RESUMO
We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of polymorphism was conserved in all microsatellite markers. Cross-species amplification from birds of economic and ecological interest, such as chicken and turkey, could be an attractive approach to develop microsatellite markers and to use these to manage wild and captive populations of other galliforms, such as the grey partridge.
Assuntos
Repetições de Microssatélites/genética , Polimorfismo Genético , Alelos , Animais , Galinhas , Conservação dos Recursos Naturais , Ecologia , Marcadores Genéticos , Heterozigoto , Modelos Estatísticos , Especificidade da Espécie , PerusRESUMO
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
Assuntos
DNA de Helmintos/isolamento & purificação , Repetições de Microssatélites/genética , Reação em Cadeia da Polimerase/economia , Schistosoma mansoni/genética , Animais , Biomphalaria/parasitologia , Genótipo , Estágios do Ciclo de Vida , Camundongos , Reação em Cadeia da Polimerase/métodos , Reprodutibilidade dos Testes , Fatores de TempoRESUMO
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).