Drought in Portugal: Current regime, comparison of indices and impacts on extreme wildfires.

In Portugal, drought characterizes the climatic variability, contributes to the increase of fire risk and its duration and intensity are expected to increase in future climate. Surprisingly, the quantitative and objective analysis to characterize the drought regime in current climate conditions as well as its influence on the occurrence of large wildfires (LW) has never been done for Portugal, which are the main objectives of this study. We assessed drought regime for recent past climate conditions (1981-2017), using four different drought indices, namely SPI, SPEI, RDI and VCI, and assessed the influence of drought in LW occurrence. Results include the characterization of drought number, duration, severity, intensity, extension, intra- and inter-annual variability for different classes of severity and the space-time distribution of LW in drought periods and affected area. Our main findings include 67% of the study period were drought months; regions with higher drought duration and severity assessed with SPI and SPEI for general drought conditions evolves from north to south with the increase of drought assessment period; drought characteristics present low intra - annual and inter - annual variability but are clearly associated to the temporal and spatial distribution of LW. In fact, all LW occurred during drought assessed with SPI or SPEI, almost all LW (97% to 95%) and corresponding burnt area (98% to 97%) occurred during drought assessed with SPI and SPEI. The relationship between drought and fire incidence is statistical significant for 3 - month SPI, 3 - and 6 - month SPEI, and is particularly strong for Moderate and Severe drought. 85% and 87% of LW occurred in area affected by drought assessed with SPI or SPEI, respectively. It is not clear which is the best index, but drought plays a fundamental role in the occurrence of large wildfires in Portugal.

Heat waves in Portugal: Current regime, changes in future climate and impacts on extreme wildfires.

Heat waves (HW) can have devastating social, economic and environmental impacts. Together with long-term drought, they are the main factors contributing to wildfires. Surprisingly, the quantitative and objective analysis leading to the identification and characterization of HW in current and future climate conditions as well as its influence on the occurrence of extreme wildfires (EW) has never been performed for Portugal and are the main objectives of this study. For this reason, we assess HW in recent past and future climate based on a consistent high resolution meteorological database and have compared their occurrence with long and reliable, precise and detailed information about Portuguese fire events. Results include the characterization of HW frequency, duration, seasonality and intensity for current and different future climate conditions and their relationship with EW occurrence. We detected 130 HW between 1981 and 2010, concentrated between May and October and highest values in July and August. The highest HW number and duration is found over the Northeast corner and the south of the country while highest amplitudes are typically located in central area. HW characteristics present high inter-annual variability but are clearly associated to the temporal and spatial distribution of EW: 97% of total number of EW were active during an HW, 90% of total EW days were also HW days; 82% of the EW had duration completely contained in the duration of an HW; and, 83% of EW occurred during and in the area affected by HW. Our results also show that HW should increase in number, duration and amplitude, more significantly for RCP 8.5, and for the 30-year periods near the end of the 21st century. Findings of this study will support the definition of climate change adaptation strategies for fire danger and risk management.

Negligent and intentional fires in Portugal: Spatial distribution characterization.

In the European context, Portugal is the country with the highest number of wildfires and the second with more burnt area. The vast majority of these events are of human origin, whether caused by accident, negligence or arson, reason why it is particularly important to know the regime of these wildfires for forest and wildfire management activities. The study focuses on the most recent years of 2001-2014, when wildfire ignition's coordinates are known, and aims to identify and characterize the wildfire incidence spatial patterns of variability as well as their main drivers. After grouping wildfires with human cause into negligent and intentional, we studied their spatial distribution in terms of normalized number of wildfires (NNF) and burnt area (NBA) in the five Nomenclature of Territorial Units for Statistics II regions of mainland Portugal. Results disclose an uneven spatial distribution of the fire incidence, characterized by a south-north gradient, much higher values in Norte region and more evident for intentional than for negligent wildfires. Human and biophysical drivers strongly influence NNF and NBA, at regional and national scales. Distribution patterns at regional scale, for negligent and intentional wildfires are quite different from entire mainland and all wildfires. Drivers' influence is higher for intentional than for negligent wildfires, for southern than for northern regions and for NBA than for NNF. The leading drivers of NNF are distance to roads (d) population density (pd) and altitude (h) while of NBA are h, d, slope and pd, and this influence is higher for intentional than for negligent wildfires.

