce chemical hazards sent to environment. Additionally, it is highly recommended in method validation protocols to consider the greenness of a given analytical procedure before releasing to routine use. The LC-MS/MS analysis for the active metabolite of REM (Nuc) reported by Avataneo et al. and Du et al. proved to be the best bio-analytical methods regarding the environmental aspects depending on the GAPI and AGREE tools. However, the HPLC method for REM analysis in intravenous solution reported by Jitta et al. proved to be the greenest analytical method for determination of REM in the pharmaceutical dosage forms according to the ESA, GAPI, and AGREE tools.