Encapsulation of Nepeta hormozganica and Nepeta dschuprensis essential oils in shrimp chitosan NPs: Enhanced antifungal activity.

This study investigated the encapsulation of Nepeta hormozganica (NHEO) and Nepeta Dschuprensis (NDEO) essential oils into chitosan nanoparticles (CSN) via a simple ionic gelation method with tripolyphosphate (TPP). Chitosan (CS) is prepared by demineralizing, deproteinizing, and deacetylating shrimp shells waste in high yield (70.2 %). SEM, TEM, FT-IR, TGA, and XRD techniques were employed to characterize the encapsulated essential oils ((NHEO-CSN) and (NDEO-CSN)). The prepared EOs-CSN and CSN are found with particle sizes of 100-150 nm and 400-500 nm, respectively, and regular distribution. The encapsulation efficiency of encapsulated Nepeta hormozganica and Dschuprensis essential oils were found to be 73.64 % and 75.91 %, respectively. The synthesized nanocapsules were evaluated for antifungal activity against Fusarium oxysporium, Sclerotinia sclerotiorum, Pythium aphanidermatum, Alternaria alternata, Rhizactonia Solani, and Botrytis cinerea. Antifungal studies show that encapsulated essential oils increased antifungal efficiency by up to 100 %.

Chitosan nanocomposite incorporated Satureja kermanica essential oil and extract: Synthesis, characterization and antifungal assay.

The present study reports the design, synthesis, and characterization of nanoencapsulated Satureja kermanica essential oil/extract by chitosan biopolymer (SKEO-CSN)/(SKEX-CSN) for the antifungal efficacy against Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, and Pythium aphanidermatum. The prepared SKEO-CSN and SKEX-CSN were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction analysis (XRD). GC-Mass analysis was done to identify Satureja kermanica essential oil chemical compounds (SKEO). Thirty-five different components were detected from GC-MS analysis. Thymol (46.54 %), and Carvacrol (30.54 %) were demonstrated as major compounds. Antifungal studies showed that the SKEO-CSN and SKEX-CSN formulation effectively inhibit fungal growth more than free SKEO and SKEX. According to the results, SKEO-CSN and SKEX-CSN provide a wide range of promising antifungal effects and can be applied as an efficient green strategy to protect plants from fungus infections.

Physicochemical changes of barberry juice concentrated by liquid desiccant-assisted solar system and conventional methods during the evaporation process.

The preservation of the quality characteristics of fruit juices during the processing is one of the major challenges in the fruit juice industries. The high costs of fossil fuels and their environmental pollution increased the need to use of the renewable energies. In the present study, some quality characteristics of barberry juice were investigated during concentrating by the methods of liquid desiccant-assisted solar juice concentration system (LD-ASJCS) at the air flow rates of 0.006-0.014 kg/s, rotary vacuum evaporation system (RVES), and evaporation at atmospheric pressure (EAP). Although evaporation rate in LD-ASJCS increased by 25% with increasing of air flow rate from 0.006 to 0.014 kg/s, but it had negative effects on some quality properties of concentrated juices. The anthocyanins retention in the concentrated juices with different air flow rates of LD-ASJCS method were 16-28%. Reduction of the air flow rate resulted in a lower browning index and less variation of color indices (L*, a*, and b*). The a* values, browning index, and sensory attributes of the concentrated juices in the LD-ASJCS at the air flow rate of 0.006 kg/s were comparable to the concentrated juices by the RVES method. Although, RVES method compared to EAP and LD-ASJCS methods better improved the quality of barberry juice, but the LD-ASJCS method can be considered in fruit juice industries because of maintaining quality and sensory attributes, saving energy, and reducing environmental pollution. The mathematical modelling of physicochemical changes of LD-ASJCS concentrated juice showed that these variations are fitted with first-order and zero-order models.

Studies of Microbiota Dynamics Reveals Association of "Candidatus Liberibacter Asiaticus" Infection with Citrus (Citrus sinensis) Decline in South of Iran.

Citrus Decline Disease was recently reported to affect several citrus species in Iran when grafted on a local rootstock variety, Bakraee. Preliminary studies found "Candidatus Phytoplasma aurantifoliae" and "Candidatus Liberibacter asiaticus" as putative etiological agents, but were not ultimately able to determine which one, or if an association of both, were causing the disease. The current study has the aim of characterizing the microbiota of citrus plants that are either asymptomatic, showing early symptoms, or showing late symptoms through amplification of the V1­V3 region of 16S rRNA gene using an Illumina sequencer in order to (i) clarify the etiology of the disease, and (ii) describe the microbiota associated to different symptom stages. Our results suggest that liberibacter may be the main pathogen causing Citrus Decline Disease, but cannot rule out the possibility of phytoplasma being involved as well. The characterization of microbiota shows that the leaves show only two kinds of communities, either symptomatic or asymptomatic, while roots show clear distinction between early and late symptoms. These results could lead to the identification of bacteria that are related to successful plant defense response and, therefore, to immunity to the Citrus Decline Disease.