RESUMO
The experiment was conducted in male mice SBAchS57Vl/6 and Balb/c, which consumed water, obtained from the use of carbon nanotubes. in a free drinking regimen for 2 weeks (mice SBAchS57Vl/6) and 2 months (mice Balb/c) Control group consisted of three groups of animals: intact and mice received fine coal in the same concentrations as under the impact of the nanotubes. Under exposure to the maximal of the studied concentration of carbon nanotubes a significant change in the fine structure of the villi of the small intestine was found in the form of increasing the number of unstructured villi and proliferation of epithelial cells, most pronounced in duration of exposure until 2 months.
Assuntos
Proliferação de Células/efeitos dos fármacos , Mucosa Intestinal/efeitos dos fármacos , Intestino Delgado/efeitos dos fármacos , Nanotubos de Carbono/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Relação Dose-Resposta a Droga , Ingestão de Líquidos , Mucosa Intestinal/ultraestrutura , Intestino Delgado/ultraestrutura , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Nanotubos de Carbono/química , Propriedades de Superfície , Fatores de Tempo , Poluentes Químicos da Água/químicaRESUMO
The paper gives an assessment of the association of structural-functional (histological and cytological), cytogenetic, and cytotoxic changes in the analysis of various exposures of the body during both experiments (oral acrylamide) and population examinations (impact of pulp-and-paper mill waste on the nasal and oral mucosae). The assessment of the association of the study parameters is shown to serve to more completely objectify the pattern of actuating factors.
Assuntos
Acrilamida/toxicidade , Poluentes Atmosféricos/toxicidade , Indústria Química , Mucosa Bucal/efeitos dos fármacos , Mucosa Nasal/efeitos dos fármacos , Glândula Tireoide/efeitos dos fármacos , Administração Oral , Adolescente , Animais , Criança , Análise Citogenética , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/patologia , Humanos , Dose Letal Mediana , Contagem de Leucócitos , Leucócitos/efeitos dos fármacos , Leucócitos/patologia , Masculino , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Mucosa Bucal/patologia , Mucosa Nasal/patologia , Ratos , Glândula Tireoide/patologia , Glândula Tireoide/cirurgia , Tireoidectomia , Testes de ToxicidadeRESUMO
Experimental mutagenic effect of acrylamide on thyroid gland cells was studied by an extended micronucleus test. Acrylamide in doses corresponding to 0.004-0.1 LD(50) increased the incidence of thyroid follicular cells (A-cells) with micronuclei and other karyological parameters in exposed rats after hemithyroidectomy. This cytogenetic effect allows regarding acrylamide as a mutagen for the thyroid gland and as a carcinogen for this organ.
Assuntos
Acrilamida/toxicidade , Mutagênicos/toxicidade , Glândula Tireoide/efeitos dos fármacos , Animais , Masculino , Micronúcleos com Defeito Cromossômico , Ratos , Ratos WistarRESUMO
Long-term studies of 2 (cytohistology and genetic monitoring) laboratories of the Research Institute of Human Ecology and Environmental Hygiene, Russian Academy of Medical Sciences, to investigate the influence of environmental factors in the experiments made it possible to develop a noninvasive procedure for evaluating their cytological and cytogenetic impacts on man. A concurrent study of morphofunctional (histological, cytological) and cytogenetic parameters in both experimental and field trials can substantially extend and refine the interpretation of responses to environmental factors, which generates a need for using these parameters in hygienic and epidemiological studies.
