The pleiotropic role of Salinicoccus bacteria in enhancing ROS homeostasis and detoxification metabolism in soybean and oat to cope with pollution of triclosan.

Triclosan has been extensively used as a preservative in cosmetics and personal care products. However, its accumulation represents a real environmental threat. Thus, its phytotoxic impact needs more consideration. Our study was conducted to highlight the phytotoxic effect of triclosan on the growth, ROS homeostasis, and detoxification metabolism of two different plant species i.e., legumes (Glycine max) and grass (Avena sativa). Moreover, we investigated the potentiality of plant growth-promoting bacteria (ST-PGPB) in mitigating the phytotoxic effect of triclosan. Triclosan induced biomass (fresh and dry weights) reduction in both plants, but to a higher extent in oats. This decline was associated with a noticeable increment in the oxidative damage (e.g., MDA and H2O2) and detoxification metabolites such as metallothionein (MTC), phytochelatins (PCs), and glutathione-S-transferase (GST). This elevation was associated with a remarkable reduction in both enzymatic and non-enzymatic antioxidants. On the other hand, the bioactive strain of ST-PGPB, Salinicoccus sp. JzA1 significantly alleviated the harmful effect of triclosan on both soybean and oat plants by enhancing their biomass, photosynthesis, as well as levels of minerals (K, Ca, P, Mn, and Zn). In parallel, a striking quenching in oxidative damage and an obvious improvement in non-enzymatic (polyphenols, tocopherols, flavonoids) and enzymatic antioxidants were observed. Furthermore, Salinicoccus sp. JzA1 augmented the detoxification metabolism by enhancing the levels of phytochelatins, metallothionein, and glutathione-S-transferase (GST) activity in a species-specific manner which is more apparent in soybean rather than in oat plants. To this end, stress mitigating impact of Salinicoccus sp. JzA1 provides a basis to improve the resilience of crop species under cosmetics and personal care products toxicity.

How could actinobacteria augment the growth and redox homeostasis in barley plants grown in TiO2NPs-contaminated soils? A growth and biochemical study.

The increases in titanium dioxide nanoparticles (TiO2-NPs) released into the environment have raised concerns about their toxicity. However, their phytotoxic impact on plants is not well studied. Therefore, this study aimed at a deeper understanding of the TiO2-NPs phytotoxic impact on barley (Hordeum vulgare) growth and stress defense. We also hypothesized that soil inoculation with bioactive Rhodospirillum sp. JY3 strain can be applied as a biological fertilizer to alleviate TiO2-NPs phytotoxicity. At TiO2-NPs phytotoxicity level, photosynthesis was significantly retarded (∼50% reduction) in TiO2-NPs treated-barley plants which accordingly affect the biomass of barley plants. This retardation was accompanied by a remarkable induction of oxidative damage (H2O2, lipid peroxidation) with a concomitant reduction in the antioxidant defense metabolism. At a glance, Rhodospirillum sp. JY3 ameliorated the reduction in growth by enhancing the photosynthetic efficiency in contaminated barley plants. Moreover, Rhodospirillum sp. JY3 inoculation reduced the oxidative damage induced by TiO2-NPs via quenching H2O2 production and lipid peroxidation. Regarding the antioxidant defense arsenal, Rhodospirillum sp. JY3 enhanced both enzymatic (e.g. peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), …. etc.) and non-enzymatic (glutathione (GSH), ascorbate (ASC), polyphenols, flavonoids, tocopherols) antioxidants in shoots and to a greater extent roots of barley plants. Moreover, the inoculation significantly enhanced the heavy metal-detoxifying metabolites (eg. phytochelatins, glutaredoxin, thioredoxin, peroxiredoxin) as well as metal-detoxifying enzymes in barley shoots and more apparently in roots of TiO2-NPs stressed plants. Furthermore, there was an organ-specific response to TiO2-NPs and Rhodospirillum sp. JY3. To this end, this study shed light, for the first time, on the molecular bases underlie TiO2-NPs stress mitigating impact of Rhodospirillum sp. JY3 and it introduced Rhodospirillum sp. JY3 as a promising eco-friendly tool in managing environmental risks to maintain agricultural sustainability.

Effect of foliar application of nano-nutrients solution on growth and biochemical attributes of tomato (Solanum lycopersicum) under drought stress.

Introduction: Drought stress has drastically hampered the growth and yield of many crops. Therefore, environmentally safe agricultural techniques are needed to mitigate drought stress impact. To this end, foliar spray of nano-nutrients solution to (NNS) alleviate harmful aspects of drought stress. Methods: In a completely randomized design (CRD) experiment, seedlings were transplanted into pots at 2-3 leaf stage, each filled with loam-compost- organic manure soil (3:1:1). Plants were divided into two groups. (a) control group (b) applied stress group. Plants at vegetative stage were treated with 100% FC for control group and 60% FC for drought group, and these levels were maintained until harvesting. Three treatments of NNS with four levels i.e., 0%, 1%, 3% and 5% were given to all the pots after two weeks of drought stress treatment with a gap of 5 days at vegetative stage. Results and discussion: Application of 1% of nano-nutrient solution displayed an improvement in shoot length, shoot fresh and dry weight, number of leaves and flowers. Leaf chlorophylls and carotenoids and total phenolics contents were found maximum while minimum electrolyte leakage was observed at 3% application compared to control. Further, 1% application of NNS increased the Leaf RWC%, total soluble sugars, flavonoids contents. 5% NNS application exhibited higher total free amino acids with minimum lipid peroxidation rate in leaves of tomato under drought. Antioxidant enzyme activities increased in a concentration dependent manner as gradual increase was observed at 1%, 3% and 5%, respectively. Overall, this study introduced a new insights on using nano-nutrient solutions to maintain natural resources and ensure agricultural sustainability.

Future climate CO2 can harness ROS homeostasis and improve cell wall fortification to alleviate the hazardous effect of Phelipanche infection in pea seedlings.

Parasitic weeds such as Phelipanche aegyptiaca pose one of the most significant environmental constraints to cropping systems worldwide. The influence of P. aegyptiaca upon host plants is well studied, nevertheless, how future climate CO2 (eCO2) can affect P. aegyptiaca parasite-host interactions is not yet investigated. Considering the protective effect of eCO2, we studied its ability to mitigate the severity of P. aegyptiaca infection in pea plants (Pisum sativum). Our results revealed that Phelipanche infection strikingly reduced pea growth and photosynthesis. Moreover, infection with Phelipanche greatly burst the oxidative damage in pea plants by elevating photorespiration and NADPH oxidase activity. Contradictory, eCO2 extremely quenched the severity of P. aegyptiaca infection by diminishing the number and biomass of P. aegyptiaca tubercles. Additionally, eCO2 considerably mitigated the physiological and biochemical alterations exerted by Phelipanche upon pea seedlings. Within the physiological range, eCO2 augmented photosynthesis, that consequentially affected carbohydrate metabolism. Moreover, eCO2 highly mitigated the infection menace via quenching ROS overaccumulation which, sequentially reduced oxidative damage in infected pea plants. More interestingly, eCO2 improved cell wall fortification by enhancing lignin accumulation that considers the first line of defense against parasite penetration. Overall, this study concluded that pea plants grown in an atmosphere enriched with CO2 can efficiently cope with P. aegyptiaca infection via reducing Phelipanche tubercles, modulating ROS homeostasis, and enhancing cell wall fortification.