RESUMO
The atlC gene (1,485 bp), encoding an autolysin which binds fibronectin, and the ica operon, involved in biofilm formation, were isolated from the chromosome of an infectious isolate of Staphylococcus caprae and sequenced. AtlC (155 kDa) is similar to the staphylococcal autolysins Atl, AtlE, Aas (48 to 72% amino acid identity) and contains a putative signal peptide of 29 amino acids and two enzymatic centers (N-acetylmuramoyl-L-alanine amidase and endo-beta-N-acetylglucosaminidase) interconnected by three imperfect fibronectin-binding repeats. The glycine-tryptophan (GW) motif found in the central and end part of each repeat may serve for cell surface anchoring of AtlC as they do in Listeria monocytogenes. The S. caprae ica operon contains four genes closely related to S. epidermidis and S. aureus icaA, icaB, icaC, and icaD genes (> or = 68% similarity) and is preceded by a gene similar to icaR (> or =70% similarity). The polypeptides deduced from the S. caprae ica genes exhibit 67 to 88% amino acid identity to those of S. epidermidis and S. aureus ica genes. The ica operon and icaR gene were analyzed in 14 S. caprae strains from human specimens or goats' milk. Some of the strains produced biofilm, and others did not. All strains carry the ica operon and icaR of the same sizes and in the same relative positions, suggesting that the absence of biofilm formation is not related to the insertion of a mobile element such as an insertion sequence or a transposon.
Assuntos
Biofilmes , Fibronectinas/genética , Genes Bacterianos , N-Acetil-Muramil-L-Alanina Amidase/genética , Óperon , Staphylococcus/genética , Bacteriólise , Sítios de Ligação , Mapeamento Cromossômico , Staphylococcus/patogenicidade , Virulência/genéticaRESUMO
A variant of the vga(A) gene (1,575 bp), encoding an ATP-binding cassette protein conferring resistance to streptogramin A and related antibiotics, was cloned from the chromosome of a Staphylococcus aureus clinical isolate and sequenced. The sequence of the variant was similar to that of the vga(A) gene (83.2% identity). However, the G+C content of the variant (35.6%) was higher than that of vga(A) (29%) and there was no cross hybridization between vga(A) and the variant at high stringency (> or =60 degrees C), the highest temperature at which a signal was detected being 55 degrees C. Unlike previous reports for vga(A) and vga(B), the variant of vga(A) may be present in multiple copies in the genome. These copies are chromosomal in some isolates and both chromosomal and plasmid-borne in others. Nucleotide sequences hybridizing at 65 degrees C with the vga(A) variant were found in all the staphylococcal strains harboring plasmids carrying both vga(B) and vat(B), which also encode resistance to streptogramin A.
Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Staphylococcus aureus/genética , Virginiamicina/farmacologia , Alelos , Motivos de Aminoácidos , Proteínas de Bactérias/fisiologia , Clonagem Molecular , Primers do DNA , Sondas de DNA , DNA Bacteriano/análise , Resistência Microbiana a Medicamentos/genética , Humanos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Plasmídeos/genética , Reação em Cadeia da Polimerase , Staphylococcus aureus/efeitos dos fármacos , TemperaturaRESUMO
Fifty-four Staphylococcus epidermidis strains responsible for infections of joint prostheses and 23 strains isolated from skin flora were studied for markers of virulence, to discriminate invasive strains from normal flora. They were screened for binding to polystyrene and matrix proteins and for the presence of staphylococcal genes involved in adhesion. The ica operon involved in biofilm formation was the only marker discriminating between these 2 categories of strains.
Assuntos
Prótese Articular/microbiologia , Infecções Relacionadas à Prótese/microbiologia , Pele/microbiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus epidermidis/isolamento & purificação , Aderência Bacteriana , Biomarcadores/análise , Proteínas da Matriz Extracelular/fisiologia , Humanos , Poliestirenos/farmacologia , Staphylococcus epidermidis/classificação , Staphylococcus epidermidis/patogenicidadeRESUMO
A gene almost identical to satG was isolated from an Enterococcus faecium strain. This gene was transferred to a Staphylococcus aureus recipient strain where it conferred resistance to streptogramin A. satG was found to be widely distributed among E. faecium strains but not detected among staphylococci.