Screening of antimicrobial activity of macroalgae extracts from the Moroccan Atlantic coast.

The aim of this work is the screening of the antimicrobial activity of seaweed extracts against pathogenic bacteria and yeasts. The antimicrobial activity of the dichloromethane and ethanol extracts of ten marine macroalgae collected from the Moroccan's Atlantic coast (El-Jadida) was tested against two Gram+ (Bacillus subtilis and Staphylococcus aureus) and two Gram- (Escherichia coli and Pseudomonas aeruginosa) human pathogenic bacteria, and against two pathogenic yeasts (Candida albicans and Cryptococcus neoformans) using the agar disk-diffusion method. Seven algae (70%) of ten seaweeds are active against at least one pathogenic microorganisms studied. Five (50%) are active against the two studied yeast with an inhibition diameter greater than 15 mm for Cystoseira brachycarpa. Six (60%) seaweeds are active against at least one studied bacteria with five (50%) algae exhibiting antibacterial inhibition diameter greater than 15 mm. Cystoseira brachycarpa, Cystoseira compressa, Fucus vesiculosus, and Gelidium sesquipedale have a better antimicrobial activity with a broad spectrum antimicrobial and are a potential source of antimicrobial compounds and can be subject of isolation of the natural antimicrobials.

Anti-Candida and anti-Cryptococcus antifungal produced by marine microorganisms.

In order to search for antifungal from biological origin, we performed a screening of marine microorganisms isolated from seawater, seaweed, sediment and marine invertebrates collected from different coastal areas of the Moroccan Atlantic Ocean. The antifungal activities of these isolates were investigated against the pathogenic yeasts involved in medical mycology. Whole cultures of 34 marine microorganisms were screened for antifungal activities using the method of agar diffusion against four yeasts. The results showed that among the 34 isolates studied, 13 (38%) strains have antifungal activity against at least one out of four yeast species, 11 isolates have anti-Candida albicans CIP 48.72 activity, 12 isolates have anti-C. albicans CIP 884.65 activity, 13 isolates have anti-Cryptococcus neoformans activity and only 6 isolates are actives against Candida tropicalis R2 resistant to nystatin and amphotericin B. Nine isolates showed strong fungicidal activity. Fourteen microorganisms were identified and assigned to the genera Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Chromobacterium, Enterococcus, Pantoea, and Pseudomonas. Due to a competitive role for space and nutrient, the marine microorganisms could produce more antimicrobials; therefore these marine microorganisms were expected to be potential resources of natural products such as those we research: anti-Candida and anti-Cryptococcus fungicides.

Control of the radiative properties via photon-plasmon interaction in Er3+ -Tm3+ -codoped tellurite glasses in the near infrared region.

The novelty of this paper is that it reports on the tuning of the spectral properties of Er3+ -Tm3+ ions in tellurite glasses in the near-infrared region through the incorporation of silver or gold nanoparticles. These noble metal nanoparticles can improve the emission intensity and expand the bandwidth of the luminescence spectrum centered at 1535 nm, covering practically all the optical telecommunication bands (S, C + L and U), and extended up to 2010 nm wavelength under excitation by a 976 nm laser diode. Both effects are obtained by the combined emission of Er3+ and Tm3+ ions due to efficient energy transfer processes promoted by the presence of silver or gold nanoparticles for the (Er3+)4I(11/2)→(Tm3+)3H5, (Er3+)4I(13/2)→(Tm3+)3H4 and (Er3+)4I(13/2)→(Tm3+)3F4 transitions. The interactions between the electronic transitions of Er3+ and Tm3+ ions that provide a tunable emission are associated with the dynamic coupling mechanism described by the variations generated by the Hamiltonian H DC in either the oscillator strength or the local crystal field, i.e. the line shape changes in the near-infrared emission band. The Hamiltonian is expressed as eigenmodes associated with the density of the conduction electron generated by the different nanoparticles through its collective free oscillations at each resonance frequency of the nanoparticle and their geometric dependence. A complete description of photon-plasmon interactions of noble metal nanoparticles with the Er3+ and Tm3+ ions is provided.

