Removal of heavy oil from contaminated surfaces with a detergent formulation containing biosurfactants produced by Pseudomonas spp.

Industrial plants powered by heavy oil routinely experience problems with leaks in different parts of the system, such as during oil transport, the lubrication of equipment and mechanical failures. The surfactants, degreasing agents and solvents that make up detergents commonly used for cleaning grease-covered surfaces are synthetic, non-biodegradable and toxic, posing risks to the environment as well as the health of workers involved in the cleaning process. To address this problem, surfactant agents of a biodegradable nature and low toxicity, such as microbial surfactants, have been widely studied as an attractive, efficient solution to replace chemical surfactants in decontamination processes. In this work, the bacterial strains Pseudomonas cepacia CCT 6659, Pseudomonas aeruginosa UCP 0992, Pseudomonas aeruginosa ATCC 9027 and Pseudomonas aeruginosa ATCC 10145 were evaluated as biosurfactant producers in media containing different combinations and types of substrates and under different culture conditions. The biosurfactant produced by P. aeruginosa ATCC 10145 cultivated in a mineral medium composed of 5.0% glycerol and 2.0% glucose for 96 h was selected to formulate a biodetergent capable of removing heavy oil. The biosurfactant was able to reduce the surface tension of the medium to 26.40 mN/m, with a yield of approximately 12.00 g/L and a critical micelle concentration of 60.00 mg/L. The biosurfactant emulsified 97.40% and dispersed 98.00% of the motor oil. The detergent formulated with the biosurfactant also exhibited low toxicity in tests involving the microcrustacean Artemia salina and seeds of the vegetable Brassica oleracea. The detergent was compared to commercial formulations and removed 100% of the Special B1 Fuel Oil (OCB1) from different contaminated surfaces, demonstrating potential as a novel green remover with industrial applications.

Dyeing of bacterial cellulose films using plant-based natural dyes.

The aim of this work was to test the use of plant-based natural dyes on bacterial cellulose (BC) to add aesthetic value to dyed pellicles while maintaining the mechanical properties. Natural pigments from Clitoria ternatea L. and Hibiscus rosa-sinensis were tested. The commercial ARAQCEL RL 500 was also used for comparison purposes. The behavior of biocellulose regarding dye fixation, rehydration, tensile strength, and elasticity was evaluated in comparison to the dried biomaterial, showing that dyeing is a process that can be performed on hydrated BC. Dyeing the BC films through an innovative process maintained the crystallinity, thermal stability and mechanical strength of the BC and confirmed the compatibility of the membrane with the dyes tested, from the observed Scanning Electron Microscopy (SEM) morphology of nanofibers. Dyed biomaterial can be applied to various products, as confirmed by the results of the mechanical tests. As environmental awareness and public concern regarding pollution increase, the combination of natural dyes and BC pellicles can produce an attractive new material for the textile industry.