Assuntos
Acetiltransferases/genética , Antibacterianos/farmacologia , Enterococcus faecium/efeitos dos fármacos , Staphylococcus/efeitos dos fármacos , Virginiamicina/farmacologia , Sequência de Bases , Resistência Microbiana a Medicamentos , Enterococcus faecium/genética , Técnicas de Transferência de Genes , Dados de Sequência Molecular , Staphylococcus/genéticaRESUMO
Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of them, pIP680 (11.3 kb), carrying the three streptogramin-resistance genes was sequenced. The seven native plasmids had in common a 12.1-kb part cocarrying the three Sg(R) genes. Sequence analysis of pIP680 revealed that the simultaneous presence of these three genes has probably resulted from cointegration of two plasmids: (i) a pAMbeta1-like plasmid harboring vat-vgb and whose replication gene has been inactivated by an IS257 insertion and (ii) a functional vga plasmid whose replication is similar to that of two staphylococcal plasmids, pSX267 and pSK41.
Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Acetiltransferases/genética , Proteínas de Bactérias/genética , DNA Bacteriano , Hemeproteínas/genética , Plasmídeos , Staphylococcus/genética , Virginiamicina/farmacologia , Sequência de Bases , Resistência Microbiana a Medicamentos , Genes Bacterianos , Dados de Sequência Molecular , Staphylococcus/efeitos dos fármacosRESUMO
We isolated and sequenced a plasmid, named pIP1714 (4,978 bp), which specifies resistance to streptogramins A and B and the mixture of these compounds. pIP1714 was isolated from a Staphylococcus cohnii subsp. cohnii strain found in the environment of a hospital where pristinamycin was extensively used. Resistance to both compounds and related antibiotics is encoded by two novel, probably cotranscribed genes, (i) vatC, encoding a 212-amino-acid (aa) acetyltransferase that inactivates streptogramin A and that exhibits 58.2 to 69.8% aa identity with the Vat, VatB, and SatA proteins, and (ii) vgbB, encoding a 295-aa lactonase that inactivates streptogramin B and that shows 67% aa identity with the Vgb lactonase. pIP1714 includes a 2,985-bp fragment also found in two rolling-circle replication and mobilizable plasmids, pUB110 and pBC16, from gram-positive bacteria. In all three plasmids, the common fragment was delimited by two direct repeats of four nucleotides (GGGC) and included (i) putative genes closely related to repB, which encodes a replication protein, and to pre(mob), which encodes a protein required for conjugative mobilization and site-specific recombination, and (ii) sequences very similar to the double- and single-strand origins (dso, ssoU) and the recombination site, RSA. The antibiotic resistance genes repB and pre(mob) carried by each of these plasmids were found in the same transcriptional orientation.
Assuntos
Genes Bacterianos , Fatores R/genética , Staphylococcus/efeitos dos fármacos , Staphylococcus/genética , Virginiamicina/farmacologia , Acetiltransferases/genética , Acetiltransferases/metabolismo , Sequência de Bases , DNA Bacteriano , Resistência Microbiana a Medicamentos/genética , Sinergismo Farmacológico , Hidrolases/genética , Hidrolases/metabolismo , Dados de Sequência Molecular , Fatores R/isolamento & purificação , Alinhamento de SequênciaRESUMO
Streptogramin and related antibiotics are mixtures of two compounds, A and B (e.g. Dalfopristin and Quinupristin), particularly against Gram-positive bacteria. Staphylococci resistant to these mixtures are always resistant to the A compounds but are not necessarily resistant to the B compounds. Resistance to A compounds and to the mixtures is conferred by acetyltransferases or ATP-binding proteins via unknown mechanisms. Several genes encoding each of the two categories of protein have been characterized and regularly detected on plasmids. Genes encoding lactonases, which inactivate B compounds, have been occasionally detected on these plasmids. Staphylococci which harbour plasmids conferring resistance to A compounds should not be treated with the mixtures even if they appear susceptible in vitro. Indeed, susceptibility to the mixtures of staphylococci carrying resistance to A compounds has often been attributed to partial loss of the plasmids conferring this resistance. When staphylococci are constitutively resistant to B compounds, the in vitro activities of the mixtures should be evaluated, because they are better correlated than MICs with their efficacy in therapy.
RESUMO
In an Algerian hospital where pristinamycin (Pt) was extensively used for the treatment of chronic osteomyelitis and for prophylaxis in bone surgery, the prevalence of pristinamycin-resistant (PtR) staphylococci during a five-month period (20%) was higher than that among staphylococci isolated elsewhere in Algeria (4.5%). Analysis of 13 PtR staphylococci isolated in this hospital revealed a diversity of plasmids and genes conferring resistance to Pt and to related antibiotics. Most of the PtR staphylococci were unrelated: they belonged to either different taxa or types. Nevertheless, some of the unrelated staphylococci harboured structurally related plasmids carrying streptogramin resistance genes. Thus, these plasmids may have contributed to the dispersion of these genes.
