Epidemiology and outcome analysis of 3030 burn patients with an ICD-10 approach.

The present study aims to document the epidemiologic features and outcomes of burn injuries in Southeastern Iran based on International Statistical Classification of Diseases and Related Health Problems, 10th Revision (ICD-10) guidelines. This retrospective cross-sectional study was carried out at Khatam-Al-Anbiya Hospital. Patient demographics, including burn injury data and outcome data were collected from medical records and analyzed through descriptive and analytical statistics using SPSS software. A total of 3,030 burn patients were included in this study. A total of 55% of the subjects were males. The largest age group included patients aged 15-44 (61%). The majority of burns were caused by flame (70.5%), and most of them were third-degree burns (73%). Mean affected total body surface area (TBSA) was 43.98%±30.75% in all subjects and 80.85%±21.41% in the deceased individuals. Most of the burns were accidental (66.2%), and 37% of them occurred in winter. Mean hospital stay was 4.49±4.67 days (within the range of 1-113 days). A quarter of all patients admitted to the hospital died (24.9%). The number of admitted patients, mean length of stay (LOS), and the mortality rate showed a decreasing trend from 2007 to 2016. In contrast, the total mortality rate was high. The significant predictors of mortality included being female, flame burns, longer LOS, a larger TBSA, burns of higher degrees, as well as burn complications. The documentation of burn data, based on ICD-10 directives, standardizes findings from burn injury analyses and leads to the comparability of data at different national and international levels.

Cette étude rapporte l'épidémiologie et le devenir des patients brûlés dans le sud- est iranien, en utilisant la CIM 10. Elle a été conduite dans l'hôpital Khatam-Al-Anbiya. Les données démographiques ainsi que celles concernant la brûlure et le devenir des patients ont été analysées, en utilisant SSPS. Parmi les 3 030 patients, 55% étaient des hommes. La tranche d'âge la plus représentée (61%) était 15­44 ans. La majorité des brûlures, très souvent (73%) de 3ème degré étaient dues à une flamme (70,5%). La surface brûlée était globalement de 43,98+/30,75% (80,85+/-21,41% chez les patients décédés). Les brûlures étaient le plus souvent accidentelles (66,2% des cas), 37% d'entre elles survenant en hiver. La durée d'hospitalisation était de 4,49+/-4,67 jours (1-113), le quart (24,9%) des patients étant décédés. On observe une baisse du nombre de patients, de la durée de séjour et de la mortalité entre 2007 et 2016, cette dernière restant élevée. Les variables corrélées à la mortalité sont le sexe féminin, la brûlure par flamme, l'augmentation de la surface brûlée et de la durée de séjour, la profondeur de la brûlure et l'existence de complications. L'utilisation de la CIM 10 permet une standardisation des études épidémiologiques, ce qui les rend comparables entre elles.

Genotype characterization of livestock and human cystic echinococcosis in Mazandaran province, Iran.

Echinococcus granulosus is a helminth from the family Taeniidae, which causes cystic echinococcosis (CE) in humans and diverse livestock around the world. The identification of existing genotypes in different regions is a major step towards the prevention and establishment of control programmes for the disease. This study aimed to detect CE genotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the internal transcribed spacer-1 (ITS1) gene and sequencing of the cytochrome c oxidase subunit 1 (Cox1) gene in isolates from the central part of Mazandaran province, northern Iran. Forty isolates were collected from sheep, 17 from cattle and 6 from human formalin-fixed paraffin-embedded tissues (FFPE). The ITS1 and Cox1 genes were successfully amplified by PCR in 41 and 42 samples, respectively. PCR-RFLP and sequencing showed that all isolates had the G1-G3 genotypes in this study. Out of 31 isolates subjected to sequencing for the Cox1 gene, 80.7% had the G1 genotype. G2 (16.1%) and G3 (3.2%) genotypes were observed in five sheep and one cattle samples, respectively. Five human isolates were also sequenced for the ITS1 gene, which showed that all samples belonged to the G1 genotype. Ten haplotypes were determined among the isolates by alignment analysis of the Cox1 gene. In summary, this study demonstrated that G1 was the dominant genotype circulating between humans and livestock in the studied region. Furthermore, high genotypic diversity among the CE isolates was observed.

Seroprevalence and molecular detection of Toxoplasma gondii in young healthy blood donors in Northern Iran.

T. gondii is a life-threatening infection in immunocompromised patients which may be transmitted through blood transfusion. The present study aimed to evaluate the seroprevalence and molecular detection of T. gondii infection and the associated risk factors among young healthy blood donors in the central part of Mazandaran province, northern Iran. Blood samples were taken from 500 participants and the serum was separated. All serum samples were tested for the presence of anti-T. gondii antibodies (IgG) and then all positive samples were evaluated for IgM antibodies using commercial ELISA kits. All IgM positive samples and 66 randomly selected IgG positive samples were further tested by PCR of the REP-529 gene. Anti-Toxoplasma antibodies (IgG) avidity test was performed for 142 IgG positive samples which were randomly selected. In the current study, anti-T. gondii antibodies (IgG) and (IgM) were found in 316 (63.2%) and 3 (0.95 %) participants, respectively. Seropositivity rate of Toxoplasma was higher among blood donors living in rural areas (P=0.000) and those with a history of soil and animal contact (P<0.05). PCR of the REP-529 gene showed T. gondii DNA in 21 out of 66 samples. The REP-529 gene was not detected in IgM positive samples. Low avidity antibodies (IgG) was found in 23.2% of the IgG positive samples. In conclusions, this study found that the prevalence of toxoplasmosis among young healthy blood donors in north of Iran was high. To reduce the risk of parasite transmission, leukofilteration method are recommended for donated blood used for immunosuppressed patients.

Seroprevalence and molecular detection of Toxoplasma gondii in young healthy blood donors in Northern Iran

@#T. gondii is a life-threatening infection in immunocompromised patients which may be transmitted through blood transfusion. The present study aimed to evaluate the seroprevalence and molecular detection of T. gondii infection and the associated risk factors among young healthy blood donors in the central part of Mazandaran province, northern Iran. Blood samples were taken from 500 participants and the serum was separated. All serum samples were tested for the presence of anti-T. gondii antibodies (IgG) and then all positive samples were evaluated for IgM antibodies using commercial ELISA kits. All IgM positive samples and 66 randomly selected IgG positive samples were further tested by PCR of the REP-529 gene. Anti-Toxoplasma antibodies (IgG) avidity test was performed for 142 IgG positive samples which were randomly selected. In the current study, anti-T. gondii antibodies (IgG) and (IgM) were found in 316 (63.2%) and 3 (0.95 %) participants, respectively. Seropositivity rate of Toxoplasma was higher among blood donors living in rural areas (P=0.000) and those with a history of soil and animal contact (P<0.05). PCR of the REP-529 gene showed T. gondii DNA in 21 out of 66 samples. The REP-529 gene was not detected in IgM positive samples. Low avidity antibodies (IgG) was found in 23.2% of the IgG positive samples. In conclusions, this study found that the prevalence of toxoplasmosis among young healthy blood donors in north of Iran was high. To reduce the risk of parasite transmission, leukofilteration method are recommended for donated blood used for immunosuppressed patients.