Antifungal and antibacterial activity of marine microorganisms.

In order to explore marine microorganisms with pharmaceutical potential, marine bacteria, collected from different coastal areas of the Moroccan Atlantic Ocean, were previously isolated from seawater, sediment, marine invertebrates and seaweeds. The antimicrobial activities of these microorganisms were investigated against the pathogens involved in human pathologies. Whole cultures of 34 marine microorganisms were screened for antimicrobial activities using the method of agar diffusion against three Gram-positive bacteria, two Gram-negative bacteria, and against yeast. The results showed that among the 34 isolates studied, 28 (82%) strains have antimicrobial activity against at least one pathogen studied, 11 (32%) strains have antifungal activity and 24 (76%) strains are active against Gram-positive bacteria, while 21 (62%) strains are active against Gram-negative bacteria. Among isolates having antimicrobial activity, 14 were identified and were assigned to the genera Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Chromobacterium, Enterococcus, Pantoea and Pseudomonas. Due to a competitive role for space and nutrient, the marine microorganisms can produce antibiotic substance; therefore, these marine microorganisms were expected to be potential resources of natural antibiotic products.

Mid-infrared chalcogenide glass Raman fiber laser.

We report the first demonstration of a Raman fiber laser (RFL) emitting in the mid-infrared, above 3 µm. The operation of a single-mode As2S3 chalcogenide glass based RFL at 3.34 µm is demonstrated by using a low-loss Fabry-Pérot cavity formed by a pair of fiber Bragg gratings. A specially designed quasi-cw erbium-doped fluoride fiber laser emitting at 3.005 µm is used to pump the RFL. A laser output peak power of 0.6 W is obtained with a lasing efficiency of 39% with respect to the launched pump power.

Kappa casein gene polymorphism in local Tunisian goats.

The genetic polymorphism of the goat Kappa casein was investigated in Tunisian goats. Blood samples were collected from local goat breeds. Samples of genomic DNA were obtained from leukocytes of 175 dairy goats and regions of interest in the gene were amplified by Polymerase Chain Reaction (PCR) and then evaluated in agarose gel. For a better characterization of the single nucleotide polymorphism, a PCR-Restriction Fragment Length Polymorphism was performed employing the endonuclease DNA amplification using 459 bp primers. The PCR products of primers (459 bp) digested by restriction enzyme Alw44I produced two fragments of 459 and 381 bp. The Kappa casein allelic variants in tested animals revealed different genotypes, two of them were homozygous: AA or BB, AC or BC and CC. Genotypic frequencies were 12.5, 60.5 and 27% for AA or BB, CC and AC or BC, respectively. Identification of different variants of the Kappa casein can be used for the improvement and conservation of Tunisian local goats.

Writing of Bragg gratings through the polymer jacket of low-loss As2S3 fibers using femtosecond pulses at 800 nm.

Fiber Bragg gratings (FBG) were written through the polymer jacket of low-loss single mode As(2)S(3) chalcogenide fibers by using femtosecond laser pulses at 800 nm and a phase-mask. Peak reflectivity in excess of 99% was obtained at 3440 nm after 5 min of exposure. The resulting FBG maintained a peak reflectivity of 90% after 64 min of thermal annealing at 100 °C. This demonstration paves the way to the development of all-fiber mid-infrared laser sources.

Self-organized periodic structures on Ge-S based chalcogenide glass induced by femtosecond laser irradiation.