Assuntos
Antibacterianos/uso terapêutico , Plasmídeos/genética , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus/genética , Virginiamicina/uso terapêutico , Argélia/epidemiologia , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Tipagem de Bacteriófagos , Osso e Ossos/cirurgia , Infecção Hospitalar , Primers do DNA/genética , DNA Bacteriano/análise , DNA Bacteriano/genética , Surtos de Doenças , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos , Humanos , Testes de Sensibilidade Microbiana , Hibridização de Ácido Nucleico , Osteomielite/tratamento farmacológico , Reação em Cadeia da Polimerase , Prevalência , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/genética , Staphylococcus/efeitos dos fármacos , Staphylococcus/patogenicidade , Virginiamicina/administração & dosagemRESUMO
The Staphylococcus aureus plasmid gene, vgaB, conferring resistance to streptogramin (SgA) and related compounds (PIIA, virginiamycin M, mikamycin A, synergistin A, Dalfopristin) was cloned and sequenced. This gene potentially encodes a 552-aa protein, VgaB, of 61,327 Da, which exhibits a significant similarity with the ATP-binding domains of numerous proteins. VgaB has two ATP-binding domains containing each of the A and the B motifs described by Walker et al. [Walker, J.E., Saraste, M., Runswick, M.J., Gay, N.J., 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J., 1, 945-951], but does not include TM hydrophobic domains. The 155-amino-acid sequence between the two ATP-binding domains of VgaB is richer in Glu than the rest of the protein. The vgaB gene was found in 21 of the 52 SgA(R) and independent wt staphylococci investigated. In each of the 21 staphylococci, vgaB was carried on a plasmid of 50-90 kb also harboring the vatB gene encoding an acetyltransferase inactivating SgA. In all plasmids, vgaB and vatB have the same relative positions.
Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Proteínas de Bactérias/genética , Genes Bacterianos , Staphylococcus aureus/genética , Virginiamicina/farmacologia , Transportadores de Cassetes de Ligação de ATP/isolamento & purificação , Sequência de Aminoácidos , Proteínas de Bactérias/isolamento & purificação , Sequência de Bases , Clonagem Molecular , Resistência Microbiana a Medicamentos , Dados de Sequência Molecular , Fenótipo , Plasmídeos/genética , Mapeamento por Restrição , Análise de Sequência de DNA , Staphylococcus aureus/química , Staphylococcus aureus/efeitos dos fármacosRESUMO
The levels of resistance to pristinamycin (Pt) and to its major constituents, pristinamycin IIA and IB (PIIA and PIB, respectively; classified as streptogramins A and B, respectively) were determined for 126 staphylococcal isolates. The results suggest tentative susceptibility breakpoints of < or = 2, < or = 8, and < or = 0.5 microgram/ml for PIIA, PIB, and Pt, respectively. Fifty-six isolates that were inhibited by > or = 4 micrograms of PIIA per ml were investigated for the presence of staphylococcal genes encoding resistance to PIIA (vga, vat, and vatB) and PIB (vgb). None of these genes was found in the 4 isolates inhibited by 4 micrograms of PIIA per ml or in 4 of the other 52 isolates tested. The remaining 48 isolates harbored plasmids carrying vatB and vga or combinations of genes (vga-vat-vgb or vga-vat). The absence of any known PIIA resistance gene from the four Staphylococcus aureus isolates inhibited by > or = 8 micrograms of PIIA per ml suggests that there is at least one PIIA resistance mechanism in staphylococci that has not yet been characterized.
Assuntos
Antibacterianos/farmacologia , Genes Bacterianos/genética , Staphylococcus/efeitos dos fármacos , Staphylococcus/genética , Virginiamicina/farmacologia , Western Blotting , Meios de Cultura , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , Resistência Microbiana a Medicamentos , Testes de Sensibilidade Microbiana , Hibridização de Ácido Nucleico , Plasmídeos/genética , Reação em Cadeia da PolimeraseRESUMO
A gene encoding an acetyltransferase inactivating streptogramin A (SgA) and structurally similar compounds was isolated from a staphylococcal plasmid and sequenced. This gene, designated vatB, potentially encodes a 212-amino-acid protein, VatB, of 23,320 Da with 47.4 and 58.4% amino acid identities with two other enzymes with the same activity, Vat and SatA, respectively, which are encoded by a staphylococcal plasmid and an enterococcal plasmid, respectively. The C-terminal parts of these three enzymes share significant homology with the C-terminal parts of 10 other acetyltransferases modifying various substrates. A pair of degenerate primers representing the conserved motifs shared by VatB, Vat, and SatA was designed to detect the three genes encoding these SgA acetyltransferases. Five of 12 clinical SgAr Staphylococcus aureus isolates tested carried neither these genes nor the gene vga, which confers resistance to SgA by a different mechanism, suggesting that another gene(s) and possibly another mechanism of resistance to SgA in staphylococci remains to be characterized.
Assuntos
Acetiltransferases/metabolismo , Antibacterianos/metabolismo , Bactérias Gram-Positivas/enzimologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/genética , Virginiamicina/metabolismo , Acetiltransferases/genética , Sequência de Aminoácidos , Antibacterianos/farmacologia , Sequência de Bases , Conjugação Genética , Meios de Cultura , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , DNA Bacteriano/metabolismo , Genes Bacterianos , Bactérias Gram-Positivas/efeitos dos fármacos , Immunoblotting , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Plasmídeos , Reação em Cadeia da Polimerase , Virginiamicina/farmacologiaRESUMO
The Staphylococcus aureus plasmids, pIP680 and pIP1156, which confer resistance to A-type compounds of virginiamycin-like antibiotics (Vml: streptogramin A, pristinamycin IIA, virginiamycin M) and to synergistic mixtures of the A and B compounds of Vml antibiotics, were shown to direct the modification of A-type compounds by acetylation. The vat gene, encoding the acetyltransferase modifying A-type compounds, was isolated from plasmid pIP680 and sequenced. This gene potentially encodes a 219-amino-acid (aa) protein, VAT, of 24 330 Da showing at least 38% aa identity with two chloramphenicol acetyltransferases encoded by cat genes isolated from Escherichia coli and Agrobacterium tumefaciens. Resistance to A-type compounds of Vml antibiotics conferred to S. aureus by vat was not expressed in E. coli, although a protein having a M(r) similar to that encoded by this gene was detected in E. coli minicells. The vat gene was detected by the polymerase chain reaction in two chromosomally located staphylococcal conjugative elements and in the conjugative plasmid, pIP1156, conferring resistance to A-type compounds.
Assuntos
Acetiltransferases/genética , Proteínas de Bactérias , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos , Fatores R/genética , Staphylococcus aureus/genética , Virginiamicina/farmacologia , Acetiltransferases/química , Sequência de Aminoácidos , Sequência de Bases , Cloranfenicol O-Acetiltransferase/genética , Clonagem Molecular , Contraindicações , DNA Bacteriano/análise , Dados de Sequência Molecular , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Staphylococcus aureus/enzimologiaRESUMO
DNA fragments, 450 bp in length, were amplified by polymerase chain reaction (PCR) from the thermonuclease gene (nuc) carried by seven epidemiologically independent Staphylococcus aureus isolates. Sequencing of the PCR products led us to characterize 210 bp strictly conserved. A 186 bp piece from within this conserved region was cloned into pUC18. The resulting recombinant plasmid, pIP1608, was used as a probe against the cellular DNA of 360 staphylococcal isolates belonging to 28 species. Only the 146 S. aureus isolates, including four which were not thermonuclease producers, had DNA that hybridized with pIP1608. Among the 214 non-S. aureus staphylococci, 55 exhibited a thermonuclease activity. For 32 of these, the enzymatic activity was inhibited by a commercially available polyclonal antiserum directed against the thermonuclease of an S. aureus strain. These results are in favour of the use of pIP1608 as a probe to specifically recognize S. aureus. Furthermore, we propose a method based on colony blot hybridization and potentially useful to enumerate S. aureus cells in biological samples.
