Enhancing tuberculosis vaccine development: a deconvolution neural network approach for multi-epitope prediction.

Tuberculosis (TB) a disease caused by Mycobacterium tuberculosis (Mtb) poses a significant threat to human life, and current BCG vaccinations only provide sporadic protection, therefore there is a need for developing efficient vaccines. Numerous immunoinformatic methods have been utilized previously, here for the first time a deep learning framework based on Deconvolutional Neural Networks (DCNN) and Bidirectional Long Short-Term Memory (DCNN-BiLSTM) was used to predict Mtb Multiepitope vaccine (MtbMEV) subunits against six Mtb H37Rv proteins. The trained model was used to design MEV within a few minutes against TB better than other machine learning models with 99.5% accuracy. The MEV has good antigenicity, and physiochemical properties, and is thermostable, soluble, and hydrophilic. The vaccine's BLAST search ruled out the possibility of autoimmune reactions. The secondary structure analysis revealed 87% coil, 10% beta, and 2% alpha helix, while the tertiary structure was highly upgraded after refinement. Molecular docking with TLR3 and TLR4 receptors showed good binding, indicating high immune reactions. Immune response simulation confirmed the generation of innate and adaptive responses. In-silico cloning revealed the vaccine is highly expressed in E. coli. The results can be further experimentally verified using various analyses to establish a candidate vaccine for future clinical trials.

Enhancing explainable SARS-CoV-2 vaccine development leveraging bee colony optimised Bi-LSTM, Bi-GRU models and bioinformatic analysis.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that caused the outbreak of the coronavirus disease 2019 (COVID-19). The COVID-19 outbreak has led to millions of deaths and economic losses globally. Vaccination is the most practical solution, but finding epitopes (antigenic peptide regions) in the SARS-CoV-2 proteome is challenging, costly, and time-consuming. Here, we proposed a deep learning method based on standalone Recurrent Neural networks to predict epitopes from SARS-CoV-2 proteins easily. We optimised the standalone Bidirectional Long Short-Term Memory (Bi-LSTM) and Bidirectional Gated Recurrent Unit (Bi-GRU) with a bioinspired optimisation algorithm, namely, Bee Colony Optimization (BCO). The study shows that LSTM-based models, particularly BCO-Bi-LSTM, outperform all other models and achieve an accuracy of 0.92 and AUC of 0.944. To overcome the challenge of understanding the model predictions, explainable AI using the Shapely Additive Explanations (SHAP) method was employed to explain how Blackbox models make decisions. Finally, the predicted epitopes led to the development of a multi-epitope vaccine. The multi-epitope vaccine effectiveness evaluation is based on vaccine toxicity, allergic response risk, and antigenic and biochemical characteristics using bioinformatic tools. The developed multi-epitope vaccine is non-toxic and highly antigenic. Codon adaptation, cloning, gel electrophoresis assess genomic sequence, protein composition, expression and purification while docking and IMMSIM servers simulate interactions and immunological response, respectively. These investigations provide a conceptual framework for developing a SARS-CoV-2 vaccine.

Effect of Gaussian filtered images on Mask RCNN in detection and segmentation of potholes in smart cities.

Accidents have contributed a lot to the loss of lives of motorists and serious damage to vehicles around the globe. Potholes are the major cause of these accidents. It is very important to build a model that will help in recognizing these potholes on vehicles. Several object detection models based on deep learning and computer vision were developed to detect these potholes. It is very important to develop a lightweight model with high accuracy and detection speed. In this study, we employed a Mask RCNN model with ResNet-50 and MobileNetv1 as the backbone to improve detection, and also compared the performance of the proposed Mask RCNN based on original training images and the images that were filtered using a Gaussian smoothing filter. It was observed that the ResNet trained on Gaussian filtered images outperformed all the employed models.

Local binary pattern and deep learning feature extraction fusion for COVID-19 detection on computed tomography images.

The deadly coronavirus virus (COVID-19) was confirmed as a pandemic by the World Health Organization (WHO) in December 2019. It is important to identify suspected patients as early as possible in order to control the spread of the virus, improve the efficacy of medical treatment, and, as a result, lower the mortality rate. The adopted method of detecting COVID-19 is the reverse-transcription polymerase chain reaction (RT-PCR), the process is affected by a scarcity of RT-PCR kits as well as its complexities. Medical imaging using machine learning and deep learning has proved to be one of the most efficient methods of detecting respiratory diseases, but to train machine learning features needs to be extracted manually, and in deep learning, efficiency is affected by deep learning architecture and low data. In this study, handcrafted local binary pattern (LBP) and automatic seven deep learning models extracted features were used to train support vector machines (SVM) and K-nearest neighbour (KNN) classifiers, to improve the performance of the classifier, a concatenated LBP and deep learning feature was proposed to train the KNN and SVM, based on the performance criteria, the models VGG-19 + LBP achieved the highest accuracy of 99.4%. The SVM and KNN classifiers trained on the hybrid feature outperform the state of the art model. This shows that the proposed feature can improve the performance of the classifiers in detecting COVID-19.