Self-organized periodic structures have been observed on the surface of the ablation craters of Ge-S based chalcogenide glass produced after irradiation by a focused beam of a femtosecond Ti:sapphire laser (1 kHz, 34 fs, 806 nm). Scanning electron microscopy and atomic force microscopy images of irradiated spots show a periodic structure of ripples with a spatial period of 720 nm (close to the wavelength of fs laser pulses) and an alignment parallel to the electric field of light. With an increasing number of pulses, from 5 to 50 pulses, a characteristic evolution of ripples was observed from a random structure to a series of generally aligned peaks-and-valleys self-organized periodic structures. Additionally, at the center of the ablated spot, micro-domains appear where the ripples are still regular but are assembled in a more complex fashion. The experimental observations are interpreted in terms of strong temperature gradients combined with interference of the incident laser irradiation and a scattered surface electromagnetic wave.

Low loss microstructured chalcogenide fibers for large non linear effects at 1995 nm.

Microstructured optical fibers (MOFs) are traditionally prepared using the stack and draw technique. In order to avoid the interfaces problems observed in chalcogenide glasses, we have developed a new casting method to prepare the chalcogenide preform. This method allows to reach optical losses around 0.4 dB/m at 1.55 µm and less than 0.05 dB/m in the mid IR. Various As(38)Se(62) chalcogenide microstructured fibers have been prepared in order to combine large non linear index of these glasses with the mode control offered by MOF structures. Small core fibers have been drawn to enhance the non linearities. In one of these, three Stokes order have been generated by Raman scattering in a suspended core MOF pumped at 1995 nm.

Microstructured chalcogenide optical fibers from As(2)S(3) glass: towards new IR broadband sources.

The aim of this paper is to present an overview of the recent achievements of our group in the fabrication and optical characterizations of As(2)S(3) microstructured optical fibers (MOFs). Firstly, we study the synthesis of high purity arsenic sulfide glasses. Then we describe the use of a versatile process using mechanical drilling for the preparation of preforms and then the drawing of MOFs including suspended core fibers. Low losses MOFs are obtained by this way, with background level of losses reaching less than 0.5 dB/m. Optical characterizations of these fibers are then reported, especially dispersion measurements. The feasibility of all-optical regeneration based on a Mamyshev regenerator is investigated, and the generation of a broadband spectrum between 1 µm and 2.6 µm by femto second pumping around 1.5 µm is presented.

Strong infrared spectral broadening in low-loss As-S chalcogenide suspended core microstructured optical fibers.

We report the fabrication and characterization of the first guiding chalcogenide As(2)S(3) microstructured optical fibers (MOFs) with a suspended core. At 1.55 microm, the measured losses are approximately 0.7 dB/m or 0.35 dB/m according to the MOF core size. The fibers have been designed to present a zero dispersion wavelength (ZDW) around 2 microm. By pumping the fibers at 1.55 microm, strong spectral broadenings are obtained in both 1.8 and 45-m-long fibers by using a picosecond fiber laser.

Characterization of the harmful effect of olive mill wastewater on spearmint.

In this study, changes in viability, biomass production, essential oil yield and essential oil composition of Mentha spicata L. (spearmint) exposed to olive mill wastewater (OMW) were investigated. Spearmint cuttings were sensitive to OMW and, after 6h of incubation in raw or diluted OMW, their viability was null. The short contact of raw OMW with mint cuttings caused an irreversible damage in rhizogenesis and shoots development. Roots were more sensitive to phytotoxicity than shoots. In a field essay, spearmint showed a good capability to recover when OMW was spread at 8 l m(-2) at the vegetative phase of growth (45 days after plantation). At this dose, a slight increase of mostly of the mint essential oil constituents was obtained. When the dose applied was 16 l m(-2), phytotoxicity was manifested by a high reduction of biomass and essential oil yield. The essential oil composition was also affected and a disappearance of many of mint essential oil constituents was observed with an increase of 59% for carvone, the major compound of spearmint essential oil. As far as we know, this is the first report on the effect of field application of OMW on an aromatic plant essential oil yield and composition.

Tuberculous duodenal stenosis: report of two cases.

Duodenal tuberculosis is a rare clinical entity. The authors report and emphasize the lack of special clinical, radiological and endoscopic signs of duodenal tuberculosis. The diagnosis is affirmed, at laparotomy, out of the findings of peritoneal granulations or histology of lymphatic nodes. We report our experience of two cases of duodenal tuberculosis presenting with proximal intestinal obstruction and review the available literature.