Assuntos
Sondas de DNA , Genes Bacterianos , Nuclease do Micrococo/genética , Staphylococcus aureus/isolamento & purificação , Sequência de Bases , Clonagem Molecular , Primers do DNA , DNA Bacteriano/genética , Fluoresceína , Fluoresceínas , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Análise de Sequência de DNA , Especificidade da Espécie , Staphylococcus aureus/enzimologia , Staphylococcus aureus/genéticaRESUMO
The Staphylococcus aureus plasmid gene, vga, conferring resistance to A compounds of virginiamycin-like antibiotics (streptogramin A, pristinamycin II, virginiamycin M), and to synergistic mixtures of the A and B compounds of these antibiotics, was cloned and sequenced. This gene potentially encodes a 522-amino acid protein, VgA, of 60,115 Da which exhibits significant homology with the ATP-binding domains of numerous proteins. VgA has two ATP-binding domains, containing each the A and the B motifs, but does not contain long hydrophobic stretches that might represent potential membrane-spanning domains. Resistance to A compounds of virginiamycin-like antibiotics conferred to S. aureus by gene vga was not conferred to Escherichia coli, although a protein having an M(r) similar to that encoded by this gene was detected in E. coli minicells.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Trifosfato de Adenosina/metabolismo , Proteínas de Bactérias/genética , Plasmídeos/genética , Staphylococcus aureus/genética , Virginiamicina/farmacologia , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Clonagem Molecular , DNA Bacteriano , Resistência Microbiana a Medicamentos/genética , Dados de Sequência Molecular , Ligação Proteica , Mapeamento por RestriçãoRESUMO
The nucleotide sequence of a 1883 bp fragment isolated from a resistance plasmid harbored by a Staphylococcus aureus clinical isolate and carrying the gene, vgb, encoding a hydrolase inactivating the B components of virginiamycin family has been determined. The sequence contains one open reading frame which extends from the ATG codon at nt 641 to a TGA codon at nt 1537 and which potentially codes for a protein of 33.035 Da. This value is in agreement with the apparent size (33 kDa) of the protein observed, in minicell extracts. Inactivation of the B components of the virginiamycin antibiotics as well as resistance to these antibiotics were expressed in a virginiamycin sensitive mutant of Escherichia coli recipient containing the gene on a high copy number plasmid.
Assuntos
Genes Bacterianos , Genes , Hidrolases/genética , Fatores R , Staphylococcus aureus/genética , Sequência de Aminoácidos , Antibacterianos/metabolismo , Sequência de Bases , Dados de Sequência Molecular , Mapeamento por Restrição , Staphylococcus aureus/enzimologia , Virginiamicina/metabolismoRESUMO
Eleven Staphylococcus aureus clinical isolates were tested for transfer of resistance markers by transduction and filter mating. The resistance markers of six of the strains could be transferred only by transduction; however, the five remaining strains transferred their resistance both by transduction and filter mating. The resistance markers that were cotransferred in filter matings (transfer of resistance to penicillin and streptogramin A was accompanied, in each case, by the transfer of one or more markers, i.e., resistance to aminoglycosides, cadmium, or tetracycline, depending on the donor) were not cotransduced. The filter mating transfers were recA independent and were observed with both Staphylococcus aureus and Staphylococcus epidermidis recipients. Experiments to elucidate the mechanism of transfer by filter mating suggested that conjugation requiring cell-to-cell contact may have been involved. These transfers occurred in the absence of detectable plasmid DNA.
Assuntos
Antibacterianos/farmacologia , DNA Bacteriano/genética , Staphylococcus aureus/genética , Antibacterianos/metabolismo , Conjugação Genética , Resistência Microbiana a Medicamentos , Mutação , Plasmídeos , Fagos de Staphylococcus/genética , Staphylococcus aureus/efeitos dos fármacos , Transdução GenéticaRESUMO
Resistance to pristinamycin (or virginiamycin) was first encountered in Staphylococcus aureus strains in 1975. These strains are usually multiresistant, in particular to streptogramin A components (SgA), macrolides, lincosamides and streptogramin B components (ML SgB ). Results of molecular analysis of 16 such strains, recently isolated, suggests that SgA resistance is not encoded by plasmid genes. Curing and mixed culture experiments allowed us to dissociate SgA from SgB resistance genes. Conversely, in a previous study on other strains, the same two resistance genes were shown to be carried by a single plasmid and could not be dissociated. Since 1981, a new type of pristinamycin -resistant S. aureus strains has been isolated. These strains are resistant to SgA and lincosamides but susceptible to macrolides and SgB . Eight such strains from 3 parisian hospitals have been studied. In mixed culture experiments, SgA resistance and penicillinase genes always transferred jointly. In some instances, these two determinants also cotransferred with genes encoding lincomycin, lincomycin and clindamycin, and/or aminoglycosides